~(60)Co-γ射线辐射对荷兰鸢尾花色诱变效应的研究
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摘要
为探究不同~(60)Co-γ射线辐射剂量对荷兰鸢尾的诱变效应,以花深蓝色的荷兰鸢尾品种展翅种球为试验材料,分别进行0(CK)、3、5、7、10 Gy 5个剂量的辐射处理,并调查辐射后代的生长发育状况以及花色变异表现。结果表明,辐射处理对VM_1的生长具有抑制作用,但经过2代的栽培后VM_3生长指标恢复,变异特性稳定;受体的花色诱变效应和花色变化产生的方向与辐射剂量密切相关,7 Gy处理时,出现白色和紫色2种颜色;低剂量(3 Gy和5 Gy)处理下的花色更倾向于白色转变,而高剂量(10Gy)处理下更偏向于紫色方向变化。经多代选育,在VM_6中筛选到深蓝紫色、紫罗兰色和白色3个不同花色的变异新株系,表明~(60)Co-γ辐射诱变可以作为荷兰鸢尾新品种培育的有效途径。本研究结果为荷兰鸢尾种球~(60)Co-γ射线辐射诱变育种和选育观赏性状优良的新品种奠定了一定的理论基础。
To investigate the mutagenic effects of Iris hollandica in different radiation doses of ~(60)Co-γ rays,the seedballs of zhanchi with dark blue flower were used as test materials and were treated at 0( CK) 3,5,7 and 10 Gy doses,respectively,then the growth level and color variation of irradiated progeny were investigated. The results showed that the irradiation had an inhibitory effect on the growth of VM_1. However,after two generations of cultivation,the growth index of the VM_3 had recovered and variation characteristics were stable. The color mutation effects and color mutation direction were closely releted to the dose of radiation. The receptor appeared white and purple color variations at the 7 Gy treatment,and one-way transformation to white color at low dose of treatment( 3 Gy and SGy),while more biased towards purple variation through the semi-lethal dose of 10 Gy treatment. After several generations of breeding,three different lines of variations were selected at VM_6 generation,which demonstrats that ~(60)Co-γ ray radiation is a new effective method for new varieties breeding of Iris hollandica. The results of this study laid a theoretical foundation for the breeding of ~(60)Co-γ ray radiation mutation and the selection of new varieties with excellent ornamental traits.
To investigate the mutagenic effects of Iris hollandica in different radiation doses of ~(60)Co-γ rays,the seedballs of zhanchi with dark blue flower were used as test materials and were treated at 0( CK) 3,5,7 and 10 Gy doses,respectively,then the growth level and color variation of irradiated progeny were investigated. The results showed that the irradiation had an inhibitory effect on the growth of VM_1. However,after two generations of cultivation,the growth index of the VM_3 had recovered and variation characteristics were stable. The color mutation effects and color mutation direction were closely releted to the dose of radiation. The receptor appeared white and purple color variations at the 7 Gy treatment,and one-way transformation to white color at low dose of treatment( 3 Gy and SGy),while more biased towards purple variation through the semi-lethal dose of 10 Gy treatment. After several generations of breeding,three different lines of variations were selected at VM_6 generation,which demonstrats that ~(60)Co-γ ray radiation is a new effective method for new varieties breeding of Iris hollandica. The results of this study laid a theoretical foundation for the breeding of ~(60)Co-γ ray radiation mutation and the selection of new varieties with excellent ornamental traits.
引文
[1]北京林业大学园林系花卉教研组.花卉学[M].北京:中国林业出版社,1990
[2]夏宜平.切花周年生产技术[M].北京:中国农业出版社,2000
[3]Imanishi H.Cut flower production from bulbs in Japan[J].Journal of the Korean Society for Horticultural Science,1996,37(6):836-841
[4]Kim S J,Nam C W,Yoo D L,Ryu S Y,Kim K S.Effect of pretreatment solution on Iris hollandica flower:vase life,anthocyanin content,and peroxidase activity[J].Hort Science,2004,39(4):823-823
[5]林兵,黄敏玲,陈诗林,吴建设,叶贻源.荷兰鸢尾引种栽培试验[J].亚热带植物科学,2004,33(1):49-50
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[7]蔡曾煜.荷兰鸢尾[J].中国花卉盆景,2009(1):2-5
[8]黄敏玲,陈诗林,吴建设,林兵,叶贻源.低温和植物生长调节剂对荷兰鸢尾开花的效应[J].中南林学院学报,2003,23(5):34-37
[9]林兵,黄敏玲,陈源泉,吴建设,叶秀仙.种球冷藏处理及栽培方式对荷兰鸢尾花期调节的效应[J].亚热带植物科学,2005,34(2):51-52
[10]林兵,黄敏玲,陈源泉,吴建设,叶秀仙,钟淮钦.影响荷兰鸢尾开花的若干因素研究[C]∥中国园艺学会2007年学术年会论文集,北京:中国园艺学会,2007:333-337
[11]廖飞雄,潘瑞炽.60Co-γ辐射对菜心种子萌芽和幼苗生长的效应[J].核农学报,2001,15(1):6-10
[12]刘振国,史秀娟,任清盛,李立国,李庆芝,赵济红.生姜60Coγ辐射诱变育种研究[J].核农学报,2010,24(1):36-40
[13]张德建,梁发茂,胡刚,邢永忠,李志新.γ射线辐射‘9311’水稻突变体的筛选[J].植物遗传资源学报,2015,16(4):876-882
[14]张亚惠,周历萍,王淑珍,毛碧增.60Co-γ射线辐射草莓红颊诱变选育新品系的研究[J].核农学报,2018,32(8):1457-1465
[15]程金水.园林植物遗传育种学[M].北京:中国林业出版社,2000
[16]杨晓红,张克中.园林植物育种学[M].北京:气象出版社,2001
[17]王丹,任少雄,苏军,李卫锋,王熙.核技术在观赏植物诱变育种上的应用[J].核农学报,2004,18(6):443-447
[18]沈秋泉,杨建明,符锦康,徐忆中,陈世明,黎侠,杨文新.大麦品种浙原18的特征特性和栽培技术[J].浙江农业科学,1998,(5):229-230
[19]Jerzy M,Zalewska M.Polish cultivars of Dendranthema grandiflora Tzvelev and Gerbera jamesonii Bolus bred in vitro by induced mutations[J].Mutation Breeding Newsletter,1996,42:19
[20]史燕山,骆建霞,赵国防,秦桂萍,刘学恩.晚香玉60Co-γ射线辐射诱变适宜剂量的研究[J].园艺学报,2003,30(6):748-750
[21]林兵,陈诗林,黄敏玲,钟淮钦,陈源泉,吴建设,叶秀仙.60Coγ射线辐照对3种国兰生长的影响[J].核农学报,2009,23(2):244-247
[22]葛维亚,杨树华,陈林,赵滢,王甜甜,葛红.辐照对地被菊种子的生长效应[J].核农学报,2011,25(1):67-70
[23]蒋彧,何俊蓉,刘菲,王海娥,卓碧萍.60Coγ辐射兰花春剑隆昌素根状茎分化苗的ISSR分析[J].核农学报,2013,27(9):1247-1252
[24]王晶,刘录祥,赵世荣,郭会君,赵林姝,陈文华.60Co-γ射线对菊花组培苗的诱变效应[J].农业生物技术学报,2006,14(2):241-244
[25]刘丽强,刘军丽,张杰,耿慧,张玉萍,沈红香,高遐红,姚允聪.60Co-γ辐射对观赏海棠组培苗的诱变效应[J].中国农业科学,2010,43(20):4255-4264
[26]刘春贵,李风童,孙叶,袁媛,包建忠,陈秀兰.60Co-γ射线对路易斯安那鸢尾种子的辐射效应研究[J].核农学报,2018,32(1):1-7
[27]李风童,包建忠,孙叶,刘春贵,马辉,张甜,陈秀兰.60Co-γ射线辐照德国鸢尾杂交种子的生物效应[J].核农学报,2017,31(8):1469-1474
[28]林兵,钟淮钦,黄敏玲,吴建设,叶秀仙.60Co-γ射线对荷兰鸢尾的辐照效应[J].核农学报,2010,24(1):50-54
[29]包建忠,李风童,孙叶,刘春贵,马辉,张甜,陈秀兰.60Co-γ射线辐照大花君子兰种子对其萌发特性及其开花性状的影响[J].核农学报,2013,27(11):1681-1685
[30]朱校奇,周佳民,黄艳宁,鲁耀雄,李文革,罗志平,张天术.卷丹百合辐射诱变的生物学效应及变异研究初报[J].南方农业学报,2012,43(11):1638-1641
[31]包建忠,李风童,刘春贵,孙叶,马辉,张甜,陈秀兰.大花君子兰新品种扬君1号选育与栽培技术[J].核农学报,2014,28(11):1956-1962
[32]陆波,郑玉红,陈默,彭峰,束晓春,高风.60Co-γ射线对彩色马蹄莲Parfait的辐照效应及其在高温高湿胁迫下的生理响应[J].核农学报,2014,28(8):1353-1357
[2]夏宜平.切花周年生产技术[M].北京:中国农业出版社,2000
[3]Imanishi H.Cut flower production from bulbs in Japan[J].Journal of the Korean Society for Horticultural Science,1996,37(6):836-841
[4]Kim S J,Nam C W,Yoo D L,Ryu S Y,Kim K S.Effect of pretreatment solution on Iris hollandica flower:vase life,anthocyanin content,and peroxidase activity[J].Hort Science,2004,39(4):823-823
[5]林兵,黄敏玲,陈诗林,吴建设,叶贻源.荷兰鸢尾引种栽培试验[J].亚热带植物科学,2004,33(1):49-50
[6]王家强.荷兰鸢尾规模化生产[J].中国花卉园艺,2007(2):18-21
[7]蔡曾煜.荷兰鸢尾[J].中国花卉盆景,2009(1):2-5
[8]黄敏玲,陈诗林,吴建设,林兵,叶贻源.低温和植物生长调节剂对荷兰鸢尾开花的效应[J].中南林学院学报,2003,23(5):34-37
[9]林兵,黄敏玲,陈源泉,吴建设,叶秀仙.种球冷藏处理及栽培方式对荷兰鸢尾花期调节的效应[J].亚热带植物科学,2005,34(2):51-52
[10]林兵,黄敏玲,陈源泉,吴建设,叶秀仙,钟淮钦.影响荷兰鸢尾开花的若干因素研究[C]∥中国园艺学会2007年学术年会论文集,北京:中国园艺学会,2007:333-337
[11]廖飞雄,潘瑞炽.60Co-γ辐射对菜心种子萌芽和幼苗生长的效应[J].核农学报,2001,15(1):6-10
[12]刘振国,史秀娟,任清盛,李立国,李庆芝,赵济红.生姜60Coγ辐射诱变育种研究[J].核农学报,2010,24(1):36-40
[13]张德建,梁发茂,胡刚,邢永忠,李志新.γ射线辐射‘9311’水稻突变体的筛选[J].植物遗传资源学报,2015,16(4):876-882
[14]张亚惠,周历萍,王淑珍,毛碧增.60Co-γ射线辐射草莓红颊诱变选育新品系的研究[J].核农学报,2018,32(8):1457-1465
[15]程金水.园林植物遗传育种学[M].北京:中国林业出版社,2000
[16]杨晓红,张克中.园林植物育种学[M].北京:气象出版社,2001
[17]王丹,任少雄,苏军,李卫锋,王熙.核技术在观赏植物诱变育种上的应用[J].核农学报,2004,18(6):443-447
[18]沈秋泉,杨建明,符锦康,徐忆中,陈世明,黎侠,杨文新.大麦品种浙原18的特征特性和栽培技术[J].浙江农业科学,1998,(5):229-230
[19]Jerzy M,Zalewska M.Polish cultivars of Dendranthema grandiflora Tzvelev and Gerbera jamesonii Bolus bred in vitro by induced mutations[J].Mutation Breeding Newsletter,1996,42:19
[20]史燕山,骆建霞,赵国防,秦桂萍,刘学恩.晚香玉60Co-γ射线辐射诱变适宜剂量的研究[J].园艺学报,2003,30(6):748-750
[21]林兵,陈诗林,黄敏玲,钟淮钦,陈源泉,吴建设,叶秀仙.60Coγ射线辐照对3种国兰生长的影响[J].核农学报,2009,23(2):244-247
[22]葛维亚,杨树华,陈林,赵滢,王甜甜,葛红.辐照对地被菊种子的生长效应[J].核农学报,2011,25(1):67-70
[23]蒋彧,何俊蓉,刘菲,王海娥,卓碧萍.60Coγ辐射兰花春剑隆昌素根状茎分化苗的ISSR分析[J].核农学报,2013,27(9):1247-1252
[24]王晶,刘录祥,赵世荣,郭会君,赵林姝,陈文华.60Co-γ射线对菊花组培苗的诱变效应[J].农业生物技术学报,2006,14(2):241-244
[25]刘丽强,刘军丽,张杰,耿慧,张玉萍,沈红香,高遐红,姚允聪.60Co-γ辐射对观赏海棠组培苗的诱变效应[J].中国农业科学,2010,43(20):4255-4264
[26]刘春贵,李风童,孙叶,袁媛,包建忠,陈秀兰.60Co-γ射线对路易斯安那鸢尾种子的辐射效应研究[J].核农学报,2018,32(1):1-7
[27]李风童,包建忠,孙叶,刘春贵,马辉,张甜,陈秀兰.60Co-γ射线辐照德国鸢尾杂交种子的生物效应[J].核农学报,2017,31(8):1469-1474
[28]林兵,钟淮钦,黄敏玲,吴建设,叶秀仙.60Co-γ射线对荷兰鸢尾的辐照效应[J].核农学报,2010,24(1):50-54
[29]包建忠,李风童,孙叶,刘春贵,马辉,张甜,陈秀兰.60Co-γ射线辐照大花君子兰种子对其萌发特性及其开花性状的影响[J].核农学报,2013,27(11):1681-1685
[30]朱校奇,周佳民,黄艳宁,鲁耀雄,李文革,罗志平,张天术.卷丹百合辐射诱变的生物学效应及变异研究初报[J].南方农业学报,2012,43(11):1638-1641
[31]包建忠,李风童,刘春贵,孙叶,马辉,张甜,陈秀兰.大花君子兰新品种扬君1号选育与栽培技术[J].核农学报,2014,28(11):1956-1962
[32]陆波,郑玉红,陈默,彭峰,束晓春,高风.60Co-γ射线对彩色马蹄莲Parfait的辐照效应及其在高温高湿胁迫下的生理响应[J].核农学报,2014,28(8):1353-1357