毛木耳子实体蛛网病的病害特征及致病菌Cladobotryum cubitense的生理特性和防控策略
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摘要
从2010年起,在江苏省丰县毛木耳Auriculariacornea产区观察到蛛网病症状,该病害的发生呈逐年上升趋势,对毛木耳产量和产业造成了严重的损失。经过2015–2016年两年的调查研究,分析毛木耳蛛网病的发病规律。致病菌分别与毛木耳菌丝体和子实体的互作研究表明,该病原真菌仅危害毛木耳子实体,使毛木耳子实体的子实层出现裂缝,进而引起子实体畸形甚至停止生长。该病原本被鉴定为Cladobotryum cubitense。经37℃高温处理7d可抑制其菌丝的生长,50%咪鲜胺锰盐也能够抑制C. cubitense菌丝的生长,为减轻或控制毛木耳蛛网病的发生和传播提供了可行性策略。
From 2010, the symptom of cob-web disease was found in Auricularia cornea cultivation area in Fengxian, Jiangsu Province. The ocurrence of this disease exhibited an increasing trend year by year, causing a heavy loss in A. cornea production. According to the two year survey from 2015 to 2016, the epidemical law of cob-web in A. cornea was analyzed. It was demonstrated that the pathogen only infected fruiting bodies, leading cracked fruiting bodies to deform and cease from growth. The pathogen was identified as Cladobotrum cubitense.The high temperature treatment at 37°C for 7 d caused suspension of mycelial growth. 50% prochloraz-manganese chloride complex could also inhibit the growth of the pathogen. These results provide useful data in controlling the ocurrence and spread of cob-web disease of A. cornea.
From 2010, the symptom of cob-web disease was found in Auricularia cornea cultivation area in Fengxian, Jiangsu Province. The ocurrence of this disease exhibited an increasing trend year by year, causing a heavy loss in A. cornea production. According to the two year survey from 2015 to 2016, the epidemical law of cob-web in A. cornea was analyzed. It was demonstrated that the pathogen only infected fruiting bodies, leading cracked fruiting bodies to deform and cease from growth. The pathogen was identified as Cladobotrum cubitense.The high temperature treatment at 37°C for 7 d caused suspension of mycelial growth. 50% prochloraz-manganese chloride complex could also inhibit the growth of the pathogen. These results provide useful data in controlling the ocurrence and spread of cob-web disease of A. cornea.
引文
Adie B,Grogan H,Archer S,Mills P,2006.Temporal and spatial dispersal of Cladobotryum conidia in the controlled environment of a mushroom growing room.Applied and Environmental Microbiology,72(11):7212-7217
Carrasco J,Navarro MJ,Gea FJ,2017.Cobweb,a serious pathology in mushroom crops:a review.Spanish Journal of Agricultural Research,15(2):19
Carrasco J,Navarro MJ,Santos M,Diánez F,Gea FJ,2016.Incidence,identification and pathogenicity of Cladobotryum mycophilum,causal agent of cobweb disease on Agaricus bisporus mushroom crops in Spain.Annals of Applied Biology,168(2):214-224
Chen Y,Xue Y,2018.Purification,chemical characterization and antioxidant activities of a novel polysaccharide from Auricularia cornea Ehrenb.International Journal of Biological Macromolecules,120:1087-1092
Desrumeaux B,2005.Cobweb disease:an overview.Pest and diseases.Mushroom Business,15:16-17
Eicker A,Peng JT,Chen ZC,1990.A pseudohansfordia-disease of sawdust-cultivated Auricularia mesenterica in Taiwan.Botanical Bulletin of Academia Sinica,31:205-210
Fang ZD,1998.Method of plant pathogen research.3rd ed.Agriculture Press,Beijing.10-11(in Chinese)
Fletcher JT,Gaze RH,2008.Mushroom pest and disease control:a color handbook.Manson Publishing Ltd.Academic Press,San Diego.35-62
Gahukar RT,2014.Mushroom pest and disease management using plant-derived products in the tropics:a review.International Journal of Vegetable Science,20(1):78-88
Gea FJ,Tello JC,Navarro MJ,2010.Efficacy and effects on yield of different fungicides for control of wet bubble disease of mushroom caused by the mycoparasite Mycogone perniciosa.Crop Protection,29(9):1021-1025
Grogan HM,2006.Fungicide control of mushroom cobweb disease caused by Cladobotryum strains with different benzimidazole resistance profiles.Pest Management Science,62(2):153-161
Kim MK,Seuk SW,Lee YH,Kim HR,Cho KM,2014.Fungicide sensitivity and characterization of cobweb disease on a Pleurotus eryngii mushroom crop caused by Cladobotryum mycophilum.The Plant Pathology Journal,30(1):82
McKay GJ,Egan D,Morris E,Scott C,Brown AE,1999.Genetic and morphological characterization of Cladobotryum species causing cobweb disease of mushrooms.Applied and Environmental Microbiology,65(2):606-610
P?ldmaa K,2011.Tropical species of Cladobotryum and Hypomyces producing red pigments.Studies in Mycology,68:1-34
P?ldmaa K,Samuels GJ,2004.Fungicolous hypocreaceae(Ascomycota:Hypocreales)from Khao Yai National Park,Thailand.Sydowia,56:79-130
Poto?nik I,Vukojevi?J,Staji?M,Rekanovi?E,Milija?evi?S,Todorovi?B,Stepanovi?M,2009.In vitro toxicity of selected fungicides from the groups of benzimidazoles and demethylation inhibitors to Cladobotryum dendroides and Agaricus bisporus.Journal of Environmental Science and Health Part B,44(4):365-370
Tamm H,P?ldmaa K,2013.Diversity,host associations,and phylogeography of temperate aurofusarin-producing Hypomyces/Cladobotryum,including causal agents of cobweb disease of cultivated mushrooms.Fungal Biology,117(5):348-367
Wang GZ,Guo MP,Bian YB,2015.First report of Cladobotryum protrusum causing cobweb disease on the edible mushroom Coprinus comatus.Plant Disease,99(2):287-287
Wang GZ,Ma CJ,Zhou SS,P?ldmaa K,Tamm H,Luo Y,Guo MP,Ma XL,Bian YB,Zhou Y,2018.First report of cobweb disease of Auricularia polytricha(Mont.)Sacc.caused by Cladobotryum cubitense in Xuzhou,China.Plant Disease,102(7):1453-1453
Wu NJ,Chiou FJ,Weng YM,Yu ZR,Wang BJ,2014.In vitro hypoglycemic effects of hot water extract from Auricularia polytricha(wood ear mushroom).International Journal of Food Sciences and Nutrition,65(4):502-506
Yu M,Xu X,Qing Y,Luo X,Yang Z,Zheng L,2009.Isolation of an anti-tumor polysaccharide from Auricularia polytricha(jew’s ear)and its effects on macrophage activation.European Food Research and Technology,228(3):477-485
方中达,1998.植病研究方法.第三版.北京:农业出版社.10-11
Carrasco J,Navarro MJ,Gea FJ,2017.Cobweb,a serious pathology in mushroom crops:a review.Spanish Journal of Agricultural Research,15(2):19
Carrasco J,Navarro MJ,Santos M,Diánez F,Gea FJ,2016.Incidence,identification and pathogenicity of Cladobotryum mycophilum,causal agent of cobweb disease on Agaricus bisporus mushroom crops in Spain.Annals of Applied Biology,168(2):214-224
Chen Y,Xue Y,2018.Purification,chemical characterization and antioxidant activities of a novel polysaccharide from Auricularia cornea Ehrenb.International Journal of Biological Macromolecules,120:1087-1092
Desrumeaux B,2005.Cobweb disease:an overview.Pest and diseases.Mushroom Business,15:16-17
Eicker A,Peng JT,Chen ZC,1990.A pseudohansfordia-disease of sawdust-cultivated Auricularia mesenterica in Taiwan.Botanical Bulletin of Academia Sinica,31:205-210
Fang ZD,1998.Method of plant pathogen research.3rd ed.Agriculture Press,Beijing.10-11(in Chinese)
Fletcher JT,Gaze RH,2008.Mushroom pest and disease control:a color handbook.Manson Publishing Ltd.Academic Press,San Diego.35-62
Gahukar RT,2014.Mushroom pest and disease management using plant-derived products in the tropics:a review.International Journal of Vegetable Science,20(1):78-88
Gea FJ,Tello JC,Navarro MJ,2010.Efficacy and effects on yield of different fungicides for control of wet bubble disease of mushroom caused by the mycoparasite Mycogone perniciosa.Crop Protection,29(9):1021-1025
Grogan HM,2006.Fungicide control of mushroom cobweb disease caused by Cladobotryum strains with different benzimidazole resistance profiles.Pest Management Science,62(2):153-161
Kim MK,Seuk SW,Lee YH,Kim HR,Cho KM,2014.Fungicide sensitivity and characterization of cobweb disease on a Pleurotus eryngii mushroom crop caused by Cladobotryum mycophilum.The Plant Pathology Journal,30(1):82
McKay GJ,Egan D,Morris E,Scott C,Brown AE,1999.Genetic and morphological characterization of Cladobotryum species causing cobweb disease of mushrooms.Applied and Environmental Microbiology,65(2):606-610
P?ldmaa K,2011.Tropical species of Cladobotryum and Hypomyces producing red pigments.Studies in Mycology,68:1-34
P?ldmaa K,Samuels GJ,2004.Fungicolous hypocreaceae(Ascomycota:Hypocreales)from Khao Yai National Park,Thailand.Sydowia,56:79-130
Poto?nik I,Vukojevi?J,Staji?M,Rekanovi?E,Milija?evi?S,Todorovi?B,Stepanovi?M,2009.In vitro toxicity of selected fungicides from the groups of benzimidazoles and demethylation inhibitors to Cladobotryum dendroides and Agaricus bisporus.Journal of Environmental Science and Health Part B,44(4):365-370
Tamm H,P?ldmaa K,2013.Diversity,host associations,and phylogeography of temperate aurofusarin-producing Hypomyces/Cladobotryum,including causal agents of cobweb disease of cultivated mushrooms.Fungal Biology,117(5):348-367
Wang GZ,Guo MP,Bian YB,2015.First report of Cladobotryum protrusum causing cobweb disease on the edible mushroom Coprinus comatus.Plant Disease,99(2):287-287
Wang GZ,Ma CJ,Zhou SS,P?ldmaa K,Tamm H,Luo Y,Guo MP,Ma XL,Bian YB,Zhou Y,2018.First report of cobweb disease of Auricularia polytricha(Mont.)Sacc.caused by Cladobotryum cubitense in Xuzhou,China.Plant Disease,102(7):1453-1453
Wu NJ,Chiou FJ,Weng YM,Yu ZR,Wang BJ,2014.In vitro hypoglycemic effects of hot water extract from Auricularia polytricha(wood ear mushroom).International Journal of Food Sciences and Nutrition,65(4):502-506
Yu M,Xu X,Qing Y,Luo X,Yang Z,Zheng L,2009.Isolation of an anti-tumor polysaccharide from Auricularia polytricha(jew’s ear)and its effects on macrophage activation.European Food Research and Technology,228(3):477-485
方中达,1998.植病研究方法.第三版.北京:农业出版社.10-11