野生与栽培太白米HPLC指纹图谱及化学模式识别
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摘要
目的:建立野生和栽培太白米的HPLC指纹图谱,结合化学模式识别方法对二者进行判别,以期为不同来源太白米药材的质量评价和驯化栽培提供依据。方法:利用高效液相色谱法,测定10批野生品和10批栽培品共计20批太白米药材的指纹图谱,建立指纹图谱共有模式,采用中药色谱指纹图谱相似度评价系统(2012A版)计算相似度,运用主成分分析(PCA),聚类分析(HCA)和最小偏二乘法-判别分析(PLS-DA)对野生与栽培品太白米进行模式识别研究。结果:建立了野生与栽培太白米的HPLC指纹图谱共有模式,标定了26个共有峰,20批太白米样品的指纹图谱与对照指纹图谱相似度> 0. 9。主成分分析、聚类分析和最小偏二乘法-判别分析能对野生与栽培太白米进行明确的区分,表明导致差异的主要化学标记物为对香豆酸等8个成分。结论:该文建立的太白米HPLC指纹图谱具有较强的特征性和重复性,与模式识别方法结合起来可有效的评价太白米质量以及区分其野生与栽培品,为太白米的质量控制和驯化栽培提供参考依据。
Objective: To establish the HPLC fingerprint of wild and cultivated Notholirion bulbuliferum,and recognize them according to the chemical pattern,in the expectation of providing the basis for the quality control and domestication cultivation of N. bulbuliferum of origins. Method: Twenty samples of wild and cultivated N. bulbuliferum collected from different origins were detected by HPLC,and a common mode of fingerprint was established. The Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 A edition) was used to evaluate the similarity of the samples. The differences among the samples were identified by chemical pattern recognition methods,including principal component analysis(PCA),cluster analysis(HCA) and partial least squares discriminate analysis(PLS-DA). Result: The HPLC fingerprint of N. bulbuliferum was obtained,and 26 common peaks were found in the chromatograph. Similarities of all samples were over 0. 9,PCA,and HCA and PLS-DA results demonstrated obvious distinctions between wild and cultivated N. bulbuliferum. Eight constituents,such as pcoumaric acid were identified as biomarkers,representing major differences between the two varieties.Conclusion: The HPLC chromatogram of N. bulbuliferum developed in this paper has strong characteristics and repeatability. After being combined with the pattern recognition mode,it can be used as an effective method for evaluating the quality of N. bulbuliferum and distinguishing wild and cultivated N. bulbuliferum,and provide a reference for the quality control and domestication introduction of N. bulbuliferum.
Objective: To establish the HPLC fingerprint of wild and cultivated Notholirion bulbuliferum,and recognize them according to the chemical pattern,in the expectation of providing the basis for the quality control and domestication cultivation of N. bulbuliferum of origins. Method: Twenty samples of wild and cultivated N. bulbuliferum collected from different origins were detected by HPLC,and a common mode of fingerprint was established. The Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 A edition) was used to evaluate the similarity of the samples. The differences among the samples were identified by chemical pattern recognition methods,including principal component analysis(PCA),cluster analysis(HCA) and partial least squares discriminate analysis(PLS-DA). Result: The HPLC fingerprint of N. bulbuliferum was obtained,and 26 common peaks were found in the chromatograph. Similarities of all samples were over 0. 9,PCA,and HCA and PLS-DA results demonstrated obvious distinctions between wild and cultivated N. bulbuliferum. Eight constituents,such as pcoumaric acid were identified as biomarkers,representing major differences between the two varieties.Conclusion: The HPLC chromatogram of N. bulbuliferum developed in this paper has strong characteristics and repeatability. After being combined with the pattern recognition mode,it can be used as an effective method for evaluating the quality of N. bulbuliferum and distinguishing wild and cultivated N. bulbuliferum,and provide a reference for the quality control and domestication introduction of N. bulbuliferum.
引文
[1]陕西省食品药品监督管理局.陕西省药材标准[M].西安:陕西科学技术出版社,2016:135-136.
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[4]邵囡囡,杨吉安,刘建军,等.秦岭太白米中黄酮类成分的研究[J].西北林学院学报,2011,26(5):166-169.
[5]李朋朋.太白米及其提取物治疗慢性胃炎物质基础及机制研究[D].西安:陕西中医学院,2014.
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[8]宋利伟,张存旭,康永祥,等.太白米离体愈伤组织诱导及植株再生[J].东北林业大学学报,2014,42(9):107-110,115.
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[11]胡本祥,王继涛,张琳,等.太白米濒危因素研究之一—太白米植物的生活习性及其资源分布调查研究[J].陕西中医学院学报,2007,30(6):49-52.
[12]胡本祥,张琳.太白米的研究进展[J].中草药,2006,37(2):312-314.
[13]胡本祥.太白米栽培技术研究[J].中草药,2004,35(1):100-101.
[14]谢丽.太白米的质量标准及指纹图谱研究[D].西安:陕西中医学院,2012.
[15]孙立丽,王萌,任晓亮.化学模式识别方法在中药质量控制研究中的应用进展[J].中草药,2017,48(20):4339-4345.
[16]皮胜玲,胡玉珍,彭曦,等.野生与栽培夏枯草HPLC指纹图谱研究及模式识别分析[J].中国药学杂志,2017,52(5):367-371.
[17]肖榕,林艳,雷思敏,等. UPLC指纹图谱结合模式识别分析不同产地生/制何首乌[J].中国中药杂志,2017,42(12):2305-2310.
[18]刘静,苏建春,甘林建,等.鸡血藤UPLC指纹图谱及模式识别研究[J].中国实验方剂学杂志,2014,20(4):87-90.
[19]尹海波,康廷国,张囡,等.穿龙薯蓣HPLC指纹图谱及模式识别[J].中国实验方剂学杂志,2012,18(22):145-148.
[20]杨天鸣,张璐,付海燕,等.不同产地甘草的近红外指纹图谱模式识别鉴别方法[J].亚太传统医药,2015,11(14):11-14.
[21]胡玉珍,周亚敏,熊苏慧,等. UPLC结合化学计量学方法的野生与市售山楂叶指纹图谱研究[J].中药材,2017,40(6):1355-1359.
[2]邱芳龙,陈昌祥,畅行若,等.太白米的甾体生物碱甙(一)[J].云南植物研究,1982,4(4):419-423.
[3]屠鹏飞,吴卫中,郑俊华.太白米的酚酸类成分研究[J].药学学报,1999,34(1):40-43.
[4]邵囡囡,杨吉安,刘建军,等.秦岭太白米中黄酮类成分的研究[J].西北林学院学报,2011,26(5):166-169.
[5]李朋朋.太白米及其提取物治疗慢性胃炎物质基础及机制研究[D].西安:陕西中医学院,2014.
[6]周乐,董旭俊,赵海双,等.太白米中的甾体生物碱苷[J].西北植物学报,2005,25(6):1158-1162.
[7]车乐,曹博,白成科,等.基于MaxEnt和Arc GIS对太白米的潜在分布预测及适宜性评价[J].生态学杂志,2014,33(6):1623-1628.
[8]宋利伟,张存旭,康永祥,等.太白米离体愈伤组织诱导及植株再生[J].东北林业大学学报,2014,42(9):107-110,115.
[9]邵囡囡.秦岭太白米组织培养快繁技术及总黄酮成分的研究[D].杨凌:西北农林科技大学,2011.
[10]赵银萍,高志强,董忠民.濒危中药材太白米组织培养及总黄酮含量测定[J].西北植物学报,2010,30(1):175-179.
[11]胡本祥,王继涛,张琳,等.太白米濒危因素研究之一—太白米植物的生活习性及其资源分布调查研究[J].陕西中医学院学报,2007,30(6):49-52.
[12]胡本祥,张琳.太白米的研究进展[J].中草药,2006,37(2):312-314.
[13]胡本祥.太白米栽培技术研究[J].中草药,2004,35(1):100-101.
[14]谢丽.太白米的质量标准及指纹图谱研究[D].西安:陕西中医学院,2012.
[15]孙立丽,王萌,任晓亮.化学模式识别方法在中药质量控制研究中的应用进展[J].中草药,2017,48(20):4339-4345.
[16]皮胜玲,胡玉珍,彭曦,等.野生与栽培夏枯草HPLC指纹图谱研究及模式识别分析[J].中国药学杂志,2017,52(5):367-371.
[17]肖榕,林艳,雷思敏,等. UPLC指纹图谱结合模式识别分析不同产地生/制何首乌[J].中国中药杂志,2017,42(12):2305-2310.
[18]刘静,苏建春,甘林建,等.鸡血藤UPLC指纹图谱及模式识别研究[J].中国实验方剂学杂志,2014,20(4):87-90.
[19]尹海波,康廷国,张囡,等.穿龙薯蓣HPLC指纹图谱及模式识别[J].中国实验方剂学杂志,2012,18(22):145-148.
[20]杨天鸣,张璐,付海燕,等.不同产地甘草的近红外指纹图谱模式识别鉴别方法[J].亚太传统医药,2015,11(14):11-14.
[21]胡玉珍,周亚敏,熊苏慧,等. UPLC结合化学计量学方法的野生与市售山楂叶指纹图谱研究[J].中药材,2017,40(6):1355-1359.