淹水胁迫下褪黑素浸种对水稻幼苗生长的影响
|
摘要
为研究淹水胁迫下褪黑素浸种对长短中胚轴水稻幼苗期生理和生化性状的影响,采用不同浓度梯度的褪黑素(MT)处理,测定中胚轴长、胚芽鞘长、芽长、鲜质量、成活率等相关农艺性状,测定过氧化物歧化酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性以及丙二醛(MDA)含量等生理生化指标。MT浸种对淹水胁迫下水稻生长的影响可分为低浓度缓解胁迫和高浓度加剧毒害;淹水处理下相比短中胚轴品种华航31、DH4040,长中胚轴品种鄂中4号、DH4038具有较高的成活率;在对4个品种添加10,100μmol/L的MT浸种处理时,华航31中MT浸种效果表现均是最好的,其中100μmol/L MT浸种处理在每个品种中均能显著提高幼苗的成活率。随着浓度提高到1 000μmol/L,对所有的品种均表现为抑制成活;MT浸种可降低MDA含量,提高POD、SOD、CAT活性,促进中胚轴长、胚芽鞘长、芽长、鲜质量等农艺性状,100μmol/L MT浸种处理效果最好;1 000μmol/L MT浸种也可以促进相关农艺性状提升并提高酶活性,但成活率显著下降。适当浓度的褪黑素浸种,能提高淹水胁迫下水稻幼苗活力和出苗率,显著降低淹水胁迫对水稻幼苗的毒害作用。
The effects of melatonin-soaking seeds on physiological and biochemical characteristics of rice seedlings under submergence stress. Three concentration treatments of melatonin(10,100,1 000 μmol/L)were arranged. The agronomic traits such as Mesocotyl length,coleoptile length,shoot length,fresh weight and survival rate,and the physiological and biochemical indexes such as enzyme activity of peroxidase(POD),superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)content were measured.The results showed that low concentration MT-soaking could alleviate the stress and high concentration MT-soaking aggravate the toxicity. Compared with short mesocotyl cultivars Huahang 31 and DH4040,long mesocotyl cultivars Ezhong 4 and DH4038 had higher survival rates under submergence stress. Among the four cultivars,Huahang 31 had the best effect of MT-soaking seeds in two treatments,i.e. 10,100 μmol/L MT. The treatment of 100 μmol/L MT-soaking seeds could greatly improve the seedling survival rate of all cultivars. When the MT-soaking concentration increased to 1 000 μmol/L,the survival rate of all cultivars was inhibited. MT-soaking seeds could reduce MDA content,increase POD,SOD and CAT activities,and promote agronomic traits such as mesocotyl length,coleoptile length,bud length and fresh weight,while 100 μmol/L MT-soaking seeds could also promote the related agronomic traits. While 1 000 μmol/L MT-soaking could also promote the related agronomic traits and enzyme activity,but the survival rate decreased significantly.It was concluded that soaking-seeds with appropriate concentration of melatonin could improve the vigor and emergence rate of rice seedlings under submergence stress,and significantly reduce the toxicity of submergence stress on rice seedlings.
The effects of melatonin-soaking seeds on physiological and biochemical characteristics of rice seedlings under submergence stress. Three concentration treatments of melatonin(10,100,1 000 μmol/L)were arranged. The agronomic traits such as Mesocotyl length,coleoptile length,shoot length,fresh weight and survival rate,and the physiological and biochemical indexes such as enzyme activity of peroxidase(POD),superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)content were measured.The results showed that low concentration MT-soaking could alleviate the stress and high concentration MT-soaking aggravate the toxicity. Compared with short mesocotyl cultivars Huahang 31 and DH4040,long mesocotyl cultivars Ezhong 4 and DH4038 had higher survival rates under submergence stress. Among the four cultivars,Huahang 31 had the best effect of MT-soaking seeds in two treatments,i.e. 10,100 μmol/L MT. The treatment of 100 μmol/L MT-soaking seeds could greatly improve the seedling survival rate of all cultivars. When the MT-soaking concentration increased to 1 000 μmol/L,the survival rate of all cultivars was inhibited. MT-soaking seeds could reduce MDA content,increase POD,SOD and CAT activities,and promote agronomic traits such as mesocotyl length,coleoptile length,bud length and fresh weight,while 100 μmol/L MT-soaking seeds could also promote the related agronomic traits. While 1 000 μmol/L MT-soaking could also promote the related agronomic traits and enzyme activity,but the survival rate decreased significantly.It was concluded that soaking-seeds with appropriate concentration of melatonin could improve the vigor and emergence rate of rice seedlings under submergence stress,and significantly reduce the toxicity of submergence stress on rice seedlings.
引文
[1] 凌启鸿.关于水稻轻简栽培问题的探讨[J].中国稻米,1997,3(5):3-9.Ling Q H.Discussion on light and simple cultivation of rice problems[J].China Rice,1997,3(5):3-9.
[2] 李志辉.水稻直播栽培技术要点[J].农村实用科技信息,2012(8):3.doi:10.3969/j.issn.1674-0653.2012.08.002.Li Z H.Key points of direct seeding cultivation techniques in rice[J].Rural Science and Technology Information,2012(8):3.
[3] Angaji S A,Septiningsih E M,Mackill D J.QTLs associated with tolerance of flooding during germination in rice(Oryza sativa L.)[J].Euphytica,2010,172(2):159-168.
[4] Julia B S,Takeshi F,Daniel J Gibbs,Michael J Holdsworth,Seung C L,Francesco L,Pierdomenico P,Laurentius A C J Voesenek,Joost T van D.Making sense of low oxygen sensing[J].Trends in Plant Science,2012,17(3):129-138.doi:10.1016/j.tplants.2011.12.004.
[5] 徐春梅,陈丽萍,王丹英,陈松,章秀福,石庆华.低氧胁迫对水稻幼苗根系功能和氮代谢相关酶活性的影响[J].中国农业科学,2016,49(8):1625-1634.doi:10.3864/j.issn.0578-1752.2016.08.020.Xu C M,Chen L P,Wang D Y,Chen S,Zhang X F,Shi Q H.Effects of low oxygen stress on the root function and enzyme activities related to nitrogen metabolism in roots of rice seedlings [J].Chinese Journal of Agricultural Sciences,2016,49(8):1625-1634.
[6] 李玉昌,李阳生,李绍清.淹涝胁迫对水稻生长发育危害与耐淹性机理研究的进展[J].中国水稻科学,1998(S1):70-76.doi:10.16819/j.1001-7216.1998.s1.013.Li Y C,Li Y S,Li S Q.Progress in research on injury to rice growth and development under submergence stress and mechanism of submergence tolerance [J].Chinese Journal of Rice Science,1998(S1):70-76.
[7] 吴麟,张伟伟,葛晓敏,唐罗忠.植物对淹水胁迫的响应机制研究进展[J].世界林业研究,2012,25(6):27-33.doi:10.13348/j.cnki.sjlyyj.2012.06.009.Wu L,Zhang W W,Ge X M,Tang L Z.A review of the response mechanisms of plants to waterlogging stress [J].World Forestry Research,2012,25(6):27-33.
[8] 马丽峰,李佐同,杨克军,赵长江,徐晶宇,杨荣斌,于高波,赵莹,石新新,许艳梅刘承,闫博巍.没顶淹水对敏感性水稻幼苗生长及抗氧化酶活性的影响[J].植物生理学报,2015(7):1082-1090.doi:10.13592/j.cnki.ppj.2015.0029.Ma L F,Li Z T,Yang K J,Zhao C J,Xu J Y,Yang R B,Yu G B,Zhao Y,Shi X X,Xu Y M,Liu C,Yan B W.Effects of submergence on growth and antioxidant enzymes activities of sensitive rice(Oryza sativa)seedlings [J].Acta Physiologica Sinica,2015(7):1082-1090.
[9] 潘澜,薛立.植物淹水胁迫的生理学机制研究进展[J].生态学杂志,2012,31(10):2662-2672.doi:1000-4890(2012)10-2662-11.Pan W,Xue L.Plant physiological mechanisms in adapting to waterlogging stress:A review[J].Chinese Journal of Ecology,2012,31(10):2662-2672.
[10] 田志环.淹涝胁迫对水稻影响的研究进展[J].安徽农业科学,2008,36(1):143-145.doi:10.3969/j.issn.0517-6611.2008.01.043.Tian Z H.Research advance of the influence of submergence stress on rice [J].Anhui Agricultural Sciences,2008,36(1):143-145.
[11] Fu K T,Bailey S J.Submergence tolerance conferred by sub1a is mediated by slr1 and slrl1 restriction of gibberellin responses in rice[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(43):16814-16819.doi:10.1073/pnas.0807821105.
[12] Fu K T,Xu K,Ronald P C,Bailey S J.A variable cluster of ethylene response factor like genes regulates metabolic and developmental acclimation responses to submergence in rice[J].Plant Cell,2006,18(8):2021-2034.doi:10.1105/tpc.106.043000.
[13] Nandi S,Subudhi P K,Senadhira D,Manigbas N L,Sen-Mandi S,Huang N.Mapping QTLs for submergence tolerance in rice by AFLP analysis and selective genotyping[J].Molecular & General Genetics Mgg,1997,255(1):1.doi:10.1007/s004380050468.
[14] Endang M S,Alvaro M P,Darlene L S,Chirravuri N N,Georgina V V,Sigrid H,Abdelbagi M I,David J M.Development of submergence-tolerant rice cultivars:the Sub1 locus and beyond[J].Annals of Botany,2009,103(2):151-160.doi:10.1093/aob/mcn206.
[15] Toojinda T,Siangliw M,Tragoonrung S,Vanavichit A.Molecular genetics of submergence tolerance in rice:QTL analysis of key traits[J].Annals of Botany,2003,91(2):243-253.doi:org/10.1093/aob/mcf072.
[16] Arnao,Marino B,Hern ndezruiz.Melatonin:plant growth regulator and/or biostimulator during stress?[J].Trends in Plant Science,2014,19(12):789-797.doi:10.1016/j.wasman.2016.06.040.
[17] 赵燕,王东华,赵曦阳.植物中褪黑素的研究进展[J].西北植物学报,2014,34(1):196-205.doi:10.7606/j.issn.1000-4025.2014.01.0196.Zhao Y,Wang D H,Zhao Y Y.Recent advance on the melatonin in plants[J].Northwest Botanical Journal,2014,34(1):196-205.
[18] Hernándezruiz J,Cano A,Arnao M B.Melatonin:a growth-stimulating compound present in lupin tissues.[J].Planta,2004,220(1):140-144.doi:10.1007/s00425-004-1317-3.
[19] Kim M,Seo H,Park C,Park W J.Examination of the auxin hypothesis of phytomelatonin action in classical auxin assay systems in maize[J].Journal of Plant Physiology,2016,190:67-71.doi:10.1016/j.jplph.2015.11.009.
[20] Zhang N,Sun Q Q,Zhang H J,Cao Y Y,Weeda S,Ren S X,Guo Y D.Roles of melatonin in abiotic stress resistance in plants[J].Journal of Experimental Botany,2015,66(3):647.doi:10.1093/jxb/eru336.
[21] 王蕊,杨小龙,须晖,李天来.高等植物褪黑素的合成和代谢研究进展[J].植物生理学报,2016,52(5):615-627.doi:10.13592/j.cnki.ppj.2016.0052.Wang R,Yang X L,Xu H,Li T L.Research progress of melatonin biosynthesis and metabolism in higher plants[J].Acta Physiologica Sinica,2016,52(5):615-627.
[22] 徐芳,周海鹏,郭早霞,于宏安,袁玉川,巩智刚,王玉华.植物褪黑素及其抗逆性研究[J].基因组学与应用生物学,2013,32(2):260-266.doi:10.3969/gab.032.000260.Xu F,Zhou H P,Guo Z X,Yu H A,Yuan Y C,Gong Z G,Wang Y H.The Melatonin and its resistance to stress in plants [J].Genomics and Applied Biology,2013,32(2):260-266.
[23] Tan D X,Hardeland R,Manchester L C,Korkmaz A,Ma S,Rosales-Corral S,Reiter R J.Functional roles of melatonin in plants,and perspectives in nutritional and agricultural science[J].Journal of Experimental Botany,2012,63(2):577.doi:10.1093/jxb/err256.
[24] 叶君,邓西平,王仕稳,殷俐娜,陈道钳,熊炳霖,王鑫月.干旱胁迫下褪黑素对小麦幼苗生长、光合和抗氧化特性的影响[J].麦类作物学报,2015,35(9):1275-1283.doi:10.7606/j.issn.1009-1041.2015.09.15.Ye J,Deng X P,Wang S W,Yin L N,Chen D Q,Xiong B L,Wang X Y.Effects of melatonin on growth,photosynthetic characteristics and antioxidant system in seedling of wheat under drought stress [J].Journal of Triticeae Crops,2015,35(9):1275-1283.
[25] Wang P,Yin L,Liang D,Li C,Ma F,Yue Z.Delayed senescence of apple leaves by exogenous melatonin treatment:toward regulating the ascorbate-glutathione cycle[J].Journal of Pineal Research,2012,53(1):11-20.doi:10.1111/j.1600-079x.2011.00966.x.
[26] Arnao M B,Hern ndez R J.Melatonin promotes adventitious and lateral root regeneration in etiolated hypocotyls of Lupinus albus L.[J].Journal of Pineal Research,2007,42(2):147-152.doi:10.1111/j.1600-079X.2006.00396.x.
[27] Lei X Y,Zhu R Y,Zhang G Y,Dai Y R.Attenuation of cold-induced apoptosis by exogenous melatonin in carrot suspension cells:the possible involvement of polyamines[J].Journal of Pineal Research,2010,36(2):126-131.doi:10.1046/j.1600-079X.2003.00106.x.
[28] Park S,Byeon Y,Back K.Functional analyses of three ASMT gene family members in rice plants[J].Journal of Pineal Research,2013,55(4):409-415.doi:10.1111/jpi.12088.
[29] Reiter R J,Tan D X,Zhou Z,Cruz M H,Fuentes B L,Galano A.Phytomelatonin:assisting plants to survive and thrive[J].Molecules,2015,20(4):7396-7437.doi:10.3390/molecules20047396.
[30] 张娜,蒋庆,李殿波,蔡林桐,张海军,司文君,范希峰,郭仰东.外源施加褪黑素对NaCl胁迫下狼尾草种子萌发及相关生理指标的影响[J].中国农业大学学报,2014,19(3):54-60.doi:10.11841/j.issn.1007-4333.2014.04.08.Zhang N,Jiang Q,Li D B,Cai L T,Zhang H J,Si W J,Fan X F,Guo Y D.Effect of exogenous melatonin on germination of Pennisetum alopecuroides under NaCl stress [J].Journal of China Agricultural University,2014,19(3):54-60.
[31] 高青海,贾双双,苗永美,陆晓民,李慧敏.亚低温条件下外源褪黑素对甜瓜幼苗氮代谢及渗透调节物质的影响[J].应用生态学报,2016,27(2):519-524.doi:10.13287/j.1001-9332.201602.016.Gao Q H,Jia S S,Miao Y M,Lu X M,Li H M.Effects of exogenous melatonin on nitrogen metabolism and osmotic adjustment substances of melon seedlings under sub-low temperature.[J].Chinese Journal of Applied Ecology,2016,27(2):519-524.
[32] 吴丽华,陈燕飞,陈鹏,仪慧兰.亚砷酸钠对酵母细胞抗氧化酶活性和脂质过氧化的影响[J].生态毒理学报,2016,11(3):302-307.doi:10.7524/AJE.1673-5897.20151105001.Wu L H,Chen Y F,Chen P,Yi H L.Sodium arsenite exposure affects the levels of antioxidant enzymes and lipid peroxidation in Saccharomyces cerevisiae [J].Journal of Ecotoxicology,2016,11(3):302-307.
[33] Ren X,Pan L,Wang L.Toxic effects upon exposure to benzo[a]pyrene in juvenile white shrimp Litopenaeus vannamei[J].Environ Toxicol Pharmacol,2015,39(1):194-207.doi:10.1016/j.etap.2014.08.006.
[34] Upadhyay R K.Oxidative injury and its detoxification in rice plants after submergence stress[J].Proceedings of the National Academy of Sciences India,2016,88(5):1-7.doi:10.1007/s40011-016-0724-0.
[35] Zhang H J,Zhang N,Yang R C,Wang L,Sun Q Q,Li D B,Cao Y Y,Weeda S,Zhao B,Ren S,Guo Y D.Melatonin promotes seed germination under high salinity by regulating antioxidant systems,ABA and GA4 interaction in cucumber(Cucumis sativus L.)[J].Journal of Pineal Research,2015,57(3):269-279.doi:10.1111/jpi.12167.
[36] 刘仕翔,黄益宗,罗泽娇,黄永春,保琼莉,王培培,袁彪,李文华.外源褪黑素处理对镉胁迫下水稻种子萌发的影响[J].农业资源与环境学报,2016,35(6):1034-1041.Liu S X,Huang Y Z,Luo Z J,Huang Y C,Bao Q L,Wang P P,Yuan B,Li W H.Effects of exogenous melatonin on germination of rice seeds under Cd stresses [J].Journal of Agricultural Resources and Environment,2016,35(6):1034-1041.doi:10.11654/jaes.2016.06.003.
[37] 熊怀阳,阳菁,安保光.水稻适应淹水胁迫的分子机理及品种改良[J].武汉大学学报:理学版,2013,59(1):17-23.doi:1671-8836(2013)01-0017-07.Xiong H Y,Yang J,An B G.Molecular mechanism of rice adaptation and improvement strategies to submergence stress [J].Journal of Wuhan University,2013,59(1):17-23.
[38] Kanazawa S,Sano S,Koshiba T,Ushimaru T.Changes in antioxidative enzymes in cucumber cotyledons during natural senescence:comparison with those during dark-induced senescence[J].Physiologia Plantarum,2010,109(2):211-216.doi:10.1034/j.1399-3054.2000.100214.x.
[39] 张娜.褪黑素处理对渗透胁迫下黄瓜种子萌发及幼苗生长的影响及其分子机制[D].北京:中国农业大学,2014.Zhang N.Regulation of melatonin on germination and seedling growth under osmotic stress in cucumber [D].Beijing:China Agricultural University,2014.
[2] 李志辉.水稻直播栽培技术要点[J].农村实用科技信息,2012(8):3.doi:10.3969/j.issn.1674-0653.2012.08.002.Li Z H.Key points of direct seeding cultivation techniques in rice[J].Rural Science and Technology Information,2012(8):3.
[3] Angaji S A,Septiningsih E M,Mackill D J.QTLs associated with tolerance of flooding during germination in rice(Oryza sativa L.)[J].Euphytica,2010,172(2):159-168.
[4] Julia B S,Takeshi F,Daniel J Gibbs,Michael J Holdsworth,Seung C L,Francesco L,Pierdomenico P,Laurentius A C J Voesenek,Joost T van D.Making sense of low oxygen sensing[J].Trends in Plant Science,2012,17(3):129-138.doi:10.1016/j.tplants.2011.12.004.
[5] 徐春梅,陈丽萍,王丹英,陈松,章秀福,石庆华.低氧胁迫对水稻幼苗根系功能和氮代谢相关酶活性的影响[J].中国农业科学,2016,49(8):1625-1634.doi:10.3864/j.issn.0578-1752.2016.08.020.Xu C M,Chen L P,Wang D Y,Chen S,Zhang X F,Shi Q H.Effects of low oxygen stress on the root function and enzyme activities related to nitrogen metabolism in roots of rice seedlings [J].Chinese Journal of Agricultural Sciences,2016,49(8):1625-1634.
[6] 李玉昌,李阳生,李绍清.淹涝胁迫对水稻生长发育危害与耐淹性机理研究的进展[J].中国水稻科学,1998(S1):70-76.doi:10.16819/j.1001-7216.1998.s1.013.Li Y C,Li Y S,Li S Q.Progress in research on injury to rice growth and development under submergence stress and mechanism of submergence tolerance [J].Chinese Journal of Rice Science,1998(S1):70-76.
[7] 吴麟,张伟伟,葛晓敏,唐罗忠.植物对淹水胁迫的响应机制研究进展[J].世界林业研究,2012,25(6):27-33.doi:10.13348/j.cnki.sjlyyj.2012.06.009.Wu L,Zhang W W,Ge X M,Tang L Z.A review of the response mechanisms of plants to waterlogging stress [J].World Forestry Research,2012,25(6):27-33.
[8] 马丽峰,李佐同,杨克军,赵长江,徐晶宇,杨荣斌,于高波,赵莹,石新新,许艳梅刘承,闫博巍.没顶淹水对敏感性水稻幼苗生长及抗氧化酶活性的影响[J].植物生理学报,2015(7):1082-1090.doi:10.13592/j.cnki.ppj.2015.0029.Ma L F,Li Z T,Yang K J,Zhao C J,Xu J Y,Yang R B,Yu G B,Zhao Y,Shi X X,Xu Y M,Liu C,Yan B W.Effects of submergence on growth and antioxidant enzymes activities of sensitive rice(Oryza sativa)seedlings [J].Acta Physiologica Sinica,2015(7):1082-1090.
[9] 潘澜,薛立.植物淹水胁迫的生理学机制研究进展[J].生态学杂志,2012,31(10):2662-2672.doi:1000-4890(2012)10-2662-11.Pan W,Xue L.Plant physiological mechanisms in adapting to waterlogging stress:A review[J].Chinese Journal of Ecology,2012,31(10):2662-2672.
[10] 田志环.淹涝胁迫对水稻影响的研究进展[J].安徽农业科学,2008,36(1):143-145.doi:10.3969/j.issn.0517-6611.2008.01.043.Tian Z H.Research advance of the influence of submergence stress on rice [J].Anhui Agricultural Sciences,2008,36(1):143-145.
[11] Fu K T,Bailey S J.Submergence tolerance conferred by sub1a is mediated by slr1 and slrl1 restriction of gibberellin responses in rice[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(43):16814-16819.doi:10.1073/pnas.0807821105.
[12] Fu K T,Xu K,Ronald P C,Bailey S J.A variable cluster of ethylene response factor like genes regulates metabolic and developmental acclimation responses to submergence in rice[J].Plant Cell,2006,18(8):2021-2034.doi:10.1105/tpc.106.043000.
[13] Nandi S,Subudhi P K,Senadhira D,Manigbas N L,Sen-Mandi S,Huang N.Mapping QTLs for submergence tolerance in rice by AFLP analysis and selective genotyping[J].Molecular & General Genetics Mgg,1997,255(1):1.doi:10.1007/s004380050468.
[14] Endang M S,Alvaro M P,Darlene L S,Chirravuri N N,Georgina V V,Sigrid H,Abdelbagi M I,David J M.Development of submergence-tolerant rice cultivars:the Sub1 locus and beyond[J].Annals of Botany,2009,103(2):151-160.doi:10.1093/aob/mcn206.
[15] Toojinda T,Siangliw M,Tragoonrung S,Vanavichit A.Molecular genetics of submergence tolerance in rice:QTL analysis of key traits[J].Annals of Botany,2003,91(2):243-253.doi:org/10.1093/aob/mcf072.
[16] Arnao,Marino B,Hern ndezruiz.Melatonin:plant growth regulator and/or biostimulator during stress?[J].Trends in Plant Science,2014,19(12):789-797.doi:10.1016/j.wasman.2016.06.040.
[17] 赵燕,王东华,赵曦阳.植物中褪黑素的研究进展[J].西北植物学报,2014,34(1):196-205.doi:10.7606/j.issn.1000-4025.2014.01.0196.Zhao Y,Wang D H,Zhao Y Y.Recent advance on the melatonin in plants[J].Northwest Botanical Journal,2014,34(1):196-205.
[18] Hernándezruiz J,Cano A,Arnao M B.Melatonin:a growth-stimulating compound present in lupin tissues.[J].Planta,2004,220(1):140-144.doi:10.1007/s00425-004-1317-3.
[19] Kim M,Seo H,Park C,Park W J.Examination of the auxin hypothesis of phytomelatonin action in classical auxin assay systems in maize[J].Journal of Plant Physiology,2016,190:67-71.doi:10.1016/j.jplph.2015.11.009.
[20] Zhang N,Sun Q Q,Zhang H J,Cao Y Y,Weeda S,Ren S X,Guo Y D.Roles of melatonin in abiotic stress resistance in plants[J].Journal of Experimental Botany,2015,66(3):647.doi:10.1093/jxb/eru336.
[21] 王蕊,杨小龙,须晖,李天来.高等植物褪黑素的合成和代谢研究进展[J].植物生理学报,2016,52(5):615-627.doi:10.13592/j.cnki.ppj.2016.0052.Wang R,Yang X L,Xu H,Li T L.Research progress of melatonin biosynthesis and metabolism in higher plants[J].Acta Physiologica Sinica,2016,52(5):615-627.
[22] 徐芳,周海鹏,郭早霞,于宏安,袁玉川,巩智刚,王玉华.植物褪黑素及其抗逆性研究[J].基因组学与应用生物学,2013,32(2):260-266.doi:10.3969/gab.032.000260.Xu F,Zhou H P,Guo Z X,Yu H A,Yuan Y C,Gong Z G,Wang Y H.The Melatonin and its resistance to stress in plants [J].Genomics and Applied Biology,2013,32(2):260-266.
[23] Tan D X,Hardeland R,Manchester L C,Korkmaz A,Ma S,Rosales-Corral S,Reiter R J.Functional roles of melatonin in plants,and perspectives in nutritional and agricultural science[J].Journal of Experimental Botany,2012,63(2):577.doi:10.1093/jxb/err256.
[24] 叶君,邓西平,王仕稳,殷俐娜,陈道钳,熊炳霖,王鑫月.干旱胁迫下褪黑素对小麦幼苗生长、光合和抗氧化特性的影响[J].麦类作物学报,2015,35(9):1275-1283.doi:10.7606/j.issn.1009-1041.2015.09.15.Ye J,Deng X P,Wang S W,Yin L N,Chen D Q,Xiong B L,Wang X Y.Effects of melatonin on growth,photosynthetic characteristics and antioxidant system in seedling of wheat under drought stress [J].Journal of Triticeae Crops,2015,35(9):1275-1283.
[25] Wang P,Yin L,Liang D,Li C,Ma F,Yue Z.Delayed senescence of apple leaves by exogenous melatonin treatment:toward regulating the ascorbate-glutathione cycle[J].Journal of Pineal Research,2012,53(1):11-20.doi:10.1111/j.1600-079x.2011.00966.x.
[26] Arnao M B,Hern ndez R J.Melatonin promotes adventitious and lateral root regeneration in etiolated hypocotyls of Lupinus albus L.[J].Journal of Pineal Research,2007,42(2):147-152.doi:10.1111/j.1600-079X.2006.00396.x.
[27] Lei X Y,Zhu R Y,Zhang G Y,Dai Y R.Attenuation of cold-induced apoptosis by exogenous melatonin in carrot suspension cells:the possible involvement of polyamines[J].Journal of Pineal Research,2010,36(2):126-131.doi:10.1046/j.1600-079X.2003.00106.x.
[28] Park S,Byeon Y,Back K.Functional analyses of three ASMT gene family members in rice plants[J].Journal of Pineal Research,2013,55(4):409-415.doi:10.1111/jpi.12088.
[29] Reiter R J,Tan D X,Zhou Z,Cruz M H,Fuentes B L,Galano A.Phytomelatonin:assisting plants to survive and thrive[J].Molecules,2015,20(4):7396-7437.doi:10.3390/molecules20047396.
[30] 张娜,蒋庆,李殿波,蔡林桐,张海军,司文君,范希峰,郭仰东.外源施加褪黑素对NaCl胁迫下狼尾草种子萌发及相关生理指标的影响[J].中国农业大学学报,2014,19(3):54-60.doi:10.11841/j.issn.1007-4333.2014.04.08.Zhang N,Jiang Q,Li D B,Cai L T,Zhang H J,Si W J,Fan X F,Guo Y D.Effect of exogenous melatonin on germination of Pennisetum alopecuroides under NaCl stress [J].Journal of China Agricultural University,2014,19(3):54-60.
[31] 高青海,贾双双,苗永美,陆晓民,李慧敏.亚低温条件下外源褪黑素对甜瓜幼苗氮代谢及渗透调节物质的影响[J].应用生态学报,2016,27(2):519-524.doi:10.13287/j.1001-9332.201602.016.Gao Q H,Jia S S,Miao Y M,Lu X M,Li H M.Effects of exogenous melatonin on nitrogen metabolism and osmotic adjustment substances of melon seedlings under sub-low temperature.[J].Chinese Journal of Applied Ecology,2016,27(2):519-524.
[32] 吴丽华,陈燕飞,陈鹏,仪慧兰.亚砷酸钠对酵母细胞抗氧化酶活性和脂质过氧化的影响[J].生态毒理学报,2016,11(3):302-307.doi:10.7524/AJE.1673-5897.20151105001.Wu L H,Chen Y F,Chen P,Yi H L.Sodium arsenite exposure affects the levels of antioxidant enzymes and lipid peroxidation in Saccharomyces cerevisiae [J].Journal of Ecotoxicology,2016,11(3):302-307.
[33] Ren X,Pan L,Wang L.Toxic effects upon exposure to benzo[a]pyrene in juvenile white shrimp Litopenaeus vannamei[J].Environ Toxicol Pharmacol,2015,39(1):194-207.doi:10.1016/j.etap.2014.08.006.
[34] Upadhyay R K.Oxidative injury and its detoxification in rice plants after submergence stress[J].Proceedings of the National Academy of Sciences India,2016,88(5):1-7.doi:10.1007/s40011-016-0724-0.
[35] Zhang H J,Zhang N,Yang R C,Wang L,Sun Q Q,Li D B,Cao Y Y,Weeda S,Zhao B,Ren S,Guo Y D.Melatonin promotes seed germination under high salinity by regulating antioxidant systems,ABA and GA4 interaction in cucumber(Cucumis sativus L.)[J].Journal of Pineal Research,2015,57(3):269-279.doi:10.1111/jpi.12167.
[36] 刘仕翔,黄益宗,罗泽娇,黄永春,保琼莉,王培培,袁彪,李文华.外源褪黑素处理对镉胁迫下水稻种子萌发的影响[J].农业资源与环境学报,2016,35(6):1034-1041.Liu S X,Huang Y Z,Luo Z J,Huang Y C,Bao Q L,Wang P P,Yuan B,Li W H.Effects of exogenous melatonin on germination of rice seeds under Cd stresses [J].Journal of Agricultural Resources and Environment,2016,35(6):1034-1041.doi:10.11654/jaes.2016.06.003.
[37] 熊怀阳,阳菁,安保光.水稻适应淹水胁迫的分子机理及品种改良[J].武汉大学学报:理学版,2013,59(1):17-23.doi:1671-8836(2013)01-0017-07.Xiong H Y,Yang J,An B G.Molecular mechanism of rice adaptation and improvement strategies to submergence stress [J].Journal of Wuhan University,2013,59(1):17-23.
[38] Kanazawa S,Sano S,Koshiba T,Ushimaru T.Changes in antioxidative enzymes in cucumber cotyledons during natural senescence:comparison with those during dark-induced senescence[J].Physiologia Plantarum,2010,109(2):211-216.doi:10.1034/j.1399-3054.2000.100214.x.
[39] 张娜.褪黑素处理对渗透胁迫下黄瓜种子萌发及幼苗生长的影响及其分子机制[D].北京:中国农业大学,2014.Zhang N.Regulation of melatonin on germination and seedling growth under osmotic stress in cucumber [D].Beijing:China Agricultural University,2014.