海藻多糖可溶性粉增强猪免疫功能的临床应用研究
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摘要
为进一步探讨海藻多糖可溶性粉作为饲料添加剂在猪的规模化生产中大面积应用的临床效果及安全性,选取35～50日龄临床自然感染猪圆环病毒2型(PCV2)仔猪150头,随机分为3组,每组50头,分别设供试药物组(在饲料中添加海藻多糖可溶性粉200 mg/kg,连用7 d)、药物对照组(在饲料中添加黄芪多糖粉200 mg/kg,连用7 d)和阳性对照组(不处理)。于用药前1 d、用药后第7天和第14天每组随机选择10头试验猪测定血清中猪白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、干扰素-γ(INF-γ)、肿瘤坏死因子-α(TNF-α)等细胞因子和免疫球蛋白G(IgG)水平。结果表明,在临床自然感染猪圆环病毒2型(PCV2)的仔猪日粮里添加200 mg/kg海藻多糖可溶性粉能升高猪血清中IL-2、 IL-6、TNF-α、IFN-γ和IgG水平;试验期间,在仔猪日粮中添加200 mg/kg海藻多糖可溶性粉连用7 d无不良反应出现。由该试验结果可以得出,海藻多糖可溶性粉能够提高猪的免疫功能,临床使用是安全的。
In order to further evaluate the clinical efficacy and safety of seaweed polysaccharide soluble powder as a feed additive in large-scale application in swine production, a total of 150 naturally porcine circovirus type 2(PCV2) infected pigs 35 to 50 days of age were randomly divided into 3 groups with 50 pigs in each group. The tested drug group fed the diet with supplementation of 200 mg/kg seaweed polysaccharide soluble powder for 7 consecutive days. The drug control group whose diet was added with 200 mg/kg Astragalus polysaccharide powder for 7 consecutive days. The positive control group received no dietary addition treatment. Ten random serum samples from each group were collected on one day before treatment as well as the7 thand 14thday post treatment, respectively, and the serum levels of IL-2, IL-6, INF-γ, TNF-α and IgG were determined. The results showed the dietary supplementation of 200 mg/kg seaweed polysaccharide soluble powder elevated the serum levels of IL-2,IL-6, TNF-α, INF-γ and IgG in pigs with natural PCV2 infection; during the experimental period, no adverse reactions were observed in the swine fed with diet adding 200 mg/kg seaweed polysaccharide soluble powder for 7 consecutive days. The combined data suggest that seaweed polysaccharide soluble powder enhances the immune function of pig and is safe for clinical use.
In order to further evaluate the clinical efficacy and safety of seaweed polysaccharide soluble powder as a feed additive in large-scale application in swine production, a total of 150 naturally porcine circovirus type 2(PCV2) infected pigs 35 to 50 days of age were randomly divided into 3 groups with 50 pigs in each group. The tested drug group fed the diet with supplementation of 200 mg/kg seaweed polysaccharide soluble powder for 7 consecutive days. The drug control group whose diet was added with 200 mg/kg Astragalus polysaccharide powder for 7 consecutive days. The positive control group received no dietary addition treatment. Ten random serum samples from each group were collected on one day before treatment as well as the7 thand 14thday post treatment, respectively, and the serum levels of IL-2, IL-6, INF-γ, TNF-α and IgG were determined. The results showed the dietary supplementation of 200 mg/kg seaweed polysaccharide soluble powder elevated the serum levels of IL-2,IL-6, TNF-α, INF-γ and IgG in pigs with natural PCV2 infection; during the experimental period, no adverse reactions were observed in the swine fed with diet adding 200 mg/kg seaweed polysaccharide soluble powder for 7 consecutive days. The combined data suggest that seaweed polysaccharide soluble powder enhances the immune function of pig and is safe for clinical use.
引文
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[11]GUO Q,SHAO Q,XU W,et al.Immunomodulatory and anti-ibdv activities of the polysaccharide AEX from Coccomyxa gloeobotrydiformis[J].Marine Drugs,2017,15(2):36.
[12]YANG J,YANG F,YANG H,et al.Water-soluble polysaccharide isolated with alkali from the stem of Physalis alkekengi L.:Structural characterization and immunologic enhancement in DNA vaccine[J].Carbohydrate Polymers,2015,121:248-253.
[13]姚敬明,孟帆,王娟萍,等.黄芪多糖对猪瘟疫苗体液免疫和细胞免疫的影响[J].畜牧兽医科技信息,2016(9):15-18.
[14]刘娟妮,王雪青,魏丹,等.螺旋藻多糖的积累与抗肿瘤作用的研究[J].海洋通报,2009,28(6):41-45.
[15]许娇红,张熠,杨秀静,等.螺旋藻多糖增强免疫力功能试验研究[J].海峡药学,2018,30(1):31-33.
[2]JIANG M H,ZHU L,JIANG J G.Immunoregulatory actions of polysaccharides from Chinese herbal medicine[J].Expert Opin Ther Targets,2010,14(12):1367-1402.
[3]SHI Q,WANG A,LU Z,et al.Overview on the antiviral activities and mechanisms of marine polysaccharides from seaweeds[J].Carbohydrate Research,2017,453/454:1-9.
[4]周华杰,马兴亮,崔秀华,等.海藻多糖的生理功能及其在动物生产上的应用[J].饲料与畜牧,2013(3):23-26.
[5]LEONARD S G,SWEENEY T,BAHAR B,et al.Effect of maternal seaweed extract supplementation on suckling piglet growth,humoral immunity,selected microflora,and immune response after an ex vivo lipopolysaccharide challenge[J].Journal of Animal Science,2012,90(2):505-514.
[6]LEONARD S G,SWEENEY T,BAHAR B,et al.Effect of dietary seaweed extracts and fish oil supplementation in sows on performance,intestinal microflora,intestinal morphology,volatile fatty acid concentrations and immune status of weaned pigs[J].Br J Nutr,2011,105(4):549-560.
[7]林文君,郭蕾,王春梅.猪圆环病毒2型对猪的免疫抑制机理[J].畜牧兽医科技信息,2012(6):11-12.
[8]WANG Z,DONG B,FENG Z,et al.A study on immunomodulatory mechanism of polysaccharopeptide mediated by TLR4 signaling pathway[J].BMC Immunol,2015,16(1):34-42.
[9]马淑芳,王庆奎,戴伟,等.当归多糖对点带石斑鱼Toll样受体22信号通路相关基因表达的影响[J].大连海洋大学学报,2017,32(1):33-37.
[10]谭红连,杨剑,尹丹,等.马尾藻多糖对PCV2体外感染3D4/2细胞活性及炎症相关因子的影响[J].江苏农业科学,2017,45(23):166-168.
[11]GUO Q,SHAO Q,XU W,et al.Immunomodulatory and anti-ibdv activities of the polysaccharide AEX from Coccomyxa gloeobotrydiformis[J].Marine Drugs,2017,15(2):36.
[12]YANG J,YANG F,YANG H,et al.Water-soluble polysaccharide isolated with alkali from the stem of Physalis alkekengi L.:Structural characterization and immunologic enhancement in DNA vaccine[J].Carbohydrate Polymers,2015,121:248-253.
[13]姚敬明,孟帆,王娟萍,等.黄芪多糖对猪瘟疫苗体液免疫和细胞免疫的影响[J].畜牧兽医科技信息,2016(9):15-18.
[14]刘娟妮,王雪青,魏丹,等.螺旋藻多糖的积累与抗肿瘤作用的研究[J].海洋通报,2009,28(6):41-45.
[15]许娇红,张熠,杨秀静,等.螺旋藻多糖增强免疫力功能试验研究[J].海峡药学,2018,30(1):31-33.
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