饲料中添加谷胱甘肽对花鲈幼鱼生长性能、血清生化指标和抗氧化能力的影响
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摘要
本试验旨在研究饲料中添加谷胱甘肽对花鲈幼鱼生长性能、血清生化指标和抗氧化能力的影响。选用初始体重为(12.56±0.12) g的花鲈600尾,随机分为6个组,每组4个重复,每个重复25尾鱼。对照组饲喂基础饲料,试验组饲喂在基础饲料中分别添加100、300、400、500和700 mg/kg谷胱甘肽的试验饲料。试验期56 d。结果表明:1)各组间生长性能和常规营养成分指标无显著差异(P>0.05)。2)与对照组相比,100 mg/kg组血清葡萄糖和尿素氮含量以及700 mg/kg组血清葡萄糖含量及谷草转氨酶、谷丙转氨酶活性均显著升高(P <0.05)。3)与对照组相比,500和700 mg/kg组血清总抗氧化能力、谷胱甘肽含量和谷胱甘肽过氧化物酶活性以及各试验组血清谷胱甘肽巯基转移酶活性均显著升高(P <0. 05)。4)与对照组相比,300和400 mg/kg组肝脏超氧化物歧化酶活性以及100、400、500和700 mg/kg组肝脏谷胱甘肽过氧化物酶活性均显著升高(P<0.05),100和700 mg/kg组肝脏谷胱甘肽巯基转移酶活性显著降低(P<0.05)。5)与对照组相比,300 mg/kg组肝脏白细胞介素-6含量显著升高(P<0.05)。由此可见,饲料中添加谷胱甘肽可提高花鲈血清和肝脏一些抗氧化指标,但对于部分抗氧化指标和肝脏炎症因子指标却呈负面影响。因此,在本试验基础饲料条件下,花鲈饲料中应当慎重添加谷胱甘肽。
This experiment was conducted to evaluate the effects of dietary glutathione on growth performance,serum biochemical indices and antioxidant capacity of juvenile Lateolabrax japonicus. A total of 600 Lateolabrax japonicus with initial body weight of( 12.56±0.12) g were randomly divided into 6 groups with 4 replicates per group and 25 fish per replicate. Fish in the control group were fed a basal diet,and the others in the experimental groups were fed the basal diet supplemented with 100,300,400,500 and 700 mg/kg glutathione,respectively. The trial lasted for 56 days. The results showed as follows: 1) there were no significant differences in growth performance and routine nutritional component indices among all groups( P > 0.05). 2)Compared with the control group,the contents of glucose and urea nitrogen in serum in 100 mg/kg group and the glucose content and aspartate aminotransferase,alanine aminotransferase activities in serum in 700 mg/kg group were significantly increased( P < 0. 05). 3) Compared with the control group,the serum total antioxidant,glutathione content and glutathione perxidase activity in 500 and 700 mg/kg groups and the serum glutathione transferase activity in all experimental groups were significantly increased( P<0.05). 4) Compared with the control group,the liver superoxide dismutase activity in 300 and 400 mg/kg groups and the liver glutathione perxidase activity in 100,400,500 and 700 mg/kg groups were significantly increased( P < 0.05),and the liver glutathione transferase activity in 100 and 700 mg/kg groups was significantly decreased( P < 0.05).5) Compared with the control group,the liver interleukin-6 content in 300 mg/kg group was significantly increased( P<0.05). In conclusion,dietary glutathoine can improve the antioxidant indices in serum and liver of Lateolabrax japonicus,but it has a negative impact on some antioxidant indexes and liver inflammatory factors.Therefore,under the condition of the basal diet in this experiment,glutathione should be carefully added into the diet of Lateolabrax japonicus.[Chinese Journal of Animal Nutrition,2019,31(7):3207-3217]
This experiment was conducted to evaluate the effects of dietary glutathione on growth performance,serum biochemical indices and antioxidant capacity of juvenile Lateolabrax japonicus. A total of 600 Lateolabrax japonicus with initial body weight of( 12.56±0.12) g were randomly divided into 6 groups with 4 replicates per group and 25 fish per replicate. Fish in the control group were fed a basal diet,and the others in the experimental groups were fed the basal diet supplemented with 100,300,400,500 and 700 mg/kg glutathione,respectively. The trial lasted for 56 days. The results showed as follows: 1) there were no significant differences in growth performance and routine nutritional component indices among all groups( P > 0.05). 2)Compared with the control group,the contents of glucose and urea nitrogen in serum in 100 mg/kg group and the glucose content and aspartate aminotransferase,alanine aminotransferase activities in serum in 700 mg/kg group were significantly increased( P < 0. 05). 3) Compared with the control group,the serum total antioxidant,glutathione content and glutathione perxidase activity in 500 and 700 mg/kg groups and the serum glutathione transferase activity in all experimental groups were significantly increased( P<0.05). 4) Compared with the control group,the liver superoxide dismutase activity in 300 and 400 mg/kg groups and the liver glutathione perxidase activity in 100,400,500 and 700 mg/kg groups were significantly increased( P < 0.05),and the liver glutathione transferase activity in 100 and 700 mg/kg groups was significantly decreased( P < 0.05).5) Compared with the control group,the liver interleukin-6 content in 300 mg/kg group was significantly increased( P<0.05). In conclusion,dietary glutathoine can improve the antioxidant indices in serum and liver of Lateolabrax japonicus,but it has a negative impact on some antioxidant indexes and liver inflammatory factors.Therefore,under the condition of the basal diet in this experiment,glutathione should be carefully added into the diet of Lateolabrax japonicus.[Chinese Journal of Animal Nutrition,2019,31(7):3207-3217]
引文
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[2]贾贞,王丹,游松.谷胱甘肽的研究进展[J].沈阳药科大学学报,2009,26(3):238-242.
[3]王大慧,卫功元.谷胱甘肽的应用前景及生产研究现状[J].化学与生物工程,2004,21(3):10-12.
[4]胡圣尧,聂志妍,袁勤生.还原型谷胱甘肽的研究进展[J].食品与药品,2009,11(1):69-71.
[5] TREVISANI F,TAMMR,BERNARDI M,et al.Hepatic failure occurring with T61 ingestion in an Attempted Suicide:early recovery with the use of intravenous infusion of reduced glutathione[J]. Digestive Diseases and Sciences,1993,38(4):752-756.
[6]杨斌.谷胱甘肽在临床上的新用途[J].安徽医药,2000,4(4):29.
[7]陈金永,李朕杰.谷胱甘肽—一种新型饲用添加剂[J].中国动物保健,2005(5):40-41.
[8]刘超,袁建国,李峰.谷胱甘肽国内外研究进展[J].山东食品发酵,2010(4):7-10.
[9]傅伟龙,江青艳,刘平祥,等.谷胱甘肽的生物学功能及其应用[C]//全国反刍动物营养生理生化第三次学术会议论文摘要汇编.长春:中国畜牧兽医学会,2004:33-37.
[10]赵红霞,曹俊明,朱选,等.日粮添加谷胱甘肽对草鱼生长性能血清生化指标和体组成的影响[J].动物营养学报,2008,20(5):540-546.
[11] YUAN X,ZHOU Y,LIANG X F,et al.Effect of Dietary glutathione supplementation on the biological value of rapeseed meal to juvenile grass carp,Ctenopharyngodon idellus[J]. Aquaculture Nutrition,2015,21(1):73-84.
[12]梁春梅.还原型谷胱甘肽对奥尼罗非鱼(Oreochromis Niloticus×O.Aureus)幼鱼生长、免疫功能的影响及其机理研究[D].硕士学位论文.广州:华南农业大学,2006.
[13]王芳倩.饲料中还原型谷胱甘肽、α-硫辛酸及二者分别与硒的交互作用对牙鲆(Paralichthys olivaceus)生长和抗氧化的影响[D].硕士学位论文.青岛:中国海洋大学,2010.
[14]刘晓华.谷胱甘肽对凡纳滨对虾抗氧化防御的调控机理[D].博士学位论文.武汉:华中农业大学,2010.
[15]周婷婷.谷胱甘肽对吉富罗非鱼生长性能和抗氧化功能的影响[D].硕士学位论文.武汉:华中农业大学,2012.
[16]周婷婷,曹俊明,黄燕华,等.饲料中添加谷胱甘肽对吉富罗非鱼生长、组织生化指标和非特异性免疫相关酶的影响[J].水产学报,2013,37(5):742-750.
[17]刘晓华,曹俊明,朱选,等.谷胱甘肽对凡纳滨对虾生长、存活和饵料系数的影响[J].饲料工业,2007,28(8):21-23.
[18]任胜杰,郑曙明,宋艳,等.谷胱甘肽对异育银鲫生长及辛硫磷解毒的影响[J].西南农业学报,2016,29(12):3009-3014.
[19]陈齐勇.饲料中α-硫辛酸、谷胱甘肽和硒对皱纹盘鲍(Haliotis discus hannai Ino)生长和抗氧化反应的影响[D].硕士学位论文.青岛:中国海洋大学,2010.
[20] GATLIN D M,BAI S C. Effects of dietary lipid and reduced glutathione on composition and storage quality of channel catfish,Ictalurus punctatus(Rafinesque)[J].Aquaculture Research,1993,24(4):457-463.
[21]王芳倩,张文兵,麦康森,等.饲料中添加还原型谷胱甘肽对牙鲆生长和抗氧化能力的影响[J].中国海洋大学学报,2011,41(4):51-56.
[22]姚晨阳,刘敬,张启美,等.谷胱甘肽在水产养殖中的应用研究进展[J].山东科学,2016,19(1):105-109.
[23]苟庚午.四种饲料添加剂在吉富罗非鱼饲料中的应用效果[D].硕士学位论文.上海:上海海洋大学,2016.
[24]洪瑞川,李思光,余扬帆.万安玻璃红鲤的肌肉营养成分分析[J].水生生物学报,1997,21(2):109-113.
[25]周艳玲,孙育平,曹俊明,等.饲料中添加谷胱甘肽对黄颡鱼幼鱼生长性能、体成分、血清生化指标和抗氨氮应激能力的影响[J].动物营养学报,2017,29(11):4189-4197.
[26]韩娜娜,史成银.血液指标在鱼类学研究中的应用[J].安徽农业科学,2010,38(33):18877-18878,18880.
[27]刘红柏,宿斌,王荻.三种中草药方剂对鲫部分生化指标的影响[J].水产学杂志,2012,25(4):24-28.
[28]林谦,戴求仲,宾石玉,等.益生菌与酶制剂对黄羽肉鸡血液生化指标和免疫性能影响的协同效应研究[J].饲料工业,2012,33(14):31-36.
[29]田允波,周佳容,李琦华,等.白术多糖对仔猪生长性能和血清生化参数的影响[J].中国畜牧杂志,2009,45(9):45-48.
[30]许先查,王建红,刁其玉,等.代乳粉的饲喂水平对犊牛消化代谢及血清生化指标的影响[J].动物营养学报,2011,23(4):654-661.
[31]陈玉春,顾雪飞,刘敏.5种中草药对鲤血清谷丙转氨酶、谷草转氨酶及红细胞过氧化氢酶活性的影响[J].淡水渔业,2007,37(5):11-13.
[32] ZHANG H,MU Z,XU L M,et al.Dietary lipid level induced antioxidant response in manchurian trout,Brachymystax lenok(Pallas)Larvae[J]. Lipids,2009,44(7):643-654.
[33]韩宇哲,姜志强,任同军,等.氧化鱼油与棕榈油对花鲈肝脏抗氧化酶及组织结构的影响[J].中国水产科学,2010,17(4):798-806.
[34] FU S J,ZHENG L Q,LI X M,et al.The behavioural,digestive and metabolic characteristics of fishes with different foraging strategies[J].Journal of Experimental Biology,2009,212(14):2296-2302.
[35] HERNNDEZ MD,MENDIOLA P,DE COSTA J,et al. Effects of intense exercise training on rainbowtrout growth,body composition and metabolic responses[J]. Journal of Physiology and Biochemistry,2002,58(1):1-7.
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