评价两种消毒剂对IVF实验室微环境和妊娠结局的影响
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摘要
目的在辅助生殖体外受精(in vitro fertilization,IVF)实验室的胚胎操作过程中,根据消毒剂对胚胎质量和妊娠结局的影响,评价季铵盐消毒剂是否比75%酒精消毒液更适合用于IVF实验室。方法回顾分析2016年1月至9月在我中心行IVF的619例新鲜移植周期,根据IVF实验室使用的消毒剂不同分为两组,A组383例为使用75%酒精消毒组,B组236例使用季铵盐组。对比两组的VOC浓度、获卵数、获卵率、受精率、多精率、卵裂率、优质胚胎率、优质囊胚形成率、着床率、临床妊娠率以及流产率。结果相比于A组,B组的VOC浓度显著降低;A、B两组的获卵数、获卵率、受精率、多精率、卵裂率、优质胚胎率、优质囊胚形成率和流产率的差异均不具有统计学意义(P>0.05);但B组的着床率和妊娠率显著高于A组(P<0.05)。结论两组对胚胎的体外培养质量没有显著性的影响,但使用季铵盐组相比于75%酒精组胚胎着床率和妊娠率显著提高。因此,季铵盐消毒剂比75%酒精更适合作为IVF实验室消毒剂。
Objective To evaluate the influence of two disinfectants,quaternary ammonium salt vs 75% alcohol,on IVF laboratory microenvironment and pregnancy outcome and determine which one is more suitable for in vitro fertilization-embryo transfer(IVF-ET)operation.Methods The clinical and laboratory data of 619 patients in our center from January 2016 to September 2016 were respectively analyzed.The patients were divided into group A(383 cases with 75% alcohol as the disinfectant)and group B(236 cases with quaternary ammonium salt as the disinfectant).Then the concentration of volatile organic compounds(VOCs),oocyte number,oocyte retrieval rate,fertilization rate,polyspermic fertilization rate(≥3PN),cleavage rate,top quality Day-3 embryo rate,good-quality blastocyst formation rate,implantation rate,pregnancy rate and abortion rate were compared between the groups.Results Compared with group A,the VOC concentration in group B reduced significantly,while the implantation rate and pregnancy rate were higher significantly(P<0.05).There were no significant differences in the other indicators observed(P>0.05).Conclusion The two kinds of disinfectants have no significant influence on the embryo quality,but quaternary ammonium salt is superior to 75% alcohol in improving the implantation rate and pregnancy rate,thus is more suitable for IVF laboratory disinfection during IVF-ET operation.
Objective To evaluate the influence of two disinfectants,quaternary ammonium salt vs 75% alcohol,on IVF laboratory microenvironment and pregnancy outcome and determine which one is more suitable for in vitro fertilization-embryo transfer(IVF-ET)operation.Methods The clinical and laboratory data of 619 patients in our center from January 2016 to September 2016 were respectively analyzed.The patients were divided into group A(383 cases with 75% alcohol as the disinfectant)and group B(236 cases with quaternary ammonium salt as the disinfectant).Then the concentration of volatile organic compounds(VOCs),oocyte number,oocyte retrieval rate,fertilization rate,polyspermic fertilization rate(≥3PN),cleavage rate,top quality Day-3 embryo rate,good-quality blastocyst formation rate,implantation rate,pregnancy rate and abortion rate were compared between the groups.Results Compared with group A,the VOC concentration in group B reduced significantly,while the implantation rate and pregnancy rate were higher significantly(P<0.05).There were no significant differences in the other indicators observed(P>0.05).Conclusion The two kinds of disinfectants have no significant influence on the embryo quality,but quaternary ammonium salt is superior to 75% alcohol in improving the implantation rate and pregnancy rate,thus is more suitable for IVF laboratory disinfection during IVF-ET operation.
引文
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[2]Deo AA,Kulkarni AS,Meshram SU.Monocationic surfactant induced ultra structural changes in antibiotic resistant Escherichia coli[J].Indian J Med Res,2010,131:825-828.
[3]Ioannou CJ,Hanlon GW,Denyer SP.Action of disinfectant quaternary ammonium compounds against Staphylococcus aureus[J].Antimicrob Agents Chemother,2007,51(1):296-306.
[4]Buffetbataillon S,Tattevin P,Bonnauremallet M,et al.Emergence of resistance to antibacterial agents:the role of quaternary ammonium compounds—a critical review[J].Int J Antimicrob Agents,2012,39(5):381-389.
[5]Fuller BJ.Cryoprotectants:the essential antifreezes to protect life in the frozen state[J].Cryo Letters,2004,25(6):375-388.
[6]Hiraoka K,Fuchiwaki M,Horiuchi T,et al.Vitrified human day-7blastocyst transfer:11cases[J].Reprod Biomed Online,2008,17(5):689-694.
[7]Wennerholm UB.Cryopreservation of embryos and oocytes:obstetric outcome and health in children[J].Hum Reprod,2000,15(suppl 5):18-25.
[8]Brown SK.Chamber assessment of formaldehyde and VOC emissions from wood-based panels[J].Indoor Air,1999,9(3):209-215.
[9]Cohen J,Gilligan A,Esposito W,et al.Ambient air and its potential effects on conception in vitro[J].Hum Reprod,1997,12(8):1742-1749.
[10]Hall J,Gilligan A,Schimmel T,et al.The origin,effects and control of air pollution in laboratories used for human embryo culture[J].Hum Reprod,1999,13(Suppl 4):146-155.