白藜芦醇对人肺腺癌细胞A549增殖及迁移的抑制作用
|
摘要
目的探讨白藜芦醇对人肺腺癌细胞A549增殖及迁移的抑制作用。方法取对数生长期A549细胞,分别应用0、10、30、50、100、300及500μmol/L的白藜芦醇进行干预,24 h、48 h及72 h后检测各组人肺腺癌细胞A549的增殖抑制率。分别应用0、10、30、50、100、300μmol/L白藜芦醇干预A549细胞,24 h、48 h、72 h后测定各组细胞迁移能力。结果在10~100μmol/L浓度范围内,相同作用时间下A549细胞增殖抑制率随着白藜芦醇浓度的增加而提高,且在相同的白藜芦醇浓度下A549细胞增殖抑制率随着白藜芦醇作用时间延长而提高(P<0.05)。在大于100μmol/L浓度范围时,相同作用时间下不同浓度白藜芦醇对人肺腺癌A549细胞增殖抑制率比较,差异无统计学意义(P>0.05),但同一浓度下A549细胞增殖抑制率随着白藜芦醇作用时间延长而提高(P<0.05)。相同作用时间下10~300μmol/L白藜芦醇浓度组细胞的划痕宽度较0μmol/L白藜芦醇组增大,且10~300μmol/L白藜芦醇浓度组的划痕宽度随作用时间延长而增大(P<0.05)。结论白藜芦醇对人肺腺癌A549细胞增殖及迁移有较好的抑制作用,在一定的浓度范围内抑制率呈时间和剂量依赖性。
Objective To study the inhibitory effect of resveratrol on the proliferation and migration of human lung adenocarcinoma A549 cells. Methods The A549 cells at the logarithmic phase were treated with 0,10,30,50,100,300 and 500 μmol/L resveratrol respectively,then after 24 h,48 h and 72 h,the inhibitory rates of human lung adenocarcinoma A549 cell proliferation were determined in each group. And at 24 h,48 h and 72 h after A549 cells were treated by 0,10,30,50,100 and 300 μmol/L resveratrol respectively,the migration capability of the cells was determined in each group. Results The inhibitory rate of A549 cell proliferation increased with the increasing concentration of resveratrol,ranged 10 to 100 μmol/L,when treated with the equivalent treatment time,and the inhibitory rate of A549 cell proliferation increased with the increasing treatment time of resveratrol when treated by the equivalent concentration of resveratrol( P < 0. 05). No statistically significant difference was found in the inhibitory rate of human lung adenocarcinoma A549 cell proliferation among different concentrations of resveratrol,ranged over 100 μmol/L,in case of the equivalent treatment time( P > 0. 05),but the inhibitory rate of A549 cell proliferation increased with the increasing treatment time of resveratrol when treated by the equivalent concentration( P < 0. 05). The scratch width of the cells increased in the 10 μmol/L,30 μmol/L,50 μmol/L,100 μmol/L and 300 μmol/L resveratrol groups compared to the width in the 0 μmol/L resveratrol group in case of the equivalent treatment time( P < 0. 05),and the scratch width increased with the increasing treatment time in the 10 μmol/L,30 μmol/L,50 μmol/L,100 μmol/L and 300 μmol/L resveratrol groups( P < 0. 05). Conclusion Resveratrol of a certain concentration limits has better inhibitory effects on the proliferation and migration of human lung adenocarcinoma A549 cells in a time-dose dependent manner.
Objective To study the inhibitory effect of resveratrol on the proliferation and migration of human lung adenocarcinoma A549 cells. Methods The A549 cells at the logarithmic phase were treated with 0,10,30,50,100,300 and 500 μmol/L resveratrol respectively,then after 24 h,48 h and 72 h,the inhibitory rates of human lung adenocarcinoma A549 cell proliferation were determined in each group. And at 24 h,48 h and 72 h after A549 cells were treated by 0,10,30,50,100 and 300 μmol/L resveratrol respectively,the migration capability of the cells was determined in each group. Results The inhibitory rate of A549 cell proliferation increased with the increasing concentration of resveratrol,ranged 10 to 100 μmol/L,when treated with the equivalent treatment time,and the inhibitory rate of A549 cell proliferation increased with the increasing treatment time of resveratrol when treated by the equivalent concentration of resveratrol( P < 0. 05). No statistically significant difference was found in the inhibitory rate of human lung adenocarcinoma A549 cell proliferation among different concentrations of resveratrol,ranged over 100 μmol/L,in case of the equivalent treatment time( P > 0. 05),but the inhibitory rate of A549 cell proliferation increased with the increasing treatment time of resveratrol when treated by the equivalent concentration( P < 0. 05). The scratch width of the cells increased in the 10 μmol/L,30 μmol/L,50 μmol/L,100 μmol/L and 300 μmol/L resveratrol groups compared to the width in the 0 μmol/L resveratrol group in case of the equivalent treatment time( P < 0. 05),and the scratch width increased with the increasing treatment time in the 10 μmol/L,30 μmol/L,50 μmol/L,100 μmol/L and 300 μmol/L resveratrol groups( P < 0. 05). Conclusion Resveratrol of a certain concentration limits has better inhibitory effects on the proliferation and migration of human lung adenocarcinoma A549 cells in a time-dose dependent manner.
引文
[1]Ferlay J,Soerjomataram I,Dikshit R,et al.Cancer incidence and mortality worldwide:sources,methods and major patterns in GLOBOCAN 2012[J].Int J Cancer,2015,136(5):E359-E386.
[2]Jemal A,Center MM,De Santis C,et al.Global patterns of cancer incidence and mortality rates and trends[J].Cancer Epidemiol Biomarkers Prev,2010,19(8):1 893-1 907.
[3]Vallières E.Role of adjuvant systemic therapy for stage I NSCLC[J].Thorac Surg Clin,2007,17(2):279-285.
[4]Chen B,Zheng J,Zeng Y,et al.Sequence-dependent antiproliferative effects of gefitinib and docetaxel on non-small cell lung cancer(NSCLC)cells and the possible mechanism[J].PLo S One,2014,9(12):e114074.
[5]陈海霞,杨耀琴,陶惠红,等.白藜芦醇对肺腺癌A549细胞增殖、黏附与侵袭的影响[J].中国肿瘤生物治疗杂志,2012,19(2):138-142.
[6]Bertelli AA,Baccalini R,Battaglia E,et al.Resveratrol inhibits TNF alpha-induced endothelial cell activation[J].Therapie,2001,56(5):613-616.
[7]刘焕义,赵煜,张小龙,等.白藜芦醇诱导肺腺癌A549细胞凋亡的机制[J].中国老年学杂志,2014,34(3):714-715.
[8]Garvin S,Ollinger K,Dabrosin C.Resveratrol induces apoptosis and inhibits angiogenesis in human breast cancer xenografts in vivo[J].Cancer Lett,2006,231(1):113-122.
[9]Saiko P,Szakmary A,Jaeger W,et al.Resveratrol and its analogs:defense against cancer,coronary disease and neurodegenerative maladies or just a fad?[J].Mutat Res,2008,658(1/2):68-94.
[10]Weng CJ,Yang YT,Ho CT,et al.Mechanisms of apoptotic effects induced by resveratrol,dibenzoylmethane,and their analogues on human lung carcinoma cells[J].J Agric Food Chem,2009,57(12):5 235-5 243.
[11]Zhou R,Fukui M,Choi HJ,et al.Induction of a reversible,non-cytotoxic S-phase delay by resveratrol:implications for a mechanism of lifespan prolongation and cancer protection[J].Br J Pharmacol,2009,158(2):462-474.
[12]Vanamala J,Reddivari L,Radhakrishnan S,et al.Resveratrol suppresses IGF-1 induced human colon cancer cell proliferation and elevates apoptosis via suppression of IGF-1R/Wnt and activation of p53 signaling pathways[J].BMC Cancer,2010,10:238.
[13]Hudson TS,Hartle DK,Hursting SD,et al.Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms[J].Cancer Res,2007,67(17):8 396-8 405.
[14]于柏艳,孙抒,杨万山,等.虎杖提取物对人肺癌A549细胞株抑制增殖和诱导凋亡作用的研究[J].中成药,2010,32(11):1 972-1 975.
[2]Jemal A,Center MM,De Santis C,et al.Global patterns of cancer incidence and mortality rates and trends[J].Cancer Epidemiol Biomarkers Prev,2010,19(8):1 893-1 907.
[3]Vallières E.Role of adjuvant systemic therapy for stage I NSCLC[J].Thorac Surg Clin,2007,17(2):279-285.
[4]Chen B,Zheng J,Zeng Y,et al.Sequence-dependent antiproliferative effects of gefitinib and docetaxel on non-small cell lung cancer(NSCLC)cells and the possible mechanism[J].PLo S One,2014,9(12):e114074.
[5]陈海霞,杨耀琴,陶惠红,等.白藜芦醇对肺腺癌A549细胞增殖、黏附与侵袭的影响[J].中国肿瘤生物治疗杂志,2012,19(2):138-142.
[6]Bertelli AA,Baccalini R,Battaglia E,et al.Resveratrol inhibits TNF alpha-induced endothelial cell activation[J].Therapie,2001,56(5):613-616.
[7]刘焕义,赵煜,张小龙,等.白藜芦醇诱导肺腺癌A549细胞凋亡的机制[J].中国老年学杂志,2014,34(3):714-715.
[8]Garvin S,Ollinger K,Dabrosin C.Resveratrol induces apoptosis and inhibits angiogenesis in human breast cancer xenografts in vivo[J].Cancer Lett,2006,231(1):113-122.
[9]Saiko P,Szakmary A,Jaeger W,et al.Resveratrol and its analogs:defense against cancer,coronary disease and neurodegenerative maladies or just a fad?[J].Mutat Res,2008,658(1/2):68-94.
[10]Weng CJ,Yang YT,Ho CT,et al.Mechanisms of apoptotic effects induced by resveratrol,dibenzoylmethane,and their analogues on human lung carcinoma cells[J].J Agric Food Chem,2009,57(12):5 235-5 243.
[11]Zhou R,Fukui M,Choi HJ,et al.Induction of a reversible,non-cytotoxic S-phase delay by resveratrol:implications for a mechanism of lifespan prolongation and cancer protection[J].Br J Pharmacol,2009,158(2):462-474.
[12]Vanamala J,Reddivari L,Radhakrishnan S,et al.Resveratrol suppresses IGF-1 induced human colon cancer cell proliferation and elevates apoptosis via suppression of IGF-1R/Wnt and activation of p53 signaling pathways[J].BMC Cancer,2010,10:238.
[13]Hudson TS,Hartle DK,Hursting SD,et al.Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms[J].Cancer Res,2007,67(17):8 396-8 405.
[14]于柏艳,孙抒,杨万山,等.虎杖提取物对人肺癌A549细胞株抑制增殖和诱导凋亡作用的研究[J].中成药,2010,32(11):1 972-1 975.