鬼箭羽化学成分及体外抗氧化活性研究
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摘要
目的研究鬼箭羽中的化学成分。方法采用重结晶,层析柱色谱等方法分离纯化鬼箭羽75%的乙醇提取物,并鉴定化合物的结构,用质谱、核磁共振等方法 ;通过分光光度法测定化合物的DPPH的清除率。结果从鬼箭羽中共分离纯化得到了8个单体化合物,分别为:木栓酮(1)、豆甾醇(2)、24s-豆甾-4-烯-3-酮(3)、α-羟基棕榈酸甲酯(4)、3-(4-羟基-2-甲氧基苯基)-丙烯酸-4-羟基-3-甲氧基苯基酯(5)、山柰酚(6)、槲皮素(7)、原儿茶酸(8)。与Vc相比三种化合物清除DPPH的能力大小顺序为:槲皮素>原儿茶酸>Vc>山柰酚。结论化合物2、4、5为首次在鬼箭羽中分离得到,槲皮素、原儿茶酸的体外抗氧化活性强于Vc。
Objective To study the chemical composition of Euonymus alatu. Methods The chemical composition of 75% ethanol extract of Euonymus alatus are isolated and purified by various chromatographic and recrystallization methods. Their structures are elucidated on mass spectrometry and nuclear magnetic resonance methods. Their DPPH and IC_50 are evaluate by spectrophotometry. Results Eight compounds are isolated fscavenging efficiency from Euonymus alatus,and the structures are identified as friedelin(1), stigmasterol(2),24 s-stigmast-4-ene-3-dione(3), α-hydroxy methyl palmitate(4), 3-(4-hydroxy-2-methoxyphenyl)-propenoic acid4-hydroxy-3-methoxyphenyl ester(5), kaempferol(6), quercetin(7), protocatechuic acid(8). Compared with vc,the order of DPPH scavenging activity of the three chemical composition: quercetin > protocatechuic acid >Vc >kaempferol. Conclusion Compounds 2,4,5 are isolated from this plant for the first time. Quercetin and protocatechuic acid are stronger than Vc in vitro anti-oxidant activity.
Objective To study the chemical composition of Euonymus alatu. Methods The chemical composition of 75% ethanol extract of Euonymus alatus are isolated and purified by various chromatographic and recrystallization methods. Their structures are elucidated on mass spectrometry and nuclear magnetic resonance methods. Their DPPH and IC_50 are evaluate by spectrophotometry. Results Eight compounds are isolated fscavenging efficiency from Euonymus alatus,and the structures are identified as friedelin(1), stigmasterol(2),24 s-stigmast-4-ene-3-dione(3), α-hydroxy methyl palmitate(4), 3-(4-hydroxy-2-methoxyphenyl)-propenoic acid4-hydroxy-3-methoxyphenyl ester(5), kaempferol(6), quercetin(7), protocatechuic acid(8). Compared with vc,the order of DPPH scavenging activity of the three chemical composition: quercetin > protocatechuic acid >Vc >kaempferol. Conclusion Compounds 2,4,5 are isolated from this plant for the first time. Quercetin and protocatechuic acid are stronger than Vc in vitro anti-oxidant activity.
引文
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[14]郎素梅,朱丹妮,余伯阳,等.中药鬼箭羽降糖有效部位的药效学和化学研究[J].中国药科大学学报,2003,34(2):128.
[2]李玉杰,龚慕辛,来媛媛,等.鬼箭羽不同提取部位对糖尿病大鼠药理作用的研究[J].北京中医药大学学报,2010,33(31):179-182.
[3]江苏新医学院.中药大辞典[M].上海:上海科学技术出版社,1986:1695.
[4]汪德清,沈文梅,田亚平,等.黄芪的三种提取成分对氧自由基作用的影响[J].中国药理学报,1994,10(4):129-131.
[5]黄德斌,余昭芬.鬼箭羽三种提取物对氧自由基作用的影响[J].湖北民族学院学报医学版,2006,23(2):4-6.
[6]范金波,蔡茜彤,冯叙桥,等.5种天然多酚类化合物抗氧化活性的比较[J].食品与发酵工业,2014,(40)7:77-83.
[7]Martinaud A.,Mercier Y.,Marinova P.,et al.Comparison of oxidative processes on myofibrillar proteins from beef during maturation and by different model oxidation systems[J].Journal of Agricultural and Food Chemistry,1997,45:2481-2487.
[8]姜志义,周传澄,吕曙华,等.中药鬼箭羽化学成分的研究[J].南京药学院学报,1982(2):93-95.
[9]闫利华,徐丽珍,邹忠梅.小木通茎的化学成分研究[J].中草药,2007,38(3):340-342.
[10]陈彦超.蛤蟆油中甾体类化合物的研究[D].长春:长春中医药大学,2011.
[11]Ailgaierb H,Porallaa K,Jung G.ω-Cycloheptyl-α-hydroxyundecanoic Acid,a New Fatty Acid from a Thermo-acidophilic Bacillus Species[J].Liebigs Ann Chem,1985(2):378-382.
[12]Cheng F,Zou K,Zhu S.A New Phenylpropionate Derivative from the Rhizomes of Hedysarum polybotrys[J].Chinese Journal of Natural Medicines,2009,7(5):351-353.
[13]梁艳,于世锋,陈卫军,等.山柰酚的电喷雾质谱裂解途径[J].应用化学,2009,26(10):1250-1252.
[14]郎素梅,朱丹妮,余伯阳,等.中药鬼箭羽降糖有效部位的药效学和化学研究[J].中国药科大学学报,2003,34(2):128.
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