甘蓝型油菜种子萌发期耐镉性分析及耐镉材料筛选
|
摘要
为研究镉对甘蓝型油菜幼苗的影响,筛选出耐镉品种,以35个不同遗传背景的甘蓝型油菜品系(种)为材料,用不同浓度(1、5、10 mg/L)镉进行胁迫处理。以相对发芽势、相对正常芽苗率、根长抑制率、苗长抑制率、苗鲜重抑制率和苗含水量抑制率六大指标,通过主成分分析和隶属函数分析科学评价不同甘蓝型油菜品系幼苗耐镉性。结果表明:随着镉处理浓度的增大,胁迫对大部分油菜种子的发芽势有促进作用,而成苗率呈下降趋势;幼苗的根长和苗长的抑制均呈上升趋势;苗鲜重和苗含水量呈下降趋势;综合评价筛选出耐镉品系(种)10个,其中品系C13095-6的镉耐性最强。本研究可为甘蓝型油菜品种耐镉性筛选和耐镉育种提供理论参考。
This study aims at the effects of cadmium on seedlings of rapeseed(Brassica napus L.) and the screening of cadmium tolerant lines(varieties). Taking 35 Brassica napus. L lines(varieties) from different genetic backgrounds as the materials, we carried out the stress treatments under different concentrations ofcadmium(1 mg/L, 5 mg/L and 10 mg/L), respectively; and determined 6 indexes including the relativegermination vigor, the seedling rate, the inhibition rate of root length, the inhibition rate of seedling height, theinhibition rate of seedling fresh weight and the inhibition rate of seedling water content, to evaluate thecadmium tolerance in different rapeseed lines(varieties) by using the principal component and subordinatefunction analysis. The results showed that: with the increase of cadmium concentration, cadmium stressenhanced the germination vigor of most rapeseed lines(varieties), but the seedling rates were inhibited; theinhibition rate of root length and seedling height were raised and the seedling fresh weight and seedling watercontent decreased. 10 lines(varieties) were selected by comprehensive assessment, among which the materialC13095-6 showed the strongest cadmium tolerance. This study provides a reference for screening and breeding of cadmium tolerant rapeseed lines(varieties).
This study aims at the effects of cadmium on seedlings of rapeseed(Brassica napus L.) and the screening of cadmium tolerant lines(varieties). Taking 35 Brassica napus. L lines(varieties) from different genetic backgrounds as the materials, we carried out the stress treatments under different concentrations ofcadmium(1 mg/L, 5 mg/L and 10 mg/L), respectively; and determined 6 indexes including the relativegermination vigor, the seedling rate, the inhibition rate of root length, the inhibition rate of seedling height, theinhibition rate of seedling fresh weight and the inhibition rate of seedling water content, to evaluate thecadmium tolerance in different rapeseed lines(varieties) by using the principal component and subordinatefunction analysis. The results showed that: with the increase of cadmium concentration, cadmium stressenhanced the germination vigor of most rapeseed lines(varieties), but the seedling rates were inhibited; theinhibition rate of root length and seedling height were raised and the seedling fresh weight and seedling watercontent decreased. 10 lines(varieties) were selected by comprehensive assessment, among which the materialC13095-6 showed the strongest cadmium tolerance. This study provides a reference for screening and breeding of cadmium tolerant rapeseed lines(varieties).
引文
[1]崔力拓,耿世刚,李志伟,等.我国农田土壤镉污染现状及防治对策[J].现代农业科技,2006(11):184-185.
[2]顾继光,周启星.镉污染土壤的治理及植物修复[J].生态科学,2002,21(4):352-356.
[3]韦朝阳,陈同斌.重金属超富集植物及植物修复技术研究进展[J].生态学报,2001,21(7):1198-1203.
[4]张治安,王振民,徐克章.Cd胁迫对萌发大豆种子中活性氧代谢的影响[J].农业环境科学学报,2005,24(4):670-673.
[5]张琼.镉对几种常见蔬菜种子萌发影响的研究[J].农业环境科学学报,2006,25(增刊):480-486.
[6]四川省土壤污染状况调查公报[EB/OL].中华环保宣传网,2014.11.
[7]苏德纯,黄焕忠.油菜作为超累积植物修复镉污染的潜力[J].中国环境科学,2002,22(1):48-51.
[8]向丹,焦卫平,苏德纯.不同吸镉能力油菜各器官积镉的差异[J].中国油料作物学报,2009,31(1):29-33.
[9]郭媛,邱财生,龙松华,等.镉胁迫对不同地区亚麻主栽品种种子萌发的影响[J].作物杂志,2015(4):146-151.
[10]王宁,南忠仁,王胜利,等.Cd/Pb胁迫下油菜中重金属的分布、富集及迁移特征[J].兰州大学学报.2012,48(3):18-22.
[11]刘春艳.Cu、Cd单一及复合污染对油菜生长发育的影响[D].安徽芜湖:安徽师范大学,2014:4.
[12]刘文英,张东旭,张永芳,等.不同浓度镉胁迫对3种芥菜型幼苗生理特性的影响[J].山西大同大学学报,2014,30(4):45-48.
[13]王一兴.镉对甘蓝型油菜苗期生长的影响[D].南京:南京农业大学,2010:12.
[14] Hui Yan, Fiona Filardo, Xiaotao Hu, et al. Cadmium stress alters the redox reaction and hormone balance in oilseed rape(Brassica napus L.)leaves[J].Environmental Science and Pollution Research,2015:1-12.
[15]明道绪主编.田间试验机统计分析[M].第二版.北京:科学出版社,2008:114-118.
[16]明道绪主编.高级生物统计[M].北京:中国农业出版社,2006:25-74.
[17]沈奇,秦信蓉,杜才富.不同甘蓝型油菜对铬胁迫的耐受性研究[J].贵州农业科学,2010,38(12):47-49.
[18]陶向新.模糊数学在农业科学中的初步应用[J].沈阳农业大学学报,1982(2):96-107.
[19]陈德明,喻仁培,杨劲松.盐渍条件下小麦抗盐性的隶属函数值法评价[J].土壤学报,2002,39(3):369-374.
[20]周广生,梅方竹,周竹青,等.小麦不同品种耐湿性生理指标综合评价及其预测[J].中国农业科学,2003,36(11):1378-1382.
[21]余家林.农业多元试验统计[M].北京:北京农业大学出版社,1993:141-192.
[2]顾继光,周启星.镉污染土壤的治理及植物修复[J].生态科学,2002,21(4):352-356.
[3]韦朝阳,陈同斌.重金属超富集植物及植物修复技术研究进展[J].生态学报,2001,21(7):1198-1203.
[4]张治安,王振民,徐克章.Cd胁迫对萌发大豆种子中活性氧代谢的影响[J].农业环境科学学报,2005,24(4):670-673.
[5]张琼.镉对几种常见蔬菜种子萌发影响的研究[J].农业环境科学学报,2006,25(增刊):480-486.
[6]四川省土壤污染状况调查公报[EB/OL].中华环保宣传网,2014.11.
[7]苏德纯,黄焕忠.油菜作为超累积植物修复镉污染的潜力[J].中国环境科学,2002,22(1):48-51.
[8]向丹,焦卫平,苏德纯.不同吸镉能力油菜各器官积镉的差异[J].中国油料作物学报,2009,31(1):29-33.
[9]郭媛,邱财生,龙松华,等.镉胁迫对不同地区亚麻主栽品种种子萌发的影响[J].作物杂志,2015(4):146-151.
[10]王宁,南忠仁,王胜利,等.Cd/Pb胁迫下油菜中重金属的分布、富集及迁移特征[J].兰州大学学报.2012,48(3):18-22.
[11]刘春艳.Cu、Cd单一及复合污染对油菜生长发育的影响[D].安徽芜湖:安徽师范大学,2014:4.
[12]刘文英,张东旭,张永芳,等.不同浓度镉胁迫对3种芥菜型幼苗生理特性的影响[J].山西大同大学学报,2014,30(4):45-48.
[13]王一兴.镉对甘蓝型油菜苗期生长的影响[D].南京:南京农业大学,2010:12.
[14] Hui Yan, Fiona Filardo, Xiaotao Hu, et al. Cadmium stress alters the redox reaction and hormone balance in oilseed rape(Brassica napus L.)leaves[J].Environmental Science and Pollution Research,2015:1-12.
[15]明道绪主编.田间试验机统计分析[M].第二版.北京:科学出版社,2008:114-118.
[16]明道绪主编.高级生物统计[M].北京:中国农业出版社,2006:25-74.
[17]沈奇,秦信蓉,杜才富.不同甘蓝型油菜对铬胁迫的耐受性研究[J].贵州农业科学,2010,38(12):47-49.
[18]陶向新.模糊数学在农业科学中的初步应用[J].沈阳农业大学学报,1982(2):96-107.
[19]陈德明,喻仁培,杨劲松.盐渍条件下小麦抗盐性的隶属函数值法评价[J].土壤学报,2002,39(3):369-374.
[20]周广生,梅方竹,周竹青,等.小麦不同品种耐湿性生理指标综合评价及其预测[J].中国农业科学,2003,36(11):1378-1382.
[21]余家林.农业多元试验统计[M].北京:北京农业大学出版社,1993:141-192.