材料表面浸润性对细菌粘附的影响
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摘要
材料表面的细菌粘附常引起食品腐败或植入性感染,有时甚至会引发疾病,而控制细菌在材料表面的初始粘附能够减少这些安全隐患。作者通过化学接枝的方法,制备了不同表面浸润性的材料,并与大肠杆菌、铜绿假单胞菌及金黄色葡萄球菌等3种常见致病菌共同培养,系统地研究了材料表面浸润性对细菌粘附的影响。研究结果表明,随着材料表面疏水性的增加,其抗细菌粘附能力显著提升。另外,表面带负电材料的抗菌能力更强。这些结果能够帮助理解细菌在材料表面粘附的内在机理,同时有助于抗菌材料的设计和制备。
Bacteria adhesion on surfaces often causes implanted infections,food spoilage and human illness. The control of the initial adhesion to materials is very important to avoid safety and health problems. In this study,materials with different surface wettability were fabricated via chemical grafting. Three model strains(Escherichia coli BL21,Staphylococcus aureus 6538 and Pseudomonas aeruginosa ATCC 9027) were imported to demonstrate the influence of surface wettability markedly.Besides,surfaces with negative charge were more resistant to bacterial adhesion. The results of this study provided new insights into the mechanism of bacteria adhesion,and could be effectively used in designing of anti-bacterial materials.
Bacteria adhesion on surfaces often causes implanted infections,food spoilage and human illness. The control of the initial adhesion to materials is very important to avoid safety and health problems. In this study,materials with different surface wettability were fabricated via chemical grafting. Three model strains(Escherichia coli BL21,Staphylococcus aureus 6538 and Pseudomonas aeruginosa ATCC 9027) were imported to demonstrate the influence of surface wettability markedly.Besides,surfaces with negative charge were more resistant to bacterial adhesion. The results of this study provided new insights into the mechanism of bacteria adhesion,and could be effectively used in designing of anti-bacterial materials.
引文
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[2]RENNER L D,WEIBEL D B.Physicochemical regulation of biofilm formation[J].MRS Bulletin,2011,36(5):347?355.
[3]HOUARI A,DI M P.Effect of chlorhexidine and benzalkonium chloride on bacterial biofilm formation[J].Letters in Applied Microbiology,2007,45(6):652-656.
[4]SONG F,KOO H,REN D.Effects of material properties on bacterial adhesion and biofilm formation[J].Journal of Dental Research,2015,94(8):1027-1034.
[5]MENG J,ZHANG P,WANG S.Recent progress in biointerfaces with controlled bacterial adhesion by using chemical and physical methods[J].Chemistry-An Asian Journal,2014,9(8):2004-2016.
[6]CAO Z,MI L,MENDIOLA J,et al.Reversibly switching the function of a surface between attacking and defending against bacteria[J].Angewandte Chemie International Edition,2012,51(11):2602-2605.
[7]KINGSHOTT P,WEI J,BAGGE R D,et al.Covalent attachment of poly(ethylene glycol)to surfaces,critical for reducing bacterial adhesion[J].Langmuir,2003,19(17):6912-6921.
[8]SATOU N,SATOU J,SHINTANI H,et al.Adherence of streptococci to surface-modified glass[J].Journal of General Microbiology,1988,134(5):1299-1305.
[9]MORRA M,CASSINELI C.Non-fouling properties of polysaccharide-coated surfaces[J].Journal of Biomaterials Science,Polymer Edition,1999,10(10):1107-1124.
[10]ZHOU Kun,JIANG Zhongying.Electrochemical behaviors of hybrid self-assembledmonolayers modified electrode[J].Journal of Soochow University:Natural Science Edition,2011,27(4):47-52.(in Chinese)
[11]FRIEDLANDER R S,VLAMAKIS H,KIM P,et al.Bacterial flagella explore microscale hummocks and hollows to increase adhesion[J].Proceedings of the National Academy of Sciences,2013,110(14):5624-5629.
[12]AHN J,SOHN E H,BANG S H,et al.Biocompatible Ag nanoparticle-embedded poly(2-hydroxyethyl methacrylate)derivative films with bacterial adhesion-resistant and antibacterial properties[J].Macromolecular Research,2014,22(3):337-343.
[13]HUANG Jianhua,JIANG Wenhua,HAN Shijun.Mon te carlo study on conformation properties of tail-like chain of block copolymer[J].Chemical Journal of Chinese Universities,2004,25(1):179-183.(in Chinese)
[14]WU Rangquan,LI Fanting,CHENG Zhengdi.Randomized studies excluded volume effect of the polymer chain[J].Chinese Science Bulletin,1981,12:23.(in Chinese)
[15]HUANG Baowei,ZHANG Hongmei,LIU Xuelu,et.al.Different patterns of biofilm formation and effect of Sub-MIC disinfectant on biofilm formation of foodbore pathogen[J].Journal of Food Science and Biotechnology,2011,30(5):757-760.(in Chinese)
[16]ARIMA Y,IWATA H.Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers[J].Biomaterials,2007,28(20):3074-3082.
[17]LEE J H,KHANG G,LEE J W,et al.Interaction of different types of cells of polymer surfaces with wettability gradient[J].JColloid Interface Sci,1998,205(2):323-330.
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