7种干果组合对AD模型大鼠行为学及氧化应激水平的影响
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摘要
目的分析7种干果组合对AD大鼠行为学及氧化应激水平的影响。方法 65只SD大鼠随机分5组(13只/组),分别为低(10%)、中(15%)、高(20%)浓度干果饲料组、假手术对照组、模型对照组。假手术对照组海马区注射盐水,其余组注射β淀粉样蛋白25-35(Aβ_(25-35))造模。造模第7天,假手术对照组、模型对照组给予正常饲料,其余组给予含相应浓度干果的饲料,干预40 d。35～40 d进行Morris水迷宫实验。采用酶联免疫吸咐实验(ELISA)试剂盒检测各组大鼠血清、海马匀浆中的丙二醛(MDA)含量以及过氧化氢酶(CAT)和超氧化物歧化酶(SOD)的活性。结果与假手术对照组比较,模型对照组定向航行实验潜伏期、朝向角增加,目标象限进入次数减少,空间探索实验潜伏期时间增加,原平台象限滞留时间百分比、原平台区域穿越次数减少,血清、海马中SOD、CAT含量减少,MDA含量增加,差异有统计学意义(P<0.05)。与模型对照组比较,高浓度组定向航行实验潜伏期、朝向角减少,目标象限进入次数增加,空间探索实验潜伏期时间减少,原平台象限滞留时间百分比、原平台区域穿越次数增加,血清、海马中SOD、CAT含量减少,MDA含量增加,差异有统计学意义(P<0.05)。结论该干果组合可改善AD大鼠的学习、记忆能力,其机制可能与干果组合增强AD大鼠抗氧化能力有关。
Objective To explore the effects of 7 dried fruits′ combinations on Alzheimer′s Disease(AD) rats model. Methods A total of 65 SD rats were randomly divided into 5 groups(with 13 rats in each group) comprised: Sham surgery, model control, low(10%), medium(15%), and high(20%) concentrations. In the sham group, saline was injected into the hippocampus; while in the rest, Aβ_(25-35) was injected for modeling. On the 7 th day of surgery, the sham operation group and the model group were fed with normal diet, and the other groups were fed with corresponding dried fruit diet continuously for 40 days. On the 35 th to 40 th days, start Morris water maze. The contents of SOD, CAT and MDA in serum and hippocampus were determined by ELISA. Results Compared with the sham operation group, the incubation period and orientation Angle of the model group increased, and the number and time of entering the target quadrant decreased. SOD and CAT in serum and hippocampus decreased and MDA increased, the difference was statistically significant(P<0.05). Compared with the model group, the higher the concentration of dried fruit feed was, the incubation period and orientation Angle were generally in a downward trend, while The Times of entering the target quadrant were on the contrary.CAT content in serum and hippocampus were increased with increasing concentration, MDA content was decreased and SOD content was increased. The SOD, CAT and MDA contents in the serum of the medium concentration group and the serum of the high concentration group and the hippocampus were significantly higher than those of the model group(P<0.05). Conclusion The dried fruit combination was exhibited to be capable of improving the learning, memory and antioxidant capacity of AD model rats to a certain extent.
Objective To explore the effects of 7 dried fruits′ combinations on Alzheimer′s Disease(AD) rats model. Methods A total of 65 SD rats were randomly divided into 5 groups(with 13 rats in each group) comprised: Sham surgery, model control, low(10%), medium(15%), and high(20%) concentrations. In the sham group, saline was injected into the hippocampus; while in the rest, Aβ_(25-35) was injected for modeling. On the 7 th day of surgery, the sham operation group and the model group were fed with normal diet, and the other groups were fed with corresponding dried fruit diet continuously for 40 days. On the 35 th to 40 th days, start Morris water maze. The contents of SOD, CAT and MDA in serum and hippocampus were determined by ELISA. Results Compared with the sham operation group, the incubation period and orientation Angle of the model group increased, and the number and time of entering the target quadrant decreased. SOD and CAT in serum and hippocampus decreased and MDA increased, the difference was statistically significant(P<0.05). Compared with the model group, the higher the concentration of dried fruit feed was, the incubation period and orientation Angle were generally in a downward trend, while The Times of entering the target quadrant were on the contrary.CAT content in serum and hippocampus were increased with increasing concentration, MDA content was decreased and SOD content was increased. The SOD, CAT and MDA contents in the serum of the medium concentration group and the serum of the high concentration group and the hippocampus were significantly higher than those of the model group(P<0.05). Conclusion The dried fruit combination was exhibited to be capable of improving the learning, memory and antioxidant capacity of AD model rats to a certain extent.
引文
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[19] 王妍.3种野生浆果花色苷体外抗氧化活性的相关性研究[J].防护林科技,2013(2):23-26.
[20] 宋根伟,黄博,何敬胜,等.巴旦杏挥发油化学成分和提取物抗氧化作用研究[J].塔里木大学学报,2009,21(3):10-14.
[2] LEONI V.The effect of apolipoprotein E (ApoE) genotype on biomarkers of amyloidogenesis,tau pathology and neurodegeneration in Alzheimer′s disease[J] Clin Chem Lab Med,2011,49(3):375-383.
[3] 艾晓娟,邓治文,王洲,等.脂肪肝泰胶囊对氧四环素加高脂饲料致大鼠非酒精性脂肪肝的影响[J].中药药理与临床,2015(1):187-190.
[4] 史信.《中国居民膳食指南(2016)》发布[J].中国妇幼健康研究,2016,27(5):30-30.
[5] 袁芳.天然药物对阿尔茨海默病干预的研究进展[J].新疆医科大学学报,2014(8):1092-1095.
[6] 刘卓,金英,刘婉珠,等.知母皂苷对脂多糖引起的大鼠学习记忆障碍和炎症反应的影响[J].中国药理学通报,2010,26(10):1362-1366.
[7] 方松.Morris水迷宫实验中海马相关空间学习记忆的研究进展[J].临床与病理杂志,2010,30(4):321-326.
[8] 王爱梅,未小明,李弋,等.旱莲草对老年痴呆模型大鼠空间学习记忆及海马神经元突触可塑性的影响[J].神经解剖学杂志,2015,31(1):73-78.
[9] PADURARIU M,CIOBICA A,HRITCU L,et al.Changes of some oxidative stress markers in the serum of patients with mild cognitive impairment and Alzheimer′s disease[J].Neuroscience Letters,2010,469(1):6-10.
[10] 岳强,游咏.阿尔茨海默病神经炎症机制研究进展[J].医药前沿,2017,7(2):32-34.
[11] IZCO M,MARTíNEZ P,CORRALES A,et al.Changes in the brain and plasma Aβ peptide levels with age and its relationship with cognitive impairment in the APPswe/PS1dE9 mouse model of Alzheimer's disease[J].Neuroscience,2014,263:269-279.
[12] LI H,SONG Y,HE Z,et al.Meclofenamic acid reduces reactive oxygen species accumulation and apoptosis,inhibits excessive autophagy,and protects hair cell-like HEI-OC1 cells from cisplatin-induced damage[J].Front Cell Neurosci,2018,12:139-141.
[13] 王切,杜建文,刘健,等.大鼠肝缺血再灌注损伤肾内过氧化物酶V、过氧化氢酶、超氧化物歧化酶的表达变化[J].解剖学杂志,2015,38(6):660-663.
[14] YANG J,RUI H L,HALIM L.Antioxidant and antiproliferative activities of common edible nut seeds[J].Food Sci Technol,2009,42(1):1-8.
[15] SHEKAARI M,KARIMI A,SHABANI M,et al.Maternal feeding with walnuts (Juglans regia) improves learning and memory in their adult pups[J].AJP,2013,3(4):341.
[16] NATOLI S,MCCOY P.A review of the evidence:nuts and body weight[J].Asia Pac J Clin Nutr,2007,16(4):588-597.
[17] 孙月娥,王卫东,郑义.巴旦木蛋白水解物的制备及其抗氧化活性[J].食品与发酵工业,2011,37(10):105-109.
[18] 贾娜,孔保华,张洪涛.黑加仑花色苷的提取及抗氧化活性研究[J].食品科学,2011(16):162-166.
[19] 王妍.3种野生浆果花色苷体外抗氧化活性的相关性研究[J].防护林科技,2013(2):23-26.
[20] 宋根伟,黄博,何敬胜,等.巴旦杏挥发油化学成分和提取物抗氧化作用研究[J].塔里木大学学报,2009,21(3):10-14.