四种常见离体脱位牙贮存介质对其牙周膜成纤维细胞生物活性的影响
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摘要
目的:评价比较四种常见离体脱位牙贮存介质对牙周膜成纤维细胞活性的影响。方法:选取2017年1月-2018年1月本院口腔临床14～22岁青少年200例因正畸需要拔除的下颌第一前磨牙200颗作为研究对象,将其随机分为四组试验组(生理盐水组、牛奶组、唾液组、自来水组)和对照组,各40颗。培养脱位牙中的牙周膜成纤维细胞,其中试验分别采用生理盐水、牛奶、唾液、自来水作为贮存介质,对照组则直接在空气中保存。采用CCK-8法分别检测牙周膜细胞在四种常见离体脱位牙贮存介质中2、6、12、24 h不同时间点的活性,并与对照组进行比较。结果:各试验组不同时间点的牙周膜细胞存活率均明显高于对照组,差异均有统计学意义(P<0.05)。生理盐水、牛奶及唾液组不同时间点的牙周膜细胞存活率均明显高于自来水组,差异均有统计学意义(P<0.05)。牛奶、唾液组不同时间点的牙周膜细胞存活率均明显高于生理盐水,且牛奶组12、24 h的牙周膜细胞存活率均明显高于唾液组,差异均有统计学意义(P<0.05)。结论:牛奶、唾液贮存的牙周膜成纤维细胞活性较高,可作为有效的离体脱位牙临时贮存液,而生理盐水的保存效果次之,而自来水保存效果最差,不适合作为贮存介质。
Objective:To evaluate the effect of four common in vitro dislocated teeth storage medium on the biological activity of periodontal ligament fibroblasts.Method:A total of 200 mandibular first premolars extracted from 200 cases of 14-22 year old adolescents in our hospital due to orthodontic treatment from January 2017 to January 2018 were selected as the research objects.They were randomly divided into four experimental groups(saline group,milk group,saliva group,tap water group)and control group,40 teeth in each group.The periodontal ligament fibroblasts were cultured in the dislocation teeth.The experimental group were treated with saline,milk,saliva and tap water as storage medium respectively,while the control group was stored directly in air.The periodontal ligament cells were detected by CCK-8 method in the storage medium of four common in vitro dislocation teeth for 2,6,12 and 24 h respectively.The activity at different time points were compared with the control group.Result:The survival rate of periodontal ligament cells in each experimental group at different time points were significantly higher than those in the control group,the differences were statistically significant(P<0.05).The survival rate of periodontal ligament cells in the saline,milk and saliva groups at different time points were significantly higher than those in the tap water group,the differences were statistically significant(P<0.05).The survival rate of periodontal ligament cells in the milk and saliva groups at different time points were significantly higher than those in the saline group,and the survival rate of periodontal ligament cells in the milk group at 12 and 24 h was significantly higher than that in the saliva group,the differences were statistically significant(P<0.05).Conclusion:The periodontal ligament fibroblasts stored in milk and saliva have higher activity and can be used as effective temporary storage solution for dislocated teeth in vitro.The storage effect of saline is the second,while that of tap water is the worst,and it is not suitable for storage medium.
Objective:To evaluate the effect of four common in vitro dislocated teeth storage medium on the biological activity of periodontal ligament fibroblasts.Method:A total of 200 mandibular first premolars extracted from 200 cases of 14-22 year old adolescents in our hospital due to orthodontic treatment from January 2017 to January 2018 were selected as the research objects.They were randomly divided into four experimental groups(saline group,milk group,saliva group,tap water group)and control group,40 teeth in each group.The periodontal ligament fibroblasts were cultured in the dislocation teeth.The experimental group were treated with saline,milk,saliva and tap water as storage medium respectively,while the control group was stored directly in air.The periodontal ligament cells were detected by CCK-8 method in the storage medium of four common in vitro dislocation teeth for 2,6,12 and 24 h respectively.The activity at different time points were compared with the control group.Result:The survival rate of periodontal ligament cells in each experimental group at different time points were significantly higher than those in the control group,the differences were statistically significant(P<0.05).The survival rate of periodontal ligament cells in the saline,milk and saliva groups at different time points were significantly higher than those in the tap water group,the differences were statistically significant(P<0.05).The survival rate of periodontal ligament cells in the milk and saliva groups at different time points were significantly higher than those in the saline group,and the survival rate of periodontal ligament cells in the milk group at 12 and 24 h was significantly higher than that in the saliva group,the differences were statistically significant(P<0.05).Conclusion:The periodontal ligament fibroblasts stored in milk and saliva have higher activity and can be used as effective temporary storage solution for dislocated teeth in vitro.The storage effect of saline is the second,while that of tap water is the worst,and it is not suitable for storage medium.
引文
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[3]杨洋,徐燕,孟明理,等.牙龈卟啉单胞菌对牙周膜成纤维细胞活性、炎性因子与骨代谢基因表达的影响研究[J].安徽医科大学学报,2016,51(6):786-790.
[4]李鑫,李斯翰,匡威,等.改良HBSS液对体外培养人牙周膜细胞存活和克隆能力的影响[J].现代口腔医学杂志,2013,27(6):352-355.
[5]李玮,李坤阳,范云,等.富血小板纤维蛋白对人牙周膜成纤维细胞迁移及成骨分化的影响[J].郑州大学学报(医学版),2014,49(1):108-112.
[6]韩亚琨.绿茶提取物对牙周膜细胞活性影响的体外研究[J].吉林医药学院学报,2017,38(4):241-243.
[7]薛阳,高洁.不同处理液对脱位牙牙周膜修复作用的实验研究[J].口腔医学,2015,35(10):813-817.
[8]张旸,汪俊.全脱位牙牙周膜细胞活性在牛奶中的保存及其影响因素[J].国际口腔医学杂志,2015,42(1):84-88.
[9]Cobankara K F,Ungor M.Replantation after extended dry storage of avulsed permanent incisors:report of a case[J].Dent Traumatol,2007,23(4):251-256.
[10]蔡文姬,王佐林.牙周膜对于牙槽骨吸收影响的初步研究[J].口腔颌面外科杂志,2016,26(5):311-316.
[11]李鑫,段建民.脱位牙体外保存介质的研究进展[J].国际口腔医学杂志,2013,40(3):381-384.
[12]闫瑞欣,陈丹,闫奇,等.缺氧环境对牙周膜成纤维细胞增殖及凋亡的影响[J].北京口腔医学,2016,24(3):146-149.
[13]周扬.日常介质保存脱位牙细胞活性的比较研究[D].大连:大连医科大学,2017:38-39.
[14]葛艳丽.外伤致年轻恒牙脱位后再植的临床疗效及其影响因素[J].中国医学工程,2015,21(1):76-77.
[15]祝策,陆海霞,冯希平.辅助治疗方法在年轻恒牙再植术中的应用[J].牙体牙髓牙周病学杂志,2014,24(9):554-557.
[16]化萌萌,王亚宇,郭泾.透明质酸对尼古丁作用下人牙周膜成纤维细胞增殖影响研究[J].口腔医学,2018,38(4):314-319.
[17]储雯,王冰,张一诺,等.云南白药对体外培养人牙周膜成纤维细胞C-fos、C-jun基因表达的影响[J].昆明医科大学学报,2016,37(9):5-8.
[18]刘勇,周三玲,高黎,等.不同离体时间和保存液对犬离体牙牙周膜细胞活力影响的实验研究[J].牙体牙髓牙周病学杂志,2012,22(1):21-24.
[19]陈晖,张旸,汪俊,等.全脱位牙延期再植根面牙周膜不同去除方法的比较[J].上海口腔医学,2016,25(3):296-300.
[2]陈富波,祝春铁,李宏权,等.六种保存液对牙周膜成纤维细胞活性的影响[J].口腔材料器械杂志,2012,21(4):203-206.
[3]杨洋,徐燕,孟明理,等.牙龈卟啉单胞菌对牙周膜成纤维细胞活性、炎性因子与骨代谢基因表达的影响研究[J].安徽医科大学学报,2016,51(6):786-790.
[4]李鑫,李斯翰,匡威,等.改良HBSS液对体外培养人牙周膜细胞存活和克隆能力的影响[J].现代口腔医学杂志,2013,27(6):352-355.
[5]李玮,李坤阳,范云,等.富血小板纤维蛋白对人牙周膜成纤维细胞迁移及成骨分化的影响[J].郑州大学学报(医学版),2014,49(1):108-112.
[6]韩亚琨.绿茶提取物对牙周膜细胞活性影响的体外研究[J].吉林医药学院学报,2017,38(4):241-243.
[7]薛阳,高洁.不同处理液对脱位牙牙周膜修复作用的实验研究[J].口腔医学,2015,35(10):813-817.
[8]张旸,汪俊.全脱位牙牙周膜细胞活性在牛奶中的保存及其影响因素[J].国际口腔医学杂志,2015,42(1):84-88.
[9]Cobankara K F,Ungor M.Replantation after extended dry storage of avulsed permanent incisors:report of a case[J].Dent Traumatol,2007,23(4):251-256.
[10]蔡文姬,王佐林.牙周膜对于牙槽骨吸收影响的初步研究[J].口腔颌面外科杂志,2016,26(5):311-316.
[11]李鑫,段建民.脱位牙体外保存介质的研究进展[J].国际口腔医学杂志,2013,40(3):381-384.
[12]闫瑞欣,陈丹,闫奇,等.缺氧环境对牙周膜成纤维细胞增殖及凋亡的影响[J].北京口腔医学,2016,24(3):146-149.
[13]周扬.日常介质保存脱位牙细胞活性的比较研究[D].大连:大连医科大学,2017:38-39.
[14]葛艳丽.外伤致年轻恒牙脱位后再植的临床疗效及其影响因素[J].中国医学工程,2015,21(1):76-77.
[15]祝策,陆海霞,冯希平.辅助治疗方法在年轻恒牙再植术中的应用[J].牙体牙髓牙周病学杂志,2014,24(9):554-557.
[16]化萌萌,王亚宇,郭泾.透明质酸对尼古丁作用下人牙周膜成纤维细胞增殖影响研究[J].口腔医学,2018,38(4):314-319.
[17]储雯,王冰,张一诺,等.云南白药对体外培养人牙周膜成纤维细胞C-fos、C-jun基因表达的影响[J].昆明医科大学学报,2016,37(9):5-8.
[18]刘勇,周三玲,高黎,等.不同离体时间和保存液对犬离体牙牙周膜细胞活力影响的实验研究[J].牙体牙髓牙周病学杂志,2012,22(1):21-24.
[19]陈晖,张旸,汪俊,等.全脱位牙延期再植根面牙周膜不同去除方法的比较[J].上海口腔医学,2016,25(3):296-300.