文山兜兰种子非共生萌发方法的研究
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摘要
以文山兜兰成熟但未开裂的果荚为研究材料,以种子萌发时间及萌发率作为考察指标,探究固体培养和液体悬浮培养2种方法对文山兜兰种子萌发的影响,同时采用单因素法探究直接播种与低温处理播种对文山兜兰种子萌发的影响。结果表明,与固体培养方法相比,文山兜兰种子在液体悬浮培养的条件下平均萌发时间提前且萌发率更高,液体悬浮培养更适合文山兜兰种子初期的萌发培养。文山兜兰种子直接播种,其萌发时间较长,且萌发率较低。在经过低温处理73d以后再进行播种,文山兜兰种子萌发时间可提前13d,萌发率达到59.33%。不同的培养基种类能显著地影响文山兜兰种子萌发的时间及萌发率,1g/L的花宝1号(含椰子粉)培养基能显著缩短萌发的时间,并且具有较高的萌发率。综合分析,文山兜兰种子最佳的萌发方法为低温处理种子,采用花宝1号(含椰子粉)作为培养基进行液体悬浮培养。
Based on seed germination time and germination rate as indicators by using mature but un-cracked fruit pods of Paphiopedilum wenshanense as materials,the effects of solid culture and liquid suspension culture on the P.wenshanense seed germination were studied,and the effect of direct sowing and low temperature treatment were also analyzed through single factor process.The results showed that compared with the solid culture method,the average germination time and the germination rate of P.wenshanense seeds were higher in liquid suspension culture conditions,and the liquid suspension culture was more suitable for the initial germination culture of P.wenshanense.The time required for P.wenshanense seeds germination,cultured after direct sowing,was longer and the germination rate was lower.After soaking for 73 days by low temperature treatment,the seed germination time was 13 d earlier and the germination rate reached 59.33%.Different media types could significantly affect the seed germination time and seed germination rate of P.wenshanense.The 1 g/L hyponex No.1(with coconut powder)medium could significantly reduce the germination time with high germination rate.In conclusion,the best germination condition for was low temperature seeds treatment by using hyponex No.1(containing coconut powder)as a medium for liquid suspension culture.
Based on seed germination time and germination rate as indicators by using mature but un-cracked fruit pods of Paphiopedilum wenshanense as materials,the effects of solid culture and liquid suspension culture on the P.wenshanense seed germination were studied,and the effect of direct sowing and low temperature treatment were also analyzed through single factor process.The results showed that compared with the solid culture method,the average germination time and the germination rate of P.wenshanense seeds were higher in liquid suspension culture conditions,and the liquid suspension culture was more suitable for the initial germination culture of P.wenshanense.The time required for P.wenshanense seeds germination,cultured after direct sowing,was longer and the germination rate was lower.After soaking for 73 days by low temperature treatment,the seed germination time was 13 d earlier and the germination rate reached 59.33%.Different media types could significantly affect the seed germination time and seed germination rate of P.wenshanense.The 1 g/L hyponex No.1(with coconut powder)medium could significantly reduce the germination time with high germination rate.In conclusion,the best germination condition for was low temperature seeds treatment by using hyponex No.1(containing coconut powder)as a medium for liquid suspension culture.
引文
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[16]李秀玲,黄昌艳,宋倩,等.同色兜兰的非共生萌发与快速繁殖研究[J].植物科学学报,2016,34(1):127-134.
[17]陈莹,范旭丽,高江云.白旗兜兰种子非共生萌发与试管苗快速繁育[J].植物分类与资源学报,2015,37(5):611-615.
[18]Fracchia S,Aranda-Rickert A,Rothen C, et al.Associated fungi,symbiotic germination and in vitro seedling development of the rare Andean terrestrial orchid Chloraea riojana[J].Flora,2016,224:106-111.
[19]黄玮婷,曾宋君.文山兜兰白变种的无菌播种和试管成苗[J].植物生理学通讯,2010,46(10):1069-1070.
[20]Lee Y I.The asymbiotic seed germination of six Paphiopedilum species in relation to the time of seed collection and seed pretreatment[C].International Conference on Quality Management in Supply Chains of Ornamentals,2007,755:381-386.
[21]田凡,姜运力,罗在柒,等.白花兜兰种子无菌萌发及试管成苗技术研究[J].贵州林业科技,2014,42(3):34-38.
[2]王贞,丛磊,刘燕.兜兰属植物研究现状[J].林业科学,2006,42(7):113-119.
[3]陈心启,刘方媛.云南几种兜兰属植物[J].云南植物研究,1982,4(2):16-17.
[4]龙波,龙春林.兜兰属植物及其研究现状[J].自然杂志,2006,28(6):341-344.
[5]曾宋君,夏念和,陈之林,等.国产兜兰属植物观赏价值评价及其在华南地区的应用前景分析[J].中国野生植物资源,2011,30(2):9-13,35.
[6]朱亚艳,王港,侯娜,等.贵州南部野生兜兰SRAP遗传多样性分析[J].西南林业大学学报,2017,37(1):10-14.
[7]曾宋君,陈砚,吴坤林,等.国外兜兰属植物的引种和栽培初报[J].中国野生植物资源,2013,32(5):62-68.
[8]曾宋君,田瑞雪,陈之林,等.兜兰属植物杂交育种研究进展[J].热带亚热带植物学报,2010,18(4):459-468.
[9]曾宋君,陈之林,吴坤林,等.国产兜兰属植物的引种和栽培[J].中国野生植物资源,2010,29(2):53-58.
[10]曾宋君,陈之林,吴坤林,等.兜兰无菌播种和组织培养研究进展[J].园艺学报,2007,45(3):793-796.
[11]曾宋君,陈之林,段俊.带叶兜兰的无菌播种和离体快速繁殖[J].植物生理学通讯,2006,42(2):247.
[12]崔莹莹,郑福山,陈昆,等.霍山石斛微体快繁技术研究[J].西部林业科学,2017,46(6):51-55.
[13]Jiang H,Chen M C,Lee Y I, et al.In vitro germination and low-temperature seed storage of Cypripedium lentiginosum P.J.Cribb & S.C.Chen,a rare and endangered lady's slipper orchid[J].Scientia Horticulturae,2017,225:471-479.
[14]Li Y,Yang Z L,Ia S Y, et al.Mycorrhizal specificity,preference,and plasticity of six slipper orchids from South Western China[J].Mycorrhiza,2010,20(8):559-568.
[15]张敬虎,康红涛,邹金美,等.兜兰无菌播种与组培技术研究进展[J].福建热作科技,2017,42(2):61-64.
[16]李秀玲,黄昌艳,宋倩,等.同色兜兰的非共生萌发与快速繁殖研究[J].植物科学学报,2016,34(1):127-134.
[17]陈莹,范旭丽,高江云.白旗兜兰种子非共生萌发与试管苗快速繁育[J].植物分类与资源学报,2015,37(5):611-615.
[18]Fracchia S,Aranda-Rickert A,Rothen C, et al.Associated fungi,symbiotic germination and in vitro seedling development of the rare Andean terrestrial orchid Chloraea riojana[J].Flora,2016,224:106-111.
[19]黄玮婷,曾宋君.文山兜兰白变种的无菌播种和试管成苗[J].植物生理学通讯,2010,46(10):1069-1070.
[20]Lee Y I.The asymbiotic seed germination of six Paphiopedilum species in relation to the time of seed collection and seed pretreatment[C].International Conference on Quality Management in Supply Chains of Ornamentals,2007,755:381-386.
[21]田凡,姜运力,罗在柒,等.白花兜兰种子无菌萌发及试管成苗技术研究[J].贵州林业科技,2014,42(3):34-38.