菜用黄麻种质萌发期耐盐性评价
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摘要
为明确不同盐胁迫对菜用黄麻种子萌发的影响,并综合评价菜用黄麻种质的耐盐性,本研究以13份菜用黄麻种质为试验材料,以2份纤用黄麻为参照种质,分别测定在不同浓度(0、50、100、150、200 mmol/L)的两种单盐NaCl、Na2SO4处理下种子的发芽率、发芽势、胚芽长度、胚根长度,采用隶属函数法和系统聚类分析法对其耐盐性进行综合评价。结果表明,不同种类和不同浓度的盐胁迫对菜用黄麻发芽势、发芽率、胚根长度、胚芽长度存在不同程度的影响。随着盐浓度的增加菜用黄麻各指标均在盐浓度为200 mmol/L显著低于对照。NaCl胁迫对菜用黄麻萌发期各指标的抑制作用由大到小依次为胚根长>发芽势>胚芽长>发芽率,Na2SO4胁迫对菜用黄麻萌发期各指标的抑制作用由大到小依次为胚根长>胚芽长>发芽势>发芽率。Na2SO4对菜用黄麻种子萌发的抑制作用高于Na Cl。根据对13份菜用黄麻种质以及2份参照种质的综合评价及聚类分析,将15份黄麻种质划分为高耐盐、中度耐盐、盐敏感3种类型,筛选出高耐Na Cl的菜用黄麻4份分别为14MCB-1、粤引2号、埃及麻菜、福农1号,高耐Na2SO4种质1份为桂麻菜2号及对2种单盐均表现敏感的菜用黄麻种质平果麻菜。
The objective of this study aimed to evaluate salt tolerance of vegetable jute,via under different salt stress conditions on and comprehensively evaluate the salt tolerance.In this study,13 vegetable jute germplasms and 2 jute which used as control were treatmented with NaCl and Na2 SO4 at different concentrations of 0,50,100,150,200 mmol/L,respectively.Then germination potential,germination rate,sprout length and radical length were measured after salt treatment.The 13 vegetable jute germplasms were evaluated and classified using subordination function method and hierarchical cluster analysis.The result showed the different kinds and concentrations salt stress have different effects on germination potential,germination rate,sprout length and radical length of vegetable jute.With the increase of salt concentration,all indexes were significantly lower than the control 200 mmol/L.According to the degree of inhibit of NaCl stress,the indexes of jute germination were from large to small radical length,germination potential,sprout length,germination rate.According to the degree of inhibit of Na2 SO4 stress,the indexes of jute germination were from large to small radical length,sprout length,germination potential,germination rate.Na2 SO4 stress on the germination of vegetable jute was higher than NaCl.Based on the results,13 vegetable jute germplasms and 2 control lines could be classified into groups,including high salt tolerance,moderate salt tolerance,salt sensitivity.Finally,14 MCB-1,Yueyin No.2,Egypt macai,Funong No.1 were highly NaCl tolerant,Guimacai No.2 was highly Na2 SO4 tolerant,the Pingguomacai showed salt sensitivity to both salt.
The objective of this study aimed to evaluate salt tolerance of vegetable jute,via under different salt stress conditions on and comprehensively evaluate the salt tolerance.In this study,13 vegetable jute germplasms and 2 jute which used as control were treatmented with NaCl and Na2 SO4 at different concentrations of 0,50,100,150,200 mmol/L,respectively.Then germination potential,germination rate,sprout length and radical length were measured after salt treatment.The 13 vegetable jute germplasms were evaluated and classified using subordination function method and hierarchical cluster analysis.The result showed the different kinds and concentrations salt stress have different effects on germination potential,germination rate,sprout length and radical length of vegetable jute.With the increase of salt concentration,all indexes were significantly lower than the control 200 mmol/L.According to the degree of inhibit of NaCl stress,the indexes of jute germination were from large to small radical length,germination potential,sprout length,germination rate.According to the degree of inhibit of Na2 SO4 stress,the indexes of jute germination were from large to small radical length,sprout length,germination potential,germination rate.Na2 SO4 stress on the germination of vegetable jute was higher than NaCl.Based on the results,13 vegetable jute germplasms and 2 control lines could be classified into groups,including high salt tolerance,moderate salt tolerance,salt sensitivity.Finally,14 MCB-1,Yueyin No.2,Egypt macai,Funong No.1 were highly NaCl tolerant,Guimacai No.2 was highly Na2 SO4 tolerant,the Pingguomacai showed salt sensitivity to both salt.
引文
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