地蜂子中儿茶素含量测定及其醇浸物对软组织损伤的影响
|
摘要
目的:建立地蜂子中儿茶素含量测定方法,观察地蜂子醇浸物对软组织损伤小鼠的治疗效果。方法:采用薄层色谱法(TLC)对药材进行定性鉴别,用高效液相色谱法(HPLC)测定地蜂子中儿茶素的含量;采用砝码法制作30只小鼠软组织损伤模型,将模型小鼠均分为空白组(涂抹生理盐水)、阳性对照组(涂抹云南白药)及地蜂子组(涂抹地蜂子液),连续给药3 d,记录小鼠损伤症候指数、观察治疗效果。结果:TLC斑点清晰,重复性好;地蜂子中儿茶素线性范围为0.017～0.086 g/L,r=0.999 4,回收率(n=6)为100.13%(RSD=2.18);与空白组比较,给药后第3天地蜂子组小鼠的损伤症候指数显著降低(P<0.05或P<0.01),损伤局部消肿效果较好。结论:本研究建立的高效液相色谱(HPLC)测定方法简单、准确,可用于地蜂子中儿茶素含量的测定;地蜂子醇浸物对小鼠软组织损伤具有改善作用。
Objective: To establish stable determination method of catechin in Potentilla freyniana, and to observe the therapeutic effect of Potentilla freyniana on acute soft tissue injury. Method: TLC was adopted for qualitative identification of samples, and HPLC method was used to determine the content of catechin.The mice model of acute soft tissue injury were established by weight blow method, mouse were randomized into three groups and given different drugs. The mice were treated for 3 consecutive days, and the injury symptom score was observed and using SPSS17.0 software to compare the differences between groups for analysis of variance. Results: TLC spots of samples were clear and well repeatability. The linear range of catechin of Potentilla freyniana was 0.017～0.086 mg/mL, the correlation coefficient was 0.999 4,and the recovery rate was 100.13%(n=6,RSD=2.18). Compared with the blank group, the score of soft tissue injury in mice decreased significantly on the third day after treatment. Conclusion: The HPLC method established in this experiment is simple and accurate, and can be used for the determination of catechin in Potentilla freyniana and extract of Potentilla freyniana can diminish the soft tissue injury in mice.
Objective: To establish stable determination method of catechin in Potentilla freyniana, and to observe the therapeutic effect of Potentilla freyniana on acute soft tissue injury. Method: TLC was adopted for qualitative identification of samples, and HPLC method was used to determine the content of catechin.The mice model of acute soft tissue injury were established by weight blow method, mouse were randomized into three groups and given different drugs. The mice were treated for 3 consecutive days, and the injury symptom score was observed and using SPSS17.0 software to compare the differences between groups for analysis of variance. Results: TLC spots of samples were clear and well repeatability. The linear range of catechin of Potentilla freyniana was 0.017～0.086 mg/mL, the correlation coefficient was 0.999 4,and the recovery rate was 100.13%(n=6,RSD=2.18). Compared with the blank group, the score of soft tissue injury in mice decreased significantly on the third day after treatment. Conclusion: The HPLC method established in this experiment is simple and accurate, and can be used for the determination of catechin in Potentilla freyniana and extract of Potentilla freyniana can diminish the soft tissue injury in mice.
引文
[1] 湖北省食品药品监督管理局.湖北省中药材质量标准[S].湖北:湖北科学技术出版社,2009:50.
[2] 谢宗万.全国中草药汇编(上册)[M].2版.北京:人民卫生出版社,2000:352-353.
[3] 朱敏英.地蜂子在土家族医药中的使用及其镇痛作用的实验研究[J].时珍国医国药,2005,(8):748-749.
[4] 刘梁,韩定献,周军,等.三叶委陵菜根中三萜类化合物抗病毒作用研究[J].时珍国医国药,2006,(8):1484-1485.
[5] 许正刚,闵运江.安徽产委陵菜属7种药用种类的黄酮提取与分析[J].安徽农业科学,2010,38(6):2929-2933.
[6] 陈会玲,闫斌,覃祝,等.地蜂子化学成分的研究[J].中成药,2015,37(12):2674-2677.
[7] 曾亮,黄建安,李赤翎,等.儿茶素的抑菌效果及机理研究[J].食品工业科技,2009 (5):89-92.
[8] 周露露,高原,牛知慧,等.茶叶中儿茶素的药理作用及其研究进展[J].辽宁化工,2018,47(4):316-318.
[9] TANG L,LI L,YANG J,et al.Potential benefit of (-)-epigallocatechin-3-gallate for macrovascular complications in diabetes[J].Brazilian Journal of Medical & Biological Research,2017,50(10):121-124.
[10]王丽丽,王坤波,黄建安,等.茶叶中儿茶素薄层色谱分离所用展开剂的优化[J].湖南农业大学学报(自然科学版),2012,38(1):102-105.
[11]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:66.
[12]韩建伟,刘新国,王文琼.HPLC法测定三叶委陵菜药材中儿茶素含量研究[J].湖北中医学院学报,2008,(3):55-56.
[13]张琪,徐维玲,李翠芹.HPLC法同时测定茶叶中儿茶素类和咖啡因的含量[J].食品工业科技,2015,36(4):53-56.
[14]覃博,韦葛谨,潘小清.接活跌打膏对急性软组织损伤大鼠的影响及机制研究[J].中药药理与临床,2016,32(3):140-143.
[15]周国林,姚全胜,潘玉英.一种动物软组织损伤的实验方法[J].中国药理学通报,1991,(5):396-398.
[16]XIAO B,LI Q.Soft tissue contusion repairing effects of Hong Yao with different penetration enhancers[J].Journal of Ethnopharmacology,2013,148(3):610-616.
[17]陈会玲.地蜂子乙酸乙酯部位化学成分研究[D].武汉:湖北中医药大学,2015.
[18]陆雪丽,潘晓鹃,邓萌萌,等.鸡血藤提取物的TLC鉴别及儿茶素和表儿茶素的含量测定[J].中国实验方剂学杂志,2018,24(18):88-92.
[19]朱国军,区棋铭,李振球,等.高效液相色谱法同时测定柑普茶中橙皮苷和6种儿茶素[J].化学分析计量,2018,27(6):51-55.
[20]王衍全,杨豪.中医筋伤学[M].北京:人民军医出版社,2006:12-14.
[21]谢春莲,郑永周,李碧妍.近10 年急性软组织损伤实验动物模型建立方法的研究进展[J].光明中医,2013,28(4):854-855.
[22]覃祝.地蜂子体外抗HSV-1病毒活性及其作用机理的初步研究[D].武汉:湖北中医药大学,2016.
[23]陆丽,曹红九.地蜂子免疫作用的药理研究[J].中国继续医学教育,2018,10(36):112-114.
[24]朱春城,彭力平,谢增军.中药外敷治疗急性软组织损伤的动物实验研究进展[J].中国实验方剂学杂志,2011,17(14):298-301.
[2] 谢宗万.全国中草药汇编(上册)[M].2版.北京:人民卫生出版社,2000:352-353.
[3] 朱敏英.地蜂子在土家族医药中的使用及其镇痛作用的实验研究[J].时珍国医国药,2005,(8):748-749.
[4] 刘梁,韩定献,周军,等.三叶委陵菜根中三萜类化合物抗病毒作用研究[J].时珍国医国药,2006,(8):1484-1485.
[5] 许正刚,闵运江.安徽产委陵菜属7种药用种类的黄酮提取与分析[J].安徽农业科学,2010,38(6):2929-2933.
[6] 陈会玲,闫斌,覃祝,等.地蜂子化学成分的研究[J].中成药,2015,37(12):2674-2677.
[7] 曾亮,黄建安,李赤翎,等.儿茶素的抑菌效果及机理研究[J].食品工业科技,2009 (5):89-92.
[8] 周露露,高原,牛知慧,等.茶叶中儿茶素的药理作用及其研究进展[J].辽宁化工,2018,47(4):316-318.
[9] TANG L,LI L,YANG J,et al.Potential benefit of (-)-epigallocatechin-3-gallate for macrovascular complications in diabetes[J].Brazilian Journal of Medical & Biological Research,2017,50(10):121-124.
[10]王丽丽,王坤波,黄建安,等.茶叶中儿茶素薄层色谱分离所用展开剂的优化[J].湖南农业大学学报(自然科学版),2012,38(1):102-105.
[11]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:66.
[12]韩建伟,刘新国,王文琼.HPLC法测定三叶委陵菜药材中儿茶素含量研究[J].湖北中医学院学报,2008,(3):55-56.
[13]张琪,徐维玲,李翠芹.HPLC法同时测定茶叶中儿茶素类和咖啡因的含量[J].食品工业科技,2015,36(4):53-56.
[14]覃博,韦葛谨,潘小清.接活跌打膏对急性软组织损伤大鼠的影响及机制研究[J].中药药理与临床,2016,32(3):140-143.
[15]周国林,姚全胜,潘玉英.一种动物软组织损伤的实验方法[J].中国药理学通报,1991,(5):396-398.
[16]XIAO B,LI Q.Soft tissue contusion repairing effects of Hong Yao with different penetration enhancers[J].Journal of Ethnopharmacology,2013,148(3):610-616.
[17]陈会玲.地蜂子乙酸乙酯部位化学成分研究[D].武汉:湖北中医药大学,2015.
[18]陆雪丽,潘晓鹃,邓萌萌,等.鸡血藤提取物的TLC鉴别及儿茶素和表儿茶素的含量测定[J].中国实验方剂学杂志,2018,24(18):88-92.
[19]朱国军,区棋铭,李振球,等.高效液相色谱法同时测定柑普茶中橙皮苷和6种儿茶素[J].化学分析计量,2018,27(6):51-55.
[20]王衍全,杨豪.中医筋伤学[M].北京:人民军医出版社,2006:12-14.
[21]谢春莲,郑永周,李碧妍.近10 年急性软组织损伤实验动物模型建立方法的研究进展[J].光明中医,2013,28(4):854-855.
[22]覃祝.地蜂子体外抗HSV-1病毒活性及其作用机理的初步研究[D].武汉:湖北中医药大学,2016.
[23]陆丽,曹红九.地蜂子免疫作用的药理研究[J].中国继续医学教育,2018,10(36):112-114.
[24]朱春城,彭力平,谢增军.中药外敷治疗急性软组织损伤的动物实验研究进展[J].中国实验方剂学杂志,2011,17(14):298-301.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |