2种杀虫剂对柑橘大实蝇的室内毒力测定
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摘要
柑橘大实蝇是为害柑橘类果实的毁灭性害虫。本研究采用饲喂法测定了丁硫克百威和阿维菌素对柑橘大实蝇雌成虫、雄成虫的毒力。结果表明,施药后24、48、72 h,丁硫克百威对雌成虫的LC50分别为93.06、45.43、7.04 mg/L,对雄成虫的LC50分别为91.09、27.41、6.78 mg/L;阿维菌素对雌成虫的LC50分别为34.44、5.04、2.22 mg/L,对雄成虫的LC50分别为56.71、7.57、3.37 mg/L。丁硫克百威和阿维菌素对柑橘大实蝇雌成虫和雄成虫均具有一定的毒力,且毒力可持续72 h,但雌成虫、雄成虫之间的毒力差异不明显;阿维菌素对柑橘大实蝇雌成虫、雄成虫的毒力高于丁硫克百威,但这种差异性随药后时间的延长而减小。本研究为柑橘大实蝇诱杀剂的开发和应用提供了一定的理论依据。
Bactrocera minax is a devastating pest that attacks citrus fruits.The toxicity bioassay of carbosulfan and abamectinto female and male adult Bactrocera minax was carried out using feeding method in the laboratory.The results showed that carbosulfan against adult Bactrocera minax with LC50 values of 93.06 mg/L in female and 91.09 mg/L in male after 24 h,45.43 mg/L in female and 27.41 mg/L in male after 48 h,7.04 mg/L in female and 6.78 mg/L in male after 72 h,respectively.While abamectin against adult Bactrocera minax with LC50 values of 34.44 mg/L in female and 56.71 mg/L in male after 24 h,5.04 mg/L in female and 7.57 mg/L in male after 48 h,2.22 mg/L in female and 3.37 mg/L in male after 72 h,respectively.Carbosulfan and abamectin had the extent toxicity with 72 h to adult Bactrocera minax,without difference between female and male.Abamectin had the higher toxicity than carbosulfan,and this difference decreased with the exposure time.The results provided a theoretical basis for the development and application of attractive toxicants.
Bactrocera minax is a devastating pest that attacks citrus fruits.The toxicity bioassay of carbosulfan and abamectinto female and male adult Bactrocera minax was carried out using feeding method in the laboratory.The results showed that carbosulfan against adult Bactrocera minax with LC50 values of 93.06 mg/L in female and 91.09 mg/L in male after 24 h,45.43 mg/L in female and 27.41 mg/L in male after 48 h,7.04 mg/L in female and 6.78 mg/L in male after 72 h,respectively.While abamectin against adult Bactrocera minax with LC50 values of 34.44 mg/L in female and 56.71 mg/L in male after 24 h,5.04 mg/L in female and 7.57 mg/L in male after 48 h,2.22 mg/L in female and 3.37 mg/L in male after 72 h,respectively.Carbosulfan and abamectin had the extent toxicity with 72 h to adult Bactrocera minax,without difference between female and male.Abamectin had the higher toxicity than carbosulfan,and this difference decreased with the exposure time.The results provided a theoretical basis for the development and application of attractive toxicants.
引文
[1]陈世骧,谢蕴贞.关于桔大实蝇的学名及其种征[J].昆虫学报,1955,5(1):123-126.
[2]DREW R A I,DORJI C,ROMING M C,et al.Attractiveness of various combinations of colors and shapes to females and males of Bactrocera minax(Diptera:Tephritidae)in a commercial mandarin grove in Bhutan[J].Journal of Economic Entomology,2006,99(5):1651-16516.
[3]肖伟,武可明,宫庆涛,等.柑橘大实蝇直肠粗提物的信息素功能[J].中国农业科学,2013,46(7):1501-1508.
[4]DORJI C,CLARKE A,DREW R,et al.Seasonal phenology of Bactrocera minax(Diptera:Tephritidae)in western Bhutan[J].Bulletin of Entomological Research,2006,96(5):531-538.
[5]黄秀琴,李正跃,李传仁,等.柑橘大实蝇成虫的翅载和额外负载能力[J].昆虫学报,2012,55(5):606-611.
[6]DONGY C,WANG Z J,CLARKE A R,et al.Pupal diapause development and termination is driven by low temperature chilling in Bactrocera minax[J].Journal of Pest Science,2013,86(3):429-436.
[7]刘路,周琼,宋傲群,等.柑橘大实蝇对不同柑橘品种的产卵偏好和幼虫取食选择[J].昆虫学报,2014,57(9):1037-1044.
[8]罗杰,桂连友,王福莲.初羽化的柑橘大实蝇成虫起飞日节律和降落位置[J].环境昆虫学报,2015,37(1):36-43.
[9]舒祥伦,肖志健.柑橘大实蝇的发生与防除[J].植物医生,2006,19(3):26.
[10]曾卓华,刘洪,肖国民,等.柑橘大实蝇防控技术的新突破[J].植物检疫,2011,25(5):36-39.
[11]范京安.四川柑桔大实蝇发生与防治[J].植物检疫,2002,16(3):150-152.
[12]冉峰,袁文斌,张亚东.应用几种诱剂监测柑橘大实蝇成虫发生期及防治适期初报[J].浙江柑桔,2011,28(2):29-31.
[13]王金涛,董永成,李宗锴,等.昆虫不育技术防治柑橘大实蝇研究概况[J].应用昆虫学报,2013,50(3):848-852.
[14]张桂芬,王福莲,吕志创,等.柑橘大实蝇生物、生态学及辐照不育技术最新研究进展[J].生物安全学报,2015,24(2):171-176.
[15]杨鸿雁,周莉萍.不同诱杀剂配方对柑橘大实蝇的诱杀效果[J].农技服务,2011,28(1):48.
[16]刘雄.桔丰和果瑞特防治柑橘大实蝇的效果研究[J].湖南农业科学,2011(16):21.
[17]周斌,张学平.果瑞特防治柑橘大实蝇试验效果研究[J].农业与技术,2016,36(1):34.
[18]张敬存,韩奎珍.丁硫克百威的高效液相色谱分析[J].农药,2000,39(5):25.
[19]刘浩强,李鸿筠,胡军华,等.桔小实蝇诱捕器设置及常用杀虫剂筛选[J].中国南方果树,2009,38(1):56-57.
[20]曾益良,王大生,秦小薇,等.几种氨基甲酸酯类杀虫剂对美洲斑潜蝇的防治效果[J].昆虫知识,2003,40(5):436-439.
[21]慕卫,丁中,何茂华,等.韭菜迟眼蕈蚊的生测方法及防治药剂研究[J].华北农学报,2002,17(增刊1):12-16.
[22]MACCONNELL J G,DEMCHAK R J,PREISER F A,et al.Relative stability,toxicity,and penetrability of abamectin and its 8,9-oxide[J].Journal of Agricultural and Food Chemistry,1989,37:1498-1501.
[23]BLOOMQUIST J R.Toxicology,mode of action and target site-mediated resistance to insecticides acting on chloride channels[J].Comparative Biochemistry and Physiology,1993,106C(2):301-314.
[24]侯宏伟.生物农药阿维菌素的制剂研究[D].上海:上海师范大学,2012.
[25]唐启义.DPS数据处理系统:实验设计统计分析及数据挖掘[M].北京:科学出版社,2010.
[26]赵菊鹏,田世尧,曾杨,等.几种杀虫剂对桔小实蝇成虫的毒力测定[J].植物检疫,2001,15(6):342-343.
[27]丁正民,张典铨.吡虫啉和丁硫克百威混用对柑桔蚜虫的防效[J].东南园艺,2004(3):33-34.
[28]印家厚.高效广谱杀虫剂好年冬[J].湖北植保,1999(3):37.
[29]白文星,秦克辉,张素英.“好年冬”防治柑桔潜叶蛾,蚜虫和锈壁虱试验[J].南方园艺,1995(2):10-13.
[30]邓明学,谭有龙,唐美欣,等.联苯菊酯等6种农药对柑桔木虱成虫室内毒力测定及田间药效试验[J].中国农学通报,2009,25(15):169-173.
[31]刘开林,何林,王进军,等.害虫及害螨对阿维菌素抗药性研究进展[J].昆虫知识,2007,44(2):194-200.
[2]DREW R A I,DORJI C,ROMING M C,et al.Attractiveness of various combinations of colors and shapes to females and males of Bactrocera minax(Diptera:Tephritidae)in a commercial mandarin grove in Bhutan[J].Journal of Economic Entomology,2006,99(5):1651-16516.
[3]肖伟,武可明,宫庆涛,等.柑橘大实蝇直肠粗提物的信息素功能[J].中国农业科学,2013,46(7):1501-1508.
[4]DORJI C,CLARKE A,DREW R,et al.Seasonal phenology of Bactrocera minax(Diptera:Tephritidae)in western Bhutan[J].Bulletin of Entomological Research,2006,96(5):531-538.
[5]黄秀琴,李正跃,李传仁,等.柑橘大实蝇成虫的翅载和额外负载能力[J].昆虫学报,2012,55(5):606-611.
[6]DONGY C,WANG Z J,CLARKE A R,et al.Pupal diapause development and termination is driven by low temperature chilling in Bactrocera minax[J].Journal of Pest Science,2013,86(3):429-436.
[7]刘路,周琼,宋傲群,等.柑橘大实蝇对不同柑橘品种的产卵偏好和幼虫取食选择[J].昆虫学报,2014,57(9):1037-1044.
[8]罗杰,桂连友,王福莲.初羽化的柑橘大实蝇成虫起飞日节律和降落位置[J].环境昆虫学报,2015,37(1):36-43.
[9]舒祥伦,肖志健.柑橘大实蝇的发生与防除[J].植物医生,2006,19(3):26.
[10]曾卓华,刘洪,肖国民,等.柑橘大实蝇防控技术的新突破[J].植物检疫,2011,25(5):36-39.
[11]范京安.四川柑桔大实蝇发生与防治[J].植物检疫,2002,16(3):150-152.
[12]冉峰,袁文斌,张亚东.应用几种诱剂监测柑橘大实蝇成虫发生期及防治适期初报[J].浙江柑桔,2011,28(2):29-31.
[13]王金涛,董永成,李宗锴,等.昆虫不育技术防治柑橘大实蝇研究概况[J].应用昆虫学报,2013,50(3):848-852.
[14]张桂芬,王福莲,吕志创,等.柑橘大实蝇生物、生态学及辐照不育技术最新研究进展[J].生物安全学报,2015,24(2):171-176.
[15]杨鸿雁,周莉萍.不同诱杀剂配方对柑橘大实蝇的诱杀效果[J].农技服务,2011,28(1):48.
[16]刘雄.桔丰和果瑞特防治柑橘大实蝇的效果研究[J].湖南农业科学,2011(16):21.
[17]周斌,张学平.果瑞特防治柑橘大实蝇试验效果研究[J].农业与技术,2016,36(1):34.
[18]张敬存,韩奎珍.丁硫克百威的高效液相色谱分析[J].农药,2000,39(5):25.
[19]刘浩强,李鸿筠,胡军华,等.桔小实蝇诱捕器设置及常用杀虫剂筛选[J].中国南方果树,2009,38(1):56-57.
[20]曾益良,王大生,秦小薇,等.几种氨基甲酸酯类杀虫剂对美洲斑潜蝇的防治效果[J].昆虫知识,2003,40(5):436-439.
[21]慕卫,丁中,何茂华,等.韭菜迟眼蕈蚊的生测方法及防治药剂研究[J].华北农学报,2002,17(增刊1):12-16.
[22]MACCONNELL J G,DEMCHAK R J,PREISER F A,et al.Relative stability,toxicity,and penetrability of abamectin and its 8,9-oxide[J].Journal of Agricultural and Food Chemistry,1989,37:1498-1501.
[23]BLOOMQUIST J R.Toxicology,mode of action and target site-mediated resistance to insecticides acting on chloride channels[J].Comparative Biochemistry and Physiology,1993,106C(2):301-314.
[24]侯宏伟.生物农药阿维菌素的制剂研究[D].上海:上海师范大学,2012.
[25]唐启义.DPS数据处理系统:实验设计统计分析及数据挖掘[M].北京:科学出版社,2010.
[26]赵菊鹏,田世尧,曾杨,等.几种杀虫剂对桔小实蝇成虫的毒力测定[J].植物检疫,2001,15(6):342-343.
[27]丁正民,张典铨.吡虫啉和丁硫克百威混用对柑桔蚜虫的防效[J].东南园艺,2004(3):33-34.
[28]印家厚.高效广谱杀虫剂好年冬[J].湖北植保,1999(3):37.
[29]白文星,秦克辉,张素英.“好年冬”防治柑桔潜叶蛾,蚜虫和锈壁虱试验[J].南方园艺,1995(2):10-13.
[30]邓明学,谭有龙,唐美欣,等.联苯菊酯等6种农药对柑桔木虱成虫室内毒力测定及田间药效试验[J].中国农学通报,2009,25(15):169-173.
[31]刘开林,何林,王进军,等.害虫及害螨对阿维菌素抗药性研究进展[J].昆虫知识,2007,44(2):194-200.