中分化肺腺癌及癌旁组织中差异蛋白质组学分析
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摘要
目的分析人中分化肺腺癌及癌旁正常组织中的差异蛋白,寻找与肺腺癌发病相关的蛋白质。方法利用双向凝胶电泳法分离肺腺癌及癌旁正常组织中的蛋白质,通过图像扫描寻找差异2倍以上蛋白点,进而应用基质辅助激光电离飞行时间质谱得到肽质量指纹图谱,对比数据库确定差异蛋白。结果肺腺癌组织及癌旁正常组织平均蛋白点数分别为1 810±167、1 704±53。共筛选出18个差异蛋白点,其中13个差异蛋白点在肺腺癌组织中表达上升。结论双向凝胶电泳技术结合基质辅助激光电离飞行时间质谱可鉴定肺腺癌及癌旁正常组织的差异蛋白,为早期诊断肺癌、判断预后提供依据。
Objective To analyze the differential protein of human moderately differentiated lung adenocarcinoma and adjacent tissues,and find the protein related to the pathogenesis of lung adenocarcinoma. Methods The protein in lung adenocarcinoma and adjacent normal tissues were separated by using two dimensional gel electrophoresis( 2-DE),and the differential protein spots with a difference of more than two times were identified by image scanning. Then the peptide mass fingerprins of different protein were obtained by using matrix assisted laser ionization time of flight mass spectrometry,and the differential proteins were determined by comparing the databases. Results The average number of protein points in lung adenocarcinoma tissues and adjacent normal tissues were 1 810 ± 167 and 1 704 ± 53,respectively. A total of 18 differentially expressed protein spots were screened out,of which 13 differentially expressed protein spots increased in lung adenocarcinoma tissues. Conclusion 2-DE combined with matrix assisted laser ionization time of flight mass spectrometry can identify differential proteins in lung adenocarcinoma and adjacent normal tissues,and provide evidence for early diagnosis and prognosis of lung adenocarcinoma.
Objective To analyze the differential protein of human moderately differentiated lung adenocarcinoma and adjacent tissues,and find the protein related to the pathogenesis of lung adenocarcinoma. Methods The protein in lung adenocarcinoma and adjacent normal tissues were separated by using two dimensional gel electrophoresis( 2-DE),and the differential protein spots with a difference of more than two times were identified by image scanning. Then the peptide mass fingerprins of different protein were obtained by using matrix assisted laser ionization time of flight mass spectrometry,and the differential proteins were determined by comparing the databases. Results The average number of protein points in lung adenocarcinoma tissues and adjacent normal tissues were 1 810 ± 167 and 1 704 ± 53,respectively. A total of 18 differentially expressed protein spots were screened out,of which 13 differentially expressed protein spots increased in lung adenocarcinoma tissues. Conclusion 2-DE combined with matrix assisted laser ionization time of flight mass spectrometry can identify differential proteins in lung adenocarcinoma and adjacent normal tissues,and provide evidence for early diagnosis and prognosis of lung adenocarcinoma.
引文
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[15]LIU L K,WU H F,GUO X J,et al.Targeted efficacy of dihydroartemisinin for translationally controlled protein expression in a lung cancer model[J].Asian Pac J Cancer Prev,2014,15(6):2511-2515.
[2]POPPER H H.Progression and metastasis of lung cancer[J].Cancer Metastasis Rev,2016,35(1):75-91.
[3]JIN H,CHENG X,PEI Y,et al.Identification and verification of transgelin-2 as a potential biomarker of tumor derived lung cancer endothelial cells by comparative proteomics[J].J Proteomics,2016,136(7):77-88.
[4]GORG A,OBERMAIER C,WEISS W,et al.Two-dimensional electrophoresis of proteins in an immobilized p H 4-12 gradient[J].Electrophoresis,1998,19(8/9):1516-1519.
[5]PRINCIPEM M,CERUTI P,SHIH N Y,et al.Targeting of surface alpha-enolase inhibits the invasiveness of pancreatic cancer cells[J].Oncotarget,2015,6(13):11098-11113.
[6]SONG Y,LUO Q,LONG H,et al.Alpha-enolase as a potential cancer prognostic marker promotes cell growth,migration,and invasion in glioma[J].Mol Cancer,2014,21(13):65.
[7]HSIAO K C,SHIH N Y,FANG H L,et al.Surfaceα-enolase promotes extracellular matrix degradation and tumor metastasis and represents a new therapeutic target[J].PLo S One,2013,8(7):e69354.
[8]FU Q F,LIU Y,FAN Y,et al.Alpha-enolase promotes cell glycolysis,growth,migration,and invasion in non-small cell lung cancer through FAK-mediated PI3K/AKT pathway[J].J Hematol Oncol,2015,8(3):22-34.
[9]KARANTZA V.Keratins in health and cancer:more than mere epithelial cell markers[J].Oncogene,2011,30(2):127-138.
[10]韩彬彬,魏葆珺,冯莎娜,等.细胞角蛋白18在胃癌化疗疗效评价中临床意义[J].中华实用诊断与治疗杂志,2014,28(2):119-121.
[11]段楚骁,付圣灵,付向宁.联合检测在非小细胞肺癌中鉴别诊断的价值[J].临床肺科杂志,2015,20(1):115-118.
[12]黄冬云,许文景徐兴祥,等.细胞角蛋白在腺癌诊断中的作用[J].医学综述,2016,22(8):1499-1502.
[13]DIXIT U,LIU Z,PANDEY A K,et al.Fuse binding protein antagonizes the transcription activity of tumor suppressor protein p53[J].BMC Cancer,2014,14(12):925-937.
[14]JUNG J,KIM H Y,MAENG J,et al.Interaction of translationally controlled tumor protein with Apaf-1 is involved in the development of chemoresistance in He La cells[J].BMC Cancer,2014,14(3):165-178.
[15]LIU L K,WU H F,GUO X J,et al.Targeted efficacy of dihydroartemisinin for translationally controlled protein expression in a lung cancer model[J].Asian Pac J Cancer Prev,2014,15(6):2511-2515.