藤茶总黄酮提取物对糖尿病前期小鼠的干预作用
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摘要
本实验探究通过饮用藤茶的生活干预方式预防、延缓或治愈糖尿病前期的可行性。本实验以高脂饮食结合注射链脲佐菌素的方式构建了小鼠糖尿病前期模型,成模后将小鼠分为空白组、模型组、藤茶总黄酮提取物低剂量组(200 mg/kg mb)和高剂量组(500 mg/kg mb)、阿卡波糖组和阿卡波糖-藤茶总黄酮提取物联用组进行干预,并检测了小鼠的相关指标。研究结果表明:与模型组相比,高剂量干预组小鼠的空腹血糖、葡萄糖耐受性、糖化血清蛋白及胰岛素分泌水平均显著降低(P<0.05);干预组小鼠的炎症因子(白细胞介素4、肿瘤坏死因子、C反应蛋白)、血浆脂质指标(游离脂肪酸、甘油三酯、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇)、短链脂肪酸(乙酸、丙酸、丁酸)水平与模型组间均存在统计学差异(P<0.05)。一定剂量的藤茶黄酮提取物对糖尿病前期小鼠显示出有效干预作用,其可能通过抗炎、调节糖脂代谢及肠道菌群等方式来延缓或阻止小鼠糖尿病的进一步发展。
In order to explore the feasibility of preventing, delaying or curing prediabetes by drinking vine tea, a mouse model of prediabetes was established by feeding a high-sugar and high-fat diet and injection of streptozocin(STZ). Then the model mice were divided into model group, low-dose(200 mg/kg mb) and high-dose(500 mg/kg mb) groups orally administered with total flavonoid extract from vine tea, acarbose control group and acarbotane-flavonoid extract group. Parameters of interest were detected. The results showed that fasting blood glucose, glucose tolerance, glycosylated serum protein(GSP) and insulin secretion levels in the high-dose treatment group were significantly lower than those in the model group(P < 0.05). In addition, there was a statistical difference in inflammatory factors(interleukin 4, tumor necrosis factor-α and C reactive protein), plasma lipid indices(free fatty acids, triglyceride, low density lipoprotein cholesterol, total cholesterol and high density lipoprotein cholesterol) and cecal short chain fatty acid levels(acetic acid, propionic acid, and butyric acid) between the model and treatment groups(P < 0.05). A certain dose of total flavonoid extract from vine tea could delay or prevent the development of diabetes in mice by exerting anti-inflammatory activity and regulating glycolipid metabolism and the intestinal microflora.
In order to explore the feasibility of preventing, delaying or curing prediabetes by drinking vine tea, a mouse model of prediabetes was established by feeding a high-sugar and high-fat diet and injection of streptozocin(STZ). Then the model mice were divided into model group, low-dose(200 mg/kg mb) and high-dose(500 mg/kg mb) groups orally administered with total flavonoid extract from vine tea, acarbose control group and acarbotane-flavonoid extract group. Parameters of interest were detected. The results showed that fasting blood glucose, glucose tolerance, glycosylated serum protein(GSP) and insulin secretion levels in the high-dose treatment group were significantly lower than those in the model group(P < 0.05). In addition, there was a statistical difference in inflammatory factors(interleukin 4, tumor necrosis factor-α and C reactive protein), plasma lipid indices(free fatty acids, triglyceride, low density lipoprotein cholesterol, total cholesterol and high density lipoprotein cholesterol) and cecal short chain fatty acid levels(acetic acid, propionic acid, and butyric acid) between the model and treatment groups(P < 0.05). A certain dose of total flavonoid extract from vine tea could delay or prevent the development of diabetes in mice by exerting anti-inflammatory activity and regulating glycolipid metabolism and the intestinal microflora.
引文
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[17]徐正哲,王飞雪,王昊月,等.发酵桦褐孔菌对链脲佐菌素诱导的II型糖尿病大鼠降血糖作用的影响[J].食品与机械,2017,33(6):176-179.DOI:10.13652/j.issn.1003-5788.2017.06035.
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[19]WU B,JIE L,JIAN T L,et al.Dihydromyricetin protectsagainst diabetic cardiomyopathy in streptozotocin-induced diabetic mice[J].BioMed Research International,2017,2017(1):1-13.DOI:10.1155/2017/3764370.
[20]胡名媛,王锋,马永建,等.鱼油对KKAy糖尿病小鼠糖代谢及PI3K/Akt信号通路的影响[J].食品科学,2018,39(11):126-130.DOI:10.7506/spkx1002-6630-201811020.
[21]VOLEK J S,PHINNEY S D,FORSYITHE C E,et al.Carbohydrate restriction has a more favorable impact on the metabolic syndrome than a low fat diet[J].Lipids,2009,44(4):297-309.DOI:10.1007/s11745-008-3274-2.
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[23]CASSADER M,GAMBINO R,MUSSO G,et al.Postprandial triglyceride-rich lipoprotein metabolism and insulin sensitivity in nonalcoholic steatohepatitis patients[J].Lipids,2001,36(10):1117-1124.DOI:10.1007/s11745-001-0822-5.
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[25]KACZMARCZYK M M,MILLER M J,FREUND G G.The health benefits of dietary fiber:beyond the usual suspects of type 2 diabetes mellitus,cardiovascular disease and colon cancer[J].Metabolism,2012,61(8):1058-1066.DOI:10.1016/j.metabol.2012.01.017.
[26]YU J Y,SONG P G,PERRY R,et al.The Effectiveness of green tea or green tea extract on insulin resistance and glycemic control in type 2 diabetes mellitus:a meta-analysis[J].Diabetes and Metabolism Journal,2017,41(4):251-262.DOI:10.4093/dmj.2017.41.4.251.
[27]包艳春,关秀军,刘云涛.老年2型糖尿病患者血液脂肪细胞因子与代谢综合征的相关性[J].中国老年学杂志,2015(10):2667-2669.
[28]JUNG J H,KIM H S.The inhibitory effect of black soybean on hepatic cholesterol accumulation in high cholesterol and high fat diet-induced nonalcoholic fatty liver disease[J].Food and Chemical Toxicology,2013,60:404-412.DOI:10.1016/j.fct.2013.07.048.
[29]ZHOU A,HERGERT N,ROMPATO G,et al.Whole grain oats improve insulin sensitivity and plasma cholesterol profile and modify gut microbiota composition in C57BL/6J mice[J].Journal of Nutrition,2015,145(2):222-230.DOI:10.3945/jn.114.199778.
[30]SUEZ J,KOREM T,ZEEVI D,et al.Artificial sweeteners induce glucose intolerance by altering the gut microbiota[J].Nature,2014,514:181-186.DOI:10.1038/nature13793.
[31]LACH G,SCHELLEKENS H,DINAN T G,et al.Anxiety,depression,and the microbiome:a role for gut peptides[J].Neurotherapeutics,2018,15(1):36-59.DOI:10.1007/s13311-017-0585-0.
[32]顾宁宁,张兴德,郁红礼,等.基于16S rRNA基因测序的黄连对2型糖尿病大鼠肠道微生物多样性影响研究[J].中草药,2017,48(19):3998-4004.DOI:10.7501/j.issn.0253-2670.2017.19.015.
[33]LARSEN N,VOGENSEN F K,VAN DEN BERG F W,et al.Gut microbiota in human adults with type 2 diabetes differs from nondiabetic adults[J].PLoS ONE,2010,5(2):9085.DOI:10.1371/journal.pone.0009085.
[34]QIU J,REN C Z,FAN J F,et al.Antioxidant activities of aged oat vinegar in vitro and in mouse serum and liver[J].Journal of Science and Food Agriculture,2010,90:1951-1958.DOI:10.1002/jsfa.4040.
[35]KANAMOTO Y,YAMASHITA Y,NANBA F,et al.A black soybean seed coat extract prevents obesity and glucose intolerance by upregulating uncoupling proteins and down-regulating inflammatory cytokines in high-fat diet-fed mice[J].Journal of Agriculture and Food Chemistry,2011,59(16):8985-8993.DOI:10.1021/jf201471p.
[36]陈丽艳,夏延,陈鹏,等.玉米须水提物对糖尿病大鼠肠道菌群的调节作用[J].中国微生态学杂志,2017,29(12):1382-1386.DOI:10.13381/j.cnki.cjm.201712004.
[2]国际糖尿病联合会.IDF糖尿病地图集第8版[R].布鲁塞尔:国际糖尿病联合会,2017.
[3]世界卫生组织.全球糖尿病报告[R].日内瓦:世界卫生组织,2016.
[4]KANAT M,DE FRONZO R A,ABDULL-GHANI M A.Treatment of prediabetes[J].World Journal of Diabetes,2015,6(12):1207-1222.DOI:10.1142/9789814603324.0005.
[5]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2017年版)[R].北京:中华医学会糖尿病学分会,2017.
[6]潘慧敏.藤茶改善膜岛素抵抗的初步研究[D].北京:北京协和医学院,2015:5-10.
[7]卢威,秦晓改,王跃虎,等.二氢杨梅素对四氧嘧啶性糖尿病小鼠的降糖作用研究[J].中药药理与临床,2011(4):15-17.DOI:10.1007/s10570-010-9464-0.
[8]吴瑛,吴彬,万婧,等.藤茶提取物改善2型糖尿病大鼠胰岛素抵抗的作用[J].第三军医大学学报,2015,37(5):454-457.DOI:10.16016/j.1000-5404.20140908.
[9]周海云,王文清,施春阳,等.二氢杨梅素药理及药物相互作用研究进展[J].中草药,2018,49(14):3411-3417.DOI:10.7501/j.issn.0253-2670.2018.14.030.
[10]何桂霞,裴刚,李斌,等.二氢杨梅素的稳定性研究[J].中国新药杂志,2007,16(22):1888-1890.
[11]张学娟.茶的形态学、生物学特性及主要化学成分研究[D].武汉:华中农业大学,2008:10-23.
[12]孙文龙.天然多酚胰岛素增敏剂防治糖尿病的基础研究[D].大连:大连理工大学,2017:39-53.
[13]马梦梅.孜然膳食纤维改性及降血糖活性研究[D].北京:中国农业科学院,2016:67-73.
[14]国家中医药管理局编委会.中华本草[M].上海:上海科学技术出版社,2008:4229-5000.
[15]汪悦,杨阳,邬颖华.高能量饮食联合STZ诱导2型糖尿病肾病动物模型研究进展[J].世界最新医学信息文摘,2018,18(92):84-86.DOI:10.19613/j.cnki.1671-3141.2018.92.038.
[16]陈凯.2型糖尿病膳食营养干预防治策略的研究[D].重庆:第三军医大学,2016:8-28.
[17]徐正哲,王飞雪,王昊月,等.发酵桦褐孔菌对链脲佐菌素诱导的II型糖尿病大鼠降血糖作用的影响[J].食品与机械,2017,33(6):176-179.DOI:10.13652/j.issn.1003-5788.2017.06035.
[18]张思聪,夏文水,王斌,等.不同分子量壳聚糖对高脂膳食小鼠血糖的调节作用[J].食品与机械,2017,33(4):122-125.DOI:10.13652/j.issn.1003-5788.2017.04025.
[19]WU B,JIE L,JIAN T L,et al.Dihydromyricetin protectsagainst diabetic cardiomyopathy in streptozotocin-induced diabetic mice[J].BioMed Research International,2017,2017(1):1-13.DOI:10.1155/2017/3764370.
[20]胡名媛,王锋,马永建,等.鱼油对KKAy糖尿病小鼠糖代谢及PI3K/Akt信号通路的影响[J].食品科学,2018,39(11):126-130.DOI:10.7506/spkx1002-6630-201811020.
[21]VOLEK J S,PHINNEY S D,FORSYITHE C E,et al.Carbohydrate restriction has a more favorable impact on the metabolic syndrome than a low fat diet[J].Lipids,2009,44(4):297-309.DOI:10.1007/s11745-008-3274-2.
[22]MIRMIRAN P,ASGHARI G,FARCHADNEIAED H,et al.Low carbohydrate diet is associated with reduced risk of metabolic syndrome in Tehranian adults[J].International Journal of Food Sciences and Nutrition,2017,68(3):358-365.DOI:10.1080/09637486.2016.1242119.
[23]CASSADER M,GAMBINO R,MUSSO G,et al.Postprandial triglyceride-rich lipoprotein metabolism and insulin sensitivity in nonalcoholic steatohepatitis patients[J].Lipids,2001,36(10):1117-1124.DOI:10.1007/s11745-001-0822-5.
[24]COSTACOU T,MAYER-DAVIS E J.Nutrition and prevention of type 2 diabetes[J].Annual Review of Nutrition,2003,23(1):147-170.DOI:10.1146/annurev.nutr.23.011702.073027.
[25]KACZMARCZYK M M,MILLER M J,FREUND G G.The health benefits of dietary fiber:beyond the usual suspects of type 2 diabetes mellitus,cardiovascular disease and colon cancer[J].Metabolism,2012,61(8):1058-1066.DOI:10.1016/j.metabol.2012.01.017.
[26]YU J Y,SONG P G,PERRY R,et al.The Effectiveness of green tea or green tea extract on insulin resistance and glycemic control in type 2 diabetes mellitus:a meta-analysis[J].Diabetes and Metabolism Journal,2017,41(4):251-262.DOI:10.4093/dmj.2017.41.4.251.
[27]包艳春,关秀军,刘云涛.老年2型糖尿病患者血液脂肪细胞因子与代谢综合征的相关性[J].中国老年学杂志,2015(10):2667-2669.
[28]JUNG J H,KIM H S.The inhibitory effect of black soybean on hepatic cholesterol accumulation in high cholesterol and high fat diet-induced nonalcoholic fatty liver disease[J].Food and Chemical Toxicology,2013,60:404-412.DOI:10.1016/j.fct.2013.07.048.
[29]ZHOU A,HERGERT N,ROMPATO G,et al.Whole grain oats improve insulin sensitivity and plasma cholesterol profile and modify gut microbiota composition in C57BL/6J mice[J].Journal of Nutrition,2015,145(2):222-230.DOI:10.3945/jn.114.199778.
[30]SUEZ J,KOREM T,ZEEVI D,et al.Artificial sweeteners induce glucose intolerance by altering the gut microbiota[J].Nature,2014,514:181-186.DOI:10.1038/nature13793.
[31]LACH G,SCHELLEKENS H,DINAN T G,et al.Anxiety,depression,and the microbiome:a role for gut peptides[J].Neurotherapeutics,2018,15(1):36-59.DOI:10.1007/s13311-017-0585-0.
[32]顾宁宁,张兴德,郁红礼,等.基于16S rRNA基因测序的黄连对2型糖尿病大鼠肠道微生物多样性影响研究[J].中草药,2017,48(19):3998-4004.DOI:10.7501/j.issn.0253-2670.2017.19.015.
[33]LARSEN N,VOGENSEN F K,VAN DEN BERG F W,et al.Gut microbiota in human adults with type 2 diabetes differs from nondiabetic adults[J].PLoS ONE,2010,5(2):9085.DOI:10.1371/journal.pone.0009085.
[34]QIU J,REN C Z,FAN J F,et al.Antioxidant activities of aged oat vinegar in vitro and in mouse serum and liver[J].Journal of Science and Food Agriculture,2010,90:1951-1958.DOI:10.1002/jsfa.4040.
[35]KANAMOTO Y,YAMASHITA Y,NANBA F,et al.A black soybean seed coat extract prevents obesity and glucose intolerance by upregulating uncoupling proteins and down-regulating inflammatory cytokines in high-fat diet-fed mice[J].Journal of Agriculture and Food Chemistry,2011,59(16):8985-8993.DOI:10.1021/jf201471p.
[36]陈丽艳,夏延,陈鹏,等.玉米须水提物对糖尿病大鼠肠道菌群的调节作用[J].中国微生态学杂志,2017,29(12):1382-1386.DOI:10.13381/j.cnki.cjm.201712004.
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