三七总皂苷对高脂血症金黄地鼠降脂作用的研究
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摘要
目的:研究三七总皂苷对高脂血症金黄地鼠的降血脂作用。方法:采用金黄地鼠给予高脂饲料4周建立高血脂动物模型,随机分为正常组,高血脂模型组,血塞通组,12周后:检测血清中总胆固醇(TC),甘油三酯(TG),高密度脂蛋白胆固醇(HDL-C),低密度脂蛋白胆固醇(LDL-C)的含量;检测血清超氧化物歧化酶(SOD)活性及丙二醛(MDA)和游离脂肪酸(NEFA)的含量;HE染色观察肝脏的病理学变化。结果:与正常对照组相比,模型组金黄地鼠血清TC、TG、LDL-C含量显著升高,经过给药治疗后,与模型组相比,治疗组TC、TG、LDL-C、MDA、NEFA含量显著下降,SOD活性与模型组相比并未显著差异。肝脏组织HE染色显示,模型组肝细胞肿胀、坏死,有炎性细胞浸润,经给药治疗后有所好转。结论:三七总皂苷可降低高血脂地鼠的血脂水平,具有保肝功效,可能与其增强肌体抗脂质氧化能力有关。
Objective: To study the hypolipidemic effect of Panax notoginsenosidum on golden hamsters with hyperlipidemia. Methods: The golden hamsters were fed with high fat diet for 4 weeks to establish a hyperlipidemia animal models, and were randomly divided into normal group, hyperlipidemia model group, Xuesaitong group. After 12 weeks, the contents of total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C), and low density lipoprotein cholesterol(LDL-C) in serum were detected; the activity of superoxide dismutase(SOD), the contents of malondialdehyde(MDA) and free fatty acid(NEFA) in serum was detected. The hepatic pathological changes were observed by HE staining. Results: Compared with the normal control group, the content of TC, TG and LDL-C in the serum of the model group increased significantly. After treatment, compared with the model group, the content of TC, TG, LDL-C, MDA and NEFA in the treatment group decreased significantly, and the activity of SOD was not significantly different from that in the model group. HE staining showed that hepatocytes in the model group were swollen, necrotic and infiltrated with inflammatory cells, which were improved after treatment. Conclusion: Panax notoginsenosidum can reduce the level of blood lipid in hyperlipidemic hamsters, and has the effect of protecting liver, which may be related to enhancing the anti-lipid oxidation ability of the body.
Objective: To study the hypolipidemic effect of Panax notoginsenosidum on golden hamsters with hyperlipidemia. Methods: The golden hamsters were fed with high fat diet for 4 weeks to establish a hyperlipidemia animal models, and were randomly divided into normal group, hyperlipidemia model group, Xuesaitong group. After 12 weeks, the contents of total cholesterol(TC), triglyceride(TG), high density lipoprotein cholesterol(HDL-C), and low density lipoprotein cholesterol(LDL-C) in serum were detected; the activity of superoxide dismutase(SOD), the contents of malondialdehyde(MDA) and free fatty acid(NEFA) in serum was detected. The hepatic pathological changes were observed by HE staining. Results: Compared with the normal control group, the content of TC, TG and LDL-C in the serum of the model group increased significantly. After treatment, compared with the model group, the content of TC, TG, LDL-C, MDA and NEFA in the treatment group decreased significantly, and the activity of SOD was not significantly different from that in the model group. HE staining showed that hepatocytes in the model group were swollen, necrotic and infiltrated with inflammatory cells, which were improved after treatment. Conclusion: Panax notoginsenosidum can reduce the level of blood lipid in hyperlipidemic hamsters, and has the effect of protecting liver, which may be related to enhancing the anti-lipid oxidation ability of the body.
引文
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[2] STANCU C,SIMA A.Statins:mechanism of action and effects [J].J Cell Mol Med,2001,5(4):378-387
[3] 中华中医药学会.中医内科常见病诊疗指南西医疾病部分[S].北京:中国中医药出版社,2008.37
[4] TANIYASU S,TANAKA O,YANG C R,et al.Dammarane saponins of flower buds of Panax notoginseng(Sanchi-ginseng)[J].Planta Med,1982,44(2):124-125
[5] 徐叔云,卞如濂,陈修.药理实验方法学[M].3 版.北京:人民卫生出版,2002.1 085
[6] 胡慧明,朱彦陈,朱巧巧,等.实验性高脂血症动物模型比较分析[J].中国中药杂志,2016,41(20):3 709-3 714
[7] 徐敏,李平明.高脂血症动物模型的研究[J].吉林医学,2018,39(1):172-175
[8] 庄瑞丰.中药降血脂作用研究进展[J].河北医药,2009,31(9):1 119-1 121
[9] 董晓芳.降血脂中药的研究现状及进展[J].中医中药,2016,16(66):202
[10] SUN D Q,LI A W,LI J,et al.Changes of lipid peroxidation in carbon disulfide-treated rat nerve tissues and serum [J].Chem Biol Interact,2009,179(2-3):110-117
[11] CHEN E P,BITTNER H B,DAVIS R D,et al.Extracellular superoxide dismutase transgene overexpression preserves postischemic myocardial function in isolated murine hearts [J].Circulation,1996,94(9):412-417
[12] YOUNG J F,DRAGSTED L O,DANESHVAR B,et al.The effect of grape-skin extract on oxidative status [J].Br J Nutr,2000,84(4):505-513
[13] LIN Y L,CHENG C Y,LIN Y P,et al.Hypolipidemic effect of green tea leaves through induction of antioxidant and phase II enzymes including superoxide dismutase,catalase,and glutathione S-transferase in rats [J].J Agric Food Chem,1998,46(5):1 893-1 899