疏肝利胆颗粒对胆总管结石术后胆汁IL-6、IL-8及血清CRP的影响
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摘要
目的研究疏肝利胆颗粒对胆总管结石术后胆汁白介素(interleukin,IL)-6、IL-8及血清C型反应性蛋白(C-reactive protein,CRP)的影响,探讨疏肝利胆颗粒对术后胆汁炎性因子的改善机制。方法将72例胆总管结石术后患者随机分为对照组与疏肝利胆颗粒治疗组(简称干预组)。干预组在常规治疗的基础上给予疏肝利胆颗粒治疗,每日1剂,共28 d。于术中(第1天)、第7天、第14天及第28天,分别取2组胆汁上清液及血清进行酶联免疫吸附测定(enzyme-linked immunosorbent assay,EILSA)检测IL-6、IL-8表达水平及检验科检测CRP血清表达水平。结果干预组胆汁中IL-6及IL-8表达水平在第7天、第14天及第28天血清表达水平较对照组显著降低(P<0.05或P<0.01)。干预组CRP表达较对照组表达亦显著降低(P<0.05或P<0.01)。结论疏肝利胆颗粒可以显著降低胆汁中IL-6及IL-8的表达水平,减少血清中CRP的表达水平,从而减轻胆汁的炎症反应,减少胆汁成石的炎性因素。
Objective To study the effects and mechanisms of on bile IL-6 and IL-8 and serum CRP levels after choledocholithotomy.Methods Seventy-two patients underwent choledocholithotomy were randomly divided into the control group and intervening group.The intervening group patients were given Shugan Lidan granules once a day for 28 days. The bile supernatant was obtained on the day 1, 7, 14 and 28. The levels of IL-6 and IL-8 were measured by enzyme-linked immunosorbent assay(EILSA). C-reactive protein(CRP) levels were detected by clinical lab. Results The levels of IL-6, IL-8 and CRP with Shugan Lidan granules treatment were downregulated comparing with control group on the day 7, 14 and 28(P<0.05 or P<0.01). Conclusion Shugan Lidan granules could alleviate inflammation and reduce risk factors of gallstone by downregulating the levels of IL-6, IL-8 and CRP.
Objective To study the effects and mechanisms of on bile IL-6 and IL-8 and serum CRP levels after choledocholithotomy.Methods Seventy-two patients underwent choledocholithotomy were randomly divided into the control group and intervening group.The intervening group patients were given Shugan Lidan granules once a day for 28 days. The bile supernatant was obtained on the day 1, 7, 14 and 28. The levels of IL-6 and IL-8 were measured by enzyme-linked immunosorbent assay(EILSA). C-reactive protein(CRP) levels were detected by clinical lab. Results The levels of IL-6, IL-8 and CRP with Shugan Lidan granules treatment were downregulated comparing with control group on the day 7, 14 and 28(P<0.05 or P<0.01). Conclusion Shugan Lidan granules could alleviate inflammation and reduce risk factors of gallstone by downregulating the levels of IL-6, IL-8 and CRP.
引文
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[2] Cai J S,Qiang S,Bao-Bing Y. Advances of recurrent risk factors and management of choledocholithiasis[J]. Scand J Gastroenterol,2017,52(1):34-43.
[3] Kaufman H S,Magnuson T H,Lillemoe K D,et al. The role of bacteria in gallbladder and common duct stone formation[J]. Ann Surg,1989,209(5):584-591.
[4] Chopra K B,Peters R A,O'Toole P A,et al. Randomised study of endoscopic biliary endoprosthesis versus duct clearance for bileduct stones in high-risk patients[J]. Lancet,1996,348(9030):791-793.
[5] Caddy G R,Kirby J,Kirk S J,et al. Natural history of asymptomatic bile duct stones at time of cholecystectomy[J]. Ulster Med J,2005,74(2):108-112.
[6]张朋飞,宿广峰,韩娜娜,等.疏肝利胆颗粒对胆管结石术后胆汁成分的影响[J].山东中医药大学学报,2017,41(5):429-431.
[7] Aspinen S,Kinnunen M,Harju J,et al. Inflammatory response to surgical trauma in patients with minilaparotomy cholecystectomy versus laparoscopic cholecystectomy:a randomised multicentre study[J]. Scand J Gastroenterol,2016,51(6):739-744.
[8] Bastard J P,Jardel C,Delattre J,et al. Evidence for a link between adipose tissue interleukin-6 content and serum C-reactive protein concentrations in obese subjects[J]. Circulation,1999,99(16):2221-2222.
[9] Dusaban S S,Chun J,Rosen H,et al. Sphingosine 1-phosphate receptor 3and Rho A signaling mediate inflammatory gene expression in astrocytes[J].JNeuroinflammation,2017,14(1):111-120.
[10] Woo J H,Yang Y I,Ahn J H,et al. Interleukin 6 secretion from alternatively activated macrophages promotes the migration of endometriotic epithelial cells[J]. Biol Reprod,2017,97(5):660-670.
[11] Saito S,Suzuki K,Yoshimoto K,et al. A new bioassay for measuring the strength of IL-6/STAT3 signal inhibition by tocilizumab in patients with rheumatoid arthritis[J]. Arthritis Res Ther,2017,19(1):231-238.
[12] Chen L,Liu X,Pan Z,et al. The role of IL-8/CXCR2 signaling in microcystin-LR triggered endothelial cell activation and increased vascular permeability[J]. Chemosphere,2017,194:43-48.
[13]张利霞,沈云志,邱全兴,等.胆汁中IL-6、TNF-α、IL-8检测对急性胆管炎诊断和严重程度的判断价值[J].国际消化病杂志,2008,28(4):348-350.
[14] Bishayi B,Adhikary R,Sultana S,et al. Altered expression of CXCR1(IL-8R)in macrophages utilizing cell surface TNFR1 and IL-1 receptor during Staphylococcus aureus infection[J]. Microb Pathog,2017,113:460-471.
[15] Candel-MartíM E,Flichy-Fernández A J,Alegre-Domingo T,et al. Interleukins IL-6,IL-8,IL-10,IL-12 and periimplant disease. An update[J].Med OralPatolOralCir Bucal,2011,16(4):e518-521.
[16] Chew K S. What's new in Emergencies Trauma and Shock? C-reactive protein as a potential clinical biomarker for influenza infection:More questions than answers[J]. J Emerg Trauma Shock,2012,5(2):115-117.
[17] Thompson D,Pepys M B,Wood S P. The physiological structure of human C-reactive protein and its complex with phosphocholine[J]. Structure,1999,7(2):169-177.
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