泛耐药鲍氏不动杆菌噬菌体的分离及生物学特性分析
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摘要
目的分离泛耐药鲍氏不动杆菌噬菌体并了解其生物学特性。方法噬菌体分离自不同来源的污水,采用了78株泛耐药鲍氏不动杆菌作为宿主菌,测定噬菌体的效价、裂解谱,并进行透射电子显微镜下观察等生物学特性分析。结果分离得到2株泛耐药鲍氏不动杆菌噬菌体AB46和AB54,均可形成透亮的噬菌斑,具有较强的裂解能力,电镜下显示为六边形结构,AB46具有尾部。AB46噬菌体和AB54噬菌体的效价分别是2.8×108pfu/ml和2.0×107pfu/ml。结论 2株鲍氏不动杆菌噬菌体均属于裂性噬菌体,其生物学特性并不完全相同。
Objective To isolate pandrug resistant Acinetobacter baumannii bacteriophages and study their biological character.Methods The bacteriophages were isolated from sewage of different sources with 78 different Acinetobacters as their hosts. The phage titer and fragmentation spectrum were determined, and their morphology was observed with electron microscope.Results Two different pandrug resistant Acinetobacter baumannii bacteriophages(AB46,AB54) were isolated. The new found Acinetobacter bacteriophages could produce transparent plaques and infect their hosts virulently. These phages had structures of hexagon when observed with electron microscope,and AB46 had tails. The phage titers of AB46 and AB54 were 2. 8 × 108 pfu/ml,2. 0 × 107 pfu/ml,respectively. Conclusion Two Acinetobacter baumannii bacteriophages were both virulent phage with different biological characters.
Objective To isolate pandrug resistant Acinetobacter baumannii bacteriophages and study their biological character.Methods The bacteriophages were isolated from sewage of different sources with 78 different Acinetobacters as their hosts. The phage titer and fragmentation spectrum were determined, and their morphology was observed with electron microscope.Results Two different pandrug resistant Acinetobacter baumannii bacteriophages(AB46,AB54) were isolated. The new found Acinetobacter bacteriophages could produce transparent plaques and infect their hosts virulently. These phages had structures of hexagon when observed with electron microscope,and AB46 had tails. The phage titers of AB46 and AB54 were 2. 8 × 108 pfu/ml,2. 0 × 107 pfu/ml,respectively. Conclusion Two Acinetobacter baumannii bacteriophages were both virulent phage with different biological characters.
引文
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[15]闫李侠,黄至澄,任应鹏,等.噬菌体治疗泛耐药鲍氏不动杆菌重症感染小鼠的研究[J].中国人兽共患病学报,2017,33(10):920-922.
[2]Dijkshoorn L,Nemec A,Selferr H.An increasing threat in hospitals:multidrug-resistant Aeinetobaeter baumannii[J].Nat Rev Microbiol,2007,5(12):939-951.
[3]Gong Y,Chen J,Liu C,et al.Comparison of pathogens anti antibiotic resistance of burn patients in the burn ICU or in the common burn ward[J].Burns,2014,40(3):402-407.
[4]胡付品,朱德妹,汪复,等.2014年CHINET中国细菌耐药性监测[J].中国感染与化疗杂志,2015,15(15):401-410.
[5]Karaiskos I,Giamarellou H.Muhidrug-resistant and extensively drug-resistant Gram-negative pathogens:current and emerging therapeutic approaches[J].Expert Opin Pharmacother,2014,15(10):1351-1370.
[6]Visea P,Seifert H,Towner KJ.Aeinetobaeter inketion-an emerging threat to human health[J].IUBMB Life,2011,63(12):1048-1054.
[7]Hauser AR,Meesas J,Moir DT.Beyond antibiotics new therapeutic approaches for bacterial infections[J].Clin Infect Dis,2016,63(1):89-95.
[8]Kutter EM,Kuhl SJ,Ahedon ST.Re-establishing a place for phage therapy in western medicine[J].Future Mierobiol,2015,10(5):685-688.
[9]龙智,周云芳.非抗生素物质抗菌治疗的研究现状[J].临床儿科杂志,2015,33(6):592-596.
[10]吴田顺,郭通,刘新.噬菌体治疗鲍曼不动杆菌感染的综述[J].沈阳医学院学报,2014,16(4):243-245.
[11]胡北,王晶,严群,等.噬菌体治疗实验性小鼠耐亚胺培南铜绿假单胞菌感染的研究[J].中华微生物学和免疫学杂志,2006,26(5):438-441.
[12]Aslanov BI,Iafaev RKh,Zueva LP.Mode of the rational use of Pseudomonas aeruginosa bacteriophages in therapeutic and epidemic control practice[J].Zh Mikrobiol Epidemiol Immunobiol,2003,5:72-76.
[13]Smiths HW,Huggine MB.Successful treatment of phage Escherichia coli infections in mice using phages:its general superiority over antibiotics[J].J Gen Microbiol,1982,128(2):307-318.
[14]Alexander S,Zemphira A,Glenn J.Bacteriophage therapy[J].Antimicrob Agents Chemother,2001,45(3):649.
[15]闫李侠,黄至澄,任应鹏,等.噬菌体治疗泛耐药鲍氏不动杆菌重症感染小鼠的研究[J].中国人兽共患病学报,2017,33(10):920-922.