miR-24-3p在胰腺癌中的表达及意义
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摘要
目的:分析57例胰腺癌患者的癌组织与癌旁组织中的miR-24-3p的表达情况及临床意义。方法:收集57例胰腺癌初诊患者术后的癌组织及癌旁组织,用实时荧光定量PCR技术检测miR-24-3p在胰腺癌组织和相应癌旁组织中的表达情况,用χ2检验分析miR-24-3p的表达与胰腺癌患者临床病理指标之间的相关性;用Kaplan-Meier单因素分析计算累积生存率和Log-rank检验分析胰腺癌组织中miR-24-3p的表达量与胰腺癌患者术后预后的关系。结果:miR-24-3p在胰腺癌组织中相对高表达; miR-24-3p的表达与患者的年龄、性别、肿瘤分化程度、肿瘤位置和血管浸润无关(P> 0. 05),而与肿瘤的大小、TNM分期和淋巴结转移相关(P <0. 05); miR-24-3p高表达组患者的3年累计生存率明显低于miR-24-3p低表达组患者(P <0. 05)。结论:miR-24-3p在胰腺癌组织中的表达明显高于其癌旁组织,提示其参与了胰腺癌的发生发展过程。
Objective:To investigate the expression of miR-24-3 p and its clinical significance in pancreatic cancer.Methods:The expression of miR-24-3 p in 57 pairs of fresh frozen pancreatic ductal adenocarcinoma(PDAC) specimens and their corresponding adjacent non-cancerous pancreas specimens was detected by quantitative real-time PCR(qRT-PCR).Then,the patients were divided into high and low expression groups.The correlation between the expression of miR-24-3 p and the clinicopathological characteristics was analyzed by Chi-square test.Survival curves were plotted using the Kaplan-Meier method,then univariate analysis of all variables was made using the Log-rank test.Results:The expression of miR-24-3 p in PDAC tissue was up-regulated compared to their corresponding adjacent non-cancerous tissue(P<0.05).The expression of miR-24-3 p was not correlated with age,gender,tumor differentiation,tumor location and vascular invasion(P>0.05).However,miR-24-3 p expression was positively associated with tumor size,TNM stage,lymph node metastasis.And the prognosis of miR-24-3 p high expression group was poor than miR-24-3 p low expression group.Conclusion:miR-24-3 p was significantly up-regulated in PDAC tissue.It might contribute to the development and progression of PDAC.
Objective:To investigate the expression of miR-24-3 p and its clinical significance in pancreatic cancer.Methods:The expression of miR-24-3 p in 57 pairs of fresh frozen pancreatic ductal adenocarcinoma(PDAC) specimens and their corresponding adjacent non-cancerous pancreas specimens was detected by quantitative real-time PCR(qRT-PCR).Then,the patients were divided into high and low expression groups.The correlation between the expression of miR-24-3 p and the clinicopathological characteristics was analyzed by Chi-square test.Survival curves were plotted using the Kaplan-Meier method,then univariate analysis of all variables was made using the Log-rank test.Results:The expression of miR-24-3 p in PDAC tissue was up-regulated compared to their corresponding adjacent non-cancerous tissue(P<0.05).The expression of miR-24-3 p was not correlated with age,gender,tumor differentiation,tumor location and vascular invasion(P>0.05).However,miR-24-3 p expression was positively associated with tumor size,TNM stage,lymph node metastasis.And the prognosis of miR-24-3 p high expression group was poor than miR-24-3 p low expression group.Conclusion:miR-24-3 p was significantly up-regulated in PDAC tissue.It might contribute to the development and progression of PDAC.
引文
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[6] Tétreault N,Guire VD.miRNAs:Their discovery,biogenesis and mechanism of action[J].Clinical Biochemistry,2013,46(10-11):842-845.
[7] Sun X,Xiao D,Xu T,et al.miRNA-24-3p promotes cell proliferation and regulates chemosensitivity in head and neck squamous cell carcinoma by targeting CHD5[J].Future Oncol,2016,12(23):2701-2712.
[8] Yu G,Jia Z,Dou Z.miR-24-3p regulates bladder cancer cell proliferation,migration,invasion and autophagy by targeting DEDD[J].Oncology Reports,2017,37(2):1123-1131.
[9] Zhu G,Zhou L,Liu H,et al.MicroRNA-224 promotes pancreatic cancer cell proliferation and migration by targeting the TXNIP-mediated HIF1alpha pathway[J].Cell Physiol Biochem,2018,48(4):1735-1746.
[10] Yang Y,Tao X,Li CB,et al.MicroRNA-494 acts as a tumor suppressor in pancreatic cancer,inhibiting epithelial-mesenchymal transition,migration and invasion by binding to SDC1[J].Int J Oncol,2018,53(3):1204-1214.
[11] Srivastava N,Manvati S,Srivastava A,et al.miR-24-2 controls H2AFX expression regardless of gene copy number alteration and induces apoptosis by targeting antiapoptotic gene BCL-2:A potential for therapeutic intervention[J].Breast Cancer Res,2011,13(2):R39.
[12] Pass HI,Beer DG,Joseph S,et al.Biomarkers and molecular testing for early detection,diagnosis,and therapeutic prediction of lung cancer[J].Thoracic Surgery Clinics,2013,23(2):211-224.
[13] Dillhoff M,Liu J,Frankel W,et al.MicroRNA-21 is overexpressed in pancreatic cancer and a potential predictor of survival[J].J Gastrointest Surg,2008,12(12):2171-2176.
[14] Ali S,Almhanna K,Chen W,et al.Differentially expressed miRNAs in the plasma may provide a molecular signature for aggressive pancreatic cancer[J].Am J Transl Res,2010,3(1):28-47.
[15] Kawaguchi T,Komatsu S,Ichikawa D,et al.Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer[J].Br J Cancer,2013,108(2):361-369.
[16] Quan Y,Huang X,Quan X.Expression of miRNA-206 and miRNA-145 in breast cancer and correlation with prognosis[J].Oncol Lett,2018,16(5):6638-6642.
[17] Khodadadi-Jamayran A,Akgol-Oksuz B,Afanasyeva Y,et al.Prognostic role of elevated mir-24-3p in breast cancer and its association with the metastatic process[J].Oncotarget,2018,9(16):12868-12878.
[18] Ye SB,Zhang H,Cai TT,et al.Exosomal miR-24-3p impedes T-cell function by targeting FGF11 and serves as a potential prognostic biomarker for nasopharyngeal carcinoma[J].J Pathol,2016,240(3):329-340.
[19] Yu L,Xiang L,Feng J,et al.miRNA-21 and miRNA-223 expression signature as a predictor for lymph node metastasis,distant metastasis and survival in kidney renal clear cell carcinoma[J].J Cancer,2018,9(20):3651-3659.
[20] Li Z,Tao Y,Wang X,et al.Tumor-secreted exosomal miR-222 promotes tumor progression via regulating p27 expression and re-localization in pancreatic cancer[J].Cell Physiol Biochem,2018,51(2):610-629.