用响应面法对鱼油纳米脂质体处方的优化
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摘要
利用响应面法对薄膜分散法制备鱼油纳米脂质体的处方进行优化,初步测定了其理化性质。并研究了用南极磷虾卵磷脂制备鱼油纳米脂质体的影响因素,主要包括磷脂浓度、胆固醇含量、吐温-80含量和鱼油含量,并通过响应面法优化制备工艺。最终得到的最佳处方为:磷脂浓度25mg/mL,m(磷脂)∶m(胆固醇)=2.5∶1,m(磷脂)∶m(鱼油)=6.5∶1,吐温-80含量为17%。此时鱼油纳米脂质体的粒径为21.20nm,包封率为88.24%,Zeta电位为-8.34mV,接近于模型预测值。
Response surface methodology(RSM)was selected to optimize the fish oil nanoliposomes prepared by thin film hydration(TFH)method.The initial physical and chemical properties of nanoliposomes were determined.The factors that influence the preparation of fish oil nanoliposomes with the phosphatidylcholine from Antarctic krill were studied,which included the content of phosphatidylcholine,cholesterol,Tween-80 and fish oil.The preparation was optimized through the RSM.The optimal prescription was obtained as follows:phosphatidylcholine content was 25 mg/mL,the ratio of phosphatidylcholine and cholesterol was 2.5∶1,the ratio of phosphatidylcholine and fish oil was 6.5∶1,the content of Tween-80 was 17%.Under these conditions,the mean diameter and polydispersity index of fish oil nanoliposomes was 21.20 nm,the encapsulation efficiency(EE)was 88.24%,Zeta potential was-8.34 mV,which was well matched with the predictive preparation.
Response surface methodology(RSM)was selected to optimize the fish oil nanoliposomes prepared by thin film hydration(TFH)method.The initial physical and chemical properties of nanoliposomes were determined.The factors that influence the preparation of fish oil nanoliposomes with the phosphatidylcholine from Antarctic krill were studied,which included the content of phosphatidylcholine,cholesterol,Tween-80 and fish oil.The preparation was optimized through the RSM.The optimal prescription was obtained as follows:phosphatidylcholine content was 25 mg/mL,the ratio of phosphatidylcholine and cholesterol was 2.5∶1,the ratio of phosphatidylcholine and fish oil was 6.5∶1,the content of Tween-80 was 17%.Under these conditions,the mean diameter and polydispersity index of fish oil nanoliposomes was 21.20 nm,the encapsulation efficiency(EE)was 88.24%,Zeta potential was-8.34 mV,which was well matched with the predictive preparation.
引文
[1]孙聚魁,邓英杰,曹金娜.纳米脂质体研究进展[J].沈阳药科大学学报,2010,27(12)∶993-997.
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[7]Dyerberg J,Bang H O,Stoffersen E,et al.Eicosapentaenoic Acid and Prevention of Thrombosis and Atherosclerosis[J].Lancet,1978,312(8081):117-119.
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[9]安树,刘宁.DHA脂质体的制备及其性质研究[J].食品工业科技,2010,1:99-102.
[10]D'Ascenzo R,D'Egidio S,Angelini L,et al.Parenteral Nutrition of Preterm Infants with a Lipid Emulsion Containing 10%Fish Oil:Effect on Plasma Lipids and Long-Chain Polyunsaturated Fatty Acids[J].J Pediatr,2011,159(1):33-38.
[11]赵静.南极磷虾磷脂提取纯化及抗衰老实验研究[D].青岛:中国海洋大学,2012.
[12]张潇予.南极磷虾磷脂酰胆碱的提纯[D].济南:山东师范大学,2012.
[13]张朋.鱼油的提取及其纳米脂质体的制备和性质研究[D].南昌:南昌大学,2013.
[14]Bangham A D,Standish M M,Watkins J C.Diffusion of univalent ions across the lamellae of swollen phospholipids[J].J Mol Biol,1965,13(1):238-252.
[2]施显赫,陈萌,高萌,等.响应面法优化脂质体包埋辣椒红色素的研究[J].食品工业科技,2012,33(11):235-237.
[3]唐仕,李嘉诚,张学良,等.联苯菊酯纳米乳的制备与表征[J].安徽农业科学,2011,11:6453-6455.
[4]阴婷婷.叶酸纳米脂质体的制备及性质研究[D].南昌:南昌大学,2011.
[5]公雯,朱家壁.纳米乳的优化方法与不稳定机制的研究进展[J].中国新药杂志,2010,12:1036-1040.
[6]薛长湖,陈修白,李兆杰,等.从鳀鱼油中提取高不饱和脂肪酸(EPA和DHA)的研究[J].中国水产科学,1994,1:55-60.
[7]Dyerberg J,Bang H O,Stoffersen E,et al.Eicosapentaenoic Acid and Prevention of Thrombosis and Atherosclerosis[J].Lancet,1978,312(8081):117-119.
[8]张静姝.深海鱼油辅助改善记忆功能的研究[D].天津:天津医科大学,2011.
[9]安树,刘宁.DHA脂质体的制备及其性质研究[J].食品工业科技,2010,1:99-102.
[10]D'Ascenzo R,D'Egidio S,Angelini L,et al.Parenteral Nutrition of Preterm Infants with a Lipid Emulsion Containing 10%Fish Oil:Effect on Plasma Lipids and Long-Chain Polyunsaturated Fatty Acids[J].J Pediatr,2011,159(1):33-38.
[11]赵静.南极磷虾磷脂提取纯化及抗衰老实验研究[D].青岛:中国海洋大学,2012.
[12]张潇予.南极磷虾磷脂酰胆碱的提纯[D].济南:山东师范大学,2012.
[13]张朋.鱼油的提取及其纳米脂质体的制备和性质研究[D].南昌:南昌大学,2013.
[14]Bangham A D,Standish M M,Watkins J C.Diffusion of univalent ions across the lamellae of swollen phospholipids[J].J Mol Biol,1965,13(1):238-252.