超声波辅助提取高效液相色谱法测定菠萝叶中小麦黄素的含量
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摘要
研究超声波辅助提取菠萝叶中小麦黄素的最佳提取工艺和含量测定。利用单因素实验和正交实验,考察料液比、提取时间、提取温度、超声功率4个因素对提取工艺的的影响;采用高效液相色谱法对小麦黄素含量进行测定。菠萝叶中小麦黄素提取的最佳工艺为m(菠萝叶)∶V(乙酸乙酯)=1.0∶25(g·mL~(-1)),提取时间90min,超声提取温度75℃,超声功率192W,小麦黄素的平均收率可达0.494%(n=3)。当小麦黄素进样量在0.006～0.12 mg·mL~(-1)范围内与峰面积分值有良好的线性关系,r=0.99997,加样平均回收率为100.86%(n=5),相对标准偏差为3.24%。该方法简便有效,结果准确可靠。
Studying the optimum methodology of ultrasonic extracting tricin from the leaves of Ananas comosus and determining the content of tricin.By orthogonal experiment and single factor experiment,we investigated the effect of 4 factors containing ratio of solid to liquid,extraction time,extraction temperature,ultrasonic power.After the extraction,the content of tricin was measured by HPLC.The best extraction technology was m(the leaves of Ananas comosus):V(ethyl acetate)=1.0∶25(g·mL~(-1)),extraction time was 90 min,extraction temperature was 75℃,ultrasonic power was 192 W,theaverageyield of tricin was 0.494%(n=3),the amount of tricin had a excellent linear relationship with peak area when the amount of tricin ranged from 0.006～0.120 mg·mL~(-1),r=0.99997,average recovery rate was 100.86%(n=5),relative standard deviation was 3.24%.The method of extraction is feasible and effective,the results are accurate and reliable.
Studying the optimum methodology of ultrasonic extracting tricin from the leaves of Ananas comosus and determining the content of tricin.By orthogonal experiment and single factor experiment,we investigated the effect of 4 factors containing ratio of solid to liquid,extraction time,extraction temperature,ultrasonic power.After the extraction,the content of tricin was measured by HPLC.The best extraction technology was m(the leaves of Ananas comosus):V(ethyl acetate)=1.0∶25(g·mL~(-1)),extraction time was 90 min,extraction temperature was 75℃,ultrasonic power was 192 W,theaverageyield of tricin was 0.494%(n=3),the amount of tricin had a excellent linear relationship with peak area when the amount of tricin ranged from 0.006～0.120 mg·mL~(-1),r=0.99997,average recovery rate was 100.86%(n=5),relative standard deviation was 3.24%.The method of extraction is feasible and effective,the results are accurate and reliable.
引文
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[7]董南,王海燕,陈良龙,等.HPLC测定毛茛属几种植物中小麦黄素的含量[J].中国中药杂志,2007,32(22):2427-2428.
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[10]李存满,李兰芳,张勤增.高效液相色谱法测定苜蓿中苜蓿素的含量[J].药物分析杂志,2006,26(4):494-496.
[11]郭飞燕,王友志,王晓青,等.HPLC测定海南产胡椒中胡椒碱的含量[J].食品研究与开发,2012,33(12):74-76.
[2]王伟,丁怡,邢东明,等.菠萝叶酚类成分研究[J].中国中药杂志,2006,31(15):1242-1244.
[3]王金萍,王宏英,杜力军,等.菠萝叶中新的酰胺类成分[J].中国中药杂志,2007,32(5):401-403.
[4]国家中医药管理局中华本草编委会.中华本草,第 8 卷[M].上海:科学技术出版社,1999:7357.
[5]Manjir S K.Antibacterial activity,in vitro antioxidant activity and anthelmintic activity of ethanolic extract of Ananas comsus L.tender leaves[J].Pharmacologyonline,2010,(2):308-319.
[6]黄筱娟,陈文豪,纪明慧,等.菠萝叶的化学成分及生物活性研究[J].中草药,2015,46(7):949-951.
[7]董南,王海燕,陈良龙,等.HPLC测定毛茛属几种植物中小麦黄素的含量[J].中国中药杂志,2007,32(22):2427-2428.
[8]国家医药管理局中草药情报中心.植物药有效成分手册[M].北京:人民卫生出版社,1986.1073.
[9]李兰芳,张文彦,张魁.不同生长期白羊草中总黄酮及小麦黄素的含量测定[J].中国中药杂志,2000,25(5):272-274.
[10]李存满,李兰芳,张勤增.高效液相色谱法测定苜蓿中苜蓿素的含量[J].药物分析杂志,2006,26(4):494-496.
[11]郭飞燕,王友志,王晓青,等.HPLC测定海南产胡椒中胡椒碱的含量[J].食品研究与开发,2012,33(12):74-76.
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