PPAR-γ基因多态性与子痫前期易感性的研究
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摘要
目的探讨子痫前期(PE)患者过氧化物酶体增殖物激活受体-γ(PPAR-γ)基因多态性与子痫前期的相关性。方法选取2016年5月至2018年10月在本院住院分娩的PE患者110例和同期正常妊娠孕产妇110例为研究对象,采用SNa Pshot技术检测PPAR-γ基因的rs10865710和rs4684847两个位点分型。结果⑴rs10865710位点:对照组CC、CG、GG基因型频率分别是44. 55%、42. 73%、12. 73%,PE组CC、CG、GG基因型频率分别是31. 82%、44. 55%、23. 64%,两组基因型频数分布比较差异有临界意义(χ~2=5. 975,P=0. 050);对照组C、G等位基因频率分别是65. 91%、34. 09%,PE组C、G等位基因频率分别是54. 09%、45. 91%,两组等位基因频数分布比较差异有统计学意义(χ~2=6. 402,P=0. 015)。⑵rs4684847位点:对照组CC、CT、TT基因型频率分别是94. 55%、5. 45%、0,PE组CC、CT、TT基因型频率分别是84. 55%、15. 45%、0,两组基因型频数分布比较差异有统计学意义(χ~2=5. 875,P=0. 015);对照组C、T等位基因频率分别是97. 27%、2. 73%,PE组C、T等位基因频率分别是92. 27%、7. 73%,两组等位基因频数分布比较差异有统计学意义(χ~2=5. 551,P=0. 030)。⑶rs10865710位点GG基因型和G等位基因在PE组的频率均明显高于对照组[OR(95%CI)=2. 600 (1. 190-5. 679),P=0. 021;OR (95%CI)=1. 64 (1. 117-2. 411),P=0. 015]。rs4684847位点CT基因型和T等位基因在PE组的频率均明显高于对照组[OR(95%CI)=3. 168(1. 199-8. 374),P=0. 026; OR (95%CI)=2. 987 (1. 155-7. 726),P=0. 030]。结论PPAR-γ基因rs10865710和rs4684847两个位点单核苷酸多态性可能与中国人群子痫前期的易感性有关。
Objective To investigate the correlation between peroxisome proliferator-activated receptor-gamma(PPAR-γ) gene polymorphism and preeclampsia(PE). Methods 110 PE patients and 110 normal pregnants who delivered in our hospital from May 2016 to October 2018 were selected as the study subjects. Detection of two loci of PPAR-γ gene by SNaP shot technique: rs10865710 and rs4684847. Results ⑴ rs10865710: The genotype frequencies of CC,CG and GG in the control group were 44. 55%,42. 73% and 12. 73%,respectively,and those in the PE group were 31. 82%,44. 55% and 23. 64%,respectively. There was a critical difference in the distribution of genotype frequencies between the two groups(χ~2= 5. 975,P = 0. 050); The frequencies of C and G alleles were 65. 91% and 34. 09% in the control group,54. 09% and 45. 91% in the PE group,respectively. There was significant difference in the frequency distribution of C and G alleles between the two groups(χ~2= 6. 402,P = 0. 015). ⑵ rs4684847:the genotype frequencies of CC,CT and TT in control group were 94. 55%,5. 45%,0,and those in PE group were 84. 55%,15. 45% and 0,respectively,with significant difference in the distribution of genotype frequencies between the two groups(χ~2= 5. 875,P = 0. 015). The frequencies of C and T alleles in control group were 97. 27%,2. 73% and those in PE group were 92. 27% and 7. 73%,respectively,with significant difference in allele frequency distribution between the two groups(χ~2= 5. 551,P = 0. 030). ⑶ The frequency of GG genotype and G allele at rs10865710 locus in PE group was significantly higher than that in control group [OR(95% CI) = 2. 600(1. 190-5. 679),P = 0. 021; OR(95% CI) = 1. 64(1. 117-2. 411),P = 0. 015]. The frequencies of CT genotype and T allele at rs4684847 locus in PE group were significantly higher than those in control group [OR(95% CI) = 3. 168(1. 199-8. 374),P = 0. 026; OR(95% CI) = 2. 987(1. 155-7. 726),P = 0. 030]. Conclusions The single nucleotide polymorphisms of PPAR-γ gene rs10865710 and rs4684847 may be related to the susceptibility to preeclampsia in Chinese population.
Objective To investigate the correlation between peroxisome proliferator-activated receptor-gamma(PPAR-γ) gene polymorphism and preeclampsia(PE). Methods 110 PE patients and 110 normal pregnants who delivered in our hospital from May 2016 to October 2018 were selected as the study subjects. Detection of two loci of PPAR-γ gene by SNaP shot technique: rs10865710 and rs4684847. Results ⑴ rs10865710: The genotype frequencies of CC,CG and GG in the control group were 44. 55%,42. 73% and 12. 73%,respectively,and those in the PE group were 31. 82%,44. 55% and 23. 64%,respectively. There was a critical difference in the distribution of genotype frequencies between the two groups(χ~2= 5. 975,P = 0. 050); The frequencies of C and G alleles were 65. 91% and 34. 09% in the control group,54. 09% and 45. 91% in the PE group,respectively. There was significant difference in the frequency distribution of C and G alleles between the two groups(χ~2= 6. 402,P = 0. 015). ⑵ rs4684847:the genotype frequencies of CC,CT and TT in control group were 94. 55%,5. 45%,0,and those in PE group were 84. 55%,15. 45% and 0,respectively,with significant difference in the distribution of genotype frequencies between the two groups(χ~2= 5. 875,P = 0. 015). The frequencies of C and T alleles in control group were 97. 27%,2. 73% and those in PE group were 92. 27% and 7. 73%,respectively,with significant difference in allele frequency distribution between the two groups(χ~2= 5. 551,P = 0. 030). ⑶ The frequency of GG genotype and G allele at rs10865710 locus in PE group was significantly higher than that in control group [OR(95% CI) = 2. 600(1. 190-5. 679),P = 0. 021; OR(95% CI) = 1. 64(1. 117-2. 411),P = 0. 015]. The frequencies of CT genotype and T allele at rs4684847 locus in PE group were significantly higher than those in control group [OR(95% CI) = 3. 168(1. 199-8. 374),P = 0. 026; OR(95% CI) = 2. 987(1. 155-7. 726),P = 0. 030]. Conclusions The single nucleotide polymorphisms of PPAR-γ gene rs10865710 and rs4684847 may be related to the susceptibility to preeclampsia in Chinese population.
引文
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[10]丁一,郭志荣,武鸣,等.PPARD-87T>C与PPARA和PPARG单核苷酸多态性之间的交互作用对腹型肥胖的影响[J].中华医学杂志,2012,92(22):1517-1521.DOI:10.3760/cma.j.issn.0376-2491.2012.22.004.
[2]Yang J,Gong H,Liu W,et al.The association of Pro12Ala polymorphism in the peroxisome proliferator-activated receptor-gamma2gene with the metabolic characteristics in Chinese women with polycystic ovary syndrome[J].Int J Clin Exp Pathol,2013,6(9):1894-1902.
[3]Paramasivam D,Safi SZ,Qvist R,et al.Role of PPARG(Pro12Ala)in Malaysian type 2 diabetes mellitus patients[J].Int J Diabetes Dev Ctries,2016,36(4):449-456.DOI:10.1007/s13410-015-0462-5.
[4]Lu Z,Dong B,Mo X,et al.Pro12Ala polymorphism in PPARgamma 2 associated with essential hypertension in Chinese nonagenarians/centenarians[J].Exp Gerontol,2008,43(12):1108-1113.DOI:10.1016/j.exger.2008.08.046.
[5]中华医学会妇产科学分会妊娠期高血压疾病学组.妊娠期高血压疾病诊治指南(2015)[J].中华妇产科杂志,2015,50(10):721-728.DOI:10.3760/cma.j.issn.0529-567x.2015.10.001.
[6]Ganss R.Maternal Metabolism and Vascular Adaptation in Pregnancy:The PPAR Link[J].Trends Endocrinol Metab,2017,28(1):73-84.DOI:10.1016/j.tem.2016.09.004.
[7]Seber S,Ucak S,Basat O,et al.The effect of dual PPAR alpha/gamma stimulation with combination of rosiglitazone and fenofibrate on metabolic parameters in type 2 diabetic patients[J].Diabetes Res Clin Pract,2006,71(1):52-58.DOI:10.1016/j.diabres.2005.05.009.
[8]Oliver WR,Shenk JL,Snaith MR,et al.A selective peroxisome proliferator-activated receptor delta agonist promotes reverse cholesterol transport[J].Proc Natl Acad Sci U S A,2001,98(9):5306-5311.DOI:10.1073/pnas.091021198.
[9]蔺瑶,顾淑君,武鸣,等.过氧化物酶体增殖物激活受体的多个单核苷酸多态性与原发性高血压的关联研究[J].中华流行病学杂志,2012,33(6):597-601.DOI:10.3760/cma.j.issn.0254-6450.2012.06.012.
[10]丁一,郭志荣,武鸣,等.PPARD-87T>C与PPARA和PPARG单核苷酸多态性之间的交互作用对腹型肥胖的影响[J].中华医学杂志,2012,92(22):1517-1521.DOI:10.3760/cma.j.issn.0376-2491.2012.22.004.