基于MnO_2纳米线-还原石墨烯复合修饰电极的多巴胺电化学检测
|
摘要
通过电化学还原法制备MnO_2纳米线/还原石墨烯复合修饰电极(MnO_2-RGO/GCE),用于多巴胺(DA)的检测。采用扫描电镜和X-射线粉末衍射对不同的修饰电极微观形貌进行了表征,优化了电化学还原条件和测定DA实验条件。此外,还研究DA在裸电极及RGO或MnO_2-RGO修饰电极上的循环伏安响应。MnO_2-RGO/GCE复合修饰电极实现AA、DA和UA氧化峰的有效分离,AA-DA和DA-UA的氧化峰电位差分别为268和128 m V。检测DA的线性范围为0.06~1.0μmol/L和1.0~80μmol/L,检出限为1.0 nmol/L(S/N=3)。制备的MnO_2-RGO/GCE成功用于人血清样品的多巴胺含量分析。
MnO_2 nanowires-reduced graphene oxide composite( MnO_2-RGO) was used to modify glassy carbon electrodes( GCE) and applied for the electrochemical determination of dopamine( DA). The microstructure of MnO_2 nanowires and MnO_2-RGO nanocomposite material was characterized by scanning microscope and X-ray powder diffraction. Then the electrochemical reduction condition for preparing MnO_2-RGO/GCE and experimental conditions for determining DA were optimized systematically. The electrochemical behavior of DA on the bare electrode and RGO or MnO_2-RGO modified electrodes was also investigated in p H3.5 phosphate buffer solution( PBS) by cyclic voltammetry. The results shows that the oxidation peaks of ascorbic acid( AA),dopamine( DA) and uric acid( UA) can be well separated and the peak to peak separations were 268 m V( AA-DA) and 128 m V( DA-UA),respectively. Moreover,the linear response ranges for the determination of DA were 0. 06-1. 0 μmol/L and 1. 0-80 μmol/L with the detection limit of1.0 nmol/L( S/N = 3). The proposed method has been applied to the determination of dopamine in human blood serum sample with satisfactory results.
MnO_2 nanowires-reduced graphene oxide composite( MnO_2-RGO) was used to modify glassy carbon electrodes( GCE) and applied for the electrochemical determination of dopamine( DA). The microstructure of MnO_2 nanowires and MnO_2-RGO nanocomposite material was characterized by scanning microscope and X-ray powder diffraction. Then the electrochemical reduction condition for preparing MnO_2-RGO/GCE and experimental conditions for determining DA were optimized systematically. The electrochemical behavior of DA on the bare electrode and RGO or MnO_2-RGO modified electrodes was also investigated in p H3.5 phosphate buffer solution( PBS) by cyclic voltammetry. The results shows that the oxidation peaks of ascorbic acid( AA),dopamine( DA) and uric acid( UA) can be well separated and the peak to peak separations were 268 m V( AA-DA) and 128 m V( DA-UA),respectively. Moreover,the linear response ranges for the determination of DA were 0. 06-1. 0 μmol/L and 1. 0-80 μmol/L with the detection limit of1.0 nmol/L( S/N = 3). The proposed method has been applied to the determination of dopamine in human blood serum sample with satisfactory results.
引文
1 Li L L,Liu H Y,Shen Y Y,Zhang J R,Zhu J J.Anal.Chem.,2011,83(3):661-665
2 Carrera V,Sabater E,Vilanova E,Sogorb M A.J.Chromatogr.B,2007,847:88-94
3 Guan C L,Ouyang J,Li Q L,Liu B H,Baeyens W R G.Talanta,2000,50:1197-1203
4 Wu H P,Cheng T L,Tseng W L.Langmuir,2007,23:7880-7885
5 Wang H Y,Sun Y,Tang B.Talanta,2002,57:899-907
6 Chen L F,Lu L L,Mo Y,Xu Z M,Xie S P,Yuan H Y,Xiao D,Choi M M F.Talanta,2011,85:56-62
7 Tian X Q,Cheng C M,Yuan H Y,Du J,Xiao D,Xie S P,Choi M M F.Talanta,2012,93:79-85
8 Zhang Y,Lin L L,Feng Z F,Zhou J Z,Lin Z H.Electrochim.Acta,2009,55:265-270
9 LI Chong,JIA Li-Ping,MA Rong-Na,JIA Wen-Li.Chem.J.Chinese Universities,2015,36(7):1282-1290李冲,贾丽萍,马荣娜,贾文丽.高等学校化学学报,2015,36(7):1282-1290
10 ZHANG Lei,LIN Xiang-Qin.Chem.J.Chinese Universities,2003,24(4):591-594张雷,林祥钦.高等学校化学学报,2003,24(4):591-594
11 CHEN Dan,CAO Zhong,LIU Feng,WU Ling,XUN Yan,HE Jing-Lin,XIAO Zhong-Liang.Chinese J.Anal.Chem.,2016,44(10):1593-1599陈丹,曹忠,刘峰,吴玲,寻艳,何婧琳,肖忠良.分析化学,2016,44(10):1593-1599
12 Ulubay S,Dursun Z.Talanta,2010,80(3):1461-1466
13 Bi H Q,Li Y H,Liu S F,Guo P Z,Wei Z B,Lv C X,Zhang J Z,Zhao X S.Sens.Actuators B,2012,171:1132-1140
14 Yang S L,Li G,Yin Y L,Li JJ,Qu L B.J.Electroanal.Chem.,2013,703:45-51
15 Liu M L,Chen Q,Lai C L,Zhang Y Y,Deng J H,Li H T,Yao S Z.Biosens.Bioelectron.,2013,48:75-81
16 Lian Q W,He Z F,He Q,Luo A,Yan K W,Zhang D X,Lu X Q,Zhou X B.Anal.Chim.Acta,2014,823:32-39
17 Wu D,Li YY,Zhang Y,Wang P P,Wei Q,Du B.Electrochim.Acta,2014,116:244-249
18 Débart A,Paterson A J,Bao J L,Bruce P G.Angew.Chem.Int.Edit.,2008,47:4521-4524
19 Jiao F,Bruce P G.Adv.Mater.,2007,19:657-660
20 Cheng F Y,Su Y,Liang J,Tao Z L,Chen J.Chem.Mater.,2010,22:898-905
21 Gong K P,Yu P,Su L,Xiong S X,L.Q.Mao L Q.J.Phys.Chem.,2007,111:1882-1887
22 Liu R,Duay J,Lee S B.ACS Nano,2010,4:4299-4307
23 Xu M W,Jia W,Bao S J,Su Z,Dong B.Electrochim.Acta,2010,55:5117-5122
24 Li Y L,Zhang J,Zhu H,Yang F,Yang X R.Electrochim.Acta,2010,55:5123-5128
25 Xu B,Ye M L,Yu Y X,Zhang W D.Anal.Chim.Acta,2010,674:20-26
26 Cao X,Wang N,Guo L.Sens.Actuators B,2009,137:710-714
27 Xu J J,Zhao W,Luo X L,Chen H Y.Chem.Commun.,2005,6(6):792-794
28 Bai Y H,Xu J J,Chen H Y.Biosens.Bioelectron.,2009,24:2985-2990
29 Wang Z H,Tang J,Zhang F F,Xia J F,Shi G Y,Xia Y Z,Xia L H,Qin L C.Int.J.Electrochem.Sci.,2013,8(7):9967-9976
30 Rani K K,Devasenathipathy R,Wang S F,Yang C.Ionics,2017,(7-8):1-8
31 Zhang L C,Liu Z H,Lv H,Tang X H,Ooi K.J.Phys.Chem.C,2007,111(24):8418-8423
32 Wang M F,Wang C,Wang G F,Zhang W,Bin F.Electroanalysis,2010,22(10):1123-1129
33 Deng P H,Xu Z F,Zeng R Y,Ding C X,Food Chem.,2015,180:156-163
34 Deng P H,Xu Z F,Kuang Y F.J.Electroanal.Chem.,2013,707:7-14
35 Deng P H,Xu Z F,Feng Y L.Mater.Sci.Eng.C,2014,35:54-60
36 Deng P H,Xu Z F,Li J H.Microchim.Acta,2014,181:1077-1084
37 Hummers W S,Offeman R E.J.Am.Chem.Soc.,1958,80:1339-1340
38 Laviron E J.Electroanal.Chem.,1979,101(1):19-28
39 YE Bao-Xian,LI Feng-Ju,ZHANG Jun,JIN Bao-Hui.Chem.Res.,2003,14(1):44-46冶保献,李风菊,张俊,靳保辉.化学研究,2003,14(1):44-46
40 Huang Y,Cheng C,Tian X,Zheng B,Li Y,Yuan H,Xiao D,Choi M M F.Electrochim.Acta,2013,89:832-839
41 Reddy S,Swamy B E K,Chandrashekar B N,Chitravathi S,Jayadevappa H.Anal.Bioanal.Electrochem.,2012,4:186-196
42 Zhang F,Li Y,Gu Y E,Wang Z,Wang C.Microchim.Acta,2011,173(1-2):103-109
43 Reddy S,Swamy B E K,Jayadevappa H.Electrochim.Acta,2012,61:78-86
44 Gao W,Ye S,Shao M.J.Phys.Chem.Solids,2011,72(9):1027-1031
45 Adekunle A S,Agboola B O,Pillay J,Ozoemena K I.Sens.Actuators B,2010,148(1):93-102
2 Carrera V,Sabater E,Vilanova E,Sogorb M A.J.Chromatogr.B,2007,847:88-94
3 Guan C L,Ouyang J,Li Q L,Liu B H,Baeyens W R G.Talanta,2000,50:1197-1203
4 Wu H P,Cheng T L,Tseng W L.Langmuir,2007,23:7880-7885
5 Wang H Y,Sun Y,Tang B.Talanta,2002,57:899-907
6 Chen L F,Lu L L,Mo Y,Xu Z M,Xie S P,Yuan H Y,Xiao D,Choi M M F.Talanta,2011,85:56-62
7 Tian X Q,Cheng C M,Yuan H Y,Du J,Xiao D,Xie S P,Choi M M F.Talanta,2012,93:79-85
8 Zhang Y,Lin L L,Feng Z F,Zhou J Z,Lin Z H.Electrochim.Acta,2009,55:265-270
9 LI Chong,JIA Li-Ping,MA Rong-Na,JIA Wen-Li.Chem.J.Chinese Universities,2015,36(7):1282-1290李冲,贾丽萍,马荣娜,贾文丽.高等学校化学学报,2015,36(7):1282-1290
10 ZHANG Lei,LIN Xiang-Qin.Chem.J.Chinese Universities,2003,24(4):591-594张雷,林祥钦.高等学校化学学报,2003,24(4):591-594
11 CHEN Dan,CAO Zhong,LIU Feng,WU Ling,XUN Yan,HE Jing-Lin,XIAO Zhong-Liang.Chinese J.Anal.Chem.,2016,44(10):1593-1599陈丹,曹忠,刘峰,吴玲,寻艳,何婧琳,肖忠良.分析化学,2016,44(10):1593-1599
12 Ulubay S,Dursun Z.Talanta,2010,80(3):1461-1466
13 Bi H Q,Li Y H,Liu S F,Guo P Z,Wei Z B,Lv C X,Zhang J Z,Zhao X S.Sens.Actuators B,2012,171:1132-1140
14 Yang S L,Li G,Yin Y L,Li JJ,Qu L B.J.Electroanal.Chem.,2013,703:45-51
15 Liu M L,Chen Q,Lai C L,Zhang Y Y,Deng J H,Li H T,Yao S Z.Biosens.Bioelectron.,2013,48:75-81
16 Lian Q W,He Z F,He Q,Luo A,Yan K W,Zhang D X,Lu X Q,Zhou X B.Anal.Chim.Acta,2014,823:32-39
17 Wu D,Li YY,Zhang Y,Wang P P,Wei Q,Du B.Electrochim.Acta,2014,116:244-249
18 Débart A,Paterson A J,Bao J L,Bruce P G.Angew.Chem.Int.Edit.,2008,47:4521-4524
19 Jiao F,Bruce P G.Adv.Mater.,2007,19:657-660
20 Cheng F Y,Su Y,Liang J,Tao Z L,Chen J.Chem.Mater.,2010,22:898-905
21 Gong K P,Yu P,Su L,Xiong S X,L.Q.Mao L Q.J.Phys.Chem.,2007,111:1882-1887
22 Liu R,Duay J,Lee S B.ACS Nano,2010,4:4299-4307
23 Xu M W,Jia W,Bao S J,Su Z,Dong B.Electrochim.Acta,2010,55:5117-5122
24 Li Y L,Zhang J,Zhu H,Yang F,Yang X R.Electrochim.Acta,2010,55:5123-5128
25 Xu B,Ye M L,Yu Y X,Zhang W D.Anal.Chim.Acta,2010,674:20-26
26 Cao X,Wang N,Guo L.Sens.Actuators B,2009,137:710-714
27 Xu J J,Zhao W,Luo X L,Chen H Y.Chem.Commun.,2005,6(6):792-794
28 Bai Y H,Xu J J,Chen H Y.Biosens.Bioelectron.,2009,24:2985-2990
29 Wang Z H,Tang J,Zhang F F,Xia J F,Shi G Y,Xia Y Z,Xia L H,Qin L C.Int.J.Electrochem.Sci.,2013,8(7):9967-9976
30 Rani K K,Devasenathipathy R,Wang S F,Yang C.Ionics,2017,(7-8):1-8
31 Zhang L C,Liu Z H,Lv H,Tang X H,Ooi K.J.Phys.Chem.C,2007,111(24):8418-8423
32 Wang M F,Wang C,Wang G F,Zhang W,Bin F.Electroanalysis,2010,22(10):1123-1129
33 Deng P H,Xu Z F,Zeng R Y,Ding C X,Food Chem.,2015,180:156-163
34 Deng P H,Xu Z F,Kuang Y F.J.Electroanal.Chem.,2013,707:7-14
35 Deng P H,Xu Z F,Feng Y L.Mater.Sci.Eng.C,2014,35:54-60
36 Deng P H,Xu Z F,Li J H.Microchim.Acta,2014,181:1077-1084
37 Hummers W S,Offeman R E.J.Am.Chem.Soc.,1958,80:1339-1340
38 Laviron E J.Electroanal.Chem.,1979,101(1):19-28
39 YE Bao-Xian,LI Feng-Ju,ZHANG Jun,JIN Bao-Hui.Chem.Res.,2003,14(1):44-46冶保献,李风菊,张俊,靳保辉.化学研究,2003,14(1):44-46
40 Huang Y,Cheng C,Tian X,Zheng B,Li Y,Yuan H,Xiao D,Choi M M F.Electrochim.Acta,2013,89:832-839
41 Reddy S,Swamy B E K,Chandrashekar B N,Chitravathi S,Jayadevappa H.Anal.Bioanal.Electrochem.,2012,4:186-196
42 Zhang F,Li Y,Gu Y E,Wang Z,Wang C.Microchim.Acta,2011,173(1-2):103-109
43 Reddy S,Swamy B E K,Jayadevappa H.Electrochim.Acta,2012,61:78-86
44 Gao W,Ye S,Shao M.J.Phys.Chem.Solids,2011,72(9):1027-1031
45 Adekunle A S,Agboola B O,Pillay J,Ozoemena K I.Sens.Actuators B,2010,148(1):93-102
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |