高盐突变对凡纳滨对虾(Litopenaeus vannamei)生长性能及相关酶活力的影响
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摘要
为模拟夏季水分蒸发水体盐度快速升高对凡纳滨对虾(Litopenaeusvannamei)生长性能及理化调节的影响,试验设置盐度从30突变至35、40、45、50、55及60,以盐度30为对照,突变盐度下养殖28d,每7d检测凡纳滨对虾的存活率、相对增重率、体长增长率,试验结束时检测血清Na+/K+-ATP酶、总ATP酶、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)和超氧化物歧化酶(SOD)活力。结果表明,高盐突变显著抑制凡纳滨对虾的存活率和相对增重率(P<0.05),随着突变盐度的升高,凡纳滨对虾的相对增重率逐渐降低,60盐度组仅为对照组的15.53%。盐度突变至50后,凡纳滨对虾存活率显著下降。随着高盐突变幅度的增加,Na+/K+-ATP酶和ACP酶活力受到显著影响(P<0.05),其中Na+/K+-ATP酶活力逐渐上升,在突变60盐度时表现为最高;ACP酶表现为先上升再下降的单峰变化趋势。总ATP酶、SOD酶、AKP酶受影响不显著(P>0.05)。结果表明,高盐突变幅度越大,凡纳滨对虾存活率越低、生长越缓慢,Na+/K+-ATP酶活力升高,渗透调节能力增强,ACP酶活力升高,说明高盐突变激发凡纳滨对虾机体代谢活力。
To investigate the effect of abrupt high-salinity change on growth,survival rate and enzyme activity of Litopenaeus vannamei in a 28-day experiment in which salinity changed abruptly from 30(the control) to 35,40,45,50,55,and 60 in the first 12 h.The survival rate,relative weight gain,and growth rate were determined every 7 days,and the acvtivities of Na+/K+-ATPase,T-ATPase,alkaline phosphatase(AKP),acidic phosphatase(ACP),and superoxide dismutase(SOD) were detected at the end of experiment.The results show that the high-salinity abrupt change inhibited the survival and relative weight gain of the shrimp significantly.The relative weight gain was reduced gradually with the increase of salinity,which was only 15.53% in 60 salinity group of the control.After the salinity changed abruptly to 50,the survival rate was significantly reduced.On the other hand,with the sudden increase of salinity,the activities of Na+/K+-ATPase and ACP were significantly(P<0.05) affected,Na+/K+-ATPase reached the highest at the salinity of 60,and the ACP showed a single-peak trend.However,the activities of T-ATPase,SOD,and AKP were not significantly affected(P>0.05).Therefore,the greater the range of the change,the lower of the survival rate and growth rate;and the activities of Na+/K+-ATPase,ACP,and osmotic regulation capacity increased.An abrupt salinity change could stimulate the metabolic activity of L.vannamei.
To investigate the effect of abrupt high-salinity change on growth,survival rate and enzyme activity of Litopenaeus vannamei in a 28-day experiment in which salinity changed abruptly from 30(the control) to 35,40,45,50,55,and 60 in the first 12 h.The survival rate,relative weight gain,and growth rate were determined every 7 days,and the acvtivities of Na+/K+-ATPase,T-ATPase,alkaline phosphatase(AKP),acidic phosphatase(ACP),and superoxide dismutase(SOD) were detected at the end of experiment.The results show that the high-salinity abrupt change inhibited the survival and relative weight gain of the shrimp significantly.The relative weight gain was reduced gradually with the increase of salinity,which was only 15.53% in 60 salinity group of the control.After the salinity changed abruptly to 50,the survival rate was significantly reduced.On the other hand,with the sudden increase of salinity,the activities of Na+/K+-ATPase and ACP were significantly(P<0.05) affected,Na+/K+-ATPase reached the highest at the salinity of 60,and the ACP showed a single-peak trend.However,the activities of T-ATPase,SOD,and AKP were not significantly affected(P>0.05).Therefore,the greater the range of the change,the lower of the survival rate and growth rate;and the activities of Na+/K+-ATPase,ACP,and osmotic regulation capacity increased.An abrupt salinity change could stimulate the metabolic activity of L.vannamei.
引文
马英杰,张志峰,马爱军等,1999.低盐度突变对中国对虾仔虾存活率的影响.海洋与湖沼,30(2):134-138
王兴强,马甡,董双林,2004.凡纳滨对虾生物学及养殖生态学研究进展.海洋湖沼通报,(4):94-100
孔杰,栾生,罗坤等,2017.不同盐度下凡纳滨对虾生长和存活性状遗传评估.水产学报,41(4):573-579
申玉春,陈作洲,刘丽等,2012.盐度和营养对凡纳滨对虾蜕壳和生长的影响.水产学报,36(2):290-299
刘存岐,王安利,王维娜等,2001.海水中几种金属离子对中国对虾幼体体内碱性磷酸酶和ATPase的影响.水产学报,25(4):298-303
刘慧杰,潘鲁青,胡发文,2008.凡纳滨对虾在盐度变化下免疫机能评价的研究.海洋湖沼通报,(2):159-166
许燕,杨洁,孙静秋等,2010.凡纳滨对虾不同组织内SOD、POD酶的细胞化学定位.水生生物学报,34(2):402-409
李娜,王仁杰,赵玉超等,2017.高盐胁迫对凡纳滨对虾生长指标、血浆渗透压及Na+-K+-ATP酶活力的影响.浙江海洋学院学报(自然科学版),36(3):196-201
李娜,赵玉超,王仁杰等,2018.高盐胁迫对凡纳滨对虾消化及免疫相关酶活力的影响.生态学报,38(4):1411-1417
李二超,陈立侨,曾嶒等,2009.盐度对凡纳滨对虾体组织蛋白质积累、氨基酸组成和转氨酶活性的影响.水生生物学报,33(3):532-538
李玉全,李永生,赵法箴,2015.盐度渐变与骤变对脊尾白虾渗透、代谢及免疫相关酶活力的影响.生态学报,35(21):7229-7235
张丹,王芳,董双林,2016.周期性盐度波动对凡纳滨对虾游离氨基酸含量及渗透调节相关基因表达的影响.中国水产科学,23(5):1130-1136
赵磊,龙晓文,吴旭干等,2016.水体盐度对中华绒螯蟹成体雄蟹渗透压调节和生理代谢的影响.水生生物学报,40(1):27-34
栾生,罗坤,阮晓红等,2013.凡纳滨对虾(Litopenaeus vannamei)体重、存活性状的遗传参数和基因型与环境互作效应.海洋与湖沼,44(2):445-452
戴习林,张立田,臧维玲等,2012.Ca2+、Mg2+、盐度对凡纳滨对虾存活、生长及风味的影响.水产学报,36(6):914-921
Bray W A,Lawrence A L,Leung-Trujillo J R,1994.The effect of salinity on growth and survival of Penaeus vannamei,with observations on the interaction of IHHN virus and salinity.Aquaculture,122(2-3):133-146
Cheng W,Chu C J,2000.Effects of pH,temperature and salinity on immune parameters of the freshwater prawn Macrobrachium rosenbergii.Fish&Shellfish Immunology,10(4):387-391
Dalla Via G J,1986.Salinity responses of the juvenile penaeid shrimp Penaeus japonicus:Ⅰ.Oxygen consumption and estimations of productivity.Aquaculture,55(4):297-306
Li W,Yin D,Zhang A et al,2002.Toxicity of chloroanilines and effects on superoxide dismutase activities in serum of crucian carp(Carassius auratus).Bulletin of Environmental Contamination and Toxicology,68(5):630-636
Mancera J M,McCormick S D,2000.Rapid activation of gill Na+,K+-ATPase in the euryhaline teleost Fundulus heteroclitus.Journal of Experimental Zoology,287(4):263-274
Menz A,Blake B F,1980.Experiments on the growth of Penaeus vannamei Boone.Journal of Experimental Marine Biology and Ecology,48(2):99-111
Perazzolo L M,Gargioni R,Ogliari P et al,2002.Evaluation of some hemato-immunological parameters in the shrimp Farfantepenaeus paulensis submitted to environmental and physiological stress.Aquaculture,214(1-4):19-33
Ponce-Palafox J,Martinez-Palacios C A,Ross L G,1997.The effects of salinity and temperature on the growth and survival rates of juvenile white shrimp,Penaeus vannamei,Boone,1931.Aquaculture,157(1-2):107-115
Stern S,Daniels H,Leteller E,1990.Tolerance of post larvae and juvenile Penaeus vannamei to low salinity.In:Abstracts,World Aquaculture 90.Halifax,Nova Scotia,Canada,T30.12.Ottawa,Ont.,Canada:National Research Council Canada
Towle D W,1981.Role of Na++K+-ATPase in ionic regulation by marine and estuarine animals.Marine Biology Letters,2:107-121
Yang W K,Hseu J R,Tang C H et al,2009.Na+/K+-ATPase expression in gills of the euryhaline sailfin molly,Poecilia latipinna,is altered in response to salinity challenge.Journal of Experimental Marine Biology and Ecology,375(1-2):41-50
Yokota Y,Nakano E,1982.Comparative studies on particulate acid phosphatases in sea urchin eggs.Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,71(4):563-567
Yun Y S,Lee Y N,2004.Purification and some properties of superoxide dismutase from Deinococcus radiophilus,the UV-resistant bacterium.Extremophiles,8(3):237-242
王兴强,马甡,董双林,2004.凡纳滨对虾生物学及养殖生态学研究进展.海洋湖沼通报,(4):94-100
孔杰,栾生,罗坤等,2017.不同盐度下凡纳滨对虾生长和存活性状遗传评估.水产学报,41(4):573-579
申玉春,陈作洲,刘丽等,2012.盐度和营养对凡纳滨对虾蜕壳和生长的影响.水产学报,36(2):290-299
刘存岐,王安利,王维娜等,2001.海水中几种金属离子对中国对虾幼体体内碱性磷酸酶和ATPase的影响.水产学报,25(4):298-303
刘慧杰,潘鲁青,胡发文,2008.凡纳滨对虾在盐度变化下免疫机能评价的研究.海洋湖沼通报,(2):159-166
许燕,杨洁,孙静秋等,2010.凡纳滨对虾不同组织内SOD、POD酶的细胞化学定位.水生生物学报,34(2):402-409
李娜,王仁杰,赵玉超等,2017.高盐胁迫对凡纳滨对虾生长指标、血浆渗透压及Na+-K+-ATP酶活力的影响.浙江海洋学院学报(自然科学版),36(3):196-201
李娜,赵玉超,王仁杰等,2018.高盐胁迫对凡纳滨对虾消化及免疫相关酶活力的影响.生态学报,38(4):1411-1417
李二超,陈立侨,曾嶒等,2009.盐度对凡纳滨对虾体组织蛋白质积累、氨基酸组成和转氨酶活性的影响.水生生物学报,33(3):532-538
李玉全,李永生,赵法箴,2015.盐度渐变与骤变对脊尾白虾渗透、代谢及免疫相关酶活力的影响.生态学报,35(21):7229-7235
张丹,王芳,董双林,2016.周期性盐度波动对凡纳滨对虾游离氨基酸含量及渗透调节相关基因表达的影响.中国水产科学,23(5):1130-1136
赵磊,龙晓文,吴旭干等,2016.水体盐度对中华绒螯蟹成体雄蟹渗透压调节和生理代谢的影响.水生生物学报,40(1):27-34
栾生,罗坤,阮晓红等,2013.凡纳滨对虾(Litopenaeus vannamei)体重、存活性状的遗传参数和基因型与环境互作效应.海洋与湖沼,44(2):445-452
戴习林,张立田,臧维玲等,2012.Ca2+、Mg2+、盐度对凡纳滨对虾存活、生长及风味的影响.水产学报,36(6):914-921
Bray W A,Lawrence A L,Leung-Trujillo J R,1994.The effect of salinity on growth and survival of Penaeus vannamei,with observations on the interaction of IHHN virus and salinity.Aquaculture,122(2-3):133-146
Cheng W,Chu C J,2000.Effects of pH,temperature and salinity on immune parameters of the freshwater prawn Macrobrachium rosenbergii.Fish&Shellfish Immunology,10(4):387-391
Dalla Via G J,1986.Salinity responses of the juvenile penaeid shrimp Penaeus japonicus:Ⅰ.Oxygen consumption and estimations of productivity.Aquaculture,55(4):297-306
Li W,Yin D,Zhang A et al,2002.Toxicity of chloroanilines and effects on superoxide dismutase activities in serum of crucian carp(Carassius auratus).Bulletin of Environmental Contamination and Toxicology,68(5):630-636
Mancera J M,McCormick S D,2000.Rapid activation of gill Na+,K+-ATPase in the euryhaline teleost Fundulus heteroclitus.Journal of Experimental Zoology,287(4):263-274
Menz A,Blake B F,1980.Experiments on the growth of Penaeus vannamei Boone.Journal of Experimental Marine Biology and Ecology,48(2):99-111
Perazzolo L M,Gargioni R,Ogliari P et al,2002.Evaluation of some hemato-immunological parameters in the shrimp Farfantepenaeus paulensis submitted to environmental and physiological stress.Aquaculture,214(1-4):19-33
Ponce-Palafox J,Martinez-Palacios C A,Ross L G,1997.The effects of salinity and temperature on the growth and survival rates of juvenile white shrimp,Penaeus vannamei,Boone,1931.Aquaculture,157(1-2):107-115
Stern S,Daniels H,Leteller E,1990.Tolerance of post larvae and juvenile Penaeus vannamei to low salinity.In:Abstracts,World Aquaculture 90.Halifax,Nova Scotia,Canada,T30.12.Ottawa,Ont.,Canada:National Research Council Canada
Towle D W,1981.Role of Na++K+-ATPase in ionic regulation by marine and estuarine animals.Marine Biology Letters,2:107-121
Yang W K,Hseu J R,Tang C H et al,2009.Na+/K+-ATPase expression in gills of the euryhaline sailfin molly,Poecilia latipinna,is altered in response to salinity challenge.Journal of Experimental Marine Biology and Ecology,375(1-2):41-50
Yokota Y,Nakano E,1982.Comparative studies on particulate acid phosphatases in sea urchin eggs.Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,71(4):563-567
Yun Y S,Lee Y N,2004.Purification and some properties of superoxide dismutase from Deinococcus radiophilus,the UV-resistant bacterium.Extremophiles,8(3):237-242
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