重力场流分离系统用于聚苯乙烯颗粒的分离条件优化
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摘要
重力场流分离是最简单的场流分离(gravitational flow-field fractionation,GrFFF)技术,常用于分离粒径几微米到几十微米的颗粒及生物样品。利用自组装加工的重力场流分离仪器分离3种不同粒径(3、6、20μm)的聚苯乙烯(PS)颗粒。自制了一种混合表面活性剂,并与商品化的表面活性剂FL-70进行了比较。通过均匀设计优化流速、混合表面活性剂中聚乙二醇辛基苯基醚(Triton X-100)的质量分数、载液黏度、停流时间等分离条件,以分离度(Rs)和保留比(R)为评价指标,发现FL-70的分离效能略优于自制的混合表面活性剂,可实现3种PS颗粒的完全分离(Rs1为1.771,Rs2为2.074)。结果表明该系统具有良好的分离性能。
Gravitational flow-field fractionation(GrFFF)is the simplest and cheapest fieldflow fractionation technique. GrFFF utilizes earth's gravity as external field,providing a sizebased separation. It can be applied to the separation and characterization of various micrometer-sized particles and biological samples. A GrFFF device was assembled,and three kinds of polystyrene(PS)particles with different sizes(20,6 and 3 μm)were used as the model particles. A homemade mixed surfactant was used and was compared with the surfactant FL-70. The separation conditions of GrFFF were optimized with uniform design,including flow rate,mass percentage of polyethylene glycol octylphenol ether(Triton X-100)in mixed surfactant,viscosity of carrier liquid and stop-flow time. The resolution(Rs)and retention ratio(R)were utilized as the evaluation indexes. The results suggest that the separation performance of FL-70 was slightly better than that of the mixed surfactant. Using FL-70 as the surfactant,particles of 20,6 and 3 μm were completely separated(with resolutions of 1. 771 and 2. 074). The results indicate that this system has good separation performance.
Gravitational flow-field fractionation(GrFFF)is the simplest and cheapest fieldflow fractionation technique. GrFFF utilizes earth's gravity as external field,providing a sizebased separation. It can be applied to the separation and characterization of various micrometer-sized particles and biological samples. A GrFFF device was assembled,and three kinds of polystyrene(PS)particles with different sizes(20,6 and 3 μm)were used as the model particles. A homemade mixed surfactant was used and was compared with the surfactant FL-70. The separation conditions of GrFFF were optimized with uniform design,including flow rate,mass percentage of polyethylene glycol octylphenol ether(Triton X-100)in mixed surfactant,viscosity of carrier liquid and stop-flow time. The resolution(Rs)and retention ratio(R)were utilized as the evaluation indexes. The results suggest that the separation performance of FL-70 was slightly better than that of the mixed surfactant. Using FL-70 as the surfactant,particles of 20,6 and 3 μm were completely separated(with resolutions of 1. 771 and 2. 074). The results indicate that this system has good separation performance.
引文
[1]Giddings J C.Sep Sci,1966,1:123
[2]Schachermeyer S,Ashby J,Zhong W W.Anal Bioanal Chem,2012,404:1151
[3]Quan C,Liu P P,Jin J S.Chemistry,2013,76(1):52全灿,刘攀攀,金君素.化学通报,2013,76(1):52
[4]Park M R,Kang D K,Chmelik J,et al.J Chromatogr A,2008,1209:206
[5]Plockva J,Chmelik J.J Chromatogr A,2001,918:361
[6]Plockva J,Chmelik J.J Chromatogr A,2006,1118:253
[7]Lee S,Kang D Y,Park M,et al.Anal Chem,2011,83:3343
[8]Reschiglian P,Melucci D,Torsi G.J Chromatogr A,1996,740:245
[9]Jiang D L,Shao L Y.Journal of Jilin Normal University:Natural Science Edition,2009,30(1):78蒋东霖,邵丽颖.吉林师范大学学报:自然科学版,2009,30(1):78
[10]Plockva J,Matulik F,Chmelik J.J Chromatogr A,2002,955:95
[11]Shao L Y,Jiang D L.Journal of Jilin Normal University:Natural Science Edition,2011,32(1):91邵丽颖,蒋东霖.吉林师范大学学报:自然科学版,2011,32(1):91
[12]Lattuade D,Roda B,Pignatari C,et al.Anal Bioanal Chem,2013,405:977
[13]Roda B,Casolari S,Reschiglian P,et al.Anal Bioanal Chem,2009,394:953
[14]Casolari S,Roda B,Mirasoli M,et al.Analyst,2013,138:211
[15]Guo S,Zhu C Q,Gaoyang Y Y,et al.Chinese Journal of Chromatography,2016,34(2):146郭爽,朱尘琪,高杨亚雅,等.色谱,2016,34(2):146
[16]Zhang X J.[PhD Dissertation].Changchun:Jilin University,2007张学军.[博士学位论文].长春:吉林大学,2007
[17]Woo I S,Jung E C,Lee S.Talanta,2015,132:945
[18]Cen G Q.[MS Dissertation].Haikou:Hainan University,2012岑桂秋.[硕士学位论文].海口:海南大学,2012
[19]Shao L Y,Jiang D L,LüY J.Journal of Yanbian University:Natural Science,2008,34(4):258邵丽颖,蒋东霖,吕英军.延边大学学报:自然科学版,2008,34(4):258.
[2]Schachermeyer S,Ashby J,Zhong W W.Anal Bioanal Chem,2012,404:1151
[3]Quan C,Liu P P,Jin J S.Chemistry,2013,76(1):52全灿,刘攀攀,金君素.化学通报,2013,76(1):52
[4]Park M R,Kang D K,Chmelik J,et al.J Chromatogr A,2008,1209:206
[5]Plockva J,Chmelik J.J Chromatogr A,2001,918:361
[6]Plockva J,Chmelik J.J Chromatogr A,2006,1118:253
[7]Lee S,Kang D Y,Park M,et al.Anal Chem,2011,83:3343
[8]Reschiglian P,Melucci D,Torsi G.J Chromatogr A,1996,740:245
[9]Jiang D L,Shao L Y.Journal of Jilin Normal University:Natural Science Edition,2009,30(1):78蒋东霖,邵丽颖.吉林师范大学学报:自然科学版,2009,30(1):78
[10]Plockva J,Matulik F,Chmelik J.J Chromatogr A,2002,955:95
[11]Shao L Y,Jiang D L.Journal of Jilin Normal University:Natural Science Edition,2011,32(1):91邵丽颖,蒋东霖.吉林师范大学学报:自然科学版,2011,32(1):91
[12]Lattuade D,Roda B,Pignatari C,et al.Anal Bioanal Chem,2013,405:977
[13]Roda B,Casolari S,Reschiglian P,et al.Anal Bioanal Chem,2009,394:953
[14]Casolari S,Roda B,Mirasoli M,et al.Analyst,2013,138:211
[15]Guo S,Zhu C Q,Gaoyang Y Y,et al.Chinese Journal of Chromatography,2016,34(2):146郭爽,朱尘琪,高杨亚雅,等.色谱,2016,34(2):146
[16]Zhang X J.[PhD Dissertation].Changchun:Jilin University,2007张学军.[博士学位论文].长春:吉林大学,2007
[17]Woo I S,Jung E C,Lee S.Talanta,2015,132:945
[18]Cen G Q.[MS Dissertation].Haikou:Hainan University,2012岑桂秋.[硕士学位论文].海口:海南大学,2012
[19]Shao L Y,Jiang D L,LüY J.Journal of Yanbian University:Natural Science,2008,34(4):258邵丽颖,蒋东霖,吕英军.延边大学学报:自然科学版,2008,34(4):258.