Zn(Ⅱ)、硫化物和苯酚对短程硝化污泥活性的抑制
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摘要
重金属锌(Zn~(2+))、硫化物和苯酚是工业废水中常见的、困扰许多工业废水生物处理系统运行效果的污染物。采用批次实验分别研究了Zn~(2+)、硫化物和苯酚对短程硝化污泥的单一毒性以及抑制解除后的活性恢复情况,根据单因素实验结果选取实验水平,结合中心组合设计原理,采用2因素3水平的响应面分析法,考察了Zn~(2+)和苯酚以及硫化物和苯酚对短程硝化污泥活性的联合毒性抑制。Zn~(2+)、硫化物和苯酚的半抑制浓度IC50分别为38.29、139.13和3.46 mg·L~(-1),高浓度抑制剂实验组抑制解除后8 h,三者的短程硝化污泥活性恢复程度分别为7.46%、46.09%、22.69%。实验所得Zn~(2+)和苯酚以及硫化物和苯酚对短程硝化污泥活性的联合毒性抑制的二元二次响应面模型的多元相关系数R2分别为0.997 2和0.990 8,实验值与模型预测值吻合较好,可为短程硝化-厌氧氨氧化工艺处理实际废水提供理论指导。
Zinc(Zn~(2+)),sulfide and phenol,frequently detected in industrial wastewater,affect efficiencies of many biological industrial wastewater treatment systems.This study investigated the inhibition effects and recovery performance of Zn~(2+) ,sulfide and phenol on partial nitritation sludge by batch experiments.To investigate the combined suppression of Zn~(2+) and phenol,as well as sulfides and phenol on partial nitritation sludge,the two factors and three levels of response surface methodology was used based on the center combination design.The results showed that the half maximal inhibitory concentration (IC50) of Zn~(2+) ,sulfide and phenol on partial nitritation sludge were 38.29,139.13 and 3.46 mg·L~(-1),respectively.The recovery percentage of the activity of partial nitritation sludge after removing the three inhibitors within 8 hours were 7.46%,46.09% and 22.69%,respectively.The multiple correlation coefficient of the two binary quadratic response surface regression equations were 0.997 2 (Zn~(2+) and phenol) and 0.990 8 (sulfides and phenol),respectively.The experimental results are in good agreement with the predicted values of the model.It is shown that the model can provide theoretical guidance for the application of partial nitritation-anammox to treat wastewater.
Zinc(Zn~(2+)),sulfide and phenol,frequently detected in industrial wastewater,affect efficiencies of many biological industrial wastewater treatment systems.This study investigated the inhibition effects and recovery performance of Zn~(2+) ,sulfide and phenol on partial nitritation sludge by batch experiments.To investigate the combined suppression of Zn~(2+) and phenol,as well as sulfides and phenol on partial nitritation sludge,the two factors and three levels of response surface methodology was used based on the center combination design.The results showed that the half maximal inhibitory concentration (IC50) of Zn~(2+) ,sulfide and phenol on partial nitritation sludge were 38.29,139.13 and 3.46 mg·L~(-1),respectively.The recovery percentage of the activity of partial nitritation sludge after removing the three inhibitors within 8 hours were 7.46%,46.09% and 22.69%,respectively.The multiple correlation coefficient of the two binary quadratic response surface regression equations were 0.997 2 (Zn~(2+) and phenol) and 0.990 8 (sulfides and phenol),respectively.The experimental results are in good agreement with the predicted values of the model.It is shown that the model can provide theoretical guidance for the application of partial nitritation-anammox to treat wastewater.
引文
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[21]李娟英,赵庆祥,江敏.苯酚及其衍生物对氨氮生物硝化的抑制研究[J].环境工程学报,2008,2(1):27-30
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[2]王亚宜,黎力,马骁,等.厌氧氨氧化菌的生物特性及CANON厌氧氨氧化工艺[J].环境科学学报,2014,34(6):1362-1374
[3]RONGSAYAMANONT C,LIMPIYAKORN T,KHAN E.Effects of inoculum type and bulk dissolved oxygen concentration on achieving partial nitrification by entrapped-cell-based reactors[J].Bioresource Technology,2014,164:254-263
[4]邓征宇,杨春平,曾光明,等.Fenton-水解酸化-接触氧化工艺处理含苯酚制药废水[J].工业水处理,2010,30(7):68-71
[5]任源,韦朝海,吴超飞,等.焦化废水水质组成及其环境学与生物学特性分析[J].环境科学学报,2007,27(7):1094-1100
[6]高太忠,余国山,杨柳,等.垃圾渗滤液中溶解性有机物对土壤中重金属迁移的影响[J].环境工程学报,2011,5(5):1176-1180
[7]GUO Q,SHI Z J,XU J L,et al.Inhibition of the partial nitritation by roxithromycin and Cu(Ⅱ)[J].Bioresource Technology,2016,214:253-258
[8]李亚峰,张驰,王健.重金属离子对亚硝化的影响研究[J].水处理技术,2016,42(2):96-99
[9]WU S,BHATTACHARJEE A S,WEISSBRODT D G,et al.Effect of short term external perturbations on bacterial ecology and activities in a partial nitritation and anammox reactor[J].Bioresource Technology,2016,219:527-535
[10]路聪聪,葛士建,王淑莹,等.苯酚对氨氧化菌硝化和污泥性能冲击影响[J].化工学报,2012,63(8):2576-2583
[11]魏翔,任洪强,袁粒,等.混合重金属对硝化颗粒污泥毒性作用的析因实验研究[J].环境工程学报,2007,1(5):101-104
[12]ZHAO J,ZUO J,WANG X,et al.Geo Chip-based analysis of microbial community of a combined nitritation-anammox reactor treating anaerobic digestion supernatant[J].Water Research,2014,67:345-354
[13]GRAAF A V D A,BRUIJN D P,ROBERTSON L A,et al.Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J].Microbiology,1996,142(8):2187-2196
[14]国家环境保护总局.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002
[15]JIN R C,YANG G F,ZHANG Q Q,et al.The effect of sulfide inhibition on the ANAMMOX process[J].Water Research,2013,47(3):1459-1469
[16]CHENG M H,MINEAR R A.Heavy metals uptake by activated sludge[J].Journal of Water Pollution Control Federation,1975,47(2):362-376
[17]SEARS K,ALLEMAN J E,BARNARD J L,et al.Impacts of reduced sulfur components on active and resting ammonia oxidizers[J].Journal of Industrial Microbiology&Biotechnology,2004,31(8):369-378
[18]DENYER S P,STEWART G S A B.Mechanism of action of disinfectants[J].International Biodeterioration&Biodegradation,1998,41(3):261-268
[19]CECEN F,SEMERCI N,GEYIK A G.Inhibitory effects of Cu,Zn,Ni and Co on nitrification and relevance of speciation[J].Journal of Chemical Technology&Biotechnology,2010,85(4):520-528
[20]蒋玲燕,周振,王英俊,等.硫化物对污水处理厂硝化菌活性的抑制作用[J].环境工程学报,2012,6(11):4065-4068
[21]李娟英,赵庆祥,江敏.苯酚及其衍生物对氨氮生物硝化的抑制研究[J].环境工程学报,2008,2(1):27-30
[22]MURALIDHAR R V,CHIRUMAMILA R R,MARCHANT R,et al.A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources[J].Biochemical Engineering Journal,2001,9(1):17-23