超声降解落叶松中阿拉伯半乳聚糖及其活性的研究
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摘要
以落叶松[Larix gmelinii(Rupr.)Kuzen]的木屑为原料提取阿拉伯半乳聚糖(AG、松针多糖),然后用超声法对所提取的阿拉伯半乳聚糖进行降解。以降解松针多糖对自由基清除率为检测指标,通过单因素试验考察超声功率、降解温度、降解时间、料液比对松针多糖体外抗氧化活性的影响。再利用正交试验确定的最佳降解条件为:降解温度70℃、降解时间2.0h、料液比1∶250、功率150W。在抗氧化活性方面,松针多糖质量浓度越高,对·OH清除率越高,且降解后比降解前在清除·OH和DPPH上有一定提高。
Arabinogalactan(AG,or larch polysaccharide)was extracted from the sawdust of larch [Larix gmelinii(Rupr.)Kuzen].Then,the obtained larch polysaccharide was physically degraded by ultrasound-assistance.The free radical scavenging ability of decomposed larch polysaccharide was selected as an indicator on the evaluation of the antioxidant activity of larch polysaccharide.Single-factor tests were carried out in the study including ultrasonic power,degradation temperature,degradation time,and liquid-to-solid ratio.The optimum degradation condition was acquired with the analysis of the orthogonal tests as following:the degradation temperature was 70℃;the degradation time was 2.0 h;the liquid-to-solid ratio was 1∶250;the power was 150 W.The results of antioxidant activity showed that the scavenging rate of the sample to·OH would increase with the increase of the mass concentration of the polysaccharide.Further,the degraded arabinogalactan will enhance the ability of scavenging ·OH and DPPH compared with the original sample in the field of antioxidant activity.
Arabinogalactan(AG,or larch polysaccharide)was extracted from the sawdust of larch [Larix gmelinii(Rupr.)Kuzen].Then,the obtained larch polysaccharide was physically degraded by ultrasound-assistance.The free radical scavenging ability of decomposed larch polysaccharide was selected as an indicator on the evaluation of the antioxidant activity of larch polysaccharide.Single-factor tests were carried out in the study including ultrasonic power,degradation temperature,degradation time,and liquid-to-solid ratio.The optimum degradation condition was acquired with the analysis of the orthogonal tests as following:the degradation temperature was 70℃;the degradation time was 2.0 h;the liquid-to-solid ratio was 1∶250;the power was 150 W.The results of antioxidant activity showed that the scavenging rate of the sample to·OH would increase with the increase of the mass concentration of the polysaccharide.Further,the degraded arabinogalactan will enhance the ability of scavenging ·OH and DPPH compared with the original sample in the field of antioxidant activity.
引文
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[18]任瑞,马海乐,刘斌,等.超声降解香菇多糖及其体外抗氧化活性的研究[J].安徽农业科学,Journal of Anhui Agri.Sci.2008,36(17):7061-7062,7065.
[19]龚淑俐,邓放明.真菌多糖的生物活性研究进展[J].食物与营养,2006(1):27-28.
[20]闵婷,孙杰,黄菲,等.超声处理对龙眼果肉多糖理化特征及免疫调节活性的影响[J].现代食品科技,2016,32(5):125-125.
[21]王小梅,薛慧君,孙润广,等.超声提取功率对麦冬多糖体外清除羟自由基作用影响的研究[J].食品工业科技,2011,32(04):73-74.
[2]Chandrasekaran R,Janaswamy S.Mophology of Western larch arabinogalactan[J].Carbohydrate Research,2002,337(21-23):2211-2222.
[3]任瑞,马海乐,刘斌,等.超声降解香菇多糖及其体外抗氧化活性的研究[J].安徽农业科学,2008,36(17):7061-7062,7065.
[4]高玉杰,吕海涛.酸法降解浒苔多糖及其清除羟自由基活性研究[J].食品科学,2013,34(16):62-66.
[5]魏涛,何培新,郑俊丽.铁棍山药水溶性多糖的超声波提取工艺及体外抗氧化活性的研究[J].河南工业大学学报(自然科学版),2010,31(6):25-28.
[6]阿吉姑·阿布都热西提,买尔旦·马合木提,马合木提·乌斯满,等.维生素C清除自由基能力3种检测方法的比较[J].新疆医科大学学报,2008,31(5):578-579,582.
[7]李坚斌,李琳,李冰,等.超声降解多糖研究进展[J].食品工业科技,2006,27(9):181-184.
[8]马艳丽,方桂珍,等.超声波法提取木材中阿拉伯半乳聚糖的工艺参数优化[J].林产化工通讯,2004,38(5):6-7.
[9]方桂珍,马艳丽,徐凤英.等超声波法提取木材中的阿拉伯半乳聚糖的研究[J].林产化学与工业,2004,24(4):104-106.
[10]Thompson L H,Doraiswamy L K.Sonochemistry:science and engineering[J].Industrial&Engineering Chemistry Research,1999,38(4):1215-1249.
[11]方桂珍,黄占华,李淑君.微波法提取木材中阿拉伯半乳聚糖[J].林产化学与工业,2003,23(3):81-84.
[12]冯若,赵逸云,李化茂.超声在生物技术中应用的研究进展[J].生物化学与生物物理进展,1994,21(6):500-503,562.
[13]DORAISWAMYLKTH.Science and engineering industrial&engineering chemistry research[J].Sonochemistry,1999,38(4):1215.
[14]苟劲.超声波在化学化工中的应用研究[J].重庆工学院学报,2002,16(3):76.
[16]GOELLNER E M,UTERMOEHLEN J,KRAMER R,et al.Structure of arabinogalactan from Larix laricina and its reactivitywith antibodies directed against type-Ⅱ-arabinogalactans[J].Carbohydrate Polymers,2011,86(4):1739-1744.
[17]黄桂东,钟先锋.阿拉伯半乳聚糖的研究进展[J].食品与机械,2006,22(4):141-144.
[18]任瑞,马海乐,刘斌,等.超声降解香菇多糖及其体外抗氧化活性的研究[J].安徽农业科学,Journal of Anhui Agri.Sci.2008,36(17):7061-7062,7065.
[19]龚淑俐,邓放明.真菌多糖的生物活性研究进展[J].食物与营养,2006(1):27-28.
[20]闵婷,孙杰,黄菲,等.超声处理对龙眼果肉多糖理化特征及免疫调节活性的影响[J].现代食品科技,2016,32(5):125-125.
[21]王小梅,薛慧君,孙润广,等.超声提取功率对麦冬多糖体外清除羟自由基作用影响的研究[J].食品工业科技,2011,32(04):73-74.