黄精多糖对大强度运动大鼠外周血肌酸激酶、尿素氮及部分免疫指标的影响
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摘要
目的通过建立大强度运动模型,探讨黄精多糖对大鼠外周血CK、BUN、免疫球蛋白、T淋巴细胞亚群的影响。方法实验大鼠30只,按体重随机分为安静组、运动组、运动给药组,每组10只。运动给药组小鼠灌服剂量为100g/Kg/d黄精多糖溶液,其他两小组按质量灌服相应体积的生理盐水。运动训练组、运动给药组进行为期9周的大强度跑台训练。测定各组大鼠外周血CK、BUN、 IgG、IgA、IgM、 CD~+_3、CD~+_4、CD~+_8、CD~+_4/CD~+_8。结果运动组与安静组相比,CK、BUN升高(P<0.01),CD~+_3、CD~+_4、CD~+_4/CD~+_8、 IgG、IgA、IgM降低(P<0.01)。运动给药组与运动组相比,CK、BUN降低(P<0.01),CD~+_3、CD~+_4、CD~+_8、CD~+_4/CD~+_8、 IgG、IgA、IgM升高(P<0.01)。结论黄精多糖能抑制大强度运动导致的血中CK含量的升高,延缓运动疲劳的发生,提升机体体液免疫和细胞免疫的功能。
Objective To discuss the effects of polygonatum polysaccharide on peripheral blood creatine kinase,urea nitrogen,T lymphocyte subsets and immunoglobulin on the basis of building up a high-intensity exercise model.Methods 30 experimental rats were assigned by weights and randomized into sedentary control group,trained group,dosage plus trained group,which contain 10 rats respectively.Then dosage-given exercise rats were fed with polygonatum polysaccharide solution with the dosages of 100 g/Kg/d,meanwhile,the other two groups were given the same amount physiological saline solution.Except for the sedentary control group,rats from trained group and dosage plus trained group were carried out the high intensity running within the period of 9 weeks to detect the index of peripheral CK,BUN,IgG,IgA,IgM,CD~+_3,CD~+_4,CD~+_8,CD~+_4/CD~+_8.Results compared trained group with sedentary control group,CK and BUN were significantly increased(P<0.01),CD~+_3,CD~+_4,CD~+_4/CD~+_8,IgG,IgA and IgM were significantly decreased(P<0.01),and there is no significant difference in the aspect of CD~+_8;compared dosage plus trained group with trained group,CD~+_3,CD~+_4,CD~+_8,CD~+_4/CD~+_8,IgG,IgA and IgM were significantly increased(P<0.01),and there is no obvious change in CD~+_8.Conclusion Polygonatum polysaccharide can maintain the normal physiological functioning of cells,delay the occurrence of exercise fatigue and enhance the function of humoral immunity and cellular immunity.
Objective To discuss the effects of polygonatum polysaccharide on peripheral blood creatine kinase,urea nitrogen,T lymphocyte subsets and immunoglobulin on the basis of building up a high-intensity exercise model.Methods 30 experimental rats were assigned by weights and randomized into sedentary control group,trained group,dosage plus trained group,which contain 10 rats respectively.Then dosage-given exercise rats were fed with polygonatum polysaccharide solution with the dosages of 100 g/Kg/d,meanwhile,the other two groups were given the same amount physiological saline solution.Except for the sedentary control group,rats from trained group and dosage plus trained group were carried out the high intensity running within the period of 9 weeks to detect the index of peripheral CK,BUN,IgG,IgA,IgM,CD~+_3,CD~+_4,CD~+_8,CD~+_4/CD~+_8.Results compared trained group with sedentary control group,CK and BUN were significantly increased(P<0.01),CD~+_3,CD~+_4,CD~+_4/CD~+_8,IgG,IgA and IgM were significantly decreased(P<0.01),and there is no significant difference in the aspect of CD~+_8;compared dosage plus trained group with trained group,CD~+_3,CD~+_4,CD~+_8,CD~+_4/CD~+_8,IgG,IgA and IgM were significantly increased(P<0.01),and there is no obvious change in CD~+_8.Conclusion Polygonatum polysaccharide can maintain the normal physiological functioning of cells,delay the occurrence of exercise fatigue and enhance the function of humoral immunity and cellular immunity.
引文
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[13] 李旭武.体育舞蹈锻炼对大学生血清免疫球蛋白及T淋巴细胞亚群的影响[J].体育学刊,2014,21(4):135-139.
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[18] 叶绍凡.黄精多糖对力竭运动小鼠胸腺胸腺指数、脾脏指数、T淋巴细胞亚群、巨噬细胞吞噬功能的影响[J].基因组学与应用生物学,2015,34(1):60-65.
[19] 王晓琴,刘坚,袁际学,等.烟盒舞运动对中年女性血清免疫球蛋白和血脂的影响[J].运动,2017(18):155-156.
[20] 周彩华,王献升,莫伟彬,等.益生菌对运动大鼠免疫球蛋白、IL-6、TNF-α含量及HSP70表达的影响[J].现代预防医学,2018,45(10):1835-1838.
[2] 蔡国梁,郭霞,汪宇峰,等.不同负荷游泳运动对大鼠部分免疫指标的影响[J].哈尔滨体育学院学报,2017,35(2):1-5,11.
[3] 李超彦,周媛媛,王福青.黄精多糖联合低剂量顺铂对小鼠H22肝癌移植瘤生长的抑制及其抗氧化损伤作用[J].中国老年学杂志,2016,36(5):1038-1040.
[4] 赵宏丽,许燕,赵红岩,等.黄精多糖对2型糖尿病大鼠SREBP-1c和SCD-1蛋白表达的影响[J].中药药理与临床,2015,31(1):106-109.
[5] Bedford TG,Tipton CM,Wilson NC,et al.Maximum oxygen consumption of rats and its changes with various experimental procedures[J].Journal of Applied Physiology,1979,47(6):1278-1283.
[6] 王茹,陈佩杰.运动诱导的T_H1/T_H2淋巴细胞亚群失衡机制研究进展[J].中国运动医学杂志,2009(1):112-117.
[7] 叶素英,周艳阳,叶绍凡.黄精多糖对大强度训练大鼠血红蛋白、乳酸及脑组织抗氧化能力、一氧化氮体系的影响[J].中国老年学杂志,2014,34(23):6706-6708.
[8] 方欢乐,李晓明,李秋全,等.黄精多糖的提取及抗氧化作用的研究[J].生物化工,2018,4(3):11-12,15.
[9] 刘江,李毅钧.排球多元组合训练的生理生化监控及实施研究——以四川省男子排球队为例[J].广州体育学院学报,2016,36(1):102-105.
[10] 王志锋,高可丽,王东旭.运动应激对新疆少数民族大学生部分生理生化指标的影响[J].中国学校卫生,2016,37(1):11-15.
[11] 李国安,刘毅伟.古典式摔跤运动员夏训训期监测研究[J].武汉体育学院学报,2015,49(4):87-90.
[12] 李明.黄精多糖对运动疲劳大鼠抗氧化及神经递质的影响[J].食品科技,2014,39(9):227-230.
[13] 李旭武.体育舞蹈锻炼对大学生血清免疫球蛋白及T淋巴细胞亚群的影响[J].体育学刊,2014,21(4):135-139.
[14] 聂健,王冀邯.运动对大鼠脾脏T淋巴细胞增殖及T细胞亚群的影响研究[J].中国免疫学杂志,2015,31(4):477-479.
[15] 高明,吴瑛,盛泽田,等.针灸预处理对摔跤运动员大负荷训练阶段NK细胞和T淋巴细胞亚群的影响[J].上海体育学院学报,2014,38(3):67-72.
[16] 卢咏梅,张宏.电针对大强度运动应激大鼠细胞免疫的影响[J].针刺研究,2012,37(2):136-139.
[17] 王文迪.黄精多糖对一次大强度运动后人体外周血T淋巴细胞免疫功能的影响[D].成都:成都体育学院,2012.
[18] 叶绍凡.黄精多糖对力竭运动小鼠胸腺胸腺指数、脾脏指数、T淋巴细胞亚群、巨噬细胞吞噬功能的影响[J].基因组学与应用生物学,2015,34(1):60-65.
[19] 王晓琴,刘坚,袁际学,等.烟盒舞运动对中年女性血清免疫球蛋白和血脂的影响[J].运动,2017(18):155-156.
[20] 周彩华,王献升,莫伟彬,等.益生菌对运动大鼠免疫球蛋白、IL-6、TNF-α含量及HSP70表达的影响[J].现代预防医学,2018,45(10):1835-1838.