食源性霉菌和酵母菌检测技术进展
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摘要
真菌广泛存在于空气、水、各种动植物体和有机体中,在生长过程中产生容易引起人和动物生理变化和病理变化的次级代谢产物,导致人类和动物的急性或慢性中毒。因此,快速准确地检测食品中污染真菌已成为保障食品安全和预防疾病的重要环节。污染食品的真菌主要是霉菌和酵母菌,对其检测方法有多种,但不同检测方法各有其优势和不足以及不同的应用范围,就近年来食源性霉菌和酵母菌检验方法作一简要概述。
Fungi widely exist in air,water,plants and animals.They produce secondary metabolites during growth,which are easy to cause physiological and pathological changes in humans and animals,resulting in acute or chronic poisoning.Rapid and accurate detection for fungi in contaminated food has become an important measure to ensure food safety and prevent disease.Fungi that cause food contamination are mainly molds and yeasts,and there are many detection methods for them.However,different detection methods have their own advantages and disadvantages as well as different scope of application.In this paper,the detection methods for foodborne molds and yeasts were reviewed.
Fungi widely exist in air,water,plants and animals.They produce secondary metabolites during growth,which are easy to cause physiological and pathological changes in humans and animals,resulting in acute or chronic poisoning.Rapid and accurate detection for fungi in contaminated food has become an important measure to ensure food safety and prevent disease.Fungi that cause food contamination are mainly molds and yeasts,and there are many detection methods for them.However,different detection methods have their own advantages and disadvantages as well as different scope of application.In this paper,the detection methods for foodborne molds and yeasts were reviewed.
引文
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[20] 扈庆华,郑薇薇,石晓路,等.改良分子信标-实时PCR快速检测副溶血弧菌[J].现代预防医学,2004(03):441-443.
[21] 李金明.实时荧光PCR技术[M].北京:人民军医出版社,2007:28.
[22] TOMLINSON J,BOONHAMN.Potential of LAMP for detection of plant pathogens[J].CAB Review:perspectives in Agriculture,Veterinary Science,Nutrition and Natural Resources,2008,3(66):1-7.
[23] LIU P,LI B,YIN R,et al.Development and evaluation of ITS-and aflP-based LAMP assays for rapid detection of Aspergillus flavus in food samples[J].Can J Microbiol,2014,60(09):579-584.
[24] NAKAYAMA T,YAMAZAKI T,YO A,et al.Detection of fungi from an indoor environment using loop-mediated isothermal amplification(LAMP)Method[J].Biocontrol Science,2017,22(02):97-104.
[25] 张小飞,白杰英,赵爽,等.环介导等温扩增技术的研究进展和微生物检测应用[J].实验动物与比较医学,2011,31(03):226-230.
[26] 张丽娜,胡成进.环介导等温扩增技术在病原微生物快速诊断中的应用[J].生物技术通讯,2015,26(06):886-890.
[27] 樊蕴秀,李敏.实时光电微生物检测技术在乳品质控中的应用[J].乳业科学与技术,2013,36(05):25-28.
[28] 刘道亮,胡连霞,赵占民,等.流式细胞技术快速检测果汁中的霉菌、酵母菌[J].食品工业科技,2011(08):387-391.
[29] FERREIRA A M,CRUZ-MOREIRA D,CERQUEIRA L,et al.Yeasts identification in microfluidic devices using peptide nucleic acid fluorescence in situ hybridization(PNA-FISH)[J].Biomedical Microdevices,2017,19(01):11.
[30] 刘鹏,刘宁,王云霞,等.肽核酸探针在微生物快速检测中的应用[J].食品安全质量检测学报,2016,7(04):1363-1368.
[31] QUINTILLA R,KOLECKA A,CASAREGOLA S,et al.MALDI-TOF MS as a tool to identify foodborne yeasts and yeast-like fungi.[J].International Journal of Food Microbiology,2017(266):109.
[32] 饶名祯,顾晨荣,李云霞,等.MALDI-TOF MS技术在食品微生物领域的应用研究[J].上海师范大学学报(自然科学版),2015,44(06):681-686.
[33] ZHOU C,HU B,ZHANG X,et al.The value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in identifying clinically relevant bacteria:a comparison with automated microbiology system[J].Journal of Thoracic Disease,2014,6(05):545.
[34] LI Y,GU B,LIU G,et al.MALDI-TOF MS versus VITEK 2 ANC card for identification of anaerobic bacteria[J].Journal of Thoracic Disease,2014,6(05):517-523.
[35] 周千渝,张延国,黄成才,等.食源性致病菌MALDI-TOF MS检测方法的建立与应用[J].食品工业科技,2015,36(18):59-64+76.
[36] KRISHNAMURTHY T,ROSS P L.Rapid identification of bacteria by direct matrix-assisted laser desorption/ionization mass spectrometric analysis of whole cells[J].Rapid Communications in Mass Spectrometry,1996,10(15):1992-1996.
[2] 周威,胡梁斌,李红波,等.食物中食源性病原菌检测技术研究进展[J].食品研究与开发,2017(09):213-216.
[3] PENG L X.Rapid detection of yeast and mold in filterable beverage using PCR assay[J].Annual Meeting,2012(59):3.
[4] 周玉庭,任佳丽,张紫莺.粮食中霉菌污染检测方法现状及发展趋势[J].食品安全质量检测学报,2016,7(01):244-250.
[5] 孙秀兰,蒋栋磊,张银志.生物传感器应用于食源性致病菌检测研究进展[J].中国微生态学杂志,2011,23(11):1040-1042.
[6] 王发园,陈欣.食品的微生物污染[J].农学学报,2009(04):86-87.
[7] FERGUSON B S.A look at the microbiology testing market[J].Food Safety Magazine,2017(46):14-15.
[8] 马群飞.GB 4789.15—1016《食品安全国家标准:食品微生物学检验霉菌和酵母计数》标准解读[J].中国卫生标准管理,2018(05):1-3.
[9] 郝三虎,徐应根,石茂林.纸片法和国标法对食品霉菌检验效果比较[J].现代医药卫生,2014,30(23):3554-3555.
[10] TERAMURA H,USHIYAMA M,OGIHARA H,et al.Evaluation of a novel dry sheet culture method(Sanita-kun(R))for rapid enumeration of yeasts and molds in foods[J].Journal of Microbiological Methods,2015(109):16-19.
[11] BIRD P,FLANNERY J,CROWLEY E,et al.Evaluation of the 3M petrifilm rapid yeast and mold count plate for the enumeration of yeast and mold in food:collaborative study,first action 2014.05[J].Journal of Aoac International,2015,98(03):767-783.
[12] 丛苑,卢彦廷,杜奕君,等.ATP发光法快速检测玉米中的霉菌[J].中国食品学报,2014,14(08):233-239.
[13] 李艳霞,吴松浩.食品微生物检测技术的研究进展[J].食品工业科技,2008,29(07):270-273.
[14] 黄吉城,赖蔚冬,戴昌芳,等.电阻抗法快速测定饮用纯净水中细菌和真菌总数的研究[J].中国卫生检验杂志,2001,11(01):19-20.
[15] MADDEN R H,GILMOUR A.Impedance as an alternative to MPN enumeration of coliforms in pasteurized milks[J].Letters in Applied Microbiology,1995,21(06):387.
[16] 敖特根巴雅尔,乌兰其其格,陆继爽,等.干肉制品中霉菌PCR鉴定方法的建立[J].畜牧与饲料科学,2016,37(03):49-51.
[17] BLEVE G,RIZZOTTI L,DELLAGLIO F,et al.Development of reverse transcription(RT)-PCR and real-time RT-PCR assays for rapid detection and quantification of viable yeasts and molds contaminating yogurts and pasteurized food products[J].Applied & Environmental Microbiology,2003,69(07):4116-4122.
[18] 万松华.食品中污染微生物通用型荧光PCR检测方法研究[J].现代食品科技,2014,30(12):215-219.
[19] 崔溢,张贵海.实时荧光定量PCR法检测乳制品中的霉菌和酵母探究[C]//宋昆冈.2014中国乳制品工业协会第二十次年会论文集,2014中国乳制品工业协会第二十次年会,上海:中国乳制品工业协会,2014:170-172.
[20] 扈庆华,郑薇薇,石晓路,等.改良分子信标-实时PCR快速检测副溶血弧菌[J].现代预防医学,2004(03):441-443.
[21] 李金明.实时荧光PCR技术[M].北京:人民军医出版社,2007:28.
[22] TOMLINSON J,BOONHAMN.Potential of LAMP for detection of plant pathogens[J].CAB Review:perspectives in Agriculture,Veterinary Science,Nutrition and Natural Resources,2008,3(66):1-7.
[23] LIU P,LI B,YIN R,et al.Development and evaluation of ITS-and aflP-based LAMP assays for rapid detection of Aspergillus flavus in food samples[J].Can J Microbiol,2014,60(09):579-584.
[24] NAKAYAMA T,YAMAZAKI T,YO A,et al.Detection of fungi from an indoor environment using loop-mediated isothermal amplification(LAMP)Method[J].Biocontrol Science,2017,22(02):97-104.
[25] 张小飞,白杰英,赵爽,等.环介导等温扩增技术的研究进展和微生物检测应用[J].实验动物与比较医学,2011,31(03):226-230.
[26] 张丽娜,胡成进.环介导等温扩增技术在病原微生物快速诊断中的应用[J].生物技术通讯,2015,26(06):886-890.
[27] 樊蕴秀,李敏.实时光电微生物检测技术在乳品质控中的应用[J].乳业科学与技术,2013,36(05):25-28.
[28] 刘道亮,胡连霞,赵占民,等.流式细胞技术快速检测果汁中的霉菌、酵母菌[J].食品工业科技,2011(08):387-391.
[29] FERREIRA A M,CRUZ-MOREIRA D,CERQUEIRA L,et al.Yeasts identification in microfluidic devices using peptide nucleic acid fluorescence in situ hybridization(PNA-FISH)[J].Biomedical Microdevices,2017,19(01):11.
[30] 刘鹏,刘宁,王云霞,等.肽核酸探针在微生物快速检测中的应用[J].食品安全质量检测学报,2016,7(04):1363-1368.
[31] QUINTILLA R,KOLECKA A,CASAREGOLA S,et al.MALDI-TOF MS as a tool to identify foodborne yeasts and yeast-like fungi.[J].International Journal of Food Microbiology,2017(266):109.
[32] 饶名祯,顾晨荣,李云霞,等.MALDI-TOF MS技术在食品微生物领域的应用研究[J].上海师范大学学报(自然科学版),2015,44(06):681-686.
[33] ZHOU C,HU B,ZHANG X,et al.The value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in identifying clinically relevant bacteria:a comparison with automated microbiology system[J].Journal of Thoracic Disease,2014,6(05):545.
[34] LI Y,GU B,LIU G,et al.MALDI-TOF MS versus VITEK 2 ANC card for identification of anaerobic bacteria[J].Journal of Thoracic Disease,2014,6(05):517-523.
[35] 周千渝,张延国,黄成才,等.食源性致病菌MALDI-TOF MS检测方法的建立与应用[J].食品工业科技,2015,36(18):59-64+76.
[36] KRISHNAMURTHY T,ROSS P L.Rapid identification of bacteria by direct matrix-assisted laser desorption/ionization mass spectrometric analysis of whole cells[J].Rapid Communications in Mass Spectrometry,1996,10(15):1992-1996.