甘蓝型油菜茎秆强度性状的主基因+多基因遗传分析
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摘要
为辅助抗倒伏育种,了解甘蓝型油菜茎秆强度的遗传调控,利用植物数量性状主基因+多基因混合遗传分离分析方法,对M417×Brongoro(MB)组合和浙油18×Brongoro(ZB)组合六个世代(P_1、P_2、F_1、B_(1∶2)、B_(2∶2)和F_(2∶3))的茎秆强度性状进行遗传分析。结果显示:甘蓝型油菜茎秆强度性状的最佳遗传模型为MX2-ADI-ADI,即2对加性-显性-上位性主基因+加性-显性-上位性多基因模型,表明该性状受2对主基因和微效多基因共同控制,以主基因遗传为主,MB组合和ZB组合的平均主基因遗传率分别为19.46%和69.93%。2对主基因的加性效应和显性效应在MB组合中作用方向相反,而在ZB组合中作用方向相同,同时还存在多种上位性效应。两个组合中环境变异占表型变异的54.68%和13.23%,说明环境对茎秆强度性状具有较大影响。
To understand the genetic control of stem strength in Brassica napus,the ‘major gene and polygene mixed genetic model'was used to analyze the stem strength trait of 6 generations( P_1,P_2,F_1,B_(1∶ 2),B_(2∶ 2) and F_(2∶ 3))in both MB and ZB hybrid combinations. Results indicated that stem strength genetic was fitted in MX2-ADI-ADI model,i. e.,‘2 pairs of additive-dominant-epistatic major gene plus additive-dominant-epistatic polygene'model. Stem strength was mainly controlled by 2 major genes and the average heritability of major gene were 19. 46% and 69. 93% in MB and ZB combinations respectively. The additive effects and dominant effects of the 2 major genes were in opposite direction in MB combination,but in same direction in ZB combination. Moreover,multiple epistatic effects influenced the 2 major genes. Proportion of environmental variance to phenotypic variance in these 2 combinations were 54. 68% and 13. 23% respectively,meaning that environment factors had strong impact on stem strength in rapeseed.
To understand the genetic control of stem strength in Brassica napus,the ‘major gene and polygene mixed genetic model'was used to analyze the stem strength trait of 6 generations( P_1,P_2,F_1,B_(1∶ 2),B_(2∶ 2) and F_(2∶ 3))in both MB and ZB hybrid combinations. Results indicated that stem strength genetic was fitted in MX2-ADI-ADI model,i. e.,‘2 pairs of additive-dominant-epistatic major gene plus additive-dominant-epistatic polygene'model. Stem strength was mainly controlled by 2 major genes and the average heritability of major gene were 19. 46% and 69. 93% in MB and ZB combinations respectively. The additive effects and dominant effects of the 2 major genes were in opposite direction in MB combination,but in same direction in ZB combination. Moreover,multiple epistatic effects influenced the 2 major genes. Proportion of environmental variance to phenotypic variance in these 2 combinations were 54. 68% and 13. 23% respectively,meaning that environment factors had strong impact on stem strength in rapeseed.
引文
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[2]Christou P,Savin R,Costa-Pierce B A,et al.Sustainable food production[A].Berry P M.Lodging Resistance in Cereals[C].New York:Springer New York,2013.1 096
[3]韩慧杰.甘蓝型油菜耐菌核病抗倒伏新种质的创建[D].武汉:华中农业大学,2009.9-12.
[4]Wei L J,Jian H J,Lu K,et al.Genome-wide association analysis and differential expression analysis of resistance to Sclerotinia stem rot in Brassica napus[J].Plant Biotechnol J,2016,14(6):1 368-1 380.
[5]Shah A N,Tanveer M,Rehman A U,et al.Lodging stress in cereal-effects and management:an overview[J].Environ Sci Pollut Res Int,2017,24(6):5 222-5 237.
[6]Islam M S,Peng S B,Visperas R M,et al.Lodging-related morphological traits of hybrid rice in a tropical irrigated ecosystem[J].Field Crop Res,2007,101(2):240-248.
[7]官邑.油菜抗倒伏性及其影响因素[J].作物研究,2014,28(2):216-220.
[8]Wei L J,Jian H J,Lu K,et al.Genetic and transcriptomic analyses of lignin-and lodging-related traits in Brassica napus[J].Theor Appl Genet,2017,130(9):1 961-1 973.
[9]姜维梅,张冬青,徐春霄.油菜茎的解剖结构和倒伏关系的研究[J].浙江大学学报(农业与生命科学版),2001,27(4):87-90.
[10]师恭曜.甘蓝型油菜茎秆抗倒伏性构成因素的鉴定与评价[D].郑州:郑州大学,2010,15-33;43-55.
[11]李尧臣,顾慧,戚存扣.抗倒伏甘蓝型油菜(Brassica napus L.)根和茎解剖学结构分析[J].江苏农业学报,2011,27(1):36-44.
[12]吴莲蓉.油菜茎秆生化成分和倒伏相关性研究[D].武汉:华中农业大学,2015,8-35.
[13]顾慧,戚存扣.甘蓝型油菜(Brassica napus L.)抗倒伏性状的主基因+多基因遗传分析[J].作物学报,2008,34(3):376-381
[14]张文华.甘蓝型油菜抗倒伏相关性状的遗传分析和QTL定位[D].武汉:华中农业大学,2010,17-21.
[15]顾慧,戚存扣.甘蓝型油菜(Brassica napus L.)抗倒伏性状的QTL分析[J].江苏农业学报,2009,25(3):484-489.
[16]彭旭辉.甘蓝型油菜抗倒伏指标的选取及其QTL定位[D].重庆:西南大学,2012,23-39.
[17]李扬,顾慧,戚存扣.甘蓝型油菜(Brassica napus L.)RIL群体抗倒伏及其相关性状的QTL分析[J].中国油料作物学报,2014,36(1):10-17.
[18]Kashiwagi T,Ishimaru K.Identification and Functional Analysis of a Locus for Improvement of Lodging Resistance in Rice[J].Plant physiology,2004,134(2):676.
[19]盖钧镒.植物数量性状遗传体系的分离分析方法研究[J].遗传,2005,27(1):130-136.
[20]丰光,刘志芳,李妍妍,等.玉米茎秆耐穿刺强度的倒伏遗传研究[J].作物学报,2009,35(11):2 133-2 138.
[21]李洪戈,余坤江,郭婷婷,等.甘蓝型油菜无花瓣性状的主基因+多基因遗传分析[J].江苏农业学报,2014,30(2):253-258.
[22]王利民,张建平,党照,等.胡麻温敏雄性不育产量相关性状主基因+多基因混合遗传分析[J].中国油料作物学报,2016,38(2):186-194.
[23]曹锡文,刘兵,章元明.植物数量性状分离分析Windows软件包SEA的研制[J].南京农业大学学报,2013,36(6):1-6.
[24]Ihaka R,Gentleman R.R:A language for data analysis and graphics[J].J Comput Graph Stat,1996,5(3):299-314.
[25]刘小刚,马飞前,王红武,等.玉米茎秆穿刺强度遗传研究[J].作物杂志,2014(4):27-31.
[26]胡丹.甜荞抗倒伏相关性状的遗传分析及木质素合成特征[D].重庆:西南大学,2016:13-38.