丁香酚对长薄鳅幼鱼麻醉效果的研究
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摘要
【目的】为研究丁香酚对长薄鳅幼鱼的麻醉效果。【方法】在水温(22±1)℃条件下,以不同质量浓度的丁香酚对长薄鳅幼鱼的麻醉效果进行研究。【结果】20～120 mg/L质量浓度的丁香酚均能使长薄鳅进入麻醉期,且麻醉浓度与平均入麻时间呈负相关,与平均复苏时间呈正相关;丁香酚质量浓度在20～40 mg/L时,长薄鳅的呼吸频率在经历了先升后降的过程之后,呼吸频率趋于稳定,当增加至60～120 mg/L时,其呼吸频率经历小幅上升之后,均呈现直线下降趋势,并进入休克期;以60 mg/L丁香酚将长薄鳅麻醉后再在空气中进行暴露,暴露时间与复苏时间呈负相关,暴露时间在2～24 min范围内的复苏率为100%,72 h存活率均为100%,当时间增加至26 min时,复苏率降低至70%,72 h存活率仅为40%;采用10 mg/L丁香酚麻醉液或经60 mg/L的丁香酚麻醉后立即转入10 mg/L丁香酚麻醉液连续浸泡10 h,可使长薄鳅进入镇静期,且对试验鱼影响较小,推荐作为长薄鳅幼鱼长途运输模式。【结论】40～60 mg/L的丁香酚麻醉液是长薄鳅幼鱼的理想麻醉浓度。
【Objective】The present paper aimed to study the anesthetic effect of eugenol on Juvenile Elongateloach.【Method】Under the condition of water temperature at(22±1)℃, the effects of different concentrations of eugenol on juvenile Loach were studied. 【Result】The concentration of 20-120 mg/L of eugenol could make elongateloach into the anesthesia period, the anesthesia concentration and the average time into anesthesia was negatively correlated, the concentration of anesthesia and the average recovery time was positively correlated. When the concentration of eugenol was 20-40 mg/L, the respiration frequency tended to be stable after the process of respiration increased firstly and then decreased. When the concentration of eugenol increased to 60-120 mg/L, the respiratory rate of juvenile elongateloach had a downward trend after going through a slight increase and finally entered the shock period. The elongateloach were anesthetized with 60 mg/L eugenol and then exposed in air. The exposure time was negatively correlated with the recovery time. The recovery rate was 100 % at 2-24 min, and the survival rate after 72 hours were 100 %, when the time increased to 26 min, the recovery rate reduced to 70 % and 72 hours survival rate was only 40 %.Using the concentration of 10 mg/L of eugenol anesthesia or 60 mg/L of eugenol anesthesia immediately transferred to 10 mg/L of eugenol anesthesia continuous soak for 10 hours could make elongateloach into the sedation. The method had less influence on the test fish and meets the needs of long-distance transportation, which was recommended as a long-distance transport mode for the juvenile elongateloach. 【Conclusion】The 40-60 mg/L of eugenol anesthetic solution is the ideal anesthetic concentration for juveniles.
【Objective】The present paper aimed to study the anesthetic effect of eugenol on Juvenile Elongateloach.【Method】Under the condition of water temperature at(22±1)℃, the effects of different concentrations of eugenol on juvenile Loach were studied. 【Result】The concentration of 20-120 mg/L of eugenol could make elongateloach into the anesthesia period, the anesthesia concentration and the average time into anesthesia was negatively correlated, the concentration of anesthesia and the average recovery time was positively correlated. When the concentration of eugenol was 20-40 mg/L, the respiration frequency tended to be stable after the process of respiration increased firstly and then decreased. When the concentration of eugenol increased to 60-120 mg/L, the respiratory rate of juvenile elongateloach had a downward trend after going through a slight increase and finally entered the shock period. The elongateloach were anesthetized with 60 mg/L eugenol and then exposed in air. The exposure time was negatively correlated with the recovery time. The recovery rate was 100 % at 2-24 min, and the survival rate after 72 hours were 100 %, when the time increased to 26 min, the recovery rate reduced to 70 % and 72 hours survival rate was only 40 %.Using the concentration of 10 mg/L of eugenol anesthesia or 60 mg/L of eugenol anesthesia immediately transferred to 10 mg/L of eugenol anesthesia continuous soak for 10 hours could make elongateloach into the sedation. The method had less influence on the test fish and meets the needs of long-distance transportation, which was recommended as a long-distance transport mode for the juvenile elongateloach. 【Conclusion】The 40-60 mg/L of eugenol anesthetic solution is the ideal anesthetic concentration for juveniles.
引文
[1]汪松.中国濒危动物红皮书[M].北京:科学出版社, 2003.
[2]刘军.长江上游特有鱼类受威胁及优先保护顺序的定量分析[J].中国环境科学,2004,24(4):395-399.
[3]王志坚,殷江霞,张耀光.长薄鳅的精巢发育和精子发生[J].淡水渔业,2009,39(1):3-9.
[4]梁银铨,胡小建,虞功亮,等.长薄鳅仔稚鱼发育和生长的研究[J].水生生物学报,2004,28(1):96-100.
[5]李凤杰,曾令清,黄小铭,等.长薄鳅幼鱼消化道指数和消化酶活性研究[J].淡水渔业,2012,42(3):10-13.
[6]黄小铭,张耀光,江星,等.长薄鳅外周血细胞的显微结构和细胞化学特征研究[J].四川动物,2012,31(1):59-63.
[7]周剑,杜军,刘光迅,等.4种常用药物对长薄鳅幼鱼的急性毒性试验研究[J].西南农业学报,2012,25(5):1920-1924.
[8]Hoskonen P, Pirhonen J. Temperature effects on anaesthesia with clove oil in six temperate-zone fishes[J]. J Fish Biol, 2004,64:1136-1142.
[9]庄平,徐滨,章龙珍,等.MS-222和丁香酚对中华鲟幼鱼耗氧率与排氨率的影响[J].中国水产科学,2009,16(4):612-618.
[10]陈德芳,汪开毓,廖国平,等.丁香酚对斑马鱼麻醉效果的研究[J].水产科技情报,2013,40(4):193-198.
[11]梁政远,安丽娜,缪凌鸿,等.丁香油对鲤鱼和罗非鱼的麻醉作用[J].水产学杂志,2009,22(1):42-46.
[12]匡刚桥,李萍,郑曙明,等.丁香酚对斑点叉尾鮰幼鱼的麻醉效果[J].四川动物,2010,29(5):584-587.
[13]郭丰红,汪之和. MS-222 对鳜鱼成鱼麻醉效果的研究[J]. 湖南农业科学,2009(7):150-153.
[14]刘长琳,何力,陈四清,等. 鱼类麻醉研究综述[J]. 渔业现代化,2007,34(5): 21-25.
[15]Marking L L, M eyer F P. Are better anesthetics needed in fisheries[J]. Fisheries, 1985, 10: 2-5.
[16]李思发.鱼类麻醉剂[J].淡水渔业,1988(1):22-23.
[17]孟庆磊,张志山,张龙岗,等.丁香酚对苏氏圆魚芒腹成鱼麻醉效果的研究[J]. 长江大学学报(自然科学版),2012,9(4):33-36.
[18]徐开达,梁君,虞宝存,等. 丁香酚对日本黄姑鱼麻醉效果的研究[J]. 水产科技情报,2012,39(5): 234-237.
[19]赵明,柳学周,徐永江,等. MS-222麻醉圆斑星鲽成鱼效果研究[J]. 海洋科学进展,2010,28(4): 531-537.
[20]陈德芳,汪开毓,肖丹,等. 丁香酚对鲫鱼麻醉效果的研究[J]. 水产科技情报, 2010,37(6): 288-291.
[21]张富林,吕世明,韩俊伟. 丁香酚对鲤的麻醉作用[J]. 山地农业生物学报,2007,26(6): 504-507.
[22]关键,官曙光,郑永允,等. 2-苯氧乙醇和丁香酚对许氏平鮋幼鱼麻醉效果的实验研究[J]. 渔业科学进展, 2010,31(6): 22-28.
[23]张朝晖,丛娇日,王波,等. 麻醉剂丁香酚对黄腊鲹耗氧的影响[J]. 海洋科学, 2003,27(6): 11-14.
[24]魏锁成.用于鱼类的麻醉剂及麻醉管理[J].西北民族大学学报,2005,26(1):43-45.
[25]Cook S J, Suski C D, Ostrand K G,et al. Behavioral and physiological assessment of low concentration of clove oil anaesthetic for handing and transporting largemouth bass (Micropterus salmoides)[J].Aquaculture,2004,239:509-529.
[2]刘军.长江上游特有鱼类受威胁及优先保护顺序的定量分析[J].中国环境科学,2004,24(4):395-399.
[3]王志坚,殷江霞,张耀光.长薄鳅的精巢发育和精子发生[J].淡水渔业,2009,39(1):3-9.
[4]梁银铨,胡小建,虞功亮,等.长薄鳅仔稚鱼发育和生长的研究[J].水生生物学报,2004,28(1):96-100.
[5]李凤杰,曾令清,黄小铭,等.长薄鳅幼鱼消化道指数和消化酶活性研究[J].淡水渔业,2012,42(3):10-13.
[6]黄小铭,张耀光,江星,等.长薄鳅外周血细胞的显微结构和细胞化学特征研究[J].四川动物,2012,31(1):59-63.
[7]周剑,杜军,刘光迅,等.4种常用药物对长薄鳅幼鱼的急性毒性试验研究[J].西南农业学报,2012,25(5):1920-1924.
[8]Hoskonen P, Pirhonen J. Temperature effects on anaesthesia with clove oil in six temperate-zone fishes[J]. J Fish Biol, 2004,64:1136-1142.
[9]庄平,徐滨,章龙珍,等.MS-222和丁香酚对中华鲟幼鱼耗氧率与排氨率的影响[J].中国水产科学,2009,16(4):612-618.
[10]陈德芳,汪开毓,廖国平,等.丁香酚对斑马鱼麻醉效果的研究[J].水产科技情报,2013,40(4):193-198.
[11]梁政远,安丽娜,缪凌鸿,等.丁香油对鲤鱼和罗非鱼的麻醉作用[J].水产学杂志,2009,22(1):42-46.
[12]匡刚桥,李萍,郑曙明,等.丁香酚对斑点叉尾鮰幼鱼的麻醉效果[J].四川动物,2010,29(5):584-587.
[13]郭丰红,汪之和. MS-222 对鳜鱼成鱼麻醉效果的研究[J]. 湖南农业科学,2009(7):150-153.
[14]刘长琳,何力,陈四清,等. 鱼类麻醉研究综述[J]. 渔业现代化,2007,34(5): 21-25.
[15]Marking L L, M eyer F P. Are better anesthetics needed in fisheries[J]. Fisheries, 1985, 10: 2-5.
[16]李思发.鱼类麻醉剂[J].淡水渔业,1988(1):22-23.
[17]孟庆磊,张志山,张龙岗,等.丁香酚对苏氏圆魚芒腹成鱼麻醉效果的研究[J]. 长江大学学报(自然科学版),2012,9(4):33-36.
[18]徐开达,梁君,虞宝存,等. 丁香酚对日本黄姑鱼麻醉效果的研究[J]. 水产科技情报,2012,39(5): 234-237.
[19]赵明,柳学周,徐永江,等. MS-222麻醉圆斑星鲽成鱼效果研究[J]. 海洋科学进展,2010,28(4): 531-537.
[20]陈德芳,汪开毓,肖丹,等. 丁香酚对鲫鱼麻醉效果的研究[J]. 水产科技情报, 2010,37(6): 288-291.
[21]张富林,吕世明,韩俊伟. 丁香酚对鲤的麻醉作用[J]. 山地农业生物学报,2007,26(6): 504-507.
[22]关键,官曙光,郑永允,等. 2-苯氧乙醇和丁香酚对许氏平鮋幼鱼麻醉效果的实验研究[J]. 渔业科学进展, 2010,31(6): 22-28.
[23]张朝晖,丛娇日,王波,等. 麻醉剂丁香酚对黄腊鲹耗氧的影响[J]. 海洋科学, 2003,27(6): 11-14.
[24]魏锁成.用于鱼类的麻醉剂及麻醉管理[J].西北民族大学学报,2005,26(1):43-45.
[25]Cook S J, Suski C D, Ostrand K G,et al. Behavioral and physiological assessment of low concentration of clove oil anaesthetic for handing and transporting largemouth bass (Micropterus salmoides)[J].Aquaculture,2004,239:509-529.
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