缩宫素生产工艺条件优化研究
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摘要
以自制的保护型缩宫素MBHA树脂为原料,通过优化裂解反应中的裂解液配比、反应时间并在低反应浓度下采用双氧水氧化进行成环反应,建立了缩宫素新生产工艺。新生产工艺操作简便、省时高效,且所得中间产物的含量提高、杂质减少,对缩宫素粗品纯化有利。
Using self-made protective oxytocin MBHA resin as a raw material, a new process for the production of oxytocin was established by optimizing the ratio of lysate in cleavage solution, reaction time and cyclization reaction by oxidation of hydrogen peroxide at low reaction concentration. This process was simple, time-saving and efficient, and the content of intermediate products was increased and the impurities were reduced, which was of benefit to the purification of crude oxytocin.
Using self-made protective oxytocin MBHA resin as a raw material, a new process for the production of oxytocin was established by optimizing the ratio of lysate in cleavage solution, reaction time and cyclization reaction by oxidation of hydrogen peroxide at low reaction concentration. This process was simple, time-saving and efficient, and the content of intermediate products was increased and the impurities were reduced, which was of benefit to the purification of crude oxytocin.
引文
[1]徐仲,李宁,刘卓.奥曲肽固相合成及环化的研究[J].哈尔滨工业大学学报, 2008, 40(2):292-295.
[2]王良友,潘和平,陈正英.多肽合成中几种形成二硫键方法的介绍[J].有机化学, 1998, 18(6):576-580.
[3]唐艳春,田桂玲,叶蕴华.环肽合成方法的研究进展[J].高等学校化学学报, 2000, 21(7):1056-1063.
[4]周艳荣,戴秋云.肽合成中多对二硫键的形成策略及分析方法[J].生物技术通讯, 2002, 13(3):232-233; 241.
[5]白泉,葛小娟,耿信笃.反相液相色谱对多肽的分离、纯化与制备[J].分析化学, 2002, 30(9):1126-1129.
[2]王良友,潘和平,陈正英.多肽合成中几种形成二硫键方法的介绍[J].有机化学, 1998, 18(6):576-580.
[3]唐艳春,田桂玲,叶蕴华.环肽合成方法的研究进展[J].高等学校化学学报, 2000, 21(7):1056-1063.
[4]周艳荣,戴秋云.肽合成中多对二硫键的形成策略及分析方法[J].生物技术通讯, 2002, 13(3):232-233; 241.
[5]白泉,葛小娟,耿信笃.反相液相色谱对多肽的分离、纯化与制备[J].分析化学, 2002, 30(9):1126-1129.