基于近红外光谱技术对山茱萸炮制程度的质量评价
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摘要
目的采用近红外光谱技术建立山茱萸饮片中5-羟甲基糠醛含量的快速无损检测方法,并用于快速判别山茱萸饮片的炮制程度。方法以54个样本作为校正集,运用偏最小二乘法建立定量校正模型并进行优化。以9个样本作为验证集,进行含量预测。结果校正模型决定系数为0.925 6,相对分析误差为5.06,交叉验证均方根误差为0.022 0,外部验证均方差为0.019 3,光谱预测值与真值之间的相关性较好。结论该法可直接用于中药山茱萸中5-羟甲基糠醛含量的快速预测,为快速判断山茱萸炮制程度提供可能。该方法具有操作简便、快速无损、准确可靠等优点。
OBJECTIVE To establish a fast and nondestructive detection method on the content of 5-hydroxymethylfurfural(5-HMF) in Corni Fructus by near infrared spectrometry, and used to identify the processing degree rapidly. METHODS The quantitative model was built based on partial least square method with 54 samples, and then the models were optimized. Nine samples were used for content prediction. RESULTS The determination coefficient of the calibration models was 0.925 6, ratio of prediction to deviation was 5.06, root mean square error of cross validation was 0.022 0, and root mean square error of prediction was 0.019 3, these results indicated the correlation between the predicted values and the true values was good.CONCLUSION This method can be applied in rapid quantitative analysis of 5-HMF in the samples of Corni Fructus and identification of their processing degree. The method has the advantages of simple, rapid, nondestructive, accurate and safety.
OBJECTIVE To establish a fast and nondestructive detection method on the content of 5-hydroxymethylfurfural(5-HMF) in Corni Fructus by near infrared spectrometry, and used to identify the processing degree rapidly. METHODS The quantitative model was built based on partial least square method with 54 samples, and then the models were optimized. Nine samples were used for content prediction. RESULTS The determination coefficient of the calibration models was 0.925 6, ratio of prediction to deviation was 5.06, root mean square error of cross validation was 0.022 0, and root mean square error of prediction was 0.019 3, these results indicated the correlation between the predicted values and the true values was good.CONCLUSION This method can be applied in rapid quantitative analysis of 5-HMF in the samples of Corni Fructus and identification of their processing degree. The method has the advantages of simple, rapid, nondestructive, accurate and safety.
引文
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[2]LIANG J R.Research advances in chemical constituents and biological activity of Fructus corni[D].Xi’an:Northwest University,2014.
[3]ZHANG L N,ZHANG T L,JIN G Q,et al.Protein expression of P-synapsinⅠfollowing 5-HMF of SHUDI treatment in hippocampal neurons with corticosterone injury[J].Chin JGerontol(中国老年学杂志),2015,35(9):2305-2307.
[4]GUAN L B,LI X X,LI J S,et al.Effect of 5-HMF on hemoglobin-oxygen affinity,swimming endurance and spatial memory ability of rats in high altitude simulated environment[J].J Third Mil Med Univ(第三军医大学学报),2015,37(15):1561-1565.
[5]ZOU L,QIU B X,LIU K,et al.Content of polysaccharide and5-hydroxymethylfurfural(5-HMF)in Codonopsis Radix after cooked with rice and its impact on rabbit gastrointestinal smooth muscle in vitro[J].Chin Tradit Herbal Drugs(中草药),2017,48(1):149-154.
[6]LIU Y Q,XU C X,JIA R,et al.Effects of three compounds separated from Atractylodes lancea(Thunb.)DC on Spleen disorder model rats[J].Chin Arch Tradit Chin Med(中华中医药学刊),2017,35(2):382-386.
[7]LIU Y,CHEN H F,ZHU Y P,et al.On the mechanism of processing cornus officinalis with wine by high pressure[J].JBengbu Med Coll(包头医学院学报),2017,33(1):115-117.
[8]CHANG Z R,LI J,HAO B,et al.Analysis of characteristic chemical components in crude and processed Fructus Corni[J].Chin J Pharm Anal(药物分析杂志),2015,35(2):338-343.
[9]PAN Z,SHAO H X,LIU T,et al.Research progress of5-hydroxymethylfurfural,a safety-related substance in traditional Chinese medicine injections[J].Chin J Chin Mater Med(中国中药杂志),2017,42(10):1842-1846.
[10]YING X H,LI J H,LIU L,et al.Rapid analysis of multi-components of Ginseng Radix et Rhizoma Rubra based on near infrared spectroscopy[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(10):1377-1384.
[11]ZHAO Y L,ZHANG J,YUAN T J,et al.Study on rapid identification of medicinal plants of paris polyphylla from different origin areas by NIR spectroscopy[J].Spectrosc Spect Anal(光谱学与光谱分析),2014,34(7):1831-1835.
[12]XUE S J,CHEN S Q,WANG L L,et al.Qualitative and quantitative study on different commercial specifications of Radix Rehmanniae based on near infrared spectroscopy[J].Chin J Tradit Chin Med(中华中医药杂志),2017,32(5):2055-2059.
[13]KUANG Y H,TANG H J,WANG D Q,et al.A Method for analyzing uniformity of borneol in manufactory process of compound Danshen tablets by near infrared spectroscopy[J].Chin J Tradit Med Form(中国实验方剂学杂志),2018,24(6):7-11.
[14]WU L M.Study on rapid detection for the quality of some herbal medicines and Chinese patent medicines by near infrared spectroscopy[D].Chongqing:Southwest University,2013.