马氏珠母贝金黄壳色选育群体与养殖群体血清免疫酶活力比较
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摘要
为了给金黄壳色选育群体与性状评价提供参考,本研究从金黄壳色选育群体与对照养殖群体取样进行植核育珠,植核手术后第7天与第30天,比较2个群体成活率与留核率差异,同时比较了植核手术后第7天2个群体血清免疫酶活力差异。结果表明:植核后第7天与第30天,金黄壳色选育群体的留核率与成活率显著大于养殖群体(P<0.05);植核前与植核后第7天,金黄壳色选育群体碱性磷酸酶、酸性磷酸酶、溶菌酶、亮氨酸氨基肽酶、超氧化物歧化酶、谷胱甘肽过氧化氢酶、酚氧化酶显著大于养殖群体(P<0.05);金黄壳色选育群体的过氧化氢酶活力大于养殖群体,但2个群体的差异不显著(P>0.05);金黄壳色选育群体的丙二醛的含量均小于养殖群体。因此休养期间金黄壳色选育群体的育珠性状优于大于对照养殖群体。
To provide references for yellow colored line selection and trait evaluation, the yellow colored line and the cultured stock were subjected for nucleation in this study. On the 7 thday and 30 thday after the nucleus insertion operation, the retention rate and survival rate of the two groups were compared. The differences of serum immunase activity between the two groups on the 7 thday after nucleus insertion operation were also evaluated. The results showed that the retention rate and survival rate of yellow colored line were significantly greater than those of the cultured stock on the 7 thday and 30 thday after nucleation operation(P < 0.05). The yellow colored line had significantly higher alkaline phosphatase, acid phosphatase, lysozyme, leucine arylamidase, superoxide dismutase, and glutathione peroxidase and monophenol oxidase than the cultured stock before nucleus insertion operation and on the 7 thday after nucleus insertion operation(P < 0.05). The yellow colored line had higher catalase activity than the cultured stock before nucleus insertion operation and on the 7 thday after nucleus insertion operation. However, the differences between the stocks were not significant(P > 0.05). The yellow colored line had smaller malondialdehyde content than the cultured stock.The pearl production traits of the yellow colored line during the recuperation period after nucleus insertion operation are prior to those of the cultured stock.
To provide references for yellow colored line selection and trait evaluation, the yellow colored line and the cultured stock were subjected for nucleation in this study. On the 7 thday and 30 thday after the nucleus insertion operation, the retention rate and survival rate of the two groups were compared. The differences of serum immunase activity between the two groups on the 7 thday after nucleus insertion operation were also evaluated. The results showed that the retention rate and survival rate of yellow colored line were significantly greater than those of the cultured stock on the 7 thday and 30 thday after nucleation operation(P < 0.05). The yellow colored line had significantly higher alkaline phosphatase, acid phosphatase, lysozyme, leucine arylamidase, superoxide dismutase, and glutathione peroxidase and monophenol oxidase than the cultured stock before nucleus insertion operation and on the 7 thday after nucleus insertion operation(P < 0.05). The yellow colored line had higher catalase activity than the cultured stock before nucleus insertion operation and on the 7 thday after nucleus insertion operation. However, the differences between the stocks were not significant(P > 0.05). The yellow colored line had smaller malondialdehyde content than the cultured stock.The pearl production traits of the yellow colored line during the recuperation period after nucleus insertion operation are prior to those of the cultured stock.
引文
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[12]张跃环,闫喜武,张澎,等.贝类壳色多态的研究概况及展望[J].水产科学,2008,27(12):680-683.
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[16]邓岳文,张善发,符韶,等.马氏珠母贝黄壳色选系F1和养殖群体形态性状比较[J].广东洋大学学报,2007,27(6):77-79.
[17]王庆恒,邓岳文,杜晓东,等.马氏珠母贝4个壳色选系F1幼虫的生长比较[J].中国水产科学,2008,15(3):488-492.
[18]王庆恒,张善发,杜晓东,等.马氏珠母贝黄壳色选系F1与对照组耗氧率和排氨率的比较[J].水产学报,2009,33(5):790-796.
[19]符韶,谢绍河,邓岳文,等.利用马氏珠母贝4个壳色系F3培育厚层优质珍珠[J].水产学报,2012,36(9):1418-1424.
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[22]郑怀平,张国范,刘晓,等.不同贝壳颜色海湾扇贝家系的建立及生长发育的研究[J].海洋与湖沼,2003,36(6):632-639.
[23]刘晓,张国范,赵洪恩.皱纹盘鲍“中国红”品系的选育[J].动物学杂志,2003,38(4):27.
[24]刘晓,高其康,张国范.不同壳色皱纹盘鲍杂交家系J1RHF1全长cDNA文库的构建[J].水产学报,2004,28(1):23-28.
[25]闫喜武,张国范,杨凤,等.菲律宾蛤仔莆田群体两个壳色品系生长发育的比较[J].大连水产学院学报,2005,20(4):266-269.
[26]闫喜武,张国范,杨凤,等.菲律宾蛤仔莆田群体与大连群体生物学比较[J].生态学报,2005,25(12):3329-3334.
[27]周畅,谢莉萍,张荣庆.插核手术对合浦珠母贝免疫水平的影响[J].水产学报,2001,25(5):419-423.
[28]焦钰,杜晓东,师尚丽,等.珍珠囊发育中马氏珠母贝血清免疫因子的变化规律[J].广东海洋大学学报,2007,27(4):21-24.
[29]丁鉴锋,杨霏,闫喜武,等.不同壳色菲律宾蛤仔免疫机能的比较研究[J].大连海洋大学学报,2012,27(5):411-416.
[30]郝振林,刘京哲,唐雪娇,等.高温下3种壳色虾夷扇贝存活率、代谢率、免疫酶活力及HSP70表达的比较研究[J].海洋科学,2015,39(11):108-115.
[2]邓陈茂,童银洪,符韶.马氏珠母贝的研究进展[J].现代农业科技,2009,2:204-206.
[3]杜晓东,邓岳文,王庆恒,等.马氏珠母贝海选1号[J].中国水产,2015,10:53-56.
[4]Brink D J, van der Berg N G, Botha A J. Iridescent colors on seashells:an optical and structural investigation of Helcion pruinosus[J]. Applied Optics, 2002, 1:717-722.
[5]Liu X, Deng Y W, Zhang G F. Growth of eight Pacific abalone families at three temperatures[J]. Acta Oceanologica Sinica, 2005,24:148-153.
[6]闫喜武,张国范,杨凤,等.菲律宾蛤仔莆田群体两个壳色品系生长发育的比较[J].大连水产学院学报,2005,20(4):266-269.
[7]Zheng H P, Zhang G F, Liu X. Comparison of growth and survival of larvae among different shell color stocks of bay scallop Argopecten irradians irradians[J].Chinese Journal of Oceanology and Limnology, 2005, 23(2):183-188.
[8]Liu X, Deng Y W, Zhang G F. Growth of eight Pacific abalone families at three temperatures[J]. Acta Oceanologica Sinica, 2005,24:148-153.
[9]顾向飞.文蛤不同壳色花纹品系生长性状和营养成分的差异分析[D].宁波:宁波大学, 2014.
[10]Zheng H P, Zhang G F, Liu X. Comparison of growth and survival of larvae among different shell color stocks of bay scallop Argopecten irradians irradians[J].Chinese Journal of Oceanology and Limnology, 2005, 23(2):183-188.
[11]管云雁,何毛贤.海产经济贝类壳色多态性的研究进展[J].海洋通报,2009,28(1):108-114.
[12]张跃环,闫喜武,张澎,等.贝类壳色多态的研究概况及展望[J].水产科学,2008,27(12):680-683.
[13]何毛贤.马氏珠母贝红壳品系“南科珍珠红”的培育[J].热带海洋学报,2006,25(1):58.
[14]张红玉,何毛贤,管云雁.马氏珠母贝红色壳家系不同世代遗传变异的SRAP分析[J].水产学报,2009,33(5):727-733
[15]Wada K T, Komaru A. Color and weight of pearls produced by grafting the mantle tissue from a selected population for white shell coloration of the Japanese pearl oyster, Pinctada fucata martensii(Dunker)[J]. Aquaculture, 1996,142:25-32.
[16]邓岳文,张善发,符韶,等.马氏珠母贝黄壳色选系F1和养殖群体形态性状比较[J].广东洋大学学报,2007,27(6):77-79.
[17]王庆恒,邓岳文,杜晓东,等.马氏珠母贝4个壳色选系F1幼虫的生长比较[J].中国水产科学,2008,15(3):488-492.
[18]王庆恒,张善发,杜晓东,等.马氏珠母贝黄壳色选系F1与对照组耗氧率和排氨率的比较[J].水产学报,2009,33(5):790-796.
[19]符韶,谢绍河,邓岳文,等.利用马氏珠母贝4个壳色系F3培育厚层优质珍珠[J].水产学报,2012,36(9):1418-1424.
[20]Deng Y W, Fu S, Lu Y Z, et al. Fertilization, hatching, survival, and growth of third-generation colored pearl oyster Pinctada martensii stocks[J]. Journal of Applied Aquaculture, 25:2, 113-120.
[21]Newkirk G F. Genetics of shell color in Mytilus edulis L. and the association of growth rate with shell color[J]. Journal of Experimental Marine Biology and Ecology, 1980, 47(1):89-94.
[22]郑怀平,张国范,刘晓,等.不同贝壳颜色海湾扇贝家系的建立及生长发育的研究[J].海洋与湖沼,2003,36(6):632-639.
[23]刘晓,张国范,赵洪恩.皱纹盘鲍“中国红”品系的选育[J].动物学杂志,2003,38(4):27.
[24]刘晓,高其康,张国范.不同壳色皱纹盘鲍杂交家系J1RHF1全长cDNA文库的构建[J].水产学报,2004,28(1):23-28.
[25]闫喜武,张国范,杨凤,等.菲律宾蛤仔莆田群体两个壳色品系生长发育的比较[J].大连水产学院学报,2005,20(4):266-269.
[26]闫喜武,张国范,杨凤,等.菲律宾蛤仔莆田群体与大连群体生物学比较[J].生态学报,2005,25(12):3329-3334.
[27]周畅,谢莉萍,张荣庆.插核手术对合浦珠母贝免疫水平的影响[J].水产学报,2001,25(5):419-423.
[28]焦钰,杜晓东,师尚丽,等.珍珠囊发育中马氏珠母贝血清免疫因子的变化规律[J].广东海洋大学学报,2007,27(4):21-24.
[29]丁鉴锋,杨霏,闫喜武,等.不同壳色菲律宾蛤仔免疫机能的比较研究[J].大连海洋大学学报,2012,27(5):411-416.
[30]郝振林,刘京哲,唐雪娇,等.高温下3种壳色虾夷扇贝存活率、代谢率、免疫酶活力及HSP70表达的比较研究[J].海洋科学,2015,39(11):108-115.
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