1株红树林可口革囊星虫共附生放线菌抑制COX-2活性成分的研究
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摘要
目的从红树林可口革囊星虫表皮中分离共附生放线菌并进行抑制COX-2活性筛选,对其中1株具有较强活性菌株PE05的活性成分进行研究。方法采用稀释涂布法对红树林可口革囊星虫共附生放线菌进行分离,通过COX抑制活性试剂盒筛选菌株发酵产物抑制COX-2的活性。结合生物活性和薄层色谱检测,以PE05作为目标菌株,使用多种柱层析、重结晶等方法对发酵产物粗提物进行分离纯化,并通过现代波谱学技术鉴定化学结构。结果从红树林可口革囊星虫表皮中分离得到11株共附生放线菌,对其发酵产物进行活性筛选显示其中6株具有较好的抑制COX-2活性。从高活性菌株PE05中分离得到5个化合物,分别鉴定为:香草酸(1)、对羟基苯甲酸(2)、苯乙酸(3)、烟酸(4)、环(脯-亮)二肽(5)。结论从红树林可口革囊星虫共附生放线菌中发现PE05能产生良好COX-2抑制活性的代谢产物,该结果为从红树林生态系统中发掘新型的COX-2抑制剂提供了一种新的策略。
Objective To isolate and screen Phascolosoma esculenta symbiotic actinomycetes from mangrove for COX-2 inhibitory activity,and study secondary metabolites from the active strain PE05. Methods The symbiotic actinomycetes were isolated using dilution-spreading-plate method. The COX-2 inhibitory activities of fermentation products from all of strains were evaluated by COX inhibitor screening assay kit. PE05 was confirmed as the target strain and isolated by various column chromatography and recrystallization. Moreover,the structures of pure compounds were identified by spectroscopic methods. Results Eleven symbiotic actinomycetes were isolated from Phascolosoma esculenta,and six of them showed COX-2 inhibitory activities. Five compounds were obtained from PE05,which were identified as vanillic acid(1),4-hydroxybenzoie acid(2),phenylethyl acid(3),nicotinic acid(4),cyclo-(Pro-Leu)(5). Conclusion PE05 of Phascolosoma esculenta could be a suitable candidate as a potential COX-2 inhibitor producer,which will provide a new strategy for COX-2 inhibitor discovery.
Objective To isolate and screen Phascolosoma esculenta symbiotic actinomycetes from mangrove for COX-2 inhibitory activity,and study secondary metabolites from the active strain PE05. Methods The symbiotic actinomycetes were isolated using dilution-spreading-plate method. The COX-2 inhibitory activities of fermentation products from all of strains were evaluated by COX inhibitor screening assay kit. PE05 was confirmed as the target strain and isolated by various column chromatography and recrystallization. Moreover,the structures of pure compounds were identified by spectroscopic methods. Results Eleven symbiotic actinomycetes were isolated from Phascolosoma esculenta,and six of them showed COX-2 inhibitory activities. Five compounds were obtained from PE05,which were identified as vanillic acid(1),4-hydroxybenzoie acid(2),phenylethyl acid(3),nicotinic acid(4),cyclo-(Pro-Leu)(5). Conclusion PE05 of Phascolosoma esculenta could be a suitable candidate as a potential COX-2 inhibitor producer,which will provide a new strategy for COX-2 inhibitor discovery.
引文
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[2] 姜晓虹.选择性COX-2抑制剂塞来昔布的研究及应用进展[J]. 杭州师范学院学报(医学版),2008,28(6):432-436.
[3] 尹耕,谢其冰.环氧化酶-2抑制剂研究进展[J].现代预防医学,2009,36(3):599-601.
[4] 周婷婷.两株海洋放线菌次级代谢产物的研究[D]. 广州:广东药科大学,2017.
[5] EL-NEKETI M,EBRAHIM W,LIN W,et al. Alkaloids and polyketides from Penicillium citrinum,an endophyte isolated from the Moroccan plant Ceratonia siliqua[J]. J Nat Prod,2013,76(6):1099-1104.
[6] 赵婷婷,常一民,王俊潇,等.海洋放线菌次级代谢产物及其抗菌、抗病毒、抗肿瘤活性研究进展[J].中国海洋药物,2018,37(4):57-72.
[7] 徐志勇,冯昭,徐静.红树林微生物抗菌活性成分研究进展[J].中国抗生素杂志,2017,42(4):241-254.
[8] 梁倩蓉,于曙光,刘顺,等.可口革囊星虫Phasolosma esculenta体腔液的抗菌活性研究[J].海洋学研究,2015,33(2):70-75.
[9] 布坎南 R E,吉本斯 N E. 伯杰细菌鉴定手册[M].8版.北京:科学出版社,1984:1038-1039.
[10] 中国科学院微生物研究所放线菌分类组.链霉菌鉴定手册[M].北京:科学出版社,1975:56-57.
[11] 孙浩理,丁刚,宋波,等.石柑子脂溶性化学成分研究[J].中国药学杂志,2015,50(14):1186-1189.
[12] 陈铭祥,杨雪梅,关睿,等.独子藤根皮化学成分研究[J].中国药学杂志,2018,53(15):1265-1272.
[13] 郑茜,张庆贺,卢丹,等.吉林产玛咖的化学成分研究[J].中草药,2014,45(17):2457-2460.
[14] 郑宏亮,图力古尔,包海鹰,等.荷叶离褶伞的化学成分研究[J].中国中药杂志,2013,38(24):4335-4339.
[15] 曹家庆.滇桂艾纳香的化学成分研究[D]. 沈阳:沈阳药科大学,2007.
[16] 林珠灿.景天三七止血、抗炎物质基础与质量评价的研究[D].南京:南京中医药大学,2014.