表达HIV-1 CRF01＿AEgag的rAd5疫苗构建及其免疫原性评价
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摘要
目的构建表达HIV-1 CRF01_AE gag基因的重组腺病毒(rAd5)载体疫苗,并在小鼠体内评价其免疫效果。方法利用实验室前期改造优化的HIV-1 CRF01_AE gag基因,构建获得携带有gag基因的重组Ad5疫苗,即rAd5-HIV AEgag。经鉴定证实重组Ad5疫苗可以表达Gag蛋白后,在BALB/c小鼠体内评价重组疫苗rAd5-HIV AEgag的免疫效果。结果重组腺病毒疫苗可以准确地表达Gag蛋白,疫苗免疫BALB/c小鼠可以诱导出较高强度的体液和细胞免疫应答反应。结论成功构建了表达HIV-1 CRF01_AE gag基因的重组Ad5疫苗。
Objective To construct recombinant Ad5 vaccine encoding codon-optimazed HIV-1 CRF01_AE gag gene and evaluate its immunogenicity in mice. Methods The HIV-1 CRF01_AE gag gene was optimized in the previous phase of our laboratory,a recombinant Ad5 vaccine carrying the gag gene,rAd5-HIV AEgag,was constructed. After confirmed that the recombinant Ad5 vaccine could express Gag protein,the immune effect of recombinant vaccine rAd5-HIVAEgag was evaluated in BALB/c mice.Results Recombinant Ad5 vaccine expressed Gag protein efficiently and induced high level of humoral and cellular immune responses in immunized BALB/c mice. Conclusion The recombinant Ad5 vaccine encoding HIV-1 CRF01 _AE gag gene was constructed successfully.
Objective To construct recombinant Ad5 vaccine encoding codon-optimazed HIV-1 CRF01_AE gag gene and evaluate its immunogenicity in mice. Methods The HIV-1 CRF01_AE gag gene was optimized in the previous phase of our laboratory,a recombinant Ad5 vaccine carrying the gag gene,rAd5-HIV AEgag,was constructed. After confirmed that the recombinant Ad5 vaccine could express Gag protein,the immune effect of recombinant vaccine rAd5-HIVAEgag was evaluated in BALB/c mice.Results Recombinant Ad5 vaccine expressed Gag protein efficiently and induced high level of humoral and cellular immune responses in immunized BALB/c mice. Conclusion The recombinant Ad5 vaccine encoding HIV-1 CRF01 _AE gag gene was constructed successfully.
引文
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[2]Williams WB,Liao HX,Moody MA,et al.HIV-1VACCINES.Diversion of HIV-1 vaccine-inducedimmunity by gp41-microbiota cross-reactive antibodies[J].Science,2015,349(6249):1253.
[3]Ferrando-Martinez S,Casazza JP,Leal M,et al.Differential Gag-specific polyfunctional T cell maturation patterns in HIV-1elite controllers[J].J Virol,2012,86(7):3667-3674
[4]Han X,An M,Zhang M,et al.Identification of 3 distinct HIV-1 founding strains responsible forexpanding epidemic among men who have sex with men in 9 Chinese cities[J].J Acquir Immune Defic Syndr,2013,64(1):16-24.
[5]Wu J,Meng Z,Xu J,et al.New emerging recombinant HIV-1strains and close transmission linkage of HIV-1 strains in the Chinese MSM population indicate a new epidemic risk[J].PLo S One,2013,8(1):54322.
[6]Ondondo BO.The influence of delivery vectors on HIV vaccine efficacy[J].Front Microbiol,2014(5):439
[7]Graziani GM,Angel JB.Evaluating the efficacy of therapeutic HIV vaccines through analytical treatmentinterruptions[J].J Int AIDS Soc,2015,18(1):20497
[8]陈丹瑛,何小周,汪孟冉,等.含密码子优化的SIV gag基因的DNA疫苗与r Ad5疫苗构建及免疫原性评价[J].中华实验和临床病毒学杂志,2014,28(1):17-19.
[9]余炼.表达SIV及HIV基因的多载体疫苗联合应用的免疫策略及相关佐剂研究[D].北京工业大学,硕士论文,2016.
[10]阳凯,刘聪,陈慧萍,等.45例HIV抗体不确定者随访检测结果分析[J].公共卫生与预防医学,2016,27(04):107-109.