植物激素对离体再生的调控及其在南瓜上的作用
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摘要
离体再生涉及植物激素信号应答,细胞分化、脱分化获得组织器官发生以及细胞再分裂形成特定的器官原基和分生组织等过程。激素调控是影响植物离体再生的重要因素。一些关键的启动子元件和转录因子在激素应答信号的检测和转导过程中起着重要作用,激素的控制对象通常是使细胞的形态和代谢发生转变的决定性基因,以转录因子和表观遗传因子为主,它们共同完成对全基因组基因表达的重排,实现细胞命运的转变。该文对植物离体再生中激素的诱导、应答模式和信号转导以及植物器官发生和体细胞胚胎发生过程激素的分子调控进行了综述,并概述了近年来国内外有关南瓜离体再生过程激素作用的研究,旨在为提高南瓜属作物的离体再生率提供新思路。
Regeneration in vitro involves in phytohormone perception, cell differentiation, dedifferentiation to acquire organogenic competence, and organization of cell division to form specific organ primordia and meristems. Hormone regulation is an important factor affecting plant regeneration in vitro. Some key promoter elements and transcription factors play an important role in the process of hormone-response signals detection and transduction. The control object of hormone is usually the crucial genes that change the morphology and metabolism of cells, which are mainly transcription factors and epigenetic factors. They jointly complete the rearrangement of gene expression in genome-wide, and realize cell-fate transition. This review focused on the hormone induction, response patterns, signal transduction of plant regeneration in vitro, hormone molecular regulation in plant organogenesis and somatic embryogenesis, and summarized the role of hormone in pumpkin regeneration in vitro in recent years, aiming to provide a new idea for improving the regeneration rate of Cucurbita crops in vitro.
Regeneration in vitro involves in phytohormone perception, cell differentiation, dedifferentiation to acquire organogenic competence, and organization of cell division to form specific organ primordia and meristems. Hormone regulation is an important factor affecting plant regeneration in vitro. Some key promoter elements and transcription factors play an important role in the process of hormone-response signals detection and transduction. The control object of hormone is usually the crucial genes that change the morphology and metabolism of cells, which are mainly transcription factors and epigenetic factors. They jointly complete the rearrangement of gene expression in genome-wide, and realize cell-fate transition. This review focused on the hormone induction, response patterns, signal transduction of plant regeneration in vitro, hormone molecular regulation in plant organogenesis and somatic embryogenesis, and summarized the role of hormone in pumpkin regeneration in vitro in recent years, aiming to provide a new idea for improving the regeneration rate of Cucurbita crops in vitro.
引文
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3 Su YH,Zhang XS.The hormonal control of regeneration in plants.Curr Top Dev Biol 2014;108:35-69.
4 陶静,詹亚光,由香玲,杨传平,刘玉喜.白桦组培再生系统的研究(Ⅲ)—组培过程中内源激素的变化.东北林业大学学报(Tao Jing,Zhan Yaguang,You Xiangling,Yang Chuanping,Liu Yuxi.Study on tissue culture and regeneration system of Betula platyphylla Suks(Ⅲ):Change of endogenous hormones in tissue culture.Journal of Northeast Forestry University)1998;26(6):6-9.
5 张媛媛,马晖玲,俞玲,赵媛.草地早熟禾愈伤组织诱导和分化中内源激素水平分析.甘肃农业大学学报(Zhang Yuanyuan,Ma Huiling,Yu Ling,Zhao Yuan.Tissue culture and endogenous hormones change of Poa pratensis.Journal of Gansu Agricultural University)2013;48(2):74-9.
6 郭敏敏,王清连,胡根海.利用高效液相色谱法分离和测定棉花组织培养过程中4种内源激素.生物技术通讯(Gou Minmin,Wang Qinglian,Hu Genhai.Separation and determination of four plant hormones during somatic embryogenesis of Gossypium hirsutum L with HPLC.Letters in Biotechnology)2009;20(2):213-6.
7 Nishiwaki M,Fujino K,Koda Y,Masuda K,Kikuta Y.Somatic embryogenesis induced by the simple application of abscisic acid to carrot(Daucus carota L.)seedlings in culture.Planta 2000;211(5):756-9.
8 陈雄,王星,王亚馥.激素对枸杞体细胞胚发生及可溶性蛋白质含量和组分的影响.西北植物学报(Chen Xiong,Wang Xing,Wang Yafu.Effects of hormones on somatic embryogenesis and soluble protein content and components in Lyciumbarbarum L..Acta Botanica Boreali Occidentalia Sinica)1995;15(5):26-30.
9 王秀红,史向远,吴先军,汪旭东,周开达.内源激素对水稻不同外植体培养力的影响.中国农业科学(Wang Xiuhong,Shi Xiangyuan,Wu Xianjun,Wang Xudong,Zhou Kaida.The influence of endogenous hormones on culture capability of different explants in rice.Scientia Agricultura Sinica)2004;37(12):1819-23.
10 李代丽,康向阳.植物愈伤组织培养中内外源激素效应的研究现状与展望.生物技术通讯(Li Dali,Kang Xiangyang.The status and prospect of exodogenous hormone and endogenous hormone impact in plant callus tissue culture.Letters in Biotechnology)2007;18(3):546-8.
11 Feldmann KA,Marks MD.Rapid and efficient regeneration of plants from explants of Arabidopsis thaliana.Plant Sci 1986;47(1):63-9.
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