不同遮荫处理对蒲公英生长及总黄酮含量的影响
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摘要
【目的】为建立优质、高产的人工设施蒲公英栽培体系。【方法】采用温室分区栽培蒲公英并进行人工遮荫的试验方法,设置5个透光率梯度处理(100%、82.6%、60.1%、37.8%、14.5%),测定蒲公英的株高、茎粗、叶片数、叶片长宽、地上干鲜重及叶片中总黄酮含量指标。【结果】透光率高于60.1%时,降低光强会明显抑制蒲公英的茎粗、叶片数、叶片宽及地上干鲜质量;透光率100%的自然光强下蒲公英总黄酮含量最高为60.60mg/g,总黄酮含量生长后期>中期>前期。【结论】透光率100%自然光强下蒲公英的株高、茎粗、叶片数、叶片长宽、地上干鲜质量及总黄酮含量均为最高。重度遮荫不利于蒲公英叶片总黄酮的积累,同一光强处理下,总黄酮含量生长后期明显大于生长前期。
【Objective】The objective of this research is to establish a high quality,high yield cultivation system of taraxacum.【Methods】Five light transmittance gradients(100%,82.6%,60.1%,37.8%,14.5%)were set up in the greenhouse to cultivate taraxacum.The plant height,stem diameter,leaf number,leaf length/width,aboveground dry/fresh weight and total flavone content were measured in leaves.【Result】The total flavone content was 60.60 mg/g which is the highest when the light transmittance was 100%,The total flavone content was not significant when the light transmittance was lower than 60.1%.The total flavone content in later growth period is higher than that in the other two stages.Reducing the light intensity will significantly inhibit the plant height of taraxacum,and when the light transmittance is higher than 60.1%,reducing the light intensity will significantly inhibit the stem diameter,leaf number,leaf length/width and dry/fresh weight.When the light transmittance is lower than 60.1%,the light intensity is less significant on the stem diameter,leaf number,leaf length/width and dry/fresh weigh,but it will significantly inhibit the growth of the leaves.【Conclusion】Heavy shading is not conducive to the accumulation of total flavone.Under the same light intensity,the total flavone content in later stage was significantly higher than that in the early growth stage.
【Objective】The objective of this research is to establish a high quality,high yield cultivation system of taraxacum.【Methods】Five light transmittance gradients(100%,82.6%,60.1%,37.8%,14.5%)were set up in the greenhouse to cultivate taraxacum.The plant height,stem diameter,leaf number,leaf length/width,aboveground dry/fresh weight and total flavone content were measured in leaves.【Result】The total flavone content was 60.60 mg/g which is the highest when the light transmittance was 100%,The total flavone content was not significant when the light transmittance was lower than 60.1%.The total flavone content in later growth period is higher than that in the other two stages.Reducing the light intensity will significantly inhibit the plant height of taraxacum,and when the light transmittance is higher than 60.1%,reducing the light intensity will significantly inhibit the stem diameter,leaf number,leaf length/width and dry/fresh weight.When the light transmittance is lower than 60.1%,the light intensity is less significant on the stem diameter,leaf number,leaf length/width and dry/fresh weigh,but it will significantly inhibit the growth of the leaves.【Conclusion】Heavy shading is not conducive to the accumulation of total flavone.Under the same light intensity,the total flavone content in later stage was significantly higher than that in the early growth stage.
引文
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[15]朱肖锋,周守标,杨集辉,等.不同光照强度对马蹄金叶的特征及总黄酮含量的影响[J].激光生物学报,2009,18(1):62-66
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[17]杨岚,李华峰,刁海鹏,等.蒲公英花中总酚酸和总黄酮含量测定及其抗氧化性能研究[J].食品科学,2011,32(17):160-163
[18]Zoratti L,Karppinen K,Luengo Escobar A,et al.Lightcontrolled flavonoid biosynthesis in fruits[J].Frontiers in Plant Science,2014,5:534
[19]潘俊倩,佟曦然,郭宝林.光对植物黄酮类化合物的影响研究进展[J].中国中药杂志,2016,41(21):3897-3903
[2]Ovadje P,Hamm C,Pandey S.Efficient induction of extrinsic cell death by dandelion root extract in human chronic myelomonocytic leukemia(CMML)cells[J].PloS One,2012,7(2):e30604
[3]Chatterjee S J,Ovadje P,Mousa M,et al.The efficacy of dandelion root extract in inducing apoptosis in drug-resistant human melanoma cells[J].Evidence-Based Complementary and Alternative Medicine,2011(1741-427X):129045
[4]Ovadje P,Chochkeh M,Akbari-Asl P,et al.Selective induction of apoptosis and autophagy through treatment with dandelion root extract in human pancreatic cancer cells[J].Pancreas,2012,41(7):1039-1047
[5]吴杰,黄丹.蒲公英黄酮生物活性研究[J].饲料研究,2016(4):34-50
[6]呼永华.蒲公英的抗癌机理研究[J].西部中医药,2018,31(1):132-134
[7]谢沈阳,杨晓源,丁章贵,等.蒲公英的化学成份及其药理作用[J].天然产物研究与开发,2012,24(S1):141-151
[8]丁惠,张馨方,纪文华,等.蒲公英药用研究进展[J].辽宁中医药大学学报,2018,20(9):156-159
[9]陈景耀,吴国荣,王习达,等.蒲公英黄酮类物质的抗氧化活性[J].南京师大学报(自然科学版),2005(1):84-87
[10]杨文志,杜跃中,高宇,等.蒲公英总黄酮的研究进展[J].人参研究,2017,29(1):52-55
[11]芦晓磊,宁伟,汤贺,等.光照强度对马齿苋生长及光合特性的影响[J].华北农学报,2008(2):41-44
[12]张丽娟,曲继松,李堃,等.驯化栽培中遮荫处理对长白山茖葱生长发育的影响[J].北方园艺,2018(16):76-80
[13]Ferreres F,Figueiredo R,Bettencourt S,et al.Identification of phenolic compounds in isolated vacuoles of the medicinal[J].Journal of Experimental Botany,2011,62(8):2841-2854
[14]赵英明,范文丽.光照强度对蒲公英生长的影响[J].辽宁农业科学,2010(3):89-91
[15]朱肖锋,周守标,杨集辉,等.不同光照强度对马蹄金叶的特征及总黄酮含量的影响[J].激光生物学报,2009,18(1):62-66
[16]朱灿灿.银杏叶次生代谢产物的环境诱导机制及其调控[D].南京:南京林业大学,2010
[17]杨岚,李华峰,刁海鹏,等.蒲公英花中总酚酸和总黄酮含量测定及其抗氧化性能研究[J].食品科学,2011,32(17):160-163
[18]Zoratti L,Karppinen K,Luengo Escobar A,et al.Lightcontrolled flavonoid biosynthesis in fruits[J].Frontiers in Plant Science,2014,5:534
[19]潘俊倩,佟曦然,郭宝林.光对植物黄酮类化合物的影响研究进展[J].中国中药杂志,2016,41(21):3897-3903