地木耳多糖的抗氧化活性与抗菌活性研究
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摘要
目的研究地木耳多糖的抗氧化性与抑菌活性。方法利用超声波提取法从地木耳中提取多糖,经初步纯化后,对多糖的抗氧化性和抑菌作用进行分析。结果地木耳多糖具有一定的抗氧化能力,且其抗氧化能力在一定的浓度范围内呈现良好的量效关系。地木耳多糖对·OH的清除率最高,最高可达70.53%,对DPPH的清除率次之,最高可达67.72%,对O_2-的清除能力最低,最高可达32.31%。地木耳多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、啤酒酵母均有一定的抑制作用,其中对大肠杆菌的抑菌效果最好,最小抑菌质量浓度为31.25μg/mL;对丹参枯萎病菌无抑制作用。结论地木耳多糖具有一定的抗氧化能力和抗菌能力。
Objective To research the antioxidant and antibacterial activity of polysaccharides from Nostoc commune. Methods Polysaccharide was extracted from Nostoc commune by ultrasonic extraction. After initial purification, the antioxidant and antibacterial effects of polysaccharides were analyzed. Results The polysaccharide from Nostoc commune had certain antioxidant capacity, and its antioxidant capacity showed a good dose-effect relationship within a certain concentration range. The polysaccharide had the highest clearance rate of ·OH, up to 70.53%, followed by DPPH, up to 67.72%, and the lowest removal rate of O_2-, up to 32.31%. The Nostoc communepolysaccharide had a certain inhibitory effect on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Saccharomyces cerevisiae, among which the antibacterial effect on E. coli was the best, the minimum inhibitory mass concentration was 31.25 μg/mL, and it had no inhibitory effect on Salvia miltiorrhiza wilt. ConclusionPolysaccharides from Nostoc Commune has certain anti-oxidation ability and antibacterial ability.
Objective To research the antioxidant and antibacterial activity of polysaccharides from Nostoc commune. Methods Polysaccharide was extracted from Nostoc commune by ultrasonic extraction. After initial purification, the antioxidant and antibacterial effects of polysaccharides were analyzed. Results The polysaccharide from Nostoc commune had certain antioxidant capacity, and its antioxidant capacity showed a good dose-effect relationship within a certain concentration range. The polysaccharide had the highest clearance rate of ·OH, up to 70.53%, followed by DPPH, up to 67.72%, and the lowest removal rate of O_2-, up to 32.31%. The Nostoc communepolysaccharide had a certain inhibitory effect on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Saccharomyces cerevisiae, among which the antibacterial effect on E. coli was the best, the minimum inhibitory mass concentration was 31.25 μg/mL, and it had no inhibitory effect on Salvia miltiorrhiza wilt. ConclusionPolysaccharides from Nostoc Commune has certain anti-oxidation ability and antibacterial ability.
引文
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[19]范羽仪,胡征宇,梅洪.不同念珠藻的提取物对酪氨酸酶活性的抑制作用[J].武汉植物学研究,2008,26(2):179-182.Fan YY,Hu ZY,Mei H.Tyrosinase inhibitory activities of methanolic extracts from different nostoc species[J].J Wuhan Botan Res,2008,26(2):179-182.
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[2]贺宝珍,李义先.地木耳营养价值及利用潜能研究[J].山西大学学报(自然科学版),1991,14(1):93-96.He BZ,Li YX.Study on nutritional value and utilization potential of Nostoc commune[J].J Shanxi Univ(Nat Sci Ed),1991,14(1):93-96.
[3]Yoshimura H,Kotake T,Aohara T,et al.The role of extracellular polysaccharides produced by the terrestrial cyanobacterium Nostoc sp.strain HK-01 in NaCl tolerance[J].J Appl Phycol,2012,24(2):237-243.
[4]Soule T,Shipe D,Lothamer J,et al.Extracellular polysaccharide production in a scytonemin-deficient mutant of Nostoc punctiforme under UVA and oxidative stress[J].Curr Microbiol,2016,73(4):455-456.
[5]张唐伟,柳青海,李天才.地木耳多糖的提取与纯化研究[J].中国野生植物资源,2011,30(3):34-37.Zhang TW,Liu QH,Li TC.Study on isolation and purification of polysaccharides extracted from Nostoc commune Vauch[J].Chin Wild Plant Res,2011,30(3):34-37.
[6]刘继超,刘晓风,张璇.地木耳多糖热水提取工艺优化[J].分子植物育种,2018,16(13):4425-4430.Liu JC,Liu XF,Zhang X.Optimization of the hot water extraction technology of polysaccharide from Nostoc commune[J].Mol Plant Breed,2018,16(13):4425-4430.
[7]范群艳,吴向阳,仰榴青.响应面分析法优化地木耳多糖提取工艺的研究[J].江苏大学学报(医学版),2007,17(3):236-240.Fan QY,Wu XY,Yang LQ.Study on extraction of Nostoc commune Vauch polysaccharides by response surface methodology[J].J Jiangsu Univ(Med Ed),2007,17(3):236-240.
[8]谷绒.超声波和微波法对地木耳多糖提取量的比较[J].食品研究与开发,2010,31(3):23-25.Gu R.compare extraction capacity of auricularia polysaccharides by ultrasonic and microwave[J].Food Res Dev,2010,31(3):23-25.
[9]王迪,王伟,牟洪举,等.响应面法优化松茸菌丝体多糖超声波提取工艺及其抗氧化研究[J].林产化学与工业,2009,29(5):109-114.Wang D,Wang W,Mou HJ,et al.Optimization of ultrasound-assisted extraction of tricholomamatsu take mycelium polysaccharide using response surface methology and its antioxidative effect[J].Chem Ind Forest Prod,2009,29(5):109-144.
[10]Chen YY,Gu XH,Huang SQ,et al.Optimization of ultrasonic/microwave assisted extraction(UMAE)of its anti-tumoractivities[J].Int J Biol Macromol,2010,46(4):429-435.
[11]吴东儒.糖类的生物化学[M].北京:高等教育出版社,1993.Wu DR.Biochemistry of carbohydrates[M].Beijing:Higher Education Press,1993.
[12]刘继攀,李佳欢,张紫华,等.真姬菇水溶性多酚的提取及抗氧化活性研究[J].中国食品添加剂,2018,(1):110-118.Liu JP,Li JH,Zhang ZH,et al.Study on extraction and antioxidant activity of water solvable polyphenols from Hypsizygus marmoreus[J].China Food Add,2018(1):110-118.
[13]刘晓艳,陈艺煊,吴林秀,等.响应面法优化灵芝总三萜酶辅助提取工艺及其抗氧化活性研究[J].食品科技,2017,42(8):225-230.Liu XY,Chen YX,Wu LX,et al.Response surface optimization of enzyme-assisted extraction triterpene from Juncao Ganoderma lucidum and its antioxidant activity[J].Food Sci Technol,2017,42(8):225-230.
[14]Hua SZ,Yina JY,Niea SP,et al.In vitro evaluation of the antioxidant activities of carbohydrates[J].Bioactive Carbohydr Diet Fibre,2016,7(2):19-27.
[15]梁云.几种天然抗氧化剂抗氧化性能比较研究[D].无锡:江南大学,2008.Liang Y.Comparative study on antioxidant properties of several natural antioxidants[D].Wuxi:Jiangnan University,2008.
[16]Huang XQ,Tu ZC,JiangY,et al.Dynamic high pressure microfluidization-assisted extraction and antioxidant activities of lentinan[J].Int J Biol Macromolec,2012,51(5):926-932
[17]王永宁,石玉平,郭珍.沙枣花中黄铜类化合物对羟自由基的清除研究[J].青海医学院学报,2003,24(4):281-283.Wang YN,Shi YP,Guo Z.Scavenging effect of brass compounds on hydroxyl radicals in Elaeagnus angustifolia[J].J Qinghai Med Colleg,2003,24(4):281-283.
[18]吴雪辉,张喜梅,李延群,等.板栗花粗提物的抗氧化活性研究[J].现代食品科技,2008,24(1):14-19.Wu XH,Zhang XM,Li YQ,et al.Study on antioxidant activity of crude extract from chestnut flower[J].Mod Food Sci Technol,2008,24(1):14-19.
[19]范羽仪,胡征宇,梅洪.不同念珠藻的提取物对酪氨酸酶活性的抑制作用[J].武汉植物学研究,2008,26(2):179-182.Fan YY,Hu ZY,Mei H.Tyrosinase inhibitory activities of methanolic extracts from different nostoc species[J].J Wuhan Botan Res,2008,26(2):179-182.
[20]卫生部卫生执法与监督司.消毒技术规范[M].北京:中国人民共和国卫生部,2002.Department of Health Law Enforcement and Supervision of the Ministry of Health.Disinfection specification[M].Beijing:Ministry of Health,People's Republic of China,2002.
[21]Kajiyama SI,Kanzakt H,Kawazu K.Nostofungicidine,an anti-fungal Lipopeptide from the field-grown terrestrial blue-green alga Nostoc commune[J].Tetrahedron Lett,1998,39(22):3737-3740.