力学因素对间充质干细胞神经向分化的影响
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摘要
背景:目前,通过对细胞施加机械力学刺激或改变细胞所处的微环境来调控干细胞的分化方向,已经成为了组织工程与再生医学领域的研究热点。目的:综述了近10年来力学因素调控间充质干细胞神经向分化领域的研究进展,为干细胞替代疗法治疗退行性疾病提供相关的理论基础。方法:检索Web of Science和Pub Med数据库中关于力学因素对间充质干细胞神经向分化影响的文献,并进行系统的归纳总结和分析,最终得到104篇相关文献。结果与结论:机械力刺激、细胞外物理环境因素对骨髓间充质干细胞、脂肪间充质干细胞、牙髓干细胞、子宫内膜干细胞等间充质干细胞的神经向分化有重要影响。不同的力学因素可能通过PI3K-AKT-m TOR、Wnt/β-catenin、Rho、MAPK等信号通路调控间充质干细胞的神经向分化,但具体的机制还有待进一步研究。
BACKGROUND: Currently, regulation of stem cell differentiation by mechanical stresses or microenvironment has become a popular issue in the tissue engineering and regenerative medicine. OBJECTIVE: To review the progress in the effects of mechanical stress on neural differentiation of mesenchymal stem cells in the last decade, providing theoretical basis for stem cell replacement therapy in the treatment of degenerative diseases. METHODS: A search of Web of Science and Pub Med database was done to retrieve articles addressing the effects of mechanical stresses on the neural differentiation of mesenchymal stem cells. After systematical analysis and summarization, 104 articles were finally selected and analyzed. RESULTS AND CONCLUSION: Mechanical stimulation and extracellular physical environment factors have important influence on the neural differentiation of mesenchymal stem cells from the bone marrow, adipose tissues, dental pulp and endometrium. PI3 K-AKT-m TOR, Wnt/beta-catenin, Rho, MAPK signaling pathways may be involved in the mechanical stresses regulated neural differentiation. However, the clear molecular mechanism needs further studies.
BACKGROUND: Currently, regulation of stem cell differentiation by mechanical stresses or microenvironment has become a popular issue in the tissue engineering and regenerative medicine. OBJECTIVE: To review the progress in the effects of mechanical stress on neural differentiation of mesenchymal stem cells in the last decade, providing theoretical basis for stem cell replacement therapy in the treatment of degenerative diseases. METHODS: A search of Web of Science and Pub Med database was done to retrieve articles addressing the effects of mechanical stresses on the neural differentiation of mesenchymal stem cells. After systematical analysis and summarization, 104 articles were finally selected and analyzed. RESULTS AND CONCLUSION: Mechanical stimulation and extracellular physical environment factors have important influence on the neural differentiation of mesenchymal stem cells from the bone marrow, adipose tissues, dental pulp and endometrium. PI3 K-AKT-m TOR, Wnt/beta-catenin, Rho, MAPK signaling pathways may be involved in the mechanical stresses regulated neural differentiation. However, the clear molecular mechanism needs further studies.
引文
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[19]Lee HJ,Ewere A,Diaz MF,et al.TAZ responds to fluid shear stress to regulate the cell cycle.Cell Cycle.2017:1-21.
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[23]Jeong SI,Kwon JH,Lim JI,et al.Mechano-active tissue engineering of vascular smooth muscle using pulsatile perfusion bioreactors and elastic PLCL scaffolds.Biomaterials.2005;26(12):1405-1411.
[24]Kim MS,Yeon JH,Park JK.A microfluidic platform for3-dimensional cell culture and cell-based assays.Biomed Microdevices.2007;9(1):25-34.
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