昼夜温差处理下铁皮石斛原球茎松柏苷和紫丁香苷含量的测定
|
摘要
研究不同昼夜温差对铁皮石斛原球茎松柏苷、紫丁香苷含量的影响,为开发铁皮石斛原球茎作为药材提供研究基础。采用光照培养箱设置不同温差处理,采用超高效液相色谱法测定松柏苷、紫丁香苷含量变化。建立同时测定松柏苷和紫丁香苷的超高效液相色谱法,以ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)为分析柱;流动相:1%乙酸水溶液(A)和甲醇(C)。正交试验研究提取方法,结果表明用纯水提取,料液比1∶40,提取1 h(每0.5 h震荡一次),2种物质提取率最高。采用此方法测定4个昼夜温差处理的铁皮石斛原球茎样品,结果表明,对照0℃温差松柏苷、紫丁香苷整体变化趋于平缓,8℃温差处理下,松柏苷和紫丁香苷在10 d的处理周期下都表现出含量增加,且明显优于其他处理。4℃温差处理两物质含量在第15天达到最高,12℃温差处理两物质含量前期高于对照处理,处理后期低于对照处理,且低于4℃、8℃温差处理。昼夜温差影响铁皮石斛原球茎松柏苷、紫丁香苷含量,一定范围内的温差呈现促进作用,温差过大则出现抑制作用。
To understand the effects of temperature difference between day and night on the contents of coniferin and syringin in the protocorm of Dendrobium officinale, which would provide a the research basis for the development of protocorm of D. officinale as a medicinal material in large scale production. The fast method based on ultra-high performance liquid chromatography(UPLC) for the analysis of coniferin and syringin, the chromatographic separation was carried on an ACQUITY UPLC BEH C18(2.1 mm × 100 mm, 1.7 μm) in gradient elution with a mobile phase of acetic acid aqueous solution(1%, w/w)(A) and methanol(C). The extraction method of an orthogonal test was designed, and the results showed that the sample extracted with pure water, extraction ratio 1:40, and the extraction time 1 h(vibrate every half an hour) had the highest extraction rate of the two substances. The proposed method was applied to the determination of four samples of D. officinale protocorm in temperature difference treatment. The results showed that the whole change of syringin and coniferin tended to be gental at 0 ℃. The contents of coniferin and syringin increased in the 10 d treatment with temperature difference 8 ℃ between day and night, and they were obviously superior to those of other treatments. The content of the two substances reached the highest on the 15 d treatment with temperature difference 4 ℃ between day and night. The contents of the two substances in the temperature difference 12 ℃ treatment were higher in the early stage than that in the control treatment, and were lower in the latter stage than that in the control treatment, and were also lower than that in the temperature difference 8 ℃ and 4 ℃ treatment. Moderate temperature difference appears to promote the effect, while big temperature difference lowers the effect.
To understand the effects of temperature difference between day and night on the contents of coniferin and syringin in the protocorm of Dendrobium officinale, which would provide a the research basis for the development of protocorm of D. officinale as a medicinal material in large scale production. The fast method based on ultra-high performance liquid chromatography(UPLC) for the analysis of coniferin and syringin, the chromatographic separation was carried on an ACQUITY UPLC BEH C18(2.1 mm × 100 mm, 1.7 μm) in gradient elution with a mobile phase of acetic acid aqueous solution(1%, w/w)(A) and methanol(C). The extraction method of an orthogonal test was designed, and the results showed that the sample extracted with pure water, extraction ratio 1:40, and the extraction time 1 h(vibrate every half an hour) had the highest extraction rate of the two substances. The proposed method was applied to the determination of four samples of D. officinale protocorm in temperature difference treatment. The results showed that the whole change of syringin and coniferin tended to be gental at 0 ℃. The contents of coniferin and syringin increased in the 10 d treatment with temperature difference 8 ℃ between day and night, and they were obviously superior to those of other treatments. The content of the two substances reached the highest on the 15 d treatment with temperature difference 4 ℃ between day and night. The contents of the two substances in the temperature difference 12 ℃ treatment were higher in the early stage than that in the control treatment, and were lower in the latter stage than that in the control treatment, and were also lower than that in the temperature difference 8 ℃ and 4 ℃ treatment. Moderate temperature difference appears to promote the effect, while big temperature difference lowers the effect.
引文
[1]沈洁琼,徐建.铁皮石斛、杯鞘石斛、齿瓣石斛的指纹图谱研究[J].浙江中医杂志,2015,50(7):534-536.
[2]Meng Y,Yu D,Xue J,et al.Atranscriptome-wide,organ-specific regulatory map of Dendrobium officinale,an important traditional Chinese orchid herb[J].Scientific Reports,2016,1(6):1-13.
[3]Zhang J,He C,Wu K,et al.Transcriptome analysis of Dendrobium officinale and its application to the identification of genes associated with polysaccharide synthesis[J].Frontiers in Plant Science,2016,7(5):1-14.
[4]Li C,Qiu J,Ding L,et al.Anthocyanin biosynthesis regulation of DhMYB2 and DhbHLH1 in Dendrobium hybrids petals[J].Plant Physiology&Biochemistry,2017(112):335-345.
[5]刘洁,李创军,杨敬芝.黄皮茎枝化学成分研究[J].中草药,2016(1):32-37.
[6]王岩,张海宁,王文婧.椿皮化学成分的研究[J].中草药,2012(4):649-652.
[7]文成哲,文关心.朝鲜丁香皮的苯丙素酚类成分研究及定量测定[J].中草药,2015(12):1749-1752.
[8]王慧,张红歧,邬昊洋.筒鞘蛇菰提取物及松柏苷的抗氧化作用研究[J].三峡大学学报(自然科学版),2009(3):99-101.
[9]陈日道.天山雪莲培养物的化学成分及紫丁香苷含量研究[D].沈阳:沈阳药科大学,2009.
[10]Niu H S,Liu I M,Cheng J T,et al.Hypoglycemic effect of syringin from Eleutherococcus senticosus in streptozotocin-induced diabetic rats[J].Planta Medica,2008,74(2):109-113.
[11]Choi J,Shin K M,Park H J,et al.Anti-inflammatory and antinociceptive effects of sinapyl alcohol and its glucoside syringin[J].Planta Medica,2004,70(11):1027-1032.
[12]刘圆,李厚聪,孟庆艳.HPLC法测定川产红毛五加不同药用部位和不同产地茎皮中紫丁香苷[J].中草药,2008,(4):612-613.
[13]Chuan L H,Huang H Q,Liu X X,et al.Experimental research on the protective effect of Si Ni decoction on acute ischemic myocardium[J].Chinese Journal of Pathophysiology,1999(11):994-996.
[14]李岩,陈德泉,叶泽波.HPLC法测定铁皮石斛中酚酸类物质组成及含量[J].食品研究与开发,2018,39(7):174-179.
[15]周桂芬,吕圭源.铁皮石斛叶中黄酮碳苷类成分的高效液相指纹图谱及指标成分的含量测定[J].中国药学杂志,2012,47(11):889-893.
[16]周桂芬,吕圭源.基于高效液相色谱-二极管阵列光谱检测-电喷雾离子化质谱联用鉴定铁皮石斛叶中8种黄酮碳苷化合物及裂解规律研究[J].中国药学杂志,2012,47(1):13-19.
[17]周桂芬.高效液相色谱法测定铁皮石斛中柚皮素的含量[J].中国中药杂志,2013,38(4):520-523.
[18]张淋洁,王如伟,何厚洪.高效液相色谱-蒸发光散射法测定铁皮石斛中单糖和双糖[J].医药导报,2013,32(4):517-520.
[19]周桂芬,庞敏霞,陈素红.铁皮石斛茎、叶多糖含量及多糖部位柱前衍生化-高效液相色谱指纹图谱比较研究[J].中国中药杂志,2014(5):795-802.
[20]黄月纯,杨丽娥,刘宏源.鲜铁皮石斛茎、叶高效液相色谱特征图谱比较[J].广州中医药大学学报,2012(5):561-565.
[21]Solich P.Analysis of phenolic compounds by high performance liquid chromatography and ultra performance liquid chromatography[J].Talanta,2008,76(1):189-199.
[22]毛丽萍,李亚灵,温祥珍.苗期昼夜温差对番茄产量形成因子的影响分析[J].农业工程学报,2012(16):172-177.
[23]杨再强,朱凯,彭晓丹.昼夜温差对设施番茄叶片光合特性和叶绿素荧光参数的影响[J].生态学杂志,2013(12):3190-3196.
[24]Bachman G R,Mcmahon M J.Day and night temperature differential(DIF)or the absence of far-red light alters cell elongation in‘Celebrity White’petunia[J].Journal of the American Society for Horticultural Science,2006,131(3):309-312.
[25]Wang Y T.Effects of reversed day/night temperatures on a Doritaenopsis hybrid orchid[J].Hortscience,2004,39(4):834-834.
[26]杨再强,王学林,彭晓丹.人工环境昼夜温差对番茄营养物质和干物质分配的影响[J].农业工程学报,2014(5):138-147.
[27]Papadopoulos A P,Hao X M.Effects of day and night air temperature in early season on growth,productivity and energy use of spring tomato[J].Canadian Journal of Plant Science,2001,81(2):303-311.
[28]邱文伟,张光伦,张嵩.柑橘等果实糖代谢及其生态调控研究进展[J].四川农业大学学报,2005(1):114-119.
[29]陈艳秋,赵翔,刘昭霞.不同昼夜温差对设施番茄果实发育及产量的影响[J].北方园艺,2014(24):38-42.
[30]郑卫杰,郭子霞,王政.昼夜温差对文心兰试管苗生长的影响[J].西北林学院学报,2011,26(4):137-141.
[31]杨博,曹秀婷,王政.昼夜温差对非洲菊试管苗生长的影响[J].西北林学院学报,2012,27(2):88-92.
[32]艾娟,严宁,胡虹.温度对铁皮石斛生长及生理特性的影响[J].云南植物研究,2010,32(5):420-426.
[33]何铁光,苏江,王灿琴.铁皮石斛不同来源材料多糖和氨基酸含量的比较[J].广西农业科学,2007,38(1):32-34.
[34]Chen Z H,Chen Y L,Tao W U,et al.Study on structural characteristics and immunomodulatory activity of polysaccharide DMP4a-1 from Dendrobium moniliforme[J].Chinese Pharmaceutical Journal,2005,40(23):1781-1784.
[35]董思艳.不同叶面肥对铁皮石斛药效成分含量的影响[D].昆明:云南中医学院,2014.
[36]何铁光,杨丽涛,李杨瑞.铁皮石斛原球茎多糖DCPP3c-1的分离纯化及结构初步分析[J].分析测试学报,2008,27(2):143-147.
[37]诸燕,张爱莲,何伯伟.铁皮石斛总生物碱含量变异规律[J].中国中药杂志,2010,35(18):2388-2391.
[38]李燕,王春兰,王芳菲.铁皮石斛中的酚酸类及二氢黄酮类成分[J].中国药学杂志,2010,45(13):975-979.
[39]李燕,王春兰,王芳菲.铁皮石斛化学成分的研究[J].中国中药杂志,2010,35(13):1715-1719.
[40]王芳菲,李燕,董海玲,等.铁皮石斛中一个新化合物[J].中国药学杂志,2010,45(12):898-902.
[41]黄相中.一种提高铁皮石斛中多酚成分浸出率的方法:105560888 A[P].2016-05-11.
[42]郑洁,赵其阳,张耀海.超高效液相色谱法同时测定柑橘中主要酚酸和类黄酮物质[J].中国农业科学,2014,47(23):4706-4717.
[43]冉玥,焦必宁,赵其阳.超高效液相色谱法同时测定柑橘中11种类黄酮物质[J].食品科学,2013,34(4):168-172.
[44]周桂芬,陈素红,吕圭源.浙江省铁皮石斛黄酮类成分高效液相色谱指纹图谱的建立[J].中国药学杂志,2013,48(15):1261-1265.
[45]冯淑艳,董睿,彭黔荣,等.超高效液相色谱法测定贵州烟草中的游离氨基酸[J].化学研究与应用,2018,30(1):54-61.
[46]雷蓉,赵振霞,刘永利,等.超高效液相色谱法同时测定三七的叶和花中3个皂苷类成分含量[J].药物分析杂志,2018,38(7):1146-1151.
[47]白云,朱瑞超,李晋,等.超高效液相色谱法同时测定金花葵花中5个黄酮类成分含量[J].天津中医药,2018,35(2):147-150.
[48]孙存华,毛健民,白宝璋.昼夜变温促进小麦幼苗生长的酶学研究[J].吉林农业大学学报,2000,22(1):30-33.
[49]李莉,李佳,高青,等.昼夜温差对番茄生长发育、产量及果实品质的影响[J].应用生态学报,2015,26(9):2700-2706.
[50]郝敬虹,李天来,孟思达.夜间低温对薄皮甜瓜果实糖积累及代谢相关酶活性的影响[J].中国农业科学,2009,42(10):3592-3599.
[51]张明,夏鸿西,朱利泉.石斛组织培养研究进展[J].中国中药杂志,2000,25(6):3-6.
[52]潘宏兵,代沙,陈云清,等.铁皮石斛大棚种植环境调控技术[J].四川农业科技,2017(9):19-21.
[2]Meng Y,Yu D,Xue J,et al.Atranscriptome-wide,organ-specific regulatory map of Dendrobium officinale,an important traditional Chinese orchid herb[J].Scientific Reports,2016,1(6):1-13.
[3]Zhang J,He C,Wu K,et al.Transcriptome analysis of Dendrobium officinale and its application to the identification of genes associated with polysaccharide synthesis[J].Frontiers in Plant Science,2016,7(5):1-14.
[4]Li C,Qiu J,Ding L,et al.Anthocyanin biosynthesis regulation of DhMYB2 and DhbHLH1 in Dendrobium hybrids petals[J].Plant Physiology&Biochemistry,2017(112):335-345.
[5]刘洁,李创军,杨敬芝.黄皮茎枝化学成分研究[J].中草药,2016(1):32-37.
[6]王岩,张海宁,王文婧.椿皮化学成分的研究[J].中草药,2012(4):649-652.
[7]文成哲,文关心.朝鲜丁香皮的苯丙素酚类成分研究及定量测定[J].中草药,2015(12):1749-1752.
[8]王慧,张红歧,邬昊洋.筒鞘蛇菰提取物及松柏苷的抗氧化作用研究[J].三峡大学学报(自然科学版),2009(3):99-101.
[9]陈日道.天山雪莲培养物的化学成分及紫丁香苷含量研究[D].沈阳:沈阳药科大学,2009.
[10]Niu H S,Liu I M,Cheng J T,et al.Hypoglycemic effect of syringin from Eleutherococcus senticosus in streptozotocin-induced diabetic rats[J].Planta Medica,2008,74(2):109-113.
[11]Choi J,Shin K M,Park H J,et al.Anti-inflammatory and antinociceptive effects of sinapyl alcohol and its glucoside syringin[J].Planta Medica,2004,70(11):1027-1032.
[12]刘圆,李厚聪,孟庆艳.HPLC法测定川产红毛五加不同药用部位和不同产地茎皮中紫丁香苷[J].中草药,2008,(4):612-613.
[13]Chuan L H,Huang H Q,Liu X X,et al.Experimental research on the protective effect of Si Ni decoction on acute ischemic myocardium[J].Chinese Journal of Pathophysiology,1999(11):994-996.
[14]李岩,陈德泉,叶泽波.HPLC法测定铁皮石斛中酚酸类物质组成及含量[J].食品研究与开发,2018,39(7):174-179.
[15]周桂芬,吕圭源.铁皮石斛叶中黄酮碳苷类成分的高效液相指纹图谱及指标成分的含量测定[J].中国药学杂志,2012,47(11):889-893.
[16]周桂芬,吕圭源.基于高效液相色谱-二极管阵列光谱检测-电喷雾离子化质谱联用鉴定铁皮石斛叶中8种黄酮碳苷化合物及裂解规律研究[J].中国药学杂志,2012,47(1):13-19.
[17]周桂芬.高效液相色谱法测定铁皮石斛中柚皮素的含量[J].中国中药杂志,2013,38(4):520-523.
[18]张淋洁,王如伟,何厚洪.高效液相色谱-蒸发光散射法测定铁皮石斛中单糖和双糖[J].医药导报,2013,32(4):517-520.
[19]周桂芬,庞敏霞,陈素红.铁皮石斛茎、叶多糖含量及多糖部位柱前衍生化-高效液相色谱指纹图谱比较研究[J].中国中药杂志,2014(5):795-802.
[20]黄月纯,杨丽娥,刘宏源.鲜铁皮石斛茎、叶高效液相色谱特征图谱比较[J].广州中医药大学学报,2012(5):561-565.
[21]Solich P.Analysis of phenolic compounds by high performance liquid chromatography and ultra performance liquid chromatography[J].Talanta,2008,76(1):189-199.
[22]毛丽萍,李亚灵,温祥珍.苗期昼夜温差对番茄产量形成因子的影响分析[J].农业工程学报,2012(16):172-177.
[23]杨再强,朱凯,彭晓丹.昼夜温差对设施番茄叶片光合特性和叶绿素荧光参数的影响[J].生态学杂志,2013(12):3190-3196.
[24]Bachman G R,Mcmahon M J.Day and night temperature differential(DIF)or the absence of far-red light alters cell elongation in‘Celebrity White’petunia[J].Journal of the American Society for Horticultural Science,2006,131(3):309-312.
[25]Wang Y T.Effects of reversed day/night temperatures on a Doritaenopsis hybrid orchid[J].Hortscience,2004,39(4):834-834.
[26]杨再强,王学林,彭晓丹.人工环境昼夜温差对番茄营养物质和干物质分配的影响[J].农业工程学报,2014(5):138-147.
[27]Papadopoulos A P,Hao X M.Effects of day and night air temperature in early season on growth,productivity and energy use of spring tomato[J].Canadian Journal of Plant Science,2001,81(2):303-311.
[28]邱文伟,张光伦,张嵩.柑橘等果实糖代谢及其生态调控研究进展[J].四川农业大学学报,2005(1):114-119.
[29]陈艳秋,赵翔,刘昭霞.不同昼夜温差对设施番茄果实发育及产量的影响[J].北方园艺,2014(24):38-42.
[30]郑卫杰,郭子霞,王政.昼夜温差对文心兰试管苗生长的影响[J].西北林学院学报,2011,26(4):137-141.
[31]杨博,曹秀婷,王政.昼夜温差对非洲菊试管苗生长的影响[J].西北林学院学报,2012,27(2):88-92.
[32]艾娟,严宁,胡虹.温度对铁皮石斛生长及生理特性的影响[J].云南植物研究,2010,32(5):420-426.
[33]何铁光,苏江,王灿琴.铁皮石斛不同来源材料多糖和氨基酸含量的比较[J].广西农业科学,2007,38(1):32-34.
[34]Chen Z H,Chen Y L,Tao W U,et al.Study on structural characteristics and immunomodulatory activity of polysaccharide DMP4a-1 from Dendrobium moniliforme[J].Chinese Pharmaceutical Journal,2005,40(23):1781-1784.
[35]董思艳.不同叶面肥对铁皮石斛药效成分含量的影响[D].昆明:云南中医学院,2014.
[36]何铁光,杨丽涛,李杨瑞.铁皮石斛原球茎多糖DCPP3c-1的分离纯化及结构初步分析[J].分析测试学报,2008,27(2):143-147.
[37]诸燕,张爱莲,何伯伟.铁皮石斛总生物碱含量变异规律[J].中国中药杂志,2010,35(18):2388-2391.
[38]李燕,王春兰,王芳菲.铁皮石斛中的酚酸类及二氢黄酮类成分[J].中国药学杂志,2010,45(13):975-979.
[39]李燕,王春兰,王芳菲.铁皮石斛化学成分的研究[J].中国中药杂志,2010,35(13):1715-1719.
[40]王芳菲,李燕,董海玲,等.铁皮石斛中一个新化合物[J].中国药学杂志,2010,45(12):898-902.
[41]黄相中.一种提高铁皮石斛中多酚成分浸出率的方法:105560888 A[P].2016-05-11.
[42]郑洁,赵其阳,张耀海.超高效液相色谱法同时测定柑橘中主要酚酸和类黄酮物质[J].中国农业科学,2014,47(23):4706-4717.
[43]冉玥,焦必宁,赵其阳.超高效液相色谱法同时测定柑橘中11种类黄酮物质[J].食品科学,2013,34(4):168-172.
[44]周桂芬,陈素红,吕圭源.浙江省铁皮石斛黄酮类成分高效液相色谱指纹图谱的建立[J].中国药学杂志,2013,48(15):1261-1265.
[45]冯淑艳,董睿,彭黔荣,等.超高效液相色谱法测定贵州烟草中的游离氨基酸[J].化学研究与应用,2018,30(1):54-61.
[46]雷蓉,赵振霞,刘永利,等.超高效液相色谱法同时测定三七的叶和花中3个皂苷类成分含量[J].药物分析杂志,2018,38(7):1146-1151.
[47]白云,朱瑞超,李晋,等.超高效液相色谱法同时测定金花葵花中5个黄酮类成分含量[J].天津中医药,2018,35(2):147-150.
[48]孙存华,毛健民,白宝璋.昼夜变温促进小麦幼苗生长的酶学研究[J].吉林农业大学学报,2000,22(1):30-33.
[49]李莉,李佳,高青,等.昼夜温差对番茄生长发育、产量及果实品质的影响[J].应用生态学报,2015,26(9):2700-2706.
[50]郝敬虹,李天来,孟思达.夜间低温对薄皮甜瓜果实糖积累及代谢相关酶活性的影响[J].中国农业科学,2009,42(10):3592-3599.
[51]张明,夏鸿西,朱利泉.石斛组织培养研究进展[J].中国中药杂志,2000,25(6):3-6.
[52]潘宏兵,代沙,陈云清,等.铁皮石斛大棚种植环境调控技术[J].四川农业科技,2017(9):19-21.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |