C-fos基因在麻醉中的功能分析及相关调控网络的构建
|
摘要
目的探讨C-fos基因的生物学功能,构建其在痛觉传递和调制机制中的调控网络。方法利用Gene Ontology(GO)和Kyoto Encyclopedia of Genes and Genomes(KEGG)数据库对C-fos基因进行功能注释和通路分析。结果 GO分析结果显示,C-fos在细胞组分、分子功能、生物学过程中均发挥重要作用,同时结合KEGG数据库成功绘制出以C-fos基因为中心的麻醉相关基因的调控网络。结论通过对C-fos基因在痛觉传递和调制机制过程中的生物学功能分析,可为C-fos基因在麻醉中功能研究方向提供理论基础和指导作用。
Objective To explore the biological function of C-fos gene and to construct its regulatory networks in the mechanism of pain transmission and modulation.Methods Functional annotation and pathway analysis of C-fos gene were performed using Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) databases.Results The results of GO analysis showed that C-fos played an important role in cellular components,molecular functions and biological processes.At the same time,combined with the KEGG database,a regulatory network map of anesthesia-related genes centering on the C-fos gene was successfully drawn.Conclusion This study provides a basis and guidance for the functional research of C-fos gene in anesthesia by analyzing the biological function of C-fos gene in the process of pain transmission and modulation.
Objective To explore the biological function of C-fos gene and to construct its regulatory networks in the mechanism of pain transmission and modulation.Methods Functional annotation and pathway analysis of C-fos gene were performed using Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) databases.Results The results of GO analysis showed that C-fos played an important role in cellular components,molecular functions and biological processes.At the same time,combined with the KEGG database,a regulatory network map of anesthesia-related genes centering on the C-fos gene was successfully drawn.Conclusion This study provides a basis and guidance for the functional research of C-fos gene in anesthesia by analyzing the biological function of C-fos gene in the process of pain transmission and modulation.
引文
[1] 李莉,余剑波,刘志学.针刺镇痛与c-fos基因的研究[J].临床麻醉学杂志,2011,27(10):1030-1032.
[2] DALLA MASSARA L,OSURU H P,OKLOPCIC A A,et al.General anesthesia causes epigenetic histone modulation of c-Fos and brain-derived neurotrophic factor,target genes important for neuronal development in the immature rat hippocampus[J].Anesthesiology,2016,124(6):1311-1327.
[3] SHEHAB S,D’SOUZA C,LJUBISAVLJEVIC M,et al.Activation of the subthalamic nucleus suppressed by high frequency stimulation:A c-Fos immunohistochemical study[J].Brain Res,2018,1685:42-50.
[4] GUPTA R G,SCHAFER C,RAMAROSON Y,et al.Role of the abdominal vagus and hindbrain in inhalational anesthesia-induced vomiting[J].Auton Neurosci,2017,202:114-121.
[5] YOON J Y,BAEK C W,KIM H J,et al.Remifentanil negatively regulates RANKL-induced osteoclast differentiation and bone resorption by inhibiting c-Fos/NFATc1 expression[J].TERM,2018,15(3):330-340.
[6] 胡越,刘莉,熊威威,等。电针复合七氟烷麻醉对断尾大鼠急性应激反应调控的影响[J].重庆医学,2016,45(10):1306-1308.
[7] LI X,LU F,LI W,et al.Underlying mechanisms of memory deficits induced by etomidate anesthesia in aged rat model:critical role of immediate early genes[J].Chinese Medical Journal,2016,129(1):48-53.
[8] HORNSBY A K,REDHEAD Y T,REES D J,et al.Short-term calorie restriction enhances adult hippocampal neurogenesis and remote fear memory in a Ghsr-dependent manner[J].Psychoneuroendocrinology,2016,63:198-207.
[9] YAN F,XIN W,CHEN S,et al.Role of the RVM in descending pain regulation originating from the cerebrospinal fluid-contacting nucleus[J].Neurochem Res,2016,41(7):1651-1661.
[10] MAHMOUD A D,LEWIS S,JURI,et al.AMP-activated protein kinase deficiency blocks the hypoxic ventilatory response and thus precipitates hypoventilation and apnea[J].Am J Respir Crit Care Med,2016,193(9):1032-1043.
[11] 王丹,王爱桃,姚尚龙,等.CaM/CaMK-Ⅱ信号通路在小鼠炎性痛中的作用[J].临床麻醉学杂志,2017(12):1207-1210.
[12] HERRERA C G,CADAVIECO M C,JEGO S,et al.Hypothalamic feedforward inhibition of thalamocortical network controls arousal and consciousness[J].Nat Neurosci,2016,19(2):290-298.
[13] KOVACS K J,SATOMOTO M,ADACHI Y U,et al.Apocynin preserves glutamatergic neurons in the basolateral amygdala in mice with neonatal sevoflurane exposure[J].Korean J Anesthesiol,2017,70(3):335-340.
[14] JEON H O,CHOI I S,YOON J Y,et al.Effect of remifentanil on pre-osteoclast cell differentiation in vitro[J].J Dent Anesth Pain Med,2018,18(1):9-17.
[15] 石涛."和解止痛颗粒"对偏头痛大鼠模型c-fos、c-jun基因表达影响的研究[D].长春:长春中医药大学,2016.
[16] WANG L,GUO T Z,WEI T,et al.Bisphosphonates inhibit pain,bone loss,and inflammation in a rat tibia fracture model of complex regional pain syndrome[J].Anesth Analg,2016,123(4):1033-1045.
[17] GALYFOS G,FILIS K,SIGALA F,et al.Beta-blockers in vascular surgery patients:is the debate still going on?[J].J Anesth,2016,30(6):1031-1036.
[18] 刘金平.c-fos基因在麻醉药麻醉机理研究中的应用[J].饲料与畜牧,2017(10):42-45.
[19] ELIAVA M,MELCHIOR M,KNOBLOCH BOLLMANN H S,et al.A new population of parvocellular oxytocin neurons controlling magnocellular neuron activity and inflammatory pain processing[J].Neuron,2016,89(6):1291-1304.
[20] HUHE M,LIU S,YANG Z,et al.Expression levels of transcription factors c-Fos and c-Jun and transmembrane protein HAb18G/CD147 in urothelial carcinoma of the bladder[J].Mol Med Rep,2017,15(5):2991-3000.
[2] DALLA MASSARA L,OSURU H P,OKLOPCIC A A,et al.General anesthesia causes epigenetic histone modulation of c-Fos and brain-derived neurotrophic factor,target genes important for neuronal development in the immature rat hippocampus[J].Anesthesiology,2016,124(6):1311-1327.
[3] SHEHAB S,D’SOUZA C,LJUBISAVLJEVIC M,et al.Activation of the subthalamic nucleus suppressed by high frequency stimulation:A c-Fos immunohistochemical study[J].Brain Res,2018,1685:42-50.
[4] GUPTA R G,SCHAFER C,RAMAROSON Y,et al.Role of the abdominal vagus and hindbrain in inhalational anesthesia-induced vomiting[J].Auton Neurosci,2017,202:114-121.
[5] YOON J Y,BAEK C W,KIM H J,et al.Remifentanil negatively regulates RANKL-induced osteoclast differentiation and bone resorption by inhibiting c-Fos/NFATc1 expression[J].TERM,2018,15(3):330-340.
[6] 胡越,刘莉,熊威威,等。电针复合七氟烷麻醉对断尾大鼠急性应激反应调控的影响[J].重庆医学,2016,45(10):1306-1308.
[7] LI X,LU F,LI W,et al.Underlying mechanisms of memory deficits induced by etomidate anesthesia in aged rat model:critical role of immediate early genes[J].Chinese Medical Journal,2016,129(1):48-53.
[8] HORNSBY A K,REDHEAD Y T,REES D J,et al.Short-term calorie restriction enhances adult hippocampal neurogenesis and remote fear memory in a Ghsr-dependent manner[J].Psychoneuroendocrinology,2016,63:198-207.
[9] YAN F,XIN W,CHEN S,et al.Role of the RVM in descending pain regulation originating from the cerebrospinal fluid-contacting nucleus[J].Neurochem Res,2016,41(7):1651-1661.
[10] MAHMOUD A D,LEWIS S,JURI,et al.AMP-activated protein kinase deficiency blocks the hypoxic ventilatory response and thus precipitates hypoventilation and apnea[J].Am J Respir Crit Care Med,2016,193(9):1032-1043.
[11] 王丹,王爱桃,姚尚龙,等.CaM/CaMK-Ⅱ信号通路在小鼠炎性痛中的作用[J].临床麻醉学杂志,2017(12):1207-1210.
[12] HERRERA C G,CADAVIECO M C,JEGO S,et al.Hypothalamic feedforward inhibition of thalamocortical network controls arousal and consciousness[J].Nat Neurosci,2016,19(2):290-298.
[13] KOVACS K J,SATOMOTO M,ADACHI Y U,et al.Apocynin preserves glutamatergic neurons in the basolateral amygdala in mice with neonatal sevoflurane exposure[J].Korean J Anesthesiol,2017,70(3):335-340.
[14] JEON H O,CHOI I S,YOON J Y,et al.Effect of remifentanil on pre-osteoclast cell differentiation in vitro[J].J Dent Anesth Pain Med,2018,18(1):9-17.
[15] 石涛."和解止痛颗粒"对偏头痛大鼠模型c-fos、c-jun基因表达影响的研究[D].长春:长春中医药大学,2016.
[16] WANG L,GUO T Z,WEI T,et al.Bisphosphonates inhibit pain,bone loss,and inflammation in a rat tibia fracture model of complex regional pain syndrome[J].Anesth Analg,2016,123(4):1033-1045.
[17] GALYFOS G,FILIS K,SIGALA F,et al.Beta-blockers in vascular surgery patients:is the debate still going on?[J].J Anesth,2016,30(6):1031-1036.
[18] 刘金平.c-fos基因在麻醉药麻醉机理研究中的应用[J].饲料与畜牧,2017(10):42-45.
[19] ELIAVA M,MELCHIOR M,KNOBLOCH BOLLMANN H S,et al.A new population of parvocellular oxytocin neurons controlling magnocellular neuron activity and inflammatory pain processing[J].Neuron,2016,89(6):1291-1304.
[20] HUHE M,LIU S,YANG Z,et al.Expression levels of transcription factors c-Fos and c-Jun and transmembrane protein HAb18G/CD147 in urothelial carcinoma of the bladder[J].Mol Med Rep,2017,15(5):2991-3000.