双参通脉颗粒对心肌缺血损伤大鼠的保护作用实验研究
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摘要
目的:观察双参通脉颗粒对急性心肌缺血大鼠的保护作用。方法:将50只雄性SD大鼠随机分为5组,分别是对照组、模型组、药物低剂量组、药物中剂量组、药物高剂量组,每组10只。连续灌胃2周后,通过结扎大鼠左冠状动脉前降支建立急性心肌缺血模型;各组动物分别记录结扎前5min,结扎后5min、1h的心电图,应用生化分析仪测定大鼠血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)的含量。结果:与对照组比较,结扎后模型组及药物组ST段均抬高(P<0.05);与模型组比较,药物组CK、CKMB水平有不同程度的下降(P<0.05);不同组切片在光镜下有不同的病理变化。结论:双参通脉颗粒对急性心肌缺血大鼠具有保护作用。
Objective:To observe the protective effect of Shuangshen Tongmai Granule on acute myocardial ischemia in rats.Methods:50 male Sprague-Dawley rats were randomly divided into 5 groups,10 in each group:The control group,the model group,the drug low dose group,the drug middle dose group,and the drug high dose group.After two weeks of continuous gavage,an acute myocardial ischemia model was established by ligation of the left anterior descending coronary artery in rats;The animals in each group recorded the electrocardiogram 5 min before ligation and 5 min and 1 hafter ligation.Serum creatine kinase(CK)and creatine kinase isoenzyme(CK-MB)were measured by biochemical analyzer.Results:Compared with the control group,After the ligation,the ST segment of the model group and the drug group were elevated(P<0.05);Compared with the model group,the levels of CK and CKMB in the drug group decreased to different extents(P<0.05).Different groups of sections have different pathological changes under light microscopy.Conclusion:Shuangshen Tongmai Granule has protective effect on acute myocardial ischemia in rats.
Objective:To observe the protective effect of Shuangshen Tongmai Granule on acute myocardial ischemia in rats.Methods:50 male Sprague-Dawley rats were randomly divided into 5 groups,10 in each group:The control group,the model group,the drug low dose group,the drug middle dose group,and the drug high dose group.After two weeks of continuous gavage,an acute myocardial ischemia model was established by ligation of the left anterior descending coronary artery in rats;The animals in each group recorded the electrocardiogram 5 min before ligation and 5 min and 1 hafter ligation.Serum creatine kinase(CK)and creatine kinase isoenzyme(CK-MB)were measured by biochemical analyzer.Results:Compared with the control group,After the ligation,the ST segment of the model group and the drug group were elevated(P<0.05);Compared with the model group,the levels of CK and CKMB in the drug group decreased to different extents(P<0.05).Different groups of sections have different pathological changes under light microscopy.Conclusion:Shuangshen Tongmai Granule has protective effect on acute myocardial ischemia in rats.
引文
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[2]许波华,许立.中药抗心肌缺血作用机制的研究进展[J].中国实验方剂学杂志,2011,17(15):265-269.
[3]卢志强,张艳军,崔广智,等.心肌缺血模型的制作方法研究进展[J].中国药理学通报,2012,28(8):1053-1056.
[4]李峰杰,李贻奎.心肌梗死动物模型研究进展[J].中国药理学通报,2013,29(1):5-9.
[5]王勇,范书英.冠心病介入治疗的现状和展望[J].临床内科杂志,2015,32(1):5-9.
[6]张琦.金匮要略讲义[M].上海:上海科学技术出版社,2008:99-108.
[7]吴勉华,王新月.中医内科学[M].北京:中国中医药出版社,2012:134-143.
[8]陈蔚文.中药学[M].北京:人民卫生出版社,2012:270-402.
[9]戴光辉.冠心病心绞痛的中医药辨证论治研究进展[J].医药产业资讯,2006,3(20):153-154.
[10]陈新.黄宛临床心电图学[M].北京:人民卫生出版社,2008:43-87.
[11]葛均波,徐永健.内科学[M].北京:人民卫生出版社,2013:227-236.
[12]罗俭权,陶珊花,李竞春.新型心肌损伤标志物与传统心肌酶对诊断早期急性心肌梗死的敏感度和特异度评价[J].国际检验医学杂志,2017,38(3):417-418.
[13]姜文大,梁灵君,顾洁莹,等.人参皂苷Rg1对腹主动脉缩窄致大鼠心肌肥厚和TLR4的影响[J].中药药理与临床,2013,29(1):35-38.
[14]耿长山.黄芪的免疫药理作用研究[J].中西医结合杂志,2016,36(1):8-26.
[15]张翠英,章洪,戚琼华.川芎的有效成分及药理研究进展[J].辽宁中医杂志,2014,41(10):2264-2266.
[16]高兆慧,卢少海,赵启韬.中药保护心肌缺血的有效成分及作用机制[J].中华中医药杂志,2015,30(1):170-173.