不同氮素基追比例对枸杞甜菜碱及相关酶含量的影响
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摘要
以5年生枸杞品种"宁杞7号"为试材,运用完全随机区组设计方法,设计1∶1、2∶1∶1、3∶1、3∶2∶1共4个不同氮肥追施比例水平,研究宁夏枸杞施氮不同追肥比例与甜菜碱含量及其代谢关键酶之间的关系。结果表明:氮肥不同追施比例对枸杞光合有明显的影响,前期追氮较少时光合速率相应较低,反之较高。整个生育期追肥比例以2∶1∶1时,其平均光合速率相对较高,说明枸杞生育期间氮肥相对均衡施用对其光合速率有益;氮肥的适度后移会相应增加枸杞单加氧酶含量,前期或后期追氮太多,均不利于枸杞单加氧酶累积;生育期内适度均匀施氮,利于枸杞甜菜碱醛脱氢酶(BADH)积累;甜菜碱含量与不同时期施氮量有直接关系,不同时期施氮较高时甜菜碱含量较高,667 m~2枸杞产量最高为276 kg(干质量)时氮肥追肥比例为2∶1∶1。
To study the relationship between the ratio of nitrogen fertilizer applied and the content of betaine and its metabolic key enzymes in Lycium barbarum L.four different top dressing nitrogen fertilizer applicationratio levels,namely 1∶1,2∶1∶1,3∶1 and 3∶2∶1 were adoptedby taking the five-year-old 'Ningqi No.7' as the test material.The results showed that the ratio of top dressing nitrogen fertilizer application had a significant effect on the photosynthetic rate of Lycium barbarum L..The average photosynthetic rate was relatively high at 2∶1∶1 during the whole growth period,which indicated that the relatively balanced application of nitrogen fertilizer was beneficial to the photosynthetic rate.The content of monooxygenase would be increased in Lycium barbarum L.,while nitrogen fertilizer applicationdelayed,otherwise,too much nitrogen in the early or late growing stages.Application nitrogen evenly contributed to accumulation of betaine-aldehyde dehydrogenase(BADH) during growing season.The betaine content was directly related to the amount of nitrogen applied at different times,and the content was higher along with the nitrogen applied higher.The yield of Lycium barbarum L.was the highest at 2∶1∶1 with the ratio of nitrogen fertilizer applied,which was 276 kg per 667 m~2(dry weight).
To study the relationship between the ratio of nitrogen fertilizer applied and the content of betaine and its metabolic key enzymes in Lycium barbarum L.four different top dressing nitrogen fertilizer applicationratio levels,namely 1∶1,2∶1∶1,3∶1 and 3∶2∶1 were adoptedby taking the five-year-old 'Ningqi No.7' as the test material.The results showed that the ratio of top dressing nitrogen fertilizer application had a significant effect on the photosynthetic rate of Lycium barbarum L..The average photosynthetic rate was relatively high at 2∶1∶1 during the whole growth period,which indicated that the relatively balanced application of nitrogen fertilizer was beneficial to the photosynthetic rate.The content of monooxygenase would be increased in Lycium barbarum L.,while nitrogen fertilizer applicationdelayed,otherwise,too much nitrogen in the early or late growing stages.Application nitrogen evenly contributed to accumulation of betaine-aldehyde dehydrogenase(BADH) during growing season.The betaine content was directly related to the amount of nitrogen applied at different times,and the content was higher along with the nitrogen applied higher.The yield of Lycium barbarum L.was the highest at 2∶1∶1 with the ratio of nitrogen fertilizer applied,which was 276 kg per 667 m~2(dry weight).
引文
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[4] 高秀萍,闰继耀,刘恩科,等.水分胁迫下梨、枣和葡萄叶片中甜菜碱含量的变化[J].园艺学报,2002,29(3):268-270.
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[6] 陈少良,李金克,毕望富,等.盐胁迫条件下杨树盐分与甜菜碱及糖类物质变化[J].植物学通报,2001,18(5):587-596.
[7] WANG Y M,MENG Y L,NII N.Changes in glycine betaine and relatedenzyme contents in Amaranthus tricolor under salt stress[J].Plant Physiology and Molecular Biology,2004,30(5):496-502.
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[9] 明鹤,杨太新,杜艳华.不同采收时间枸杞子中枸杞多糖和甜菜碱含量的分析[J].时珍国医国药,2014,25(4):945-946.
[10] 康建宏,吴宏亮,杨涓,等.不同施氮水平下枸杞主要次生代谢产物与多糖的关系研究[J].安徽农业科学,2008,36(36):16008-16010.
[11] 张自萍,史晓文,郭荣,等.杞糖碱成分及其与土壤肥力间的关系研究[J].干旱地区农业研究,2008,11,26(6):149-153.
[12] 董锋.宁夏引黄灌区耕地土壤盐渍化调查与坑盐植物选育[M].银川:宁夏人民出版社,2006.
[13] 张晓煜,刘静,袁海燕,等.枸杞总糖含量与环境因子的量化关系研究[J].中国生态农业学报,2005,13(3):101-104.
[14] 贺春燕.施肥对枸杞产量和品质的影响及将就研究[D].兰州:甘肃农业大学,2009.
[15] 骆爱玲,王继伟,李佳格.一种简便的蛋白质免疫印渍法介绍[J].植物学通报,1995,12(2):63-64.
[16] 梁峥,马德钦,汤岚.菠菜甜菜碱醛脱氢酶基因在烟草中的表达[J].生物工程学报,1997,13(3):236-240.
[2] WYN J R G,STOREY R.Physiology and biochemistry of droughtresistance in plants[M].New York:Academic Press,1981:171-204.
[3] 赵勇,马雅琴,翁跃进.盐胁迫下小麦甜菜碱和脯氨酸含量变化[J].植物生理与分子生物学学报,2005,31(1):103-106.
[4] 高秀萍,闰继耀,刘恩科,等.水分胁迫下梨、枣和葡萄叶片中甜菜碱含量的变化[J].园艺学报,2002,29(3):268-270.
[5] REDDY A R,CHAITANYA K V,JUTUR P P,et al.Differential antioxidativeresponses to water stress among five mulberry(Morus alba L.)cuhivars[J].Environmental and Experimental Botany,2004,52(1):33-42.
[6] 陈少良,李金克,毕望富,等.盐胁迫条件下杨树盐分与甜菜碱及糖类物质变化[J].植物学通报,2001,18(5):587-596.
[7] WANG Y M,MENG Y L,NII N.Changes in glycine betaine and relatedenzyme contents in Amaranthus tricolor under salt stress[J].Plant Physiology and Molecular Biology,2004,30(5):496-502.
[8] ROBINSON S P,JONES G P.Accumulation of glycinebetaine in chloroplastsprovides osmotic adjustment during salt stress[J].Aust J PlantPhysiol,1986,13:659-668.
[9] 明鹤,杨太新,杜艳华.不同采收时间枸杞子中枸杞多糖和甜菜碱含量的分析[J].时珍国医国药,2014,25(4):945-946.
[10] 康建宏,吴宏亮,杨涓,等.不同施氮水平下枸杞主要次生代谢产物与多糖的关系研究[J].安徽农业科学,2008,36(36):16008-16010.
[11] 张自萍,史晓文,郭荣,等.杞糖碱成分及其与土壤肥力间的关系研究[J].干旱地区农业研究,2008,11,26(6):149-153.
[12] 董锋.宁夏引黄灌区耕地土壤盐渍化调查与坑盐植物选育[M].银川:宁夏人民出版社,2006.
[13] 张晓煜,刘静,袁海燕,等.枸杞总糖含量与环境因子的量化关系研究[J].中国生态农业学报,2005,13(3):101-104.
[14] 贺春燕.施肥对枸杞产量和品质的影响及将就研究[D].兰州:甘肃农业大学,2009.
[15] 骆爱玲,王继伟,李佳格.一种简便的蛋白质免疫印渍法介绍[J].植物学通报,1995,12(2):63-64.
[16] 梁峥,马德钦,汤岚.菠菜甜菜碱醛脱氢酶基因在烟草中的表达[J].生物工程学报,1997,13(3):236-240.