采用一测多评法同时测定人参-柴胡药对及其制剂中5种皂苷成分
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摘要
目的采用一测多评法对临床常用药对人参-柴胡及其制剂中的人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、柴胡皂苷a和柴胡皂苷d进行测定。方法以人参皂苷Rg1为参照,建立该成分与其他4种皂苷成分间的相对校正因子,并以此计算其他4种皂苷成分的含量;同时采用外标法实测5种成分的含量进行对比。使用不同色谱柱确定相对保留时间,评价一测多评法在人参-柴胡药对中测定皂苷成分的可行性和准确性。结果建立了5种皂苷成分的定量控制方法,方法学考察结果良好。采用一测多评法及外标法测定人参-柴胡药对、小柴胡汤、开心解郁方中5种皂苷成分,采用t检验分析,一测多评法与外标法结果无显著性差异。结论一测多评法适用于常用药对人参-柴胡中皂苷成分的含量测定,可为复方中人参-柴胡药对有效成分的测定提供借鉴,以及为复方质量控制方法的建立提供参考。
Objective To study the effects of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, saikosaponin a, and saikosaponin d in Ginseng Radix et Rhizoma-Bupleuri Radix(GRRBR) herb pair and its preparations by using quantitative analysis of multi-components by single-marker(QAMS). Methods On the basis of ginsenoside Rg1, the relative correction factors between the ginsenoside Rg1 and the other four saponins were established, and then the contents of the other four saponins were calculated. At the same time, the contents of the five components were determined by external standard method and compared with those evaluated by QAMS. The relative retention time was determined by different chromatographic columns. It could be considered that QAMS was feasible and accurate in the determination of saponins in GRRBR herb pair. Results A quantitative control method of five kinds of saponins was established, and the methodological results were good. The results showed that there was no significant difference in the contents of five kinds of saponins in GRRBR herb pair, Xiaochaihu Decoction, and Kaixin Jieyu Prescription between QAMS group and external standard method group. Conclusion QAMS is suitable for the determination of saponin in GRRBR herb pair, which can be used as a reference for the determination of its effective components and the establishment of compound quality control method.
Objective To study the effects of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, saikosaponin a, and saikosaponin d in Ginseng Radix et Rhizoma-Bupleuri Radix(GRRBR) herb pair and its preparations by using quantitative analysis of multi-components by single-marker(QAMS). Methods On the basis of ginsenoside Rg1, the relative correction factors between the ginsenoside Rg1 and the other four saponins were established, and then the contents of the other four saponins were calculated. At the same time, the contents of the five components were determined by external standard method and compared with those evaluated by QAMS. The relative retention time was determined by different chromatographic columns. It could be considered that QAMS was feasible and accurate in the determination of saponins in GRRBR herb pair. Results A quantitative control method of five kinds of saponins was established, and the methodological results were good. The results showed that there was no significant difference in the contents of five kinds of saponins in GRRBR herb pair, Xiaochaihu Decoction, and Kaixin Jieyu Prescription between QAMS group and external standard method group. Conclusion QAMS is suitable for the determination of saponin in GRRBR herb pair, which can be used as a reference for the determination of its effective components and the establishment of compound quality control method.
引文
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[7]刘小妹,程中琴,施崇精,等.一测多评法同时测定人参中人参皂苷Rb1、Rg1、Re、Rc、Rb2、Rd[J].中草药,2018,49(6):1413-1417.
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[10]蔡皓,庄延双,刘晓,等.正交优选小柴胡汤的提取工艺[J].中药材,2012,35(12):2036-2039.
[11]吕晓霞,陈宗良,陈桂茜,等.一测多评法在仙灵骨葆胶囊中多成分检测的应用研究[J].中草药,2016,47(24):4374-4378.
[12]王嘉林,王斯坦.HPLC-MSMS测定小柴胡颗粒中柴胡皂苷a与柴胡皂苷d[J].安徽医药,2015,19(3):453-456.
[13]陈文霞,武静莲,马东,等.小柴胡颗粒制剂-药材谱峰匹配指纹图谱研究[J].中草药,2013,44(22):3154-3161.
[2]冯琳琳,于敏,李美娟,等.一测多评法测定苦丁茶冬青中6种成分的量[J].中草药,2017,48(14):2955-2959.
[3]王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657-658.
[4]刘潇潇,杨立伟,欧国灯,等.一测多评法在复方丹参片皂苷类成分检测中的应用研究[J].中国新药杂志,2014,23(21):2561-2567.
[5]靳瑞婷,杨素德,付娟,等.一测多评法测定芪白平肺颗粒中8种皂苷类成分[J].中草药,2015,46(24):3682-3686.
[6]张美玲,蔡广知,宋媛,等.基于一测多评法的人参叶药材、提取物及制剂中皂苷测定方法研究[J].药物分析杂志,2015,35(6):997-1001.
[7]刘小妹,程中琴,施崇精,等.一测多评法同时测定人参中人参皂苷Rb1、Rg1、Re、Rc、Rb2、Rd[J].中草药,2018,49(6):1413-1417.
[8]张洪峰,王乐,张凯,等.一测多评法测定冀南产柴胡中柴胡皂苷类成分[J].中国现代中药,2016,18(12):1594-1597.
[9]黄帅,马淼,黄倩倩,等.一测多评法同步测定柴胡药材中3种皂苷的含量[J].时珍国医国药,2010,21(4):838-840.
[10]蔡皓,庄延双,刘晓,等.正交优选小柴胡汤的提取工艺[J].中药材,2012,35(12):2036-2039.
[11]吕晓霞,陈宗良,陈桂茜,等.一测多评法在仙灵骨葆胶囊中多成分检测的应用研究[J].中草药,2016,47(24):4374-4378.
[12]王嘉林,王斯坦.HPLC-MSMS测定小柴胡颗粒中柴胡皂苷a与柴胡皂苷d[J].安徽医药,2015,19(3):453-456.
[13]陈文霞,武静莲,马东,等.小柴胡颗粒制剂-药材谱峰匹配指纹图谱研究[J].中草药,2013,44(22):3154-3161.