UPLC-Q-TOF-MS~E技术结合UNIFI数据库快速定性分析补气通络颗粒的化学成分
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摘要
目的应用超高效液相色谱联用四级杆飞行时间质谱仪(UPLC-Q-TOF-MS~E)结合UNIFI数据平台对补气通络颗粒的化学成分进行快速定性分析。方法应用时间依赖型MS~E法采集样品质谱数据,结合自动检出及人工核对方法分析补气通络颗粒的化学成分。结果通过UNIFI数据库自动检测结果、搜索在线和离线数据库以及人工核对相关文献,鉴定黄酮类、皂苷类、萜类、酯类、氨基酸和酚酸类共72种化合物。其中,7种来自红芪,14种来自三七,24种来自泽泻,9种来自当归,2种来自川芎,6种来自蝉蜕,6种来自郁金,4种来自桂枝。结论本方法可快速定性分析补气通络颗粒的多种化学成分,为补气通络颗粒的质量控制、药效物质基础解析、作用机制阐明和组分配伍中药的开发提供参考。
Objective To rapidly and qualitatively analyze the chemical constituents of Buqi Tongluo Granules by using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS~E)combining UNIFI data platform.Methods The mass spectrometry data were collected by time-dependent MS~Eacquisition mode.The chemical constituents of Buqi Tongluo Granules were analyzed by automatic detection and artificial checking.Results Through automated test by UNIFI database,searching online and offline databases and manually checking related literature,72 compounds were identified including flavonoids,saponins,terpenoids,esters,amino acids and phenolic acids.Among them,7 compounds from Radix hedysari,14 compounds fromPanax notoginseng,24 compounds from Alisma orientalis,9 compounds from Angelica sinensis,2 compounds from Chuanxiong,6 compounds fromCicada moult,6 compounds fromRadix curcumae and 4 compounds fromCinnamon twig.Conclusion This method can quickly and qualitatively analyze the chemical constituents of Buqi Tongluo Granules,meanwile,the reaserch can also provide reference for the quality control of Buqi Tongluo Granules,the analysis of pharmacodynamic substances,the elucidation of action mechanism and the development component compatibility of Chinese herbal medicine.
Objective To rapidly and qualitatively analyze the chemical constituents of Buqi Tongluo Granules by using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS~E)combining UNIFI data platform.Methods The mass spectrometry data were collected by time-dependent MS~Eacquisition mode.The chemical constituents of Buqi Tongluo Granules were analyzed by automatic detection and artificial checking.Results Through automated test by UNIFI database,searching online and offline databases and manually checking related literature,72 compounds were identified including flavonoids,saponins,terpenoids,esters,amino acids and phenolic acids.Among them,7 compounds from Radix hedysari,14 compounds fromPanax notoginseng,24 compounds from Alisma orientalis,9 compounds from Angelica sinensis,2 compounds from Chuanxiong,6 compounds fromCicada moult,6 compounds fromRadix curcumae and 4 compounds fromCinnamon twig.Conclusion This method can quickly and qualitatively analyze the chemical constituents of Buqi Tongluo Granules,meanwile,the reaserch can also provide reference for the quality control of Buqi Tongluo Granules,the analysis of pharmacodynamic substances,the elucidation of action mechanism and the development component compatibility of Chinese herbal medicine.
引文
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[3]杨岱琳,佟玲,李晓稳,等.UPLC-Q-TOF/MSE方法分析养血清脑颗粒的化学成分[J].药学学报,2016,51(5):797-805.
[4]李金花,曾锐,瞿燕,等.UPLC-Q-TOF-MSE技术结合UNIFI数据库快速定性分析黄牡丹化学成分[J].中草药,2017,48(8):1529-1536.
[5]杨林军,谢彦云,李志锋,等.UPLC-Q-TOF-MS/MS分析中华常春腾中的化学成分[J].中草药,2016,47(4):566-572.
[6]Zhang F,Li M,Qiao L,et al.Rapid characterization of Ziziphi Spinosae Semen by UPLC/Qtof MS with novel informatics platform and its application in evaluation of two seeds fromZiziphus species[J].J Pharm Biomed A-nal,2016,122:59-80.
[7]卢新义,何娟,陈衍斌,等.超高效液相色谱-四级杆-飞行时间串联质谱法同时快速鉴定复方延寄参胶囊的化学成分[J].西北药学杂志,2018,33(2):181-185.
[8]闫利利,史家文,王金芳,等.基于UPLC-Q-TOF-MSE方法分析半夏泻心汤的化学成分[J].药学学报,2013,48(4):526-531.
[9]牛江涛,曹瑞,司昕蕾,等.不同密炙方法对红芪中毛蕊异黄酮和芒柄花素含量的影响[J].中国中医药信息杂志,2017,24(9):59-62.
[10]王莹,禇扬,李伟,等.三七中皂苷成分及其药理作用的研究进展[J].中草药,2015,46(9):1381-1392.
[11]孙莹莹,刘玥,陈可冀.人参皂苷的心血管药理效应:进展与思考[J].中国科学:生命科学,2016,46(6):771-778.
[12]邢增智,陈旺,曾宇.泽泻的化学成分与药理作用研究进展[J].中医药导报,2017,23(15):75-78.
[13]汪春飞,成旭东,顾俊菲,等.泽泻化学物质基础及其毒性研究进展[J].中国中药杂志,2015,40(5):840-846.
[14]张来宾,吕洁丽,陈红丽,等.当归中苯酞类成分及药理作用研究进展[J].中国中药杂志,2016,41(2):167-176.
[15]杨璐,李国玉,王金辉.蝉蜕的化学成分和药理作用的研究现状[J].农垦医学,2011,33(2):184-186.
[16]吴国霞,杨秀娟,邓毅,等.当归不同药用部位水煎液指纹图谱及成分分析[J].中成药,2018,40(4):890-894.
[17]杨银花,赵博琛,兰颐,等.当归酚酸类成分提取、富集工艺研究[J].北京中医药大学学报,2013,36(9):627-632.
[18]何婷,尚靖.药物多组分、多靶点研究进展[J].亚太传统医药,2011,7(7):166-167.
[19]张威,刘嘉,杨海军,等.多组分中药整体性质的表征研究概述[J].中医药信息,2015,32(2):125-127.
[20]何群,汪宁,段金廒,等.中药及复方的药效物质基础研究概况[J].亚太传统医药,2009,5(6):37-40.
[21]徐硕,姜文清,邝咏梅,等.细胞膜色谱技术应用于中药活性成分筛选的研究进展[J].西北药学杂志,2018,33(2):274-277.
[22]钟敬华.基于液质联用技术的脑得生片和胃复春片物质基础辨识方法研究[D].杭州:浙江大学,2010:1-63.